| Budget Amount *help |
¥232,700,000 (Direct Cost: ¥179,000,000、Indirect Cost: ¥53,700,000)
Fiscal Year 2017: ¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Fiscal Year 2016: ¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Fiscal Year 2015: ¥37,180,000 (Direct Cost: ¥28,600,000、Indirect Cost: ¥8,580,000)
Fiscal Year 2014: ¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Fiscal Year 2013: ¥78,130,000 (Direct Cost: ¥60,100,000、Indirect Cost: ¥18,030,000)
|
|---|
| Outline of Final Research Achievements |
In eukaryotes, genomic DNA is compacted as chromatin, which is formed as the higher ordered protein-DNA complex. Recent evidences suggested that versatility of the structures and dynamics of chromatin is essential for gene regulation. However, the mechanism by which the chromatin dynamics regulates eukaryotic genes has not been elucidated yet. The purpose of this study is to elucidate the structures and dynamics of fundamental unites of chromatin. To do so, we have established in vitro methods for chromatin reconstitution. We then performed X-ray crystallography, X-ray and neutron scattering, and cryogenic electron microscopy with the reconstituted chromatin samples. We also performed the biochemical, cell biological, and genomics approaches. We then revealed many important aspects of chromatin to understand the molecular mechanism for genomic DNA function in eukaryotes.
|
