| Budget Amount *help |
¥167,050,000 (Direct Cost: ¥128,500,000、Indirect Cost: ¥38,550,000)
Fiscal Year 2017: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2016: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2015: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2014: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2013: ¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
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| Outline of Final Research Achievements |
In this research, we aimed to clarify gene networks regulating conversion between primordial germ cells (PGCs) and pluripotential stem cells (PSCs) and their possible evolutional conservation, and have reached five major conclusions. 1) Max cooperatively represses germ cell-specific genes with DNA methyltransferase (DNMTs) and histone H3K9 methyltransferase (SETDB1) in ES cells. 2) A RNA binding protein, DND1 represses conversion of PGCs into PSCs by maintaining the expression of H3K27 methyltransferase (Ezh2) via inhibiting a micro RNA. 3) PGCs and PSCs have distinct energy metabolic status. 4) A histone modifying enzyme gene identified by a siRNA screening is essential for PGC specification from epiblast via repressing somatic gene expression. 5) Max does not play a major role for repression of germ cell-specific genes in planarian, chick and zebrafish.
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