| Budget Amount *help |
¥52,260,000 (Direct Cost: ¥40,200,000、Indirect Cost: ¥12,060,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2013: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Outline of Final Research Achievements |
We studied impact of post-translational modifications of histones and histone variants on the structure and dynamics of mono-nucleosomes and poly-nucleosomes by integrating computer simulation and experimental data. We found that acetylation and methylation of H3 tails induce DNA dissociation from the histone core. We determined a novel nucleosome structure which is overlapping the typical 8-mer nucleosome and a 6-mer nucleosome which is missing one H2A-H2B heterodimer from the typical nucleosome. In addition, we found H4 tails play an important role in producing various packing forms of two nucleosomes, indicating that the way of the tail interactions yields a variety of chromatin conformations.
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