Formation and regulation of ligand signals associated with accepting and activating molecular oxygen

• Project Area: Oxygen biology: a new criterion for integrated understanding of life
• Project/Area Number: 26111011
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo (2016-2019); Nagoya University (2014-2015)
• Principal Investigator: UCHIDA Koji 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40203533)
• Co-Investigator(Kenkyū-buntansha): 西田 基宏 大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 教授 (90342641) ; 居原 秀 大阪府立大学, 理学(系)研究科(研究院), 教授 (60254447) ; 柴田 貴広 名古屋大学, 生命農学研究科, 准教授 (80447838)
• Project Period (FY): 2014-07-10 – 2019-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥113,750,000 (Direct Cost: ¥87,500,000、Indirect Cost: ¥26,250,000); Fiscal Year 2018: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000); Fiscal Year 2017: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000); Fiscal Year 2016: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000); Fiscal Year 2015: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000); Fiscal Year 2014: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
• Keywords: 活性酸素種 / ケミカルバイオロジー / タンパク質修飾 / リモデリング / レドックス

Outline of Final Research Achievements

(1) We identified a novel lysine adduct as an oxidative stress-related biomarker candidate. We also unambiguously detected 2-oxo-histidine-containing dipeptides in mouse tissue homogenates. In addition, an enhanced S-thiolation of HSA in the hyperlipidemia patients was observed.(2) We identified complement component C1q as a novel AGE-binding protein. We also discovered that the EGCG could be an endogenous source of the electrically-transformed proteins that could be recognized by the natural antibodies. In addition, lactate dehydrogenase was identified as a novel a mammalian PQQ-binding protein.(3) We found that the filamin A acted as a guanine nucleotide exchange factor for Drp1 and mediated mitochondrial fission-associated myocardial senescence in mice after myocardial infarction. We also demonstrate that TRPC3 acts as a positive regulator of ROS that stabilizes Nox2 activity through physical interaction, leading to amplification of maladaptive signaling in cardiomyocytes.

Academic Significance and Societal Importance of the Research Achievements

新規リジン付加体や2-オキソイミダゾールジペプチドに関する成果は、酸化ストレス関連疾患のメカニズム解明や予防・治療法開発が期待される新たな基盤となりうるものである。ポリフェノール類修飾タンパク質による生体防御機構に関する知見は、人類誕生より食されてきた植物成分の意義と人類進化のプロセスを解明する手がかりになりうる。
心筋組織におけるリモデリング機構に関する一連の研究成果は、病態マーカーの開発やリモデリング制御可能な医薬品などの応用開発に資することが期待される。また、慢性心不全の予後改善を可能とする既承認薬を同定したことで、ヒト健康社会の実現に寄与しうる臨床応用への糸口を見出した。

Research Products

• [Int'l Joint Research] Mahidol大学薬学部(タイ)
• [Int'l Joint Research] Sabah大学医学部(マレーシア)
• [Journal Article] A unique mechanism for thiolation of serum albumins by disulphide molecules (2019)
  • Author(s): Nakashima Fumie、Shibata Takahiro、Uchida Koji
  • Journal Title: The Journal of Biochemistry Volume: 167 Pages: 165-171
  • DOI: 10.1093/jb/mvz084
  • Open Access
• [Journal Article] Ibudilast attenuates doxorubicin‐induced cytotoxicity by suppressing formation of TRPC3 channel and NADPH oxidase 2 protein complexes (2019)
  • Author(s): Nishiyama Kazuhiro、Numaga‐Tomita Takuro、Fujimoto Yasuyuki、Tanaka Tomohiro、Toyama Chiemi、Nishimura Akiyuki、Yamashita Tomohiro、Matsunaga Naoya、Koyanagi Satoru、Azuma Yasu‐Taka、Ibuki Yuko、Uchida Koji、Ohdo Shigehiro、Nishida Motohiro
  • Journal Title: British Journal of Pharmacology Volume: 176 Issue: 18 Pages: 3723-3738
  • DOI: 10.1111/bph.14777
  • Peer Reviewed / Open Access
• [Journal Article] Protein adductomics: A comprehensive analysis of protein modifications by electrophiles (2019)
  • Author(s): Shibata Takahiro、Uchida Koji
  • Journal Title: Free Radical Biology and Medicine Volume: 144 Pages: 218-222
  • DOI: 10.1016/j.freeradbiomed.2019.02.034
  • Open Access
• [Journal Article] 2-Alkenal modification of hemoglobin: Identification of a novel hemoglobin-specific alkanoic acid-histidine adduct (2019)
  • Author(s): Yoshitake Jun、Shibata Takahiro、Shimayama Chihiro、Uchida Koji
  • Journal Title: Redox Biology Volume: 23 Pages: 101115-101115
  • DOI: 10.1016/j.redox.2019.101115
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial dynamics in exercise physiology. (2019)
  • Author(s): Tanaka T, Nishimura A, Nishiyama K, Goto T, Numaga-Tomita T, Nishida M.
  • Journal Title: Pflugers Archiv European Journal of Physiology Volume: 印刷中 Issue: 2 Pages: 1-17
  • DOI: 10.1007/s00424-019-02258-3
  • Peer Reviewed / Open Access
• [Journal Article] Current topics in prostaglandin D2 metabolites (2018)
  • Author(s): 柴田 貴広、内田 浩二
  • Journal Title: 生化学 Volume: 90 Issue: 5 Pages: 711-714
  • DOI: 10.14952/SEIKAGAKU.2018.900711
  • ISSN: 0037-1017
  • Year and Date: 2018-10-25
  • Open Access
• [Journal Article] 2-Oxo-histidine-containing dipeptides are functional oxidation products (2018)
  • Author(s): Ihara Hideshi、Kakihana Yuki、Yamakage Akane、Kai Kenji、Shibata Takahiro、Nishida Motohiro、Yamada Ken-ichi、Uchida Koji
  • Journal Title: Journal of Biological Chemistry Volume: 294 Issue: 4 Pages: 1279-1289
  • DOI: 10.1074/jbc.ra118.006111
  • Peer Reviewed / Open Access
• [Journal Article] Structural and functional insights into S-thiolation of human serum albumins. (2018)
  • Author(s): Nakashima F, Shibata T, Kamiya K, Yoshitake J, Kikuchi R, Matsushita T, Ishii I, Gimenez-Bastida JA, Schneider C, Uchida K
  • Journal Title: Sci Rep. Volume: 8(1) Issue: 1 Pages: 932-932
  • DOI: 10.1038/s41598-018-19610-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prolonged stimulation of β2-adrenergic receptor with β2-agonists impairs insulin actions in H9c2 cells (2018)
  • Author(s): Parichatikanond Warisara、Nishimura Akiyuki、Nishida Motohiro、Mangmool Supachoke
  • Journal Title: Journal of Pharmacological Sciences Volume: 138 Issue: 3 Pages: 184-191
  • DOI: 10.1016/j.jphs.2018.09.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission-associated myocardial senescence. (2018)
  • Author(s): Nishimura A, Shimauchi T, Tanaka T, Shimoda K, Toyama T, Kitajima N, Ishikawa T, Shindo N, Numaga-Tomita T, Yasuda S, Sato Y, Kuwahara K, Kumagai Y, Akaike T, Ide T, Ojida A, Mori Y, Nishida M.
  • Journal Title: Sci Signal Volume: 11 Issue: 556 Pages: 5185-5195
  • DOI: 10.1126/scisignal.aat5185
  • Peer Reviewed / Open Access
• [Journal Article] TRPC5-eNOS Axis Negatively Regulates ATP-Induced Cardiomyocyte Hypertrophy. (2018)
  • Author(s): Sunggip C, Shimoda K, Oda S, Tanaka T, Nishiyama K, Mangmool S, Nishimura A, Numaga-Tomita T and Nishida M
  • Journal Title: Front. Pharmacol. Volume: 9 Pages: 523-523
  • DOI: 10.3389/fphar.2018.00523
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] TRPC channels in exercise-mimetic therapy. (2018)
  • Author(s): Numaga-Tomita T, Oda S, Nishiyama K, Tanaka T, Nishimura A, Nishida M.
  • Journal Title: Pflugers Archiv European Journal of Physiology Volume: 471 Issue: 3 Pages: 507-517
  • DOI: 10.1007/s00424-018-2211-3
  • Peer Reviewed / Open Access
• [Journal Article] 心臓リモデリングを制御するGタンパク質／受容体シグナリング (2018)
  • Author(s): 西田基宏、西村明幸、西山和宏
  • Journal Title: 日本臨牀増刊「心不全（第2版） 上巻－最新の基礎・臨床研究の進歩－」 Volume: 76(9) Pages: 155-161
• [Journal Article] 8-Nitro-cGMP attenuates the interaction between SNARE complex and complexin through S-guanylation of SNAP-25 (2018)
  • Author(s): Kishimoto Y, Kunieda K, Kitamura A, Kakihana Y, Akaike T, Ihara H.
  • Journal Title: ACS Chem Neurosci Volume: 9 Issue: 2 Pages: 217-223
  • DOI: 10.1021/acschemneuro.7b00363
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of nitric oxide/reactive oxygen species signaling via 8-nitro-cGMP formation in 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in PC12 cells and rat cerebellar granule neurons (2018)
  • Author(s): Masuda Kumiko、Tsutsuki Hiroyasu、Kasamatsu Shingo、Ida Tomoaki、Takata Tsuyoshi、Sugiura Kikuya、Nishida Motohiro、Watanabe Yasuo、Sawa Tomohiro、Akaike Takaaki、Ihara Hideshi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 3 Pages: 2165-2170
  • DOI: 10.1016/j.bbrc.2017.12.088
  • Peer Reviewed / Open Access
• [Journal Article] Adductome-based identification of biomarkers for lipid peroxidation. (2017)
  • Author(s): Shibata T, Shimizu K, Hirano K, Nakashima F, Kikuchi R, Matsushita T, Uchida K.
  • Journal Title: J Biol Chem. Volume: 292(20) Issue: 20 Pages: 8223-8235
  • DOI: 10.1074/jbc.m116.762609
  • Peer Reviewed / Open Access
• [Journal Article] Roles of 5-lipoxygenase and cyclooxygenase-2 in the biosynthesis of hemiketals E2 and D2 by activated human leukocytes (2017)
  • Author(s): Gimenez-Bastida JA, Shibata T, Uchida K, Schneider C.
  • Journal Title: FASEB J. Volume: 31 Issue: 5 Pages: 1867-1878
  • DOI: 10.1096/fj.201601136r
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of polyphenol-specific innate epitopes originated from a resveratrol analogue. (2017)
  • Author(s): Furuhashi, M., Hatasa, Y., Kawamura, S., Shibata, T., Akagawa, M., Uchida, K.
  • Journal Title: Biochemistry Volume: 56 Issue: 35 Pages: 470-4712
  • DOI: 10.1021/acs.biochem.7b00409
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] TRPC3-Nox2 complex mediates doxorubicin-induced myocardial atrophy (2017)
  • Author(s): Shimauchi Tsukasa、Numaga-Tomita Takuro、Ito Tomoya、Nishimura Akiyuki、Matsukane Ryosuke、Oda Sayaka、Hoka Sumio、Ide Tomomi、Koitabashi Norimichi、Uchida Koji、Sumimoto Hideki、Mori Yasuo、Nishida Motohiro
  • Journal Title: The JCI insight Volume: 2 Issue: 15 Pages: 93358-93358
  • DOI: 10.1172/jci.insight.93358
  • Peer Reviewed / Open Access
• [Journal Article] TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice (2017)
  • Author(s): Oda Sayaka、Numaga-Tomita Takuro、Kitajima Naoyuki、Toyama Takashi、Harada Eri、Shimauchi Tsukasa、Nishimura Akiyuki、Ishikawa Tatsuya、Kumagai Yoshito、Birnbaumer Lutz、Nishida Motohiro
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 7511-7511
  • DOI: 10.1038/s41598-017-07903-4
  • NAID: 120007128849
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics (2017)
  • Author(s): Akaike Takaaki、、、Nishida Motohiro（4）、、、、、、Fujii Shigemoto（20）、、、Motohashi Hozumi（26）
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1177-1177
  • DOI: 10.1038/s41467-017-01311-y
  • NAID: 120007134656
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Exposure to Electrophiles Impairs Reactive Persulfide-Dependent Redox Signaling in Neuronal Cells (2017)
  • Author(s): Ihara Hideshi、Kasamatsu Shingo、Kitamura Atsushi、Nishimura Akira、Tsutsuki Hiroyasu、Ida Tomoaki、Ishizaki Kento、Toyama Takashi、Yoshida Eiko、Abdul Hamid Hisyam、Jung Minkyung、Matsunaga Tetsuro、Fujii Shigemoto、Sawa Tomohiro、Nishida Motohiro、Kumagai Yoshito、Akaike Takaaki
  • Journal Title: Chem. Res. Toxicol Volume: 30 Issue: 9 Pages: 1673-1684
  • DOI: 10.1021/acs.chemrestox.7b00120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] TRPC3 participates in angiotensin II type 1 receptor-dependent stress-induced slow increase in intracellular Ca2+ concentration in mouse cardiomyocytes (2017)
  • Author(s): Yamaguchi Y, Iribe G, Kaneko T, Takahashi K, Numaga-Tomita T, Nishida M, Birnbaumer L, Naruse K.
  • Journal Title: J. Physiol. Sci. Volume: 印刷中 Issue: 2 Pages: 1-12
  • DOI: 10.1007/s12576-016-0519-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MiR30-GALNT1/2 Axis-Mediated Glycosylation Contributes to the Increased Secretion of Inactive Human Prohormone for Brain Natriuretic Peptide (proBNP) From Failing Hearts. (2017)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Fujishima A, Oka S, Tsutamoto T, Kinoshita H, Nakao K, Cho K, Inazumi H, Okamoto H, Nishida M, Kato T, Fukushima H, Yamashita JK, Wijnen WJ, Creemers EE, Kangawa K, Minamino N, Nakao K, Kimura T.
  • Journal Title: J Am Heart Assoc. Volume: 6 Issue: 2
  • DOI: 10.1161/jaha.116.003601
  • NAID: 120007100125
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Redox regulation of electrophilic signaling by reactive persulfides in cardiac cells (2017)
  • Author(s): Nishida M, Nishimura A, Matsunaga T, Motohashi H, Kasamatsu S, Akaike T.
  • Journal Title: Free Radic Biol Med. Volume: 印刷中 Pages: 30033-3
  • DOI: 10.1016/j.freeradbiomed.2017.01.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] プリン作動性シグナルの心血管系における役割 (2017)
  • Author(s): 西村明幸、西田基宏
  • Journal Title: 日本薬理学雑誌 Volume: 149 Pages: 84-90
  • NAID: 130005306336
• [Journal Article] Identification of C1q as a binding protein for advanced glycation end products (2016)
  • Author(s): Chikazawa, M., Shibata, T., Hatasa, Y., Hirose, S., Otaki, N., Nakashima, F., Ito, M., Machida, S., Maruyama, S., Uchida, K.
  • Journal Title: Biochemistry Volume: 55 Issue: 3 Pages: 435-446
  • DOI: 10.1021/acs.biochem.5b00777
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oxidative deamination of serum albumins by (-)-epigallocatechin-3-O-gallate: A potential eechanism for the formation of innate antigens by antioxidants (2016)
  • Author(s): Hatasa, Y., Chikazawa, M., Furuhashi, M., Nakashima, F., Shibata, T., Kondo, T., Akagawa, M., Hamagami, H., Tanaka, H., Tachibana, H., Uchida, K.
  • Journal Title: PLoS One Volume: 11 Issue: 4 Pages: e0153002-e0153002
  • DOI: 10.1371/journal.pone.0153002
  • Peer Reviewed / Open Access
• [Journal Article] Identification of lactate dehydrogenase as a mammalian pyrroloquinoline quinone (PQQ)-binding protein (2016)
  • Author(s): Akagawa, M., Minematsu, K., Shibata, T., Kondo, T., Ishii, T., and Uchida, K.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 26723-26723
  • DOI: 10.1038/srep26723
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Membrane lipid peroxidation in neurodegeneration: Role of thrombin and proteinase-activated receptor-1 (2016)
  • Author(s): Citron, B. A., Ameenuddin, S., Uchida, K., Suo, W. Z., SantaCruz, K., and Festoff, B. W.
  • Journal Title: Brain Res. Volume: 1643 Pages: 10-17
  • DOI: 10.1016/j.brainres.2016.04.071
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis (2016)
  • Author(s): Nokin, M. J., Durieux, F., Peixoto, P., Chiavarina, B., Peulen, O., Blomme, A., Turtoi, A., Costanza, B., Smargiasso, N., Baiwir, D., De Tullio, P., Bianchi, E., Thiry, M., Uchida, K., Spiegel, D. A., Hutton, C. A., De Pauw, E., Delvenne, P., Belpomme, D., Castronovo, D., and Bellahcène, A.
  • Journal Title: eLife Volume: 5
  • DOI: 10.7554/elife.19375
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The purinergic P2Y6 receptor heterodimerizes with the angiotensin AT1 receptor to promote angiotensin II-induced hypertension (2016)
  • Author(s): A. Nishimura, C. Sunggip, H. Tozaki-Saitoh, T. Shimauchi, T. Numaga-Tomita, K. Hirano, T. Ide, J-M. Boeynaems, H. Kurose, M. Tsuda, B. Robaye, K. Inoue and M. Nishida
  • Journal Title: Science Signaling Volume: Jan 19;9(411) Issue: 411
  • DOI: 10.1126/scisignal.aac9187
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Screening of Transient Receptor Potential Canonical Channel Activators Identifies Novel Neurotrophic Piperazine Compounds (2016)
  • Author(s): S. Sawamura, M. Hatano, Y. Takada, K. Hino, T. Kawamura, J. Tanikawa, H. Nakagawa, H. Hase, A. Nakao, M. Hirano, R. Rotrattanadumrong, S. Kiyonaka, M.X. Mori, M. Nishida, Y. Hu, R. Inoue, R. Nagata and Y. Mori
  • Journal Title: Mol Pharmacol Volume: 89(3) Issue: 3 Pages: 348-363
  • DOI: 10.1124/mol.115.102863
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sustained betaAR stimulation mediates cardiac insulin resistance in a PKA-dependent manner (2016)
  • Author(s): S. Mangmool, T. Denkaew, S. Phosri, D. Pinthong, W. Parichatikanond, T. Shimauchi and M. Nishida
  • Journal Title: Mol. Endocrinol. Volume: 30(1) Issue: 1 Pages: 118-132
  • DOI: 10.1210/me.2015-1201
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Synthesis of radioiodinated probes to evaluate the biodistribution of a potent TRPC3 inhibitor (2016)
  • Author(s): M. Hagimori, T. Murakami, K. Shimizu, M. Nishida, T. Ohshima, T. Mukai
  • Journal Title: MedChemComm Volume: 7 Issue: 5 Pages: 1003-1006
  • DOI: 10.1039/c6md00023a
  • Peer Reviewed / Open Access
• [Journal Article] Methylmercury, an environmental electrophile capable of activation and disruption of the Akt/CREB/Bcl-2 signal transduction pathway in SH-SY5Y cells (2016)
  • Author(s): Unoki T, Abiko Y, Toyama T, Uehara T, Tsuboi K, Nishida M, Kaji T, Kumagai Y.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 28944-28944
  • DOI: 10.1038/srep28944
  • NAID: 120007135346
  • Peer Reviewed / Open Access
• [Journal Article] TRPC3 positively regulates reactive oxygen species driving maladaptive cardiac remodeling. (2016)
  • Author(s): Kitajima N., Numaga-Tomita T., Watanabe M., Kuroda T., Nishimura A., Miyano K., Yasuda S., Kuwahara K., Sato Y., Ide T., Birnbaumer L., Sumimoto H., Mori Y. and Nishida M
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 37701-37701
  • DOI: 10.1038/srep37001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis. (2016)
  • Author(s): Numaga-Tomita T., Kitajima N., Kuroda T., Nishimura A., Miyano K., Yasuda S., Kuwahara K., Sato Y., Ide T., Birnbaumer L., Sumimoto H., Mori Y. and Nishida M.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 39383-39383
  • DOI: 10.1038/srep39383
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Redox signaling regulated by electrophiles and reactive sulfur species (2016)
  • Author(s): Nishida M, Kumagai Y, Ihara H, Fujii S, Motohashi H, Akaike T.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 58 Issue: 2 Pages: 91-98
  • DOI: 10.3164/jcbn.15-111
  • NAID: 130005132519
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] アダクトーム解析法を用いた疾患バイオマーカーの探索 (2016)
  • Author(s): 柴田貴広、内田浩二
  • Journal Title: バイオサイエンスとインダストリー Volume: 74 Pages: 26-29
  • NAID: 40020705853
• [Journal Article] Redox signaling regulated by an electrophilic cyclic nucleotide and reactive cysteine persulfides (2016)
  • Author(s): Fujii S, Sawa T, Nishida M, Ihara H, Ida T, Motohashi H, Akaike T.
  • Journal Title: Arch Biochem Biophys. Volume: 595 Pages: 140-146
  • DOI: 10.1016/j.abb.2015.11.008
  • Peer Reviewed / Open Access
• [Journal Article] TRPC3 amplifies B cell receptor-induced ERK signaling via protein kinase D-dependent Rap1 activation (2016)
  • Author(s): T. Numaga-Tomita, M. Nishida, J.W. Jr. Putney and Y. Mori
  • Journal Title: Biochem J Volume: 473(2) Issue: 2 Pages: 201-210
  • DOI: 10.1042/bj20150596
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent progress in studies on the health benefits of pyrroloquinoline quinone (2015)
  • Author(s): Akagawa, M., Nakano, M., Ikemoto, K.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 80 Issue: 1 Pages: 13-22
  • DOI: 10.1080/09168451.2015.1062715
  • Peer Reviewed
• [Journal Article] Abnormal myofiber morphology and limb dysfunction in claudication. (2015)
  • Author(s): Koutakis, P., Myers, S. A., Cluff, K., Ha, D. M., Haynatzki, G., McComb, R. D., Uchida, K., Miserlis, D., Papoutsi, E., Johanning, J. M., Casale, G. P., and Pipinos, I. I.
  • Journal Title: J. Surg. Res. Volume: J. Surg. Res. Issue: 1 Pages: 172-179
  • DOI: 10.1016/j.jss.2015.02.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Generation of adducts of 4-hydroxy-2-nonenal with heat shock 60kDa protein 1 in human promyelocytic HL-60 and monocytic THP-1 cell lines. (2015)
  • Author(s): Arcaro, A., Daga, M., Paolo Cetrangolo, G., Ciamporcero, E., Lepore, A., Pizzimenti, A., Petrella, C., Graf, M., Uchida, U., Mamone, G., Ferranti, P., Julian Ames, R., Palumbo, G., BarreraG., and Gentile, F.
  • Journal Title: Oxid Med. Cell. Longev. Volume: 2015 Pages: 296146-296146
  • DOI: 10.1155/2015/296146
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Divergent Roles of CAAX Motif-signaled Posttranslational Modifications in the Regulation and Subcellular Localization of Ral GTPases (2015)
  • Author(s): LR Gentry, A. Nishimura, A.D. Cox, T.D. Martin, D. Tsygankov, M. Nishida, T.C. Elston and C.J. Der
  • Journal Title: J Biol Chem. Volume: 290(37) Issue: 37 Pages: 22851-22861
  • DOI: 10.1074/jbc.m115.656710
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reactive sulfur species-mediated activation of the Keap1-Nrf2 pathway by 1,2-napthoquinone through sulfenic acids formation under oxidative stress. (2015)
  • Author(s): Shinkai Y, Abiko Y, Ida T, Miura T, Kakehashi H, Ishii I, Nishida M, Sawa T, Akaike T, Kumagai Y.
  • Journal Title: Chem Res Toxicol. Volume: 印刷中 Issue: 5 Pages: 838-847
  • DOI: 10.1021/tx500416y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Measurement of Glucose Uptake in Cultured Cells (2015)
  • Author(s): Yamamoto N., Ueda-Wakagi M., Sato T., Kawasaki K., Sawada K., Kawabata K., Akagawa M., Ashida H.
  • Journal Title: Current Protocols in Pharmacology Volume: 12.14 Issue: 1 Pages: 1-26
  • DOI: 10.1002/0471141755.ph1214s71
  • Peer Reviewed
• [Journal Article] Production of hypoallergenic cocoa beans by a pregermination treatment method (2015)
  • Author(s): Handoyo, T., Akagawa, M., Kim, K.
  • Journal Title: J. Plant Biotechnol. Volume: 42 Issue: 2 Pages: 123-128
  • DOI: 10.5010/jpb.2015.42.2.123
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Aldehyde adducts generated during lipid peroxidation modification of proteins. (2015)
  • Author(s): Uchida K
  • Journal Title: Free Radic Res. Volume: 49 Issue: 7 Pages: 896-904
  • DOI: 10.3109/10715762.2015.1036052
  • Peer Reviewed
• [Journal Article] Special issue on "Recent progress in lipid peroxidation based on novel approaches (2015)
  • Author(s): Yin H, Niki E, Uchida K.
  • Journal Title: Free Radic Res. Volume: 49 Issue: 7 Pages: 813-815
  • DOI: 10.3109/10715762.2015.1021697
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 15-Deoxy-Δ12, 14-prostaglandin J2 as an electrophilic mediator (2015)
  • Author(s): Shibata T.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 79 Issue: 7 Pages: 1044-1049
  • DOI: 10.1080/09168451.2015.1012149
  • Open Access
• [Journal Article] 内因性抗原としての陰性荷電分子の生成と制御 (2015)
  • Author(s): 近澤未歩、内田浩二
  • Journal Title: 生化学 Volume: 87 Pages: 776-780
  • NAID: 40020687514
• [Journal Article] Palmitate induces insulin resistance in human HepG2 hepatocytes by enhancing ubiquitination and proteasomal degradation of key insulin signaling molecules (2015)
  • Author(s): Ishii M, Maeda A, Tani S, Akagawa M.
  • Journal Title: Arc.h Biochem. Biophys Volume: 566 Pages: 26-35
  • DOI: 10.1016/j.abb.2014.12.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Elastin modification by 4-hydroxynonenal in hairless mice exposed to UV-A. Role in photoaging and actinic elastosis. (2014)
  • Author(s): Larroque-Cardoso, P., Camaré, C., Nadal-WollboldF., Grazide, M. H., Pucelle, M., Garoby-Salom, S., Bogdanowicz, P., Josse, G., Schmitt, A. M., Uchida, K., Zarkovic, K., Salvayre, R., and Nègre-Salvayre, A.
  • Journal Title: J. Invest. Dermatol. Volume: 135 Issue: 7 Pages: 1873-1881
  • DOI: 10.1038/jid.2015.84
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Toll-like receptors as a target of food-derived anti-Inflammatory compounds. (2014)
  • Author(s): Shibata, T., Nakashima, F., Honda, K., Lu, Y. J., Kondo, T., Ushida, Y., Aizawa, K., Suganuma, H., Oe, S., Tanaka, H., Takahashi, T. and Uchida, K.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 47 Pages: 32757-32772
  • DOI: 10.1074/jbc.m114.585901
  • Peer Reviewed / Open Access
• [Journal Article] Functional interaction between cyclooxygenase-2 and p53 in response to an endogenous electrophile (2014)
  • Author(s): Kumagai, T., Usami, H., Matsukawa, N., Nakashima, F., Chikazawa, M., Shibata, T., Noguchi, N., and Uchida, K.
  • Journal Title: Redox Biol. Volume: 4 Pages: 74-86
  • DOI: 10.1016/j.redox.2014.11.011
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of N-type Ca2+ channels ameliorates an imbalance in cardiac autonomic nerve activity and prevents lethal arrhythmias in mice with heart failure. (2014)
  • Author(s): Yamada Y, Kinoshita H, Kuwahara K, Nakagawa Y, Kuwabara Y, Minami T, Yamada C, Shibata J, Nakao K, Cho K, Arai Y, Yasuno S, Nishikimi T, Ueshima K, Kamakura S, Nishida M, Kiyonaka S, Mori Y, Kimura T, Kangawa K, Nakao K.
  • Journal Title: Cardiovasc Research Volume: 104 Issue: 1 Pages: 183-193
  • DOI: 10.1093/cvr/cvu185
  • NAID: 120005619790
  • Peer Reviewed / Open Access
• [Presentation] DNA様構造特性をもつ修飾タンパク質の生成と病態 (2018)
  • Author(s): 内田浩二
  • Organizer: 「タンパク質のnon-canonical機能：機構と酸素生物学における意義」シンポジウム. 第91回日本生化学会大会
  • Invited
• [Presentation] 脂質酸化を起点とするタンパク質アダクトーム (2018)
  • Author(s): 柴田貴広、内田浩二
  • Organizer: 「脂質酸化を起点とするバイオロジーの新展開」シンポジウム. 第91回日本生化学会大会
  • Invited
• [Presentation] 酸化イミダゾールジペプチドの検出 (2018)
  • Author(s): 垣花優希，山陰茜，柴田貴広，内田浩二，居原秀
  • Organizer: レドックス研究会2018「生命のエネルギー獲得戦略における多様性と共通原
• [Presentation] 酸化イミダゾールジペプチド産生の分子メカニズムの解明 (2018)
  • Author(s): 垣花優希，内田浩二，居原秀
  • Organizer: 第18 回日本NO学会
• [Presentation] イミダゾールジペプチドによる抗酸化メカニズムの解明 (2018)
  • Author(s): 居原 秀
  • Organizer: 第16回レドックス・ライフイノベーションシンポジウム
  • Invited
• [Presentation] TRPC3 channels as a key regulator of cardiac plasticity (2018)
  • Author(s): Nishida M.
  • Organizer: The 18th World Congress of Basic and Clinical Pharmacology
  • Int'l Joint Research / Invited
• [Presentation] TRPC3/C6チャネルタンパク質による筋柔軟性制御 (2018)
  • Author(s): 西田基宏
  • Organizer: 第4回日本筋学会学術集会
  • Invited
• [Presentation] 酸素由来活性種によるタンパク質多量体化とその意義 (2018)
  • Author(s): 西田基宏
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] GPCR/Gタンパク質のレドックス修飾に着目した創薬 (2018)
  • Author(s): 西田基宏
  • Organizer: レドックス・ライフイノベーション第170委員会／日本におけるケミカルバイオロジーの新展開第189委員会合同シンポジウム
  • Invited
• [Presentation] Cardiovascular Aging Regulated by Heterodimerization of Angiotensin AT1 Receptor with Purinergic P2Y6 Receptor (2018)
  • Author(s): Nishida M.
  • Organizer: Angiotensin Gordon Research Conference 2018
  • Int'l Joint Research / Invited
• [Presentation] S-チオール化血清アルブミンによる炎症誘導機構の解明 (2018)
  • Author(s): 中島史恵、柴田貴広、菊地良介、松下正、内田浩二
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 自然抗体エピトープを有する食品成分の探索 (2018)
  • Author(s): 三輪恭行、今堀夏奈美、古橋麻衣、柴田貴広、内田浩二
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] ポリフェノールオキシダーゼによる茶カテキンの自然抗体リガンド生成能の増強 (2018)
  • Author(s): 今堀夏奈美、三輪恭行、李相美、柴田貴広、内田浩二
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 酸化ストレスに対する細胞内カルノシンの神経保護効果 (2017)
  • Author(s): 山陰茜、垣花優希、柴田貴広、内田浩二、居原秀
  • Organizer: 第17回日本NO学会学術集会
• [Presentation] 酸化ストレスに依存した酸化イミダゾールジペプチドの産生 (2017)
  • Author(s): 垣花優希、柴田貴広、内田浩二、居原秀
  • Organizer: 第70回日本酸化ストレス学会学術集会
• [Presentation] Detection and quantification of 2-oxo-histidine-containing dipeptides (2017)
  • Author(s): Ihara H, Kakihana Y, Shibata T, Uchida K
  • Organizer: The Society for Redox Biology and Medicine's (SfRBM) 24th Annual Meeting
  • Int'l Joint Research
• [Presentation] 脂質異常症に関連した修飾血清アルブミン構造の解析 (2017)
  • Author(s): 中島史恵、柴田貴広、菊地良介、松下正、内田浩二
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] Protein carbonyls as a marker of exposome (2017)
  • Author(s): Koji Uchida
  • Organizer: 第 90 回日本細菌学会総会. レドックス・環境応答-国際シンポジウム(新学術領域「酸素生物学」共催) “Stress responses in environmental and exposure biology”
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Int'l Joint Research / Invited
• [Presentation] Natural antibodies as a sensor of oxidation-specific epitopes (2016)
  • Author(s): Koji Uchida
  • Organizer: Shanghai Symposium on Lipid Metabolism and Related Diseases
  • Place of Presentation: Hope Hotel（Shanghai, China)
  • Year and Date: 2016-09-16
  • Int'l Joint Research / Invited
• [Presentation] Electrophiles as a multifunctional molecule (2016)
  • Author(s): Koji Uchida
  • Organizer: 第9回国際NO学会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Int'l Joint Research / Invited
• [Presentation] 親電子修飾によるタンパク質機能活性化 (2016)
  • Author(s): 内田浩二
  • Organizer: 第89回日本生化学会大会シンポジウム“オルガネラ環境を制御するレドックスシグナル”
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Invited
• [Presentation] リジンピロール化によるDNA様蛋白質の生成 (2015)
  • Author(s): 内田浩二
  • Organizer: 第15回日本蛋白質科学会年会ワークショップ「化学修飾の蛋白質科学と医療への展開」
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Invited
• [Presentation] 小分子酸素センサーによるタンパク質カルボニル化 (2015)
  • Author(s): 内田浩二
  • Organizer: 第15回日本蛋白質科学会年会ワークショップ「酸素リモデリング・レドックスシグナルとタンパク質修飾の新たな潮流」
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Invited
• [Presentation] ポリフェノールによる自然免疫抗原の生成 (2015)
  • Author(s): 内田浩二
  • Organizer: 第9回日本ポリフェノール学会年次大会シンポジウム「ポリフェノール研究の将来」
  • Place of Presentation: 奈良女子大学（奈良県奈良市）
  • Invited
• [Presentation] 化学的反応性からみる機能性食品成分の潜在能力 (2015)
  • Author(s): 内田浩二
  • Organizer: 第38回日本分子生物学会・第88回日本生学会合同大会ワークショップ「食品科学：食品の潜在能力を科学し、活用する」
  • Place of Presentation: 神戸ポートピアホテル（兵庫県神戸市）
  • Invited
• [Presentation] Pyrrolation Is a Naturally-occurring Covalent Protein Modification Involved in the Production of DNA Mimic Proteins (2014)
  • Author(s): Koji Uchida
  • Organizer: Asian Biological Medicine Summit Forum in Hangzhou
  • Place of Presentation: Hangzhou, China
  • Year and Date: 2014-11-22 – 2014-11-25
  • Invited
• [Presentation] Food-derived natural inhibitors of TLR signaling (2014)
  • Author(s): Koji Uchida
  • Organizer: HNE club/SAS meeting 2014 Lipids, Oxidation, Antioxidants and・・・・・
  • Place of Presentation: Toulouse, France
  • Year and Date: 2014-09-08 – 2014-09-10
  • Invited
• [Presentation] 生体防御反応に関わる機能性食品成分 (2014)
  • Author(s): 内田浩二
  • Organizer: 2014年度 日本生物工学会中部支部例会
  • Place of Presentation: 名古屋大学工学研究科ES総合館（愛知県名古屋市）
  • Year and Date: 2014-08-01
  • Invited
• [Book] レドックス疾患学 (2018)
  • Author(s): 中島史恵、柴田貴広、内田浩二
  • Total Pages: 276
  • Publisher: 羊土社
  • ISBN: 9784758103695
• [Book] Japanese Scientists in Science Signaling 2016「シグナリングに載った日本人研究者」 2017 Issue (2017)
  • Author(s): 西村明幸、西田基宏
  • Total Pages: 80
  • Publisher: Science Signaling AAAS, Cosmo Bio Co. Inc.
• [Book] Innovative Medicine (2015)
  • Author(s): Nishida M, Kuwahara K, Kozai D, Sakaguchi R, Mori Y.
  • Total Pages: 339
  • Publisher: Springer Japan
• [Book] 別冊医学のあゆみ「レドックスUPDATE：ストレス制御の臨床医学・健康科学」 (2015)
  • Author(s): 内田浩二
  • Total Pages: 360
  • Publisher: 医歯薬出版
• [Book] 細胞工学 Vol.34 No.4 (2015)
  • Author(s): 居原秀、大内雄也
  • Total Pages: 440
  • Publisher: 学研メディカル秀潤社
• [Book] 細胞工学 Vol.34 No.4 (2014)
  • Author(s): 熊谷嘉人、内田浩二
  • Total Pages: 440
  • Publisher: 学研メディカル秀潤社
• [Patent(Industrial Property Rights)] ベンゾイソオキサゾール化合物 (2018)
  • Inventor(s): 永田龍、森泰生、西田基宏、森誠之、冨田拓郎
  • Industrial Property Rights Holder: 永田龍、森泰生、西田基宏、森誠之、冨田拓郎
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-086929
  • Filing Date: 2018