|Budget Amount *help
¥190,190,000 (Direct Cost: ¥146,300,000、Indirect Cost: ¥43,890,000)
Fiscal Year 2018: ¥33,150,000 (Direct Cost: ¥25,500,000、Indirect Cost: ¥7,650,000)
Fiscal Year 2017: ¥33,150,000 (Direct Cost: ¥25,500,000、Indirect Cost: ¥7,650,000)
Fiscal Year 2016: ¥33,280,000 (Direct Cost: ¥25,600,000、Indirect Cost: ¥7,680,000)
Fiscal Year 2015: ¥33,150,000 (Direct Cost: ¥25,500,000、Indirect Cost: ¥7,650,000)
Fiscal Year 2014: ¥57,460,000 (Direct Cost: ¥44,200,000、Indirect Cost: ¥13,260,000)
|Outline of Final Research Achievements
Cells overexpressing active YAP undergo apical extrusion and are losers in competitions with normal cells. However, YAP overexpressing cells switched from losers to winners when cocultured with cells expressing Ras or Src. YAP overexpressing cells show decrease in cell-matrix adhesion, and cell adhesion mediated proliferation. However, they proliferate faster than control cells when co-cultured in dishes impeding cell adhesion. Thus, the role of YAP in cell competitions is context dependent in vitro.
In vivo, YAP activation in damaged hepatocytes promotes their elimination by in vivo liver mosaic analysis, and YAP activated epidermis cannot be engrafted onto donor mice and shrink away from control skin. In contrast, YAP activation in undamaged hepatocytes leads to proliferation. Thus, the role of YAP is context dependent also in vivo. Our results thus uncover the mechanism by which YAP influences cell competition, that is important for liver or skin grafting, or cancer progression.