|Budget Amount *help
¥123,500,000 (Direct Cost: ¥95,000,000、Indirect Cost: ¥28,500,000)
Fiscal Year 2017: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2016: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2015: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2014: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2013: ¥29,640,000 (Direct Cost: ¥22,800,000、Indirect Cost: ¥6,840,000)
|Outline of Final Research Achievements
In the nucleus, chromatin is surrounded by several kinds of sub-structures. The nuclear sub-structures are supramolecular complexes composed of RNA and proteins. Factors involved in nuclear events are locally concentrated in the sub-structures.
The goal of this study was to elucidate the mechanisms of how chromatin is regulated in the context of the three-dimensional nuclear space. We analyzed factors linking chromatin and sub-structures. As a result, we found novel nuclear non-coding RNAs that are involved in acquisition of endocrine therapy-resistance of breast cancer cells. The ncRNAs form RNA cloud, and they are involved in gene expression regulation essential for breast cancer cells. We also identified and analyzed proteins involved in structure and function of the nucleolus. We found that interplay between nuclear sub-structures and chromatin is important for regulation of chromatin structure and dynamics.