Control of synchronous activity of neurons by astrocytes and their functions

• Project Area: Glial assembly: a new regulatory machinery of brain function and disorders
• Project/Area Number: 25117004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo (2016-2017); Kyushu University (2013-2015)
• Principal Investigator: Ohki Kenichi 東京大学, 大学院医学系研究科(医学部), 教授 (50332622)
• Co-Investigator(Renkei-kenkyūsha): KONDO Satoru 東京大学, 大学院医学系研究科, 講師 (20301757)
• Project Period (FY): 2013-06-28 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥79,950,000 (Direct Cost: ¥61,500,000、Indirect Cost: ¥18,450,000); Fiscal Year 2017: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2016: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2015: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2014: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
• Keywords: astrocyte / neuron / interaction / activity / calcium / アストロサイト / カルシウム / ２光子イメージング / 同期活動 / 大脳皮質 / 視覚野 / 神経細胞 / 2光子イメージング / DREADD / 方位選択性

Outline of Final Research Achievements

We examined how astrocytes affect neuronal activity in developing mice and adult mice. In developing mice, astrocyte calcium activity enhances synchronous neuronal activity. In mice with little astrocyte activity, functional maturation of neurons in visual cortex is significantly suppressed. In adult mice, astrocyte calcium activity suppresses synchronous neuronal activity. Further, synchronous neuronal activity evokes astrocyte calcium activity and blood flow change similar to the pattern of neuronal activity.

Research Products

• [Journal Article] Neuronal Origin of the Temporal Dynamics of Spontaneous BOLD Activity Correlation. (2018)
  • Author(s): T. Matsui, T. Murakami, K. Ohki
  • Journal Title: Cerebral Cortex Volume: in press Issue: 4 Pages: 4500-5000
  • DOI: 10.1093/cercor/bhy045
  • Peer Reviewed
• [Journal Article] Functional Segregation and Development of Mouse Higher Visual Areas (2017)
  • Author(s): T. Murakami, T. Matsui, K. Ohki
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 39 Pages: 9424-9437
  • DOI: 10.1523/jneurosci.0731-17.2017
  • Peer Reviewed / Open Access
• [Journal Article] Color Representation Is Retinotopically Biased but Locally Intermingled in Mouse V1. (2017)
  • Author(s): Aihara S, Yoshida T, Hashimoto T, Ohki K.
  • Journal Title: Front Neural Circuits. Volume: 11 Pages: 22-22
  • DOI: 10.3389/fncir.2017.00022
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transient neuronal coactivations embedded in globally propagating waves underlie resting-state functional connectivity. (2016)
  • Author(s): Matsui T, Murakami T, Ohki K.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 23 Pages: 6556-61
  • DOI: 10.1073/pnas.1521299113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mixed functional microarchitectures for orientation selectivity in the mouse primary visual cortex. (2016)
  • Author(s): Kondo S, Yoshida T, Ohki K.
  • Journal Title: Nat Commun. Volume: 7 Issue: 1 Pages: 13210-13210
  • DOI: 10.1038/ncomms13210
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Laminar differences in the orientation selectivity of geniculate afferents in mouse primary visual cortex. (2016)
  • Author(s): Kondo S, Ohki K.
  • Journal Title: Nat Neurosci. Volume: 19 Issue: 2 Pages: 316-9
  • DOI: 10.1038/nn.4215
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transient neuronal co-activations embedded in globally propagating waves underlie resting-state functional connectivity. (2016)
  • Author(s): Matsui T, Murakami T, Ohki K.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Wide-field Ca(2+) imaging reveals visually evoked activity in the retrosplenial area (2015)
  • Author(s): 村上知成, 吉田盛史, 松井鉄平, 大木研一
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 8 Pages: 1-12
  • DOI: 10.3389/fnmol.2015.00020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Neuronal activity is not required for the initial formation and maturation of visual selectivity (2015)
  • Author(s): 萩原 賢太, 村上知成, 吉田盛史, 田川義晃, 大木研一
  • Journal Title: Ｎａｔｕｒｅ Ｎｅｕｒｏｓｃｉｅｎｃｅ Volume: 18 Issue: 12 Pages: 1780-1788
  • DOI: 10.1038/nn.4155
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In vivo two-photon calcium imaging in the visual system. (2014)
  • Author(s): Ohki K, Reid RC.
  • Journal Title: Cold Spring Harb Protoc. Volume: 4 Issue: 4 Pages: 402-16
  • DOI: 10.1101/pdb.prot081455
  • Peer Reviewed
• [Presentation] 神経活動と血流の大規模計測 (2018)
  • Author(s): Ohki K.
  • Organizer: Neurovascular Unit研究会2018
• [Presentation] Functional micro architecture of direction selectivity in the cat area 17. (2017)
  • Author(s): Matsui T, Nishiyama M, Murakami T, Ohki K.
  • Organizer: 第17回 東京大学生命科学シンポジウム
• [Presentation] Functional microarchitecture of direction selectivity in the cat area 17. (2017)
  • Author(s): Matsui T, Nishiyama M, Murakami T, Ohki K.
  • Organizer: 第40回神経科学大会
• [Presentation] Multiscale Calcium Imaging in Visual Cortex of Marmoset Monkeys. (2017)
  • Author(s): Hashimoto T, Uemura M, Matsui T, Murakami T, Ohki K
  • Organizer: 第40回神経科学大会
• [Presentation] Brain-wide mapping of stimulus induced variability quenching reveals modularity of cortical networks. (2017)
  • Author(s): Matsui T, Murakami T, Ohki K.
  • Organizer: Computational and Cognitive Neuroscience.
• [Presentation] マウス一次視覚野の方位選択性に関する神経回路の機能構築. (2017)
  • Author(s): Kondo S, Ohki K.
  • Organizer: 生理学研究所研究会「大脳皮質回路の機能原理を探る」
• [Presentation] Large-scale recording of neuronal activity in visual cortex. (2017)
  • Author(s): Ohki K.
  • Organizer: UT-NTU Joint Conference
• [Presentation] Spine density and cortical computation. (2017)
  • Author(s): Ohki K.
  • Organizer: The 48th NIPS International Symposium “Neural circuitry and plasticity underlying brain function”
• [Presentation] Laminar differences in the orientation-selectivity of LGN inputs to mouse V1. (2017)
  • Author(s): Kondo S, Ohki K.
  • Organizer: NIG Symposium 'Circuit Construction in the mammalian brain'
• [Presentation] Multi-scale, multi-modal imaging of the visual cortical network in marmosets. (2017)
  • Author(s): Matsui T, Hashimoto T, Uemura M, Murakami T, Kikuta K, Kato T, Ohki K.
  • Organizer: 第７回マーモセット研究会大会
• [Presentation] Large-scale recording of neuronal activity and hemodynamics. (2017)
  • Author(s): Ohki K.
  • Organizer: 第40回神経科学大会
• [Presentation] Alteration of transient neuronal coactivation pattern explains dynamic and static change of resting-state functional connectivity. (2016)
  • Author(s): 松井 鉄平、村上知成、大木研一
  • Organizer: 日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Visual Motion Processing in Mouse Higher Visual Areas (2016)
  • Author(s): 橋本昂之、吉田盛史、大木研一
  • Organizer: 日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Gap Junctions in Early Postnatal Excitatory Neurons Regulate Spine Density and Maturation of Response Reliability. (2016)
  • Author(s): Kenji Hayashi, Kenichi Ohki
  • Organizer: Annual meeting of Society for Neuroscience
  • Place of Presentation: San Diego
  • Int'l Joint Research
• [Presentation] Visual image reconstruction from neuronal activities in the mouse (2015)
  • Author(s): Yoshida T, Ohki K
  • Organizer: 45th annual meeting of Society for Nauroscience
  • Place of Presentation: Chicago
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Neuronal activity reorganizes orientation selectivity derived from innate circuits in visual cortex (2015)
  • Author(s): Ohki K
  • Organizer: 第38回神経科学大会
  • Place of Presentation: kobe
  • Year and Date: 2015-07-28
  • Invited
• [Presentation] Functional diversification in clonal cells after eye-opening in mouse visual cortex. (2015)
  • Author(s): Ohtsuki G, Lois C, Ohki K.
  • Organizer: 第38回神経科学大会
  • Place of Presentation: Kobe
  • Year and Date: 2015-07-28
• [Presentation] Interaction between stimulus-evoked and ongoing neocortical activity investigated with wide-field calcium imaging. (2015)
  • Author(s): Matsui T, Murakami T, Ohki K.
  • Organizer: 第38回神経科学大会
  • Place of Presentation: Kobe
  • Year and Date: 2015-07-28
• [Presentation] Top-down developmental progression of functional cortical network revealed by wide-field Ca2+ imaging. (2015)
  • Author(s): Murakami T, Matsui T, Ohki K.
  • Organizer: 第38回神経科学大会
  • Place of Presentation: Kobe
  • Year and Date: 2015-07-28
• [Presentation] Gabor filter-based image reconstruction from the population activity in mouse primary visual cortex. (2015)
  • Author(s): Yoshida T, Ohki K
  • Organizer: 第38回神経科学大会
  • Place of Presentation: Kobe
  • Year and Date: 2015-07-28
• [Presentation] Cell-type specific response to vibro-tactile stimuli in mouse S1 hind limb area. (2015)
  • Author(s): Hayashi A, Yoshida T, Ohki K.
  • Organizer: 第38回神経科学大会
  • Place of Presentation: Kobe
  • Year and Date: 2015-07-28
• [Presentation] Interplay between innate circuits and neuronal activity in the formation of orientation selectivity in visual cortex、 (2015)
  • Author(s): Ohki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-23
  • Invited
• [Presentation] In vivo calcium imaging of thalamocortical axons in mouse primary visual cortex (2015)
  • Author(s): Kondo S, Ohki K.
  • Organizer: 第９２回生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-23
• [Presentation] Decoding of visualimages from the population activity in mouse primary visual cortex (2014)
  • Author(s): Yoshida T, Ohki K
  • Organizer: Vision, Memory, Thought
  • Place of Presentation: 東京
  • Year and Date: 2014-12-06
• [Presentation] Neuronal basis of resting state functional connectivity investigated with simultaneous wide field imaging of intrinsic and calcium signal (2014)
  • Author(s): T. Matsui, T.Murakami, K. Ohki
  • Organizer: Vision, Memory, Thought
  • Place of Presentation: 東京
  • Year and Date: 2014-12-06
• [Presentation] Activity is required not for initial formation but for later reorganization of neuronal functions in visual cortex (2014)
  • Author(s): Hagihara K, Murakami T, Yoshida T, Tagawa Y, Ohki K
  • Organizer: Vision, Memory, Thought
  • Place of Presentation: 東京
  • Year and Date: 2014-12-06
• [Presentation] Activity is required not for initial formation but for later reorganization of neuronal functions in visual cortex、 (2014)
  • Author(s): Hagihara K, Murakami T, Yoshida T, Tagawa Y, Ohki K、
  • Organizer: 遺伝研シンポジウム「哺乳類脳の機能的神経回路の構築メカニズム」
  • Place of Presentation: 三島
  • Year and Date: 2014-12-01
• [Presentation] Response selectivity of thalamocortical projections in mouse primary visual cortex (2014)
  • Author(s): Kondo S, Ohki K
  • Organizer: Society for Neuroscience Annual Meeting
  • Place of Presentation: ワシントンDC
  • Year and Date: 2014-11-17
• [Presentation] Neuronal basis of resting-state functional connectivity investigated with simultaneous widefield imaging of intrinsic and calcium signals (2014)
  • Author(s): T. Matsui, T.Murakami, K. Ohki
  • Organizer: Society for Neuroscience Annual Meeting
  • Place of Presentation: ワシントンDC
  • Year and Date: 2014-11-17
• [Presentation] マクロとミクロをつなぐマルチモーダル機能マッピング技術の開発 (2014)
  • Author(s): 大木研一
  • Organizer: 第３回 蛍光イメージング・ミニシンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-24
  • Invited
• [Presentation] Neuronal basis of resting-state functional connectivity investigated with simultaneous widefield imaging of intrinsic and calcium signals (2014)
  • Author(s): T. Matsui, T.Murakami, K. Ohki
  • Organizer: Homeodynamics in Clocks, Sleep and Metabolism
  • Place of Presentation: 東京
  • Year and Date: 2014-09-24
• [Presentation] Reconstruction of stimulus images from the population activity in mouse primary visual cortex (2014)
  • Author(s): Yoshida T, Ohki K
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-13
• [Presentation] Interplay between innate circuits and neuronal activity in the formation of orientation selectivity in visual cortex (2014)
  • Author(s): Ohki K、
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-12
  • Invited
• [Presentation] Neuronal basis of resting-state functional connectivity investigated with simultaneous widefield imaging of intrinsic and calcium signals (2014)
  • Author(s): T. Matsui, T.Murakami, K. Ohki
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-12
• [Presentation] Response selectivity of thalamocortical axons in mouse primary visual cortex (2014)
  • Author(s): Kondo S, Ohki K
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11
• [Presentation] Activity is required not for initial formation but for later reorganization of neuronal functions in visual cortex (2014)
  • Author(s): Hagihara K, Tagawa Y, Yoshida T, Murakami T, Ohki K
  • Organizer: 9th FENS Forum of Neuroscience
  • Place of Presentation: ミラノ
  • Year and Date: 2014-07-06
• [Presentation] 大脳皮質の神経細胞の活動の網羅的な記録に向けて (2014)
  • Author(s): 大木研一
  • Organizer: 日本顕微鏡学会第70回記念学術講演会
  • Place of Presentation: 千葉
  • Year and Date: 2014-05-13
  • Invited
• [Presentation] 大脳皮質の神経細胞の活動の網羅的な記録に向けて (2014)
  • Author(s): 大木研一
  • Organizer: 自然科学研究機構 新分野創成センターシンポジウム「大規模脳神経回路機能マップのその先」
  • Place of Presentation: 東京
  • Invited
• [Presentation] マウス一次視覚野の３次元機能構築 (2014)
  • Author(s): 根東覚、大木研一
  • Organizer: 日本解剖学会総会
  • Place of Presentation: 下野（自治医科大学）
  • Invited
• [Presentation] 大脳皮質視覚野における非活動依存的機能形成と活動依存的機能再編成 (2014)
  • Author(s): 萩原賢太 田川義晃 吉田盛史 村上知成 大木研一
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] 大脳皮質視覚野の機能的神経回路の構築原理 (2013)
  • Author(s): 大木研一
  • Organizer: CREST / さきがけ 合同シンポジウム「感覚情報の脳内表現」
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] Brain development and neural plasticity. (2013)
  • Author(s): Ohki K.
  • Organizer: 欧米の若手研究者を招いての九州大学における精神神経免疫グリア研究発展のための合同セミナー
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Development of visual functions: nature vs nurture. (2013)
  • Author(s): Ohki K.
  • Organizer: The 3rd International Symposium by JSPS Core-to-Core Program “Cooperative International Framework in TGF-β Family Signaling"
  • Place of Presentation: 松山
  • Invited
• [Presentation] Neural activity in P10-15 is critical for callosal axon projections in the mouse cerebral cortex. (2013)
  • Author(s): 手束勇太 萩原賢太 平野丈夫 大木研一 田川義晃
  • Organizer: NIGシンポジウム「哺乳類脳の機能的神経回路の構築」
  • Place of Presentation: 三島（遺伝研）
• [Presentation] Silencing activity of individual neurons throughout development does not prevent maturation of orientation and direction selectivity in mouse visual cortex. (2013)
  • Author(s): Hagihara KM, Tagawa Y, Yoshida T, Murakami T, Ohki K.
  • Organizer: Annual meeting of Society for Neuroscience 2013
  • Place of Presentation: アメリカ サン・ディエゴ
• [Presentation] Functional Organization of Clonally Related Neurons in Mouse Visual Cortex during Development. (2013)
  • Author(s): Ohtsuki G, Lois C, Ohki K.
  • Organizer: Annual meeting of Society for Neuroscience 2013
  • Place of Presentation: アメリカ サン・ディエゴ
• [Remarks] 脳の神経活動の空間パターンは脳血流のパターンに写し取られる
  • URL: http://www.med.kyushu-u.ac.jp/app/modules/information/detail.php?i=862&c=10&s=20&k=
• [Remarks] 大脳視覚野の神経細胞が機能を獲得するメカニズムを解明 （分子生理学分野 大木研一教授）
  • URL: http://www.med.kyushu-u.ac.jp/app/modules/information/detail.php?i=816&c=10
• [Remarks] 物体の輪郭を認識する視覚のメカニズムの一端を解明 （分子生理学分野 大木研一教授）
  • URL: http://www.med.kyushu-u.ac.jp/app/modules/information/detail.php?i=829&c=10