| Budget Amount *help |
¥88,660,000 (Direct Cost: ¥68,200,000、Indirect Cost: ¥20,460,000)
Fiscal Year 2017: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2016: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2015: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2014: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2013: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
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| Outline of Final Research Achievements |
For the purpose of understanding the molecular mechanism of chromatin functions, we have developed integrated microscopy with improved resolution in multi-color single-molecule super-resolution microscopy by optimizing illumination and stabilization of optics. We have also developed a new method, moving subtrajectory analysis using single-molecule tracking, which provides us spatiotemporal to quantification of not only dynamics but also the kinetics of interactions using single-color images. Combined use of this with multi-color simultaneous imaging and super-resolution microscopy, enables integrated analysis of different kinds of molecules with a spatiotemporal resolution and in relation to cellular activity. Using these techniques, we carried out imaging analysis of molecular dynamics and interactions of nucleosomes, RNA polymerase II, histone variants, transcription regulatory proteins, the INO80 chromatin remodeling complex, and the multilayer structure of the nucleosome.
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