| Budget Amount *help |
¥279,630,000 (Direct Cost: ¥215,100,000、Indirect Cost: ¥64,530,000)
Fiscal Year 2018: ¥51,220,000 (Direct Cost: ¥39,400,000、Indirect Cost: ¥11,820,000)
Fiscal Year 2017: ¥51,220,000 (Direct Cost: ¥39,400,000、Indirect Cost: ¥11,820,000)
Fiscal Year 2016: ¥51,350,000 (Direct Cost: ¥39,500,000、Indirect Cost: ¥11,850,000)
Fiscal Year 2015: ¥51,090,000 (Direct Cost: ¥39,300,000、Indirect Cost: ¥11,790,000)
Fiscal Year 2014: ¥74,750,000 (Direct Cost: ¥57,500,000、Indirect Cost: ¥17,250,000)
|
|---|
| Outline of Final Research Achievements |
How proteins are synthesized in the cell and then fold to a conformation are important problems. In this study, we have promoted folding studies that are coupled to translation, which have not previously been studied in detail, and also developed novel methods such as a cell-free translation system reconstituted from eukaryotic factors. We found that the translation elongation was frequently (~80% of tested genes) paused from the analysis for more than 1000 genes. We found novel noncanonical translation dynamics: intrinsic ribosome destabilization, which terminates the elongation in a stop codon independent manner. We also established a reconstructed cell-free translation system derived from human and Saccharomyces cerevisiae factors and investigated various translational dynamics.
|
