| Budget Amount *help |
¥172,900,000 (Direct Cost: ¥133,000,000、Indirect Cost: ¥39,900,000)
Fiscal Year 2017: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2016: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2015: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2014: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2013: ¥53,040,000 (Direct Cost: ¥40,800,000、Indirect Cost: ¥12,240,000)
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| Outline of Final Research Achievements |
We introduced microfluidic technologies to our testis organ culture method to develop a new culture system. The microfluidic devices we produced were successfully maintained structure and function of mouse testis tissues over 6 months and kept producing sperm. Offspring was produced with them by micro-insemination. We also simplified the devices for its general use and popularization. As for culture medium, we formulated a chemically-defined medium which induced mouse spermatogenesis up to haploid cell formation. Retinoic acid, lipids, and hormones were found to be important for in vitro spermatogenesis.
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