| Budget Amount *help |
¥166,270,000 (Direct Cost: ¥127,900,000、Indirect Cost: ¥38,370,000)
Fiscal Year 2017: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Fiscal Year 2016: ¥30,160,000 (Direct Cost: ¥23,200,000、Indirect Cost: ¥6,960,000)
Fiscal Year 2015: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Fiscal Year 2014: ¥30,160,000 (Direct Cost: ¥23,200,000、Indirect Cost: ¥6,960,000)
Fiscal Year 2013: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
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| Outline of Final Research Achievements |
We have studied the profiles and roles of epigenomic modifications involved in genomic imprinting and transposon repression during mammalian gametogenesis and early development. We found that: histone H3K9 methylation, which is known to be important for transposon repression, is essential for germ cell development; the mechanism of transposon repression switches from RNA degradation to DNA methylation during spermatogenesis; the profiles of DNA methylation and gene expression change upon spermatogonial stem cell formation; a factor responsible for DNA methylation maintenance in somatic cells is involved in de novo methylation in oocytes; primordial germ cell-like cells in culture, which provide an important source for biochemical analyses of germ cells, recapitulate the DNA methylation and gene expression profiles of their in vivo counterparts. Our results provide important insights into the genomic and epigenomic regulation of gametogenesis and early development.
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