| Budget Amount *help |
¥109,200,000 (Direct Cost: ¥84,000,000、Indirect Cost: ¥25,200,000)
Fiscal Year 2018: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2017: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2016: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000)
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| Outline of Final Research Achievements |
Peroxisome homeostasis involving regulation of the number and metabolic functions is maintained by coordinating biogenesis, division, and degradation. Tail-anchored (TA) proteins localized by a single C-terminal transmembrane domain are found in all cellular membranes. Peroxisomal TAs were investigated in this research. A number of outcomes include 1) TA proteins to peroxisomes are targeted via the Pex19p- and Pex3p-dependent Class-I pathway. 2) VDAC2 was identified as restoring ZP114, a CHO cell mutant lacking peroxisome. We also discovered that the catalase released from peroxisomes via BAK pore eliminates H2O2, a toxic and major causative of the oxidative stress, in the cytosol for cell survival. 3) We discovered that the 17-kDa nucleoside diphosphate kinase-like protein, DYNAMO1, locally generates GTP in mitochondrial division and peroxisome-dividing machineries. These outstanding contributions to organelle homeostasis in molecular cell biology are highly appreciated.
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