| Co-Investigator(Kenkyū-buntansha) |
関口 博史 公益財団法人高輝度光科学研究センター, 利用研究促進部門, 主幹研究員 (00401563)
柴山 修哉 自治医科大学, 医学部, 教授 (20196439)
宮澤 淳夫 兵庫県立大学, 生命理学研究科, 教授 (60247252)
久保 泰 国立研究開発法人産業技術総合研究所, 創薬プロファイリング研究センター, 副研究センター長 (10178030)
一柳 光平 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 特任准教授 (70435618)
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| Budget Amount *help |
¥111,280,000 (Direct Cost: ¥85,600,000、Indirect Cost: ¥25,680,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2016: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2015: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥36,790,000 (Direct Cost: ¥28,300,000、Indirect Cost: ¥8,490,000)
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| Outline of Final Research Achievements |
This study aimed to develop two methodologies for biomaterials, fluorescent x-ray holography and x-ray single molecule tracking. In X-ray single molecule tracking method, gold nanocrystal labeling is realized by molecular labeling method targeting disulfide bond (SS), three-dimensional molecular dynamics measurement is succeeded by microsecond time division, and new kinetic measurement of membrane protein molecules succeeded in. We also proposed a completely new dynamic measurements without labeling. In fluorescence X-ray holography, we applied and measured fluorescence X-ray holography for the first time in the world for determining the 3D site around heavy elements of protein molecules through hemoglobin and myoglobin. The basic data of the main target photosynthetic protein group PSII, which is the final goal, could also be measured.
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