タウのタンパク質老化と毒性機序

• Project Area: Prevention of brain protein aging and dementia
• Project/Area Number: 26117004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Gakushuin University (2016-2018); National Center for Geriatrics and Gerontology (2014-2015)
• Principal Investigator: 高島 明彦 学習院大学, 理学部, 教授 (00154774)
• Co-Investigator(Kenkyū-buntansha): 久永 眞市 首都大学東京, 理工学研究科, 教授 (20181092) ; 石垣 診祐 名古屋大学, 医学系研究科, 寄附講座助教 (40378170) ; 宮坂 知宏 同志社大学, 生命医科学部, 准教授 (90342857) ; 池川 雅哉 同志社大学, 生命医科学部, 教授 (60381943)
• Project Period (FY): 2014-07-10 – 2019-03-31
• Project Status: Granted (Fiscal Year 2018)
• Budget Amount: ¥237,250,000 (Direct Cost: ¥182,500,000、Indirect Cost: ¥54,750,000); Fiscal Year 2018: ¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000); Fiscal Year 2017: ¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000); Fiscal Year 2016: ¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000); Fiscal Year 2015: ¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000); Fiscal Year 2014: ¥57,200,000 (Direct Cost: ¥44,000,000、Indirect Cost: ¥13,200,000)
• Keywords: 神経科学 / 脳神経疾患 / 老化 / タウタンパク質 / Aβ / 認知症 / 脳機能 / 凝集 / リン酸化 / 神経細胞死 / タウ / 局在 / 過活動 / 樹状突起

Outline of Annual Research Achievements

計画研究班はアルツハイマー病研究の初期からタウタンパク質が認知症を引き起こす原因であると考えてこれまで研究を行い、タウを過剰にリン酸化する酵素の発見、タウ凝集機構を明らかにしてきた。認知症治療を考える上で加齢に伴う正常タンパクから病原タンパクへと転換するタンパク質老化機構を明らかにする目的で研究を行っている。
今年度、タウの軸索局在機構としてタウの軸索局在に関与するドメインとして PRR2 領域を同定した。生後１日目のマウス脳より微小管非結合画分を調製し、共免疫沈降法によりタウと結合しているタンパク質群の同定を行なった結果、タウ分子には複数のチューブリンおよび、タンパク質翻訳にかかわる多くの因子の結合が認められこれらがタウタンパク質の軸索局在に寄与することが示唆された。
タウタンパク質の新たな生理機能を探索する目的でタウ遺伝子ノックアウト(KO)マウスを調べたところ、タウKOマウスの摂食量増大が観察された。この原因をさらに検討した結果中脳腹側被蓋野における神経活動が亢進が観察され報酬系の異常に伴う摂食量の亢進に起因している可能性を示唆した。
脳発達期におけるタウのリン酸化とアイソフォームの変化についてマウス脳を用いて解析した。その結果、生後、高リン酸化されたタウは速やかに脱リン酸化され、0N3Rから0N4Rへ変化する。甲状腺ホルモン合成阻害剤（MMI）をマウスに与えたところ発達遅延に伴って脱リン酸化時期が遅延するが0N3Rから0N4Rへの変化時期は変わらなかったことから、タウのリン酸化とアイソフォーム変化は異なる制御を受けていることが示された。
タウ凝集について15種類の異なるFTDP-17変異を持つヒトリコンビナントタウの凝集を経時的に観察し、野生型タウと比較したところ顆粒状タウオリゴマーの増大が全ての変異体に共通する凝集体であることが明らかになった。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

これまで毎年、計画班内ミーティングを行い、互いの研究進捗、アイデアを共有し、タウタンパク質の生理機能、リン酸化から凝集に至る機構を明らかにするため共同研究を進めている。具体的には共通に必要な抗体作成、スクリーニングを行い、それらを共有して使用している。宮坂、石垣、高島、久永らはこれまでの成果を論文にまとめ報告し、残る新たなテーマに取り組んでいる。ここまでにタウの局在機構、異常局在機構について神経細胞の過興奮によってタウmRNAからタウ蛋白への翻訳促進が起こりタウのsomatodendriteへの異常局在が起こるという新たな知見を得ている。この異常局在からタウ過剰リン酸化、凝集について今年度検討を行ない、タウの局在変化から凝集に至る分子機構を解明するところまで漕ぎついている。さらに、タウの新たな生理機能が明らかになりつつあることから、当初目標に対して順調に進展していると考えている。

Strategy for Future Research Activity

・複合体の全容解明から生理的タウの存在様式を決定することにより、改めて毒性タウを定義する。さらに、タウオパチー神経の特徴である、タウの異常化と微小管の消失の関係について新たなモデルを構築し、解析する。
・ヒト脳及びアルツハイマー病患者脳を用いて、GSK3β活性とタウの異常リン酸化の関連を調べていく。アイソフォーム変化に関わるスプライシング機構を解析する。
・DREADDのシステムや電気生理学的な解析などを通じて、どのような報酬系の経路がタウの喪失に脆弱になって摂食異常に関与するのかを明らかにしていく。
・毒性タウ凝集体の決定、細胞死機構を決定し、樹状突起において観察されるタウの過剰発現から細胞死に至る過程を明らかにする。
・論文化を促進する

Research Products

• [Journal Article] Silencing of FUS in the common marmoset ( Callithrix jacchus ) brain via stereotaxic injection of an adeno-associated virus encoding shRNA (2018)
  • Author(s): Endo K, Ishigaki S, Masamizu Y, Fujioka Y, Watakabe A, Yamamori T, Hatanaka N, Nambu A, Okado H, Katsuno M, Watanabe H, Matsuzaki M, Sobue G
  • Journal Title: Neurosci Res. Volume: in press Pages: 30183-9
  • DOI: 10.1016/j.neures.2017.08.006
  • Peer Reviewed
• [Journal Article] Isoform-independent and -dependent phosphorylation of microtubule-associated protein tau in mouse brain during postnatal development. (2018)
  • Author(s): Tuerde, D., Kimura, T., Miyasaka, T., Furusawa, K., Shimozawa, A., Hasegawa, M., Ando, A., Hisanaga, S.
  • Journal Title: J Biol Chem Volume: 293 Pages: 1781-1793
  • DOI: 10.1074/jbc.m117.798918
  • Peer Reviewed / Open Access
• [Journal Article] Phospho-Tau Bar Code: Analysis of Phosphoisotypes of Tau and Its Application to Tauopathy. (2018)
  • Author(s): Kimura, T., Sharma, G., Ishiguro, K., Hisanaga, S.
  • Journal Title: Front Neurosci. Volume: 12 Pages: 44-44
  • DOI: 10.3389/fnins.2018.00044
  • Peer Reviewed / Open Access
• [Journal Article] Polyunsaturated fatty acid deficiency during neurodevelopment in mice models the prodromal state of schizophrenia through epigenetic changes in nuclear receptor genes. (2017)
  • Author(s): Maekawa M、Watanabe A、Iwayama Y、Kimura T、Hamazaki K、Balan S、Ohba H、Hisano Y、Nozaki Y、Ohnishi T、Toyoshima M、Shimamoto C、Iwamoto K、Bundo M、Osumi N、Takahashi E、Takashima A、Yoshikawa T
  • Journal Title: Transl Psychiatry Volume: 7
  • DOI: 10.1038/tp.2017.182
  • Peer Reviewed / Open Access
• [Journal Article] Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer's disease : analysis of Osaka mutation-knockin mice (2017)
  • Author(s): Umcda T, Kimura T, Yoshida K, Takao K, Fujita Y, Matsuyama S, Sakai A, Yamashita M, Yamashita Y, Ohnishi K, Suzuki M, Takuma H, Miyakawa T, Takashima A, Morita T, Mori H, Tomiyama T
  • Journal Title: Acta Neuropathol Commun Volume: 5 Pages: 59-59
  • DOI: 10.1186/s40478-017-0461-5
  • Peer Reviewed / Open Access
• [Journal Article] Local somatodendritic translation and hyperphosphorylation of tau protein triggered by AMPA and NMDA receptor stimulation. (2017)
  • Author(s): Shunsuke Kobayashi, Toru Tanaka, Yoshiyuki Soeda, Osborne F. X. Almeida, Akihiko Takashima
  • Journal Title: EBio Medicine Volume: 印刷中 Pages: 120-126
  • DOI: 10.1016/j.ebiom.2017.05.012
  • Peer Reviewed / Open Access
• [Journal Article] Iso-α-acids, Bitter Components of Beer, Prevent Inflammation and Cognitive Decline Induced in a Mouse Model of Alzheimer's Disease. (2017)
  • Author(s): Ano Y, Dohata A, Taniguchi Y, Hoshi A, Uchida K, Takashima A, Nakayama H.
  • Journal Title: J Biol Chem. Volume: 292 Pages: 3720-3728
  • DOI: 10.1074/jbc.m116.763813
  • Peer Reviewed
• [Journal Article] Altered tau isoform ratio caused by loss of FUS and SFPQ function leads to FTLD-like phenotypes (2017)
  • Author(s): Ishigaki S, Fujioka Y, Okada Y, Riku Y, Udagawa T, Honda D, Yokoi S, Endo K, Ikenaka K, Takagi S, Iguchi Y, Sahara N, Takashima A, Okano H, Yoshida M, Warita H, Aoki M, Watanabe H, Okado H, Katsuno H, Sobue G.
  • Journal Title: Cell Report Volume: 18 Pages: 1118-1131
  • DOI: 10.1016/j.celrep.2017.01.013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Treatment of intermittent hypoxia increases phosphorylated tau in the hippocampus via biological processes common to aging (2017)
  • Author(s): Yagishita S, Suzuki S, Yoshikawa K, Iida K, Hirata A, Suzuki M, Takashima A, Maruyama K, Hirasawa A, Awaji T.
  • Journal Title: Mol Brain. Volume: 10 Pages: 2-2
  • DOI: 10.1186/s13041-016-0282-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathologic Involvement of Glutamatergic Striatal Inputs From the Cortices in TAR DNA-Binding Protein 43?kDa-Related Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis (2017)
  • Author(s): Riku Y, Watanabe H, Yoshida M, Mimuro M, Iwasaki Y, Masuda M, Ishigaki S.
  • Journal Title: J Neuropathol Exp Neurol Volume: 76(9) Pages: 759-768
  • DOI: 10.1093/jnen/nlx055
  • Peer Reviewed
• [Journal Article] 3′UTR Length-Dependent Control of SynGAP Isoform α2 mRNA by FUS and ELAV-like Proteins Promotes Dendritic Spine Maturation and Cognitive Function (2017)
  • Author(s): Yokoi S, Udagawa T, Fujioka Y, Honda D, Okado H, Watanabe H, Katsuno M.
  • Journal Title: Cell Rep. Volume: 20(13) Pages: 3071-3084
  • DOI: 10.1016/j.celrep.2017.08.100
  • Peer Reviewed / Open Access
• [Journal Article] Altered GluA1 ( Gria1 ) Function and Accumbal Synaptic Plasticity in the Clock Δ19 Model of Bipolar Mania (2017)
  • Author(s): Parekh PK, Becker-Krail D, Sundaravelu P, Ishigaki S, Okado H, Sobue G, Huang Y, McClung CA.
  • Journal Title: Biol Psychiatry Volume: S0006 Pages: 31721-3
  • DOI: 10.1016/j.biopsych.2017.06.022
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct deposition of amyloid-β species in brains with Alzheimer's disease pathology visualized with MALDI imaging mass spectrometry (2017)
  • Author(s): Kakuda N, Miyasaka T, Iwasaki N, Nirasawa T, Wada-Kakuda S, Takahashi-Fujigasaki J, Murayama S, Ihara Y, Ikegawa M.
  • Journal Title: Acta Neuropathol Commun Volume: 5 Issue: 1 Pages: 73-73
  • DOI: 10.1186/s40478-017-0477-x
  • Peer Reviewed / Open Access
• [Journal Article] Ca2+/calmodulin-dependent protein kinase II promotes neurodegeneration caused by tau phosphorylated at Ser262/356 in a transgenic Drosophila model of tauopathy. (2017)
  • Author(s): Oka Mikiko、Fujisaki Naoki、Maruko-Otake Akiko、Ohtake Yosuke、Shimizu Sawako、Saito Taro、Hisanaga Shin-Ichi、Iijima Koichi M、Ando Kanae
  • Journal Title: J Biochem. Volume: 162 Pages: 335-342
  • DOI: 10.1093/jb/mvx038
  • Peer Reviewed
• [Journal Article] In vivo regulation of glycogen synthase kinase 3β activity in neurons and brains (2017)
  • Author(s): Krishnankutty, A., Kimura, T., Saito, T., Aoyagi, K., Asada, A., Takahashi, S., Ando, K., Ohara-Imaizumi, M., Ishiguro, K., Hisanaga, S.
  • Journal Title: Sci Rep. Volume: 7 Pages: 8602-8602
  • DOI: 10.1038/s41598-017-09239-5
  • Peer Reviewed / Open Access
• [Journal Article] Cdk5 Regulation of the GRAB-Mediated Rab8-Rab11 Cascade in Axon Outgrowth. (2017)
  • Author(s): Furusawa K, Asada A, Urrutia P, Gonzalez-Billault C, Fukuda M, Hisanaga SI.
  • Journal Title: J Neurosci. Volume: 37 Pages: 790-806
  • DOI: 10.1523/jneurosci.2197-16.2016
  • Peer Reviewed / Open Access
• [Journal Article] Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production (2017)
  • Author(s): Akasaka-Manya K, Kawamura M, Tsumoto H, Saito Y, Kitazume S, Hatsuta H, Miura Y, Hisanaga S, Murayama S, Hashimoto Y, Manya H, Endo, T.
  • Journal Title: J Biochem. Volume: 161 Issue: 1 Pages: 99-111
  • DOI: 10.1093/jb/mvw056
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 複雑怪奇なリン酸化タンパク質タウのPhos-tag解析 (2017)
  • Author(s): 木村妙子、久永眞市
  • Journal Title: 電気泳動 Volume: 61 Pages: 49-52
  • Peer Reviewed
• [Journal Article] Cdk5の多彩な機能-神経細胞から非神経系 (2017)
  • Author(s): 斎藤太郎、久永眞市
  • Journal Title: 生化学 Volume: 89 Pages: 710-718
  • Peer Reviewed
• [Journal Article] Modification of Tau by 8-nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP): Effects of nitric oxide-linked chemical modification on Tau aggregation (2016)
  • Author(s): Yoshitake J, Soeda Y, Ida T, Sumioka A, Yoshikawa M, Matsushita K, Akaike T, Takashima A.
  • Journal Title: Journal of Biological Chemistry Volume: 291 Pages: 22714-22720
  • DOI: 10.1074/jbc.m116.734350
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mechanism of neurodegeneration through tau and therapy for Alzheimer disease. (2016)
  • Author(s): Takashima A
  • Journal Title: Journal of Sport and Health Science Volume: 5 Pages: 391-392
  • DOI: 10.1016/j.jshs.2016.08.009
  • Peer Reviewed / Open Access
• [Journal Article] Tau phosphorylation at Alzheimer's disease-related Ser356 contributes to tau stabilization when PAR-1/MARK activity is elevated (2016)
  • Author(s): Ando K, Oka M, Ohtake M, Hayashishita M, Shimizu S, Hisanaga S, Iijima KM
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 478 (2) Pages: 929-34
  • DOI: 10.1016/j.bbrc.2016.08.053
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Quantitative and combinatory determination of in situ phosphorylation of tau and its FTDP-17 mutants (2016)
  • Author(s): Kimura T, Hosokawa T, Taoka M, Tsutsumi K, Ando K, Ishiguro K, Hosokawa M, Hasegawa M, Hisanaga S.
  • Journal Title: Scientific Reports Volume: 6 Pages: 33479-33479
  • DOI: 10.1038/srep33479
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The kinase activity of endosomal kinase LMTK1A regulates its cellular localization and interactions with cytoskeletons (2016)
  • Author(s): Sharma S, Tsutsumi K, Saito T, Asada A, Ando K, Tomomura T, Hisanaga S.
  • Journal Title: Genes Cells. Volume: 21 Pages: 1080-1094
  • DOI: 10.1111/gtc.12404
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Effect of Fragmented Pathogenic α-Synuclein Seeds on Prion-like Propagation. (2016)
  • Author(s): Tarutani A, Suzuki G, Shimozawa A, Nonaka T, Akiyama H, Hisanaga S, and Hasegawa M.
  • Journal Title: J Biol Chem. Volume: 291 Pages: 18675-18688
  • DOI: 10.1074/jbc.m116.734707
  • Peer Reviewed
• [Journal Article] α-Synuclein fibrils exhibit gain-of-toxic-function, promoting tau aggregation and inhibiting microtubule assembly. (2016)
  • Author(s): Oikawa T, Nonaka T, Hisanaga S, Hasegawa M.
  • Journal Title: J Biol Chem. Volume: 291 Pages: 15046-15056
  • DOI: 10.1074/jbc.m116.736355
  • Peer Reviewed
• [Journal Article] Templated Aggregation of TAR DNA-binding Protein of 43 kDa (TDP-43) by Seeding with TDP-43 Peptide Fibrils. (2016)
  • Author(s): Nonaka T, Suzuki G, Tanaka Y, Kametani F, Hirai S, Okado H, Miyashita T, Saitoe M, Akiyama H, Masai H, Hasegawa M.
  • Journal Title: J Biol Chem. Volume: 291 Pages: 8896-907
  • DOI: 10.1074/jbc.m115.713552
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Two degradation pathways of the p35 Cdk5 activation subunit, dependent and independent of ubiquitination. (2016)
  • Author(s): Takasugi et al.,
  • Journal Title: J. Biol. Chem. Volume: 291 Pages: 4649-4657
  • DOI: 10.1074/jbc.m115.692871
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Abundance of Nonphosphorylated Tau in Mouse and Human Tauopathy Brains Revealed by the Use of Phos-Tag Method. (2016)
  • Author(s): Kimura T, Hatsuta H, Masuda-Suzukake M, Hosokawa M, Ishiguro K, Akiyama H, Murayama S, Hasegawa M, Hisanaga S.
  • Journal Title: Am J Pathol. Volume: 186 Pages: 398-409
  • DOI: 10.1016/j.ajpath.2015.10.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gene Expression Profile of Inflammatory Myopathy with Malignancy is Similar to that of Dermatomyositis rather than Polymyositis (2016)
  • Author(s): Noda, T., Iijima, M., Noda, S., Maeshima, S., Nakanishi, H., Kimura, S., Koike, H., Ishigaki, S., Iguchi, Y., Katsuno, M., and Sobue, G.
  • Journal Title: Intern Med. Volume: 55 Issue: 18 Pages: 2571-2580
  • DOI: 10.2169/internalmedicine.55.6706
  • Peer Reviewed
• [Journal Article] Involvement of the caudate nucleus head and its networks in sporadic amyotrophic lateral sclerosis-frontotemporal dementia continuum. (2016)
  • Author(s): Masuda M, Senda J, Watanabe H, Epifanio B, Tanaka Y, Imai K, Riku Y, Li Y, Nakamura R, Ito M, Ishigaki S, Atsuta N, Koike H, Katsuno M, Hattori N, Naganawa S, and Sobue G.
  • Journal Title: Amyotroph Lateral Scler Frontotemporal Degener Volume: 17 Pages: 571-579
  • Peer Reviewed
• [Journal Article] Marked Involvement of the Striatal Efferent System in TAR DNA-Binding Protein 43kDa-Related Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis. (2016)
  • Author(s): Riku Y, Watanabe H, Yoshida M, Mimuro M, Iwasaki Y, Masuda M, Ishigaki S, Katsuno M, Sobue G.
  • Journal Title: J Neuropathol Exp Neurol Volume: 印刷中
  • Peer Reviewed
• [Journal Article] R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular (2016)
  • Author(s): Nakashima H., Ohkawara B., Ishigaki S., Fukudome T., Ito K., Tsushima M., Konishi H., Okuno T., Yoshimura T., Ito M., Masuda A., Sobue G., Kiyama H., Ishiguro N., Ohno K.
  • Journal Title: Sci Rep. Volume: 22 Pages: 28512-28512
  • DOI: 10.1038/srep28512
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A rapid functional decline type of amyotrophic lateral sclerosis is linked to low (2016)
  • Author(s): Watanabe H, Atsuta N, Hirakawa A, Nakamura R, Nakatochi M, Ishigaki S, Iida A, Sobue G.
  • Journal Title: Journal of Neurology, Neurosurgery & Psychiatry Volume: in press Pages: 851-8
  • DOI: 10.1136/jnnp-2015-311541
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of the carboxyl-terminal sequence of tau and MAP2 in the pathogenesis of dementia. (2016)
  • Author(s): Xie C, Miyasaka T.
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 9 Pages: 158-158
  • DOI: 10.3389/fnmol.2016.00158
  • Peer Reviewed / Open Access
• [Journal Article] Drug discovery focused on the pathological mechanisms of tauopathy. (2016)
  • Author(s): Miyasaka T.
  • Journal Title: Proceeding of the Annual Meeting of the Japanese Research Group on Senile Dementia Volume: 21 Pages: 1-5
  • Open Access
• [Journal Article] Imaging Mass Spectrometry for the Research of Alzheimer’s Disease (2016)
  • Author(s): Ikegawa M, Miyasaka T, Kakuda N, Ihara Y
  • Journal Title: J Mass Spectrom. Soc. Jpn Volume: 64 Issue: 1 Pages: 17-20
  • DOI: 10.5702/massspec.S16-04
  • Peer Reviewed / Open Access
• [Journal Article] The Advantages of Structural Equation Modeling to Address the Complexity of Spatial Reference Learning. (2016)
  • Author(s): Moreira PS, Sotiropoulos I, Silva J, Takashima A, Sousa N, Leite-Almeida H, Costa PS.
  • Journal Title: Front Behav Neurosci. Volume: 10 Pages: 18-18
  • DOI: 10.3389/fnbeh.2016.00018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] I2020T mutant LRRK2 iPSC-derived neurons in the Sagamihara family exhibit increased Tau phosphorylation through the AKT/GSK-3β signaling pathway. (2015)
  • Author(s): Ohta E, Nihira T, Uchino A, Imaizumi Y, Okada Y, Akamatsu W, Takahashi K, Hayakawa H, Nagai M, Ohyama M, Ryo M, Ogino M, Murayama S, Takashima A, Nishiyama K, Mizuno Y, Mochizuki H, Obata F, Okano H
  • Journal Title: Hum Mol Genet. Volume: 24(17) Pages: 4879-4900
  • DOI: 10.1093/hmg/ddv212
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of key amino acids responsible for the distinct aggregation properties of microtubule-associated protein 2 and tau. (2015)
  • Author(s): Xie C, Soeda Y, Shinzaki Y, In Y, Tomoo K, Ihara Y, Miyasaka T.
  • Journal Title: J Neurochem Volume: 135 Pages: 19-26
  • DOI: 10.1111/jnc.13228
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Glycogen Synthase Kinase-3β-mediated Phosphorylation in the Most C-terminal Region of Protein Interacting with C Kinase 1 (PICK1) Regulates the Binding of PICK1 to Glutamate Receptor Subunit GluA2. (2015)
  • Author(s): Yagishita S, Murayama M, Ebihara T, Maruyama K, Takashima A
  • Journal Title: J Biol Chem Volume: 290 Pages: 29438-48
  • DOI: 10.1074/jbc.m114.619668
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Toxic tau oligomer formation blocked by capping of cysteine residue with 1,2-dihydroxybenzene groups (2015)
  • Author(s): Soeda Y, Yoshikawa M, Almeida OF, Sumioka A, Maeda S, Osada H, Kondoh Y, Saito A, Miyasaka T, Kimura T, Suzuki M, Koyama H, Yoshiike Y, Sugimoto H, Ihara Y, Takashima A
  • Journal Title: Nature Commun. Volume: 6 Pages: 10216-10216
  • DOI: 10.1038/ncomms10216
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] FUS regulates AMPA receptor function and FTLD/ALS-associated behavior via GluA1 mRNA stabilization. (2015)
  • Author(s): Udagawa, T., Fujioka, Y., Tanaka, M., Honda, D., Yokoi, S., Riku, Y., Ibi, D., Nagai, T., Yamada, K., Watanabe, H., Katsuno, M., Inada, T., Ohno, K., Sokabe, M., Okado, H., Ishigaki S*., and Sobue, G.
  • Journal Title: Nat Commun. Volume: 6 Pages: 7098-7098
  • DOI: 10.1038/ncomms8098
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptional and post-transcriptional regulation of transmembrane protein 132A. (2015)
  • Author(s): Oh-Hashi, K., Sone, A., Hikiji, T., Hirata, Y., Vitiello, M., Fedele, M., Ishigaki S., Sobue, G., and Kiuchi, K.
  • Journal Title: Mol Cell Biochem Volume: 405 Pages: 291-299
  • DOI: 10.1007/s11010-015-2419-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Position-specific binding of FUS to nascent RNA regulates mRNA length (2015)
  • Author(s): Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, Ishigaki S, Sobue G, Ohno K.
  • Journal Title: Genes Dev Volume: 29(10) Pages: 10451057-10451057
  • DOI: 10.1101/gad.255737.114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A highly sensitive assay of IRE1 activity using the small luciferase NanoLuc: Evaluation of ALS-related genetic and pathological factors. (2015)
  • Author(s): Hikiji, T., Norisada, J., Hirata, Y., Okuda, K., Nagasawa, H., Ishigaki S., Sobue, G., Kiuchi, K., and Oh-hashi, K.
  • Journal Title: Biochem Biophys Res Commun Volume: 463 Pages: 881-887
  • DOI: 10.1016/j.bbrc.2015.05.132
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Microglial Keratan Sulfate Epitope Elicits in Central Nervous Tissues of Transgenic Model Mice and Patients with Amyotrophic Lateral Sclerosis. (2015)
  • Author(s): Foyez T, Takeda-Uchimura Y, Ishigaki S, Narentuya, Zhang Z, Sobue G, Kadomatsu K, Uchimura K.
  • Journal Title: Am J Pathol. Volume: 185 Pages: 3053-3065
  • DOI: 10.1016/j.ajpath.2015.07.016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of p35 mutations associated with mental retardation on the cellular function of p35-CDK5 (2015)
  • Author(s): Takada S, Mizuno K, Saito T, Asada A, Giese KP, Hisanaga S.
  • Journal Title: PLOSONE Volume: 10
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Extracellular association of APP and tau fibrils induces intracellular aggregate formation of tau (2015)
  • Author(s): Takahashi M, Miyata H, Kametani F, Nonaka T, Akiyama H, Hisanaga SI, Hasegawa M.
  • Journal Title: Acta Neuropathol Volume: 129 Pages: 893-907
  • DOI: 10.1007/s00401-015-1415-2
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Female Hippocampus Vulnerability to Environmental Stress as Precipitating Factor in Tau Aggregation Pathology. (2015)
  • Author(s): Sotiropoulos I, Silva J, Kimura T, Rodrigues AJ, Costa P, Almeida OF, Sousa N, Takashima A.
  • Journal Title: J Alzheimers Dis. Volume: 43 Pages: 763-774
  • DOI: 10.3233/jad-140693
  • Peer Reviewed
• [Journal Article] Extracellular association of APP and tau fibrils induces intracellular aggregate formation of tau. (2015)
  • Author(s): Takahashi, M., Miyata, H., Kametani, F., Nonaka, T., Akiyama, H., Hisanaga, S., Hasegawa, M.
  • Journal Title: Acta Neuropathol. Volume: in press
  • Peer Reviewed
• [Journal Article] How Does the Ca²⁺-paradox Injury Induce Contracture in the Heart?—A Combined Study of the Intracellular Ca²⁺ Dynamics and Cell Structures in Perfused Rat Hearts— (2015)
  • Author(s): Mani H, Tanaka H, Adachi T, Ikegawa M, Dai P, Fujita N, Takamatsu T.
  • Journal Title: Acta Histochem Cytochem Volume: 48 Issue: 1 Pages: 1-8
  • DOI: 10.1267/ahc.14059
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptional and post-transcriptional regulation of transmembrane protein 132A. (2015)
  • Author(s): Oh-Hashi K, Sone A, Hikiji T, Hirata Y, Vitiello M, Fedele M, Ishigaki S, Sobue G, Kiuchi K.
  • Journal Title: Mol Cell Biochem. Volume: in press
  • Peer Reviewed
• [Journal Article] Position-specific binding of FUS to nascent RNA regulates mRNA length. (2015)
  • Author(s): Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, Ishigaki S, Sobue G, Ohno K.
  • Journal Title: Gen. Dev. Volume: in press
  • Peer Reviewed
• [Journal Article] FUS regulates AMPA receptor function and FTLD/ALS-associated behavior via GluA1 mRNA stabilization. (2015)
  • Author(s): Udagawa T, Fujioka Y, Tanaka M, Honda D, Yokoi S, Riku Y, Ibi D, Nagai T, Yamada K, Watanabe H, Katsuno M, Inada T, Ohno K, Sokabe M, Okado H, Ishigaki S*, Sobue G*.
  • Journal Title: Nat Commun. Volume: in press
  • Peer Reviewed / Open Access
• [Journal Article] Neurofibrillary tangle formation by introducing wild-type human tau into APP transgenic mice (2014)
  • Author(s): Umeda T, Maekawa S, Kimura T, Takashima A, Tomiyama T, Mori H.
  • Journal Title: Acta Neuropathol Volume: 127(5) Pages: 685-698
  • DOI: 10.1007/s00401-014-1259-1
  • Peer Reviewed
• [Journal Article] Biochemical distribution of tau protein in synaptosomal fraction of transgenic mice expressing human P301L tau. (2014)
  • Author(s): Sahara N, Murayama M, Higuchi M, Suhara T, Takashima A.
  • Journal Title: Front Neurol. Volume: 5 Pages: 26-26
  • DOI: 10.3389/fneur.2014.00026
  • Peer Reviewed / Open Access
• [Journal Article] Development of disease modifying drugs for dementia—focusing on anti-tau drugs— (2014)
  • Author(s): 添田義行, 高島明彦
  • Journal Title: 臨床神経学 Volume: 54 Issue: 12 Pages: 1178-1180
  • DOI: 10.5692/clinicalneurol.54.1178
• [Journal Article] サイクリン依存性キナーゼ５の活性制御機構と神経細胞死における役割 (2014)
  • Author(s): 斎藤太郎、久永眞市
  • Journal Title: 生体の科学 Volume: 65 Pages: 266-270
• [Journal Article] LMTK1 regulates dendritic formation by regulating movement of Rab11A-positive endosomes. (2014)
  • Author(s): Takano et al
  • Journal Title: Mol Biol. Cell Volume: 25 Pages: 1755-1768
  • DOI: 10.1091/mbc.e14-01-0675
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Valproic acid downregulates Cdk5 activity via transcription of p35 mRNA. (2014)
  • Author(s): Takahashi, et al.
  • Journal Title: Biochem. Biophysic Res Commun. Volume: 447 Pages: 678-682
  • DOI: 10.1016/j.bbrc.2014.04.072
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The effect of Cyclin-dependent kinase 5 on voltage-dependent calcium channels in PC12 cells varies according to channel type and cell differentiation state. (2014)
  • Author(s): Furusawa et al.,
  • Journal Title: J. Neurochem. Volume: 130 Pages: 498-506
  • DOI: 10.1111/jnc.12746
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ,Phosphorylation of Cdk5 at Tyr15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5 (2014)
  • Author(s): Ito, Y., Asada, A., Kobayashi, H., Takano, T., Sharma, G., Saito, T., Ohta, Y., Amano, M., Kaibuchi, K., and Hisanaga, S.
  • Journal Title: Mol Cell Neurosci. Volume: 61 Pages: 19627-19636
  • DOI: 10.1016/j.mcn.2014.05.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phosphorylation of Cdk5 at Tyr15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5. (2014)
  • Author(s): Kobayashi, H., Saito, T., Sato, K., Furusawa, K., Hosokawa, T., Tsutsumi, K., Asada, A., Kamada, S., Ohshima, T., and Hisanaga, S.
  • Journal Title: J. Biol. Chem. Volume: 289 Pages: 19627-19636
  • DOI: 10.1074/jbc.m113.501148
  • Peer Reviewed
• [Journal Article] Physiological and pathological phosphorylation of tau by Cdk5. (2014)
  • Author(s): Kimura, T., Ishiguro, K., Hisanaga, S.
  • Journal Title: Frontiers Mol. Neurosci. Volume: 7 Pages: 65-65
  • DOI: 10.3389/fnmol.2014.00065
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Filamin acts as a key regulator in epithelial defence against transformed cells (2014)
  • Author(s): Kajita M., Sugimura, K., Ohoka, A., Burden, J., Suganuma, H., Ikegawa, M., Shimada, T., Kitamura, T., Shindoh, M., Ishikawa, S., Yamamoto, S., Saitoh, S., Yako, Y., Takahashi, R., Okajima, T., Kikuta, J., Maijima, Y., Ishii, M., Tada, M., and Fujita, Y.
  • Journal Title: Nat Commun. Volume: 5 Pages: 4428-4428
  • DOI: 10.1038/ncomms5428
  • Peer Reviewed / Open Access
• [Presentation] タウタンパク質老化機構 (2017)
  • Author(s): 高島明彦
  • Organizer: 第30回日本老年学会総会シンポジウム
• [Presentation] Local somatodendrititc translation of tau protein triggered by AMPA and NMDA receptor stimulation (2017)
  • Author(s): 高島明彦
  • Organizer: Society for Neuroscience 2017
  • Int'l Joint Research
• [Presentation] タンパク質の生理機能と認知症における役割 (2017)
  • Author(s): 高島明彦
  • Organizer: CnBio(分子生物学会）ワークショップ
• [Presentation] ALS/FTLDにおけるFUSの機能の重要性 (2017)
  • Author(s): 石垣診祐
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] 3’UTR length-dependent control of SynGAP isoform α2 mRNA by FUS and ELAV-like proteins promotes dendritic spine maturation and cognitive function. (ポスター） (2017)
  • Author(s): Yokoi S, Udagawa T, Fujioka Y, Honda D, Okado H, Watanabe H, Katsuno M, Ishigaki S, Sobue G.
  • Organizer: WCN 2017
  • Int'l Joint Research
• [Presentation] Silencing of FUS in the brain of non-human primates via stereotaxic injection of an adeno-associated virus encoding shRNA.(ポスター） (2017)
  • Author(s): Endo K, Ishigaki S, Fujioka Y, Watanabe H, Katsuno M, Sobue G.
  • Organizer: WCN 2017
  • Int'l Joint Research
• [Presentation] Aberrant interaction between FUS and SFPQ in 4R-tau dominant tauopathy brains. (ポスター） (2017)
  • Author(s): Ishigaki S, Riku Y, Fujioka Y, Honda D, Yokoi S, Endo K, Kawai K, Watanabe H, Katsuno M, Sobue G.
  • Organizer: The 47th Society for Neuroscience annual meeting
  • Int'l Joint Research
• [Presentation] Silencing of FUS in the non-human primate brain via stereotaxic injection of an adeno-associated virus encoding shRNA. (ポスター） (2017)
  • Author(s): Endo K, Ishigaki S, Fujioka Y, Watanabe H, Katsuno M, Sobue G.
  • Organizer: The 47th Society for Neuroscience annual meeting
  • Int'l Joint Research
• [Presentation] FUS and ELAV-like proteins cooperatively control SynGAP isoform α2 in a 3'UTR length-dependent manner to promote dendritic spine maturation and cognitive function. (2017)
  • Author(s): Yokoi S, Udagawa T, Fujioka Y, Honda D, Okado H, Watanabe H, Katsuno M, Ishigaki S, Sobue G.
  • Organizer: The 47th Society for Neuroscience annual meeting
  • Int'l Joint Research
• [Presentation] Development of a single cell analysis tool for neurological disorders on 2D-plate with positional information. （ポスター） (2017)
  • Author(s): Ishigaki S, Uno H, Wang Z-H, Ukita Y, Bhardwaj R, Tue PT, Yurino H, Hashimoto S, Oka T, Kawahara K, Urisu T, Takamura Y, and Sobue G.
  • Organizer: The 40th annual Meeting of the Japan Neuroscience Society
• [Presentation] Cooperative control of SynGAP isoform α2 mRNA stability by FUS and ELAV-like proteins promotes dendritic spine maturation and cognitive function. (ポスター） (2017)
  • Author(s): Yokoi S, Udagawa T, Fujioka Y, Honda D, Okado H, Watanabe H, Katsuno M, Ishigaki S, Sobue G.
  • Organizer: The 40th annual Meeting of the Japan Neuroscience Society
• [Presentation] Establishment of adeno-associated virus vector system for silencing FUS in common marmoset brain. (ポスター） (2017)
  • Author(s): Endo K, Ishigaki S, Masamizu Y, Hatanaka N, Fujioka Y, Watakabe A, Yamamori T, Katsuno M, Watanabe H, Matsuzaki M, Nambu A, Sobue G.
  • Organizer: The 40th annual Meeting of the Japan Neuroscience Society
• [Presentation] Ubiquitin-dependent and -independent degradation of p35 activation subunit of neuronal Cdk, Cdk5. (2017)
  • Author(s): Takasugi T, Minegishi S, Asada A, Saito T, Hiroyuki K, Hisanaga S.
  • Organizer: Experimental Biology 2017 (Annual meeting of American Socity of Biochemistry and Molecullar Biology)
  • Int'l Joint Research
• [Presentation] エンドソームのトラッフィキング制御と軸索形成及び維持 (2017)
  • Author(s): 久永眞市
  • Organizer: SAM研究会シンポジウム
• [Presentation] Cdk5 regulation of the GRAB-mediated Rab11-Rab8 cascade in axon outgrowth. (2017)
  • Author(s): Furusawa K, Asada A, Urrutia P, Gonzalez-Billault C, Fukuda M, Hisanaga S.
  • Organizer: 第60回日本神経化学会
• [Presentation] LMTK1 is a novel membrane bound kinase involved in anxiety and depression. (2017)
  • Author(s): Takahashi M, Sugiyama A, Takahashi R, Fukuda K, Tomomura M, Ando K, Hisanaga S.
  • Organizer: ISN-ESN Meeting 2017
  • Int'l Joint Research
• [Presentation] Dendritic spine formation is regulated by Lemur kinase 1A (LMTK1A) via Rab11A-positive endosome trafficking. (2017)
  • Author(s): Nishino H, Takano T, Tsutsumi K, Asada A, Saito T, Ando K, Hisanaga S.
  • Organizer: ISN-ESN Meeting 2017
  • Int'l Joint Research
• [Presentation] Isoform-independent and -dependent phosphorylation of microtubule-associated protein tau in mouse brain during postnatal development. (2017)
  • Author(s): Tuerde D, Kimura T, Miyasaka T, Furusawa K, Shimozawa A, Hasegawa M, Ando K, Hisanaga S.
  • Organizer: The International Society of Neurochemistry (ISN) and the European Society for Neurochemistry (ESN)
  • Int'l Joint Research
• [Presentation] Phosphorylation of serotonin 1A receptor (5HT1AR) by Cdk5. (2017)
  • Author(s): Takahashi M, Kobayashi Y, Ando K, Saito Y, Hisanaga S.
  • Organizer: 第60回日本神経化学会大会
• [Presentation] Cyclin-dependent kinase 5, its role in neuronal differentiation, synaptic activity and neurodegeneration. (2017)
  • Author(s): Hisanaga S.
  • Organizer: Royan International Conference
  • Int'l Joint Research
• [Presentation] 複雑怪奇なリン酸化タンパク質タウのPhos-tag解析 (2017)
  • Author(s): 木村妙子、久永眞市
  • Organizer: 電気泳動学会シンポジウム
• [Presentation] Isoform-independent and -dependent phosphorylation of microtubule-associated protein tau in mouse brain during postnatal development. (2017)
  • Author(s): Tuerde D, Kimura T, Miyasaka T, Furusawa K, Shimozawa A, Hasegawa M, Ando K, Hisanaga S.
  • Organizer: Cell symposium: Big question in Neuroscience
  • Int'l Joint Research
• [Presentation] Cdk5-dependent phosphorylation of GRAB, a guanine nucleotide exchange factor for Rab8, regulates neuronal migration in the developing cerebral cortex. (2017)
  • Author(s): Furusawa K, Asada A, Urrutia P, Gonzalez-Billault C, Fukuda M, Hisanaga S.
  • Organizer: ASCB/EMBO 2017 Meeting
  • Int'l Joint Research
• [Presentation] アルツハイマー型認知症関連因子βセクレターゼ（BACE1）の細胞内輸送におけるLemur kinase1A（LMTK1A）の役割 (2017)
  • Author(s): 駒木圭介、池田識人、佐藤裕、淺田明子、斉藤太郎、久永眞市
  • Organizer: 第40回日本分子生物学会
• [Presentation] Phospho-tau bar code－死後脳でのタウリン酸化解析はタウオパチーの診断に役立つか？ (2017)
  • Author(s): 久永眞市
  • Organizer: 新学術領域研究「コホート・生体試料支援プラットフォーム」研究者対象シンポジウム
• [Presentation] 認知症治療最前線 (2017)
  • Author(s): 高島明彦
  • Organizer: 学際生命科学東京コンソーシアム 第11回市民講演会
  • Place of Presentation: 東京医科歯科大学
  • Invited
• [Presentation] Unravelling Alzheimer’s Brain Pathology with MALDI Imaging mass spectrometry (2017)
  • Author(s): 池川雅哉
  • Organizer: 第１回京都生体質量分析研究会シンポジウム
  • Place of Presentation: 京都
  • Invited
• [Presentation] Unraveling amyloid beta-pathology in Alzheimer's Disease with MALDI Imaging Mass Spectrometry (2017)
  • Author(s): 池川雅哉
  • Organizer: 17th Korean Human Proteome Organization
  • Place of Presentation: Seoul
  • Invited
• [Presentation] Mechanism of neurodegeneration and therapy for Alzheimer's disease (2016)
  • Author(s): 高島明彦
  • Organizer: AD International Conference in Hefei
  • Place of Presentation: University of Science and Technology of China
  • Invited
• [Presentation] タウとアルツハイマー病 (2016)
  • Author(s): 高島明彦
  • Organizer: 埼玉医科大学 卒後教育委員会 後援 学術集会
  • Place of Presentation: 埼玉医科大学
  • Invited
• [Presentation] 認知症治療最前線 (2016)
  • Author(s): 高島明彦
  • Organizer: 第88回学習院大学技術交流会
  • Place of Presentation: 学習院大学
  • Invited
• [Presentation] タウのタンパク質老化と毒性機序 (2016)
  • Author(s): 高島明彦
  • Organizer: 次世代脳プロジェクト 冬のシンポジウム
  • Place of Presentation: 一橋大学
  • Invited
• [Presentation] Functional linkage between FUS and tau in ALS/FTLD (2016)
  • Author(s): Ishigaki S, Fujioka Y, Honda D, Yokoi S, Okado H, Watanabe H, Katsuno M, Sobue G.
  • Organizer: The 39th annual Meeting of the Japan Neuroscience Society Symposium
  • Place of Presentation: Yokohama, Japan
• [Presentation] Accumulation of phosphorylated tau in FUS-silenced mice (2016)
  • Author(s): Ishigaki S
  • Organizer: Brain Protein Aging and Dementia Control International Workshop 2016
  • Place of Presentation: Nagoya（ポスター）
• [Presentation] 神経細胞におけるアルツハイマー病原因蛋白質タウの軸索局在の分子機序 (2016)
  • Author(s): 岩田美里、渡邊祥司、宮坂知宏、朴洪宣、御園生裕明
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 東北大学（仙台）
• [Presentation] 脳発生に伴うタウ関連タンパク質の発現変化 (2016)
  • Author(s): 上田翔洋、角田聡子、久保厚子、宮坂知宏
  • Organizer: 第35回日本認知症学会学術集会
  • Place of Presentation: 東京国際フォーラム（東京）
• [Presentation] 神経細胞におけるタウ蛋白質の軸索局在化機構の解明 (2016)
  • Author(s): 岩田美里、渡邊祥司、宮坂知宏、朴洪宣、御園生裕明
  • Organizer: 第35回日本認知症学会学術集会
  • Place of Presentation: 東京国際フォーラム（東京）
• [Presentation] Cdk5-dependent phosphorylation of GRAB, a guanine nucleotide exchange factor for Rab8, regulates axon outgrowth by directing Rab8A to Rab11A-positive endosomes. (2016)
  • Author(s): Furusawa K, Asada A, Urrutia P, Gonzalez-Billault C, Fukuda M, Hisanaga S.
  • Organizer: American Society for Cell Biology
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2016-12-03
• [Presentation] イメージング質量分析法を用いたアルツハイマー病脳タンパク質の解析 (2016)
  • Author(s): 池川雅哉
  • Organizer: 第35回日本認知症学会学術集会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2016-12-01
• [Presentation] βセクレターゼ（BACE1）の細胞内輸送におけるLemur kinase1（LMTK1）の役割 (2016)
  • Author(s): 駒木圭介、池田識人、高野哲也、友村美根子、淺田明子、斎藤太郎、安藤香奈絵、久永眞市
  • Organizer: 第３９回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] DEVELOPMENT OF A MOLECULAR ANALYSIS CHIP FOR SINGLE CELLS ON 2D-PLANE WITH POSITIONAL INFORMATION (2016)
  • Author(s): Takamura Y, Uno H, Wang Z-H, Ishigaki S, Shimura R, Tue PT, Miyuki C, Shimoda T, Ukita Y, Urisu T, Kawahara K
  • Organizer: International conference on single cell research 2016
  • Place of Presentation: Tokyo（ポスター）
  • Year and Date: 2016-11-16
• [Presentation] In vivo regulation of GSK3b activity unveiled by the quantitative measurement of its phospho-isotypes. (2016)
  • Author(s): Krishnankutty K, Kimura T, Saito T, Aoyagi K, Asada A, Ando K, Ohara-Imaizumi M, Ishiguro K, Hisanaga S.
  • Organizer: 46th annual meeting of Society for Neuroscience
  • Place of Presentation: サンディエゴ、アメリカ
  • Year and Date: 2016-11-12
• [Presentation] Accumulation of phosphorylated tau in neurons in FUS-silenced mice (2016)
  • Author(s): Ishigaki S, Fujioka Y, Honda D, Yokoi S, Endo K., Okado H, Watanabe H, Katsuno M, Takashima A., Sobue G.
  • Organizer: The 46th Society for Neuroscience annual meeting
  • Place of Presentation: San Diego, CA
  • Year and Date: 2016-11-12
• [Presentation] Loss of SFPQ, an intra-nuclear counterpart of FUS causes FTLD-like phenotypes (2016)
  • Author(s): Fujioka Y, Ishigaki S, Yokoi S, Honda D, Okado H, Watanabe H, Katsuno M, Sobue G.
  • Organizer: The 46th Society for Neuroscience annual meeting
  • Place of Presentation: San Diego, CA（ポスター）
  • Year and Date: 2016-11-12
• [Presentation] プレーナパッチクランプによる単一細胞解析と外部汚染の検証 (2016)
  • Author(s): 宇野 秀隆, 王 志宏, 石垣 診祐, 浮田 昭, 高村 禅, 宇理須 恒雄
  • Organizer: 第77用物理学会秋季学術講演会
  • Place of Presentation: 新潟（ポスター）
  • Year and Date: 2016-09-13
• [Presentation] Phosphorylation and Isoform Expression of Microtubule-Associated Protein Tau are Regulated Independently during Brain Development. (2016)
  • Author(s): Tuerde D, Kimura T, Miyasaka T, Asada A, Saito1 T, Ando, K, Hisanaga S.
  • Organizer: Asian-Pacific Society for Neurochemistry
  • Place of Presentation: クアラルンプール、マレーシア
  • Year and Date: 2016-08-26
• [Presentation] Loss of PSF/SFPQ, an intra-nuclear counterpart of FUS causes FTLD-like phenotypes (2016)
  • Author(s): Fujioka Y, Ishigaki S, Yokoi S, Honda D, Okado H, Watanabe H, Katsuno M, Sobue G.
  • Organizer: The 39th annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜（ポスター）
  • Year and Date: 2016-07-20
• [Presentation] 樹状突起スパイン形成におけるLemur kinase1A(LMTK1A) の役割 (2016)
  • Author(s): 西野尋紀、堤弘次、浅田明子、斎藤太郎、久永眞市
  • Organizer: 第68回日本細胞生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2016-06-15
• [Presentation] Loss of FUS causes phosphorylated tau accumulation in aged mice (2016)
  • Author(s): 石垣 診祐、藤岡 祐介、本田 大祐、横井 聡、岡戸 晴生、渡辺 宏久、勝野 雅央、祖父江 元
  • Organizer: 第57回神経学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2016-05-18
• [Presentation] Loss of PSF/SFPQ, an intra-nuclear counterpart of FUS causes FTLD-like phenotypes (2016)
  • Author(s): 藤岡 祐介、石垣 診祐、本田 大祐、横井 聡、岡戸 晴生、渡辺 宏久、勝野 雅央、祖父江 元
  • Organizer: 第57回神経学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2016-05-18
• [Presentation] 前頭側頭葉変性症と筋萎縮性側索硬化症の線条体ではstriosomeの障害が特徴である (2016)
  • Author(s): 陸 雄一、渡辺 宏久、吉田 眞里、三室 マヤ、岩崎 靖、石垣 診祐、祖父江 元、勝野 雅央
  • Organizer: 第57回神経学会学術大会
  • Place of Presentation: 神戸（ポスター）
  • Year and Date: 2016-05-18
• [Presentation] A rapid functional decline type of sporadic ALS is linked to low expression of TTN (2016)
  • Author(s): Naoki Atsuta, Hazuki Watanabe, AkihiroHirakawa, Ryoichi Nakamura, Masahiro Nakatochi, Shinsuke Ishigaki, Aritoshi Iida, Shiro Ikegawa, Daichi Yokoi, Masahisa Katsuno, Yuishin Izumi, Mitsuya Morita, Akira Taniguchi, Koji Abe, Kouichi Mizoguchi, Osamu Kano, Ryuji Kaji, Gen Sobue,
  • Organizer: 第57回神経学会学術大会
  • Place of Presentation: 神戸（ポスター）
  • Year and Date: 2016-05-18
• [Presentation] Mechanism of Neurodegeneration through Tau and Therapy for AD. (2016)
  • Author(s): Takashima A
  • Organizer: The 1st International Gachon Neuroscience Symposium,
  • Place of Presentation: Korea
  • Year and Date: 2016-02-24
  • Int'l Joint Research / Invited
• [Presentation] Strategy of tau related therapy. (2015)
  • Author(s): 高島明彦
  • Organizer: 第55回日本核医学会学術総会 シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-11-06
  • Invited
• [Presentation] タウイメージングに期待されること (2015)
  • Author(s): 高島明彦
  • Organizer: 第34回日本認知症学会学術集会
  • Place of Presentation: 青森
  • Year and Date: 2015-10-02
  • Invited
• [Presentation] アルツハイマー病治療に向けて：タウ研究からのアプローチ (2015)
  • Author(s): 高島明彦
  • Organizer: 第10回青森神経科学談話会
  • Place of Presentation: 弘前
  • Year and Date: 2015-07-28
  • Invited
• [Presentation] イメージング質量分析を用いたアルツハイマー病脳の解析 (2015)
  • Author(s): 池川雅哉
  • Organizer: 日本質量分析学会 第63回質量分析総合討論会
  • Place of Presentation: つくば市
  • Year and Date: 2015-06-18
• [Presentation] Proteomic Imaging of amyloids in Brains from Amyloid Precursor Protein (APP) Transgenic Mice in comparison with Human Alzheimer Disease. (2015)
  • Author(s): Masaya Ikegawa, Tomohiro Miyasaka, Noriyuki Iwasaki, Takashi Nirasawa, Hiroyuki Sumikura, Shigeo Murayama, Yasuo Ihara.
  • Organizer: 63rd ASMS Conference on Mass Spectrometry and Allied Topics.
  • Place of Presentation: St. Louis, USA
  • Year and Date: 2015-05-31 – 2015-06-04
• [Presentation] Proteomic Mass Imaging of Pancreas from Type 2 Diabetes (T2D) Rat Model (2015)
  • Author(s): Noriyuki Iwasaki, Kei Masuyama, Hiroshi Wakazono, Takashi Nirasawa, Hirofumi Fujigaya, Masumi Higashiyama, Daisuke Hibi, Mayu Shukutani, Yuki Kuzuhara, Hiroyuki Yanagi, Masaya Ikegawa
  • Organizer: 63rd ASMS Conference on Mass Spectrometry and Allied Topics.
  • Place of Presentation: St. Louis, USA
  • Year and Date: 2015-05-31 – 2015-06-04
• [Presentation] Therapy against toxic tau aggregation in AD (2015)
  • Author(s): 高島明彦
  • Organizer: 第56回日本神経学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-05-21
  • Invited
• [Presentation] Distribution of endogenous and exogenous tau in the mouse brain (2015)
  • Author(s): Atsuko Kubo, Hiroaki Misonou, Makoto Matsuyama, Akihiko Takashima, Yasuo Ihara, Tomohiro Miyasaka
  • Organizer: The 12th International Conference on Alzheimer's and Parkinson's Diseases
  • Place of Presentation: Nice, France
  • Year and Date: 2015-03-21
• [Presentation] Aging and Tau (2015)
  • Author(s): Takashima A.
  • Organizer: Asian Aging Core for Longevity (AACL) 10 years and Beyond Japan-Korea Joint Seminars
  • Place of Presentation: Osaka, Japan.
  • Year and Date: 2015-03-12
  • Invited
• [Presentation] タウの基礎研究から試みる認知症治療薬開発 (2015)
  • Author(s): 高島明彦
  • Organizer: シンポジウム「アルツハイマー病先制治療薬の創出」
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-01-17
• [Presentation] 脳特異的キナーゼCdk5の活性制御と生理および病理 (2014)
  • Author(s): 久永眞市
  • Organizer: 第４回メトロポリタン脳の老化・認知症ファーラム 特別講演
  • Place of Presentation: 東京
  • Year and Date: 2014-12-04
  • Invited
• [Presentation] tau-Tg マウス脳における内在性および外来性タウの神経細胞内局在 (2014)
  • Author(s): 久保厚子、御園生裕明、松山誠、高島明彦、井原康夫、宮坂知宏
  • Organizer: 第33回日本認知症学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-12-01
• [Presentation] FUS/TLSからみたALS/FTLDのRNA病態 (2014)
  • Author(s): 石垣診祐、祖父江 元
  • Organizer: 第33回 日本認知症学会学術集会 シンポジウム
  • Place of Presentation: 横浜市
  • Year and Date: 2014-12-01
  • Invited
• [Presentation] 正常脳におけるタウの神経細胞内局在 (2014)
  • Author(s): 久保厚子、御園生裕明、松田響子、松山誠、河田光博、初田裕幸、村山繁雄、井原康夫、宮坂知宏
  • Organizer: 第33回日本認知症学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-30
• [Presentation] FUSとSFPQの複合体はMaptの選択的splicingを制御しALS/FTLDの高次機能障 害に関与する (2014)
  • Author(s): 石垣 診祐、 藤岡 祐介、 宇田川 剛、本田 大祐、横井 聡、渡辺 宏 久、勝野 雅央、祖父江 元
  • Organizer: 第33回 日本認知症学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-29 – 2014-12-01
• [Presentation] FUSはGluA1 mRNA安定性の調節を介してシナプス機能及びFTLD様行動を制御する (2014)
  • Author(s): 渡辺宏久、宇田川剛、藤岡祐介、本田大祐、横井聡、石垣診祐、田中基樹、曽我 部正博、永井拓、山田清文、岡戸晴生、大野欽司、勝野雅央、祖父江元
  • Organizer: 第33回 日本認知症学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-29 – 2014-12-01
• [Presentation] Rab結合タンパク質GRABのCdk5-p35によるリン酸化とその役割 (2014)
  • Author(s): 古澤孝太郎、浅田明子、斎藤太郎、福田光則、久永眞市
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-27
• [Presentation] 新規の神経セリン/スレオニンキナーゼ LMTK1 の新生ニューロンの移動や形態における役割 (2014)
  • Author(s): 漆原智己、高橋路佳、網本紫、高野哲也、土屋幸憲、斎藤太郎、友村美根子、久永眞市.
  • Organizer: 第37回日本分子生物学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-27
• [Presentation] アミロイド前駆体タンパクAPPの細胞内輸送に対するCD2APおよびCIN85の役割 (2014)
  • Author(s): 金子秀一郎、浅田明子、斎藤太郎、久永真市
  • Organizer: 第37回日本分子生物学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-27
• [Presentation] The involvement of glial cells in FUS-related ALS/FTLD pathophysiology -FUS knock-down glial cells have a neuro-protective effect. (2014)
  • Author(s): Yusuke Fujioka, Shinsuke Ishigaki, Takashi Shiromizu, Tsuyoshi Udagawa,Satoshi Yokoi, Daiyu Honda, Kensuke Ikenaka, Masahisa Katsuno, Takeshi Tomonaga and Gen Sobue
  • Organizer: The 43rd Society for Neuroscience annual meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] PP1-binding neuronal protein kinase LMTK1 suppresses neurite outgrowth through the regulation of Rab11-dependent endosomal trafficking. (2014)
  • Author(s): Takano, T., Tsutsumi, K., Fukuda, M., Tomomura, M., Hisanaga, S.
  • Organizer: 第11回国際フォスファターゼコンファレンス
  • Place of Presentation: 仙台市
  • Year and Date: 2014-11-13
  • Invited
• [Presentation] 脳神経疾患解析のための時間・空間プロテオミックイメージング (2014)
  • Author(s): 池川雅哉、韮澤 崇、岩崎了教
  • Organizer: 日本質量分析学会関東談話会イメージングマスワークショップ
  • Place of Presentation: 東京都
  • Year and Date: 2014-11-10
  • Invited
• [Presentation] Cdk5活性化因子p35のプロテアソーム分解について (2014)
  • Author(s): 高杉俊之、嶺岸正治、小林弘侑、斎藤太郎、浅田明子、久永眞市.
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2014-10-18
• [Presentation] タウタンパク質老化と毒性機序 (2014)
  • Author(s): 高島明彦
  • Organizer: 脳タンパク質老化と認知症制御キックオフシンポジウム
  • Place of Presentation: 東京都千代田区
  • Year and Date: 2014-10-08
• [Presentation] p39で活性化されるCdk5のRac1依存的ラメリポディアへの選択的集積 (2014)
  • Author(s): 淺田明子、伊藤有紀, 高野哲也, Sharma Govinda, 斉藤太郎, 太田安隆、天野睦紀, 貝淵弘三, 久永眞市.
  • Organizer: 第57回日本神経化学会大会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-09-30
• [Presentation] 電位依存性カルシウムチャネル活性に対するCdk5の影響 (2014)
  • Author(s): 古澤孝太郎、浅田明子、斎藤太郎、久永眞市
  • Organizer: 第57回日本神経化学会大会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-09-30
• [Presentation] New insights in tau phosphorylation obtained by Phos-tag SDS-PAGE analysis. (2014)
  • Author(s): Kimura, T., Hatsuta, H., Masuda-Suzukake, M., Ishiguro, K., Akiyama, H., Murayama, S., Hasegawa, M., Hisanaga, S.
  • Organizer: 第57回日本神経化学会大会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-09-29
• [Presentation] 微小管結合タンパク質タウのGSK3βによる生理的および病理的リン酸化のPhos-tag法を用いた再検証 (2014)
  • Author(s): 米澤遼、 網本紫、木村妙子、斎藤太郎、 久永眞市
  • Organizer: 第57回日本神経化学会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-09-29
• [Presentation] Tau /tau interaction through Cys residue form a toxic tau aggregate. (2014)
  • Author(s): Takashima A.
  • Organizer: 7th Asian Aging Core for Longevity (AACL) conference in Jeju
  • Place of Presentation: Jeju, Korea.
  • Year and Date: 2014-09-22
  • Invited
• [Presentation] マウス脳における内在性および外来性 tau の局在 (2014)
  • Author(s): 久保厚子、御園生裕明、松山誠、高島明彦、井原康夫、宮坂知宏
  • Organizer: 第37回日本神経科学大会 Neuroscience 2014
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-12
• [Presentation] Quality loss of FUS and SFPQ in the nucleus causes FTLD-like behavioral abnormalities by modulating Tau isoforms. (2014)
  • Author(s): Shinsuke Ishigaki, Yusuke Fujioka, Tsuyoshi Udagawa, Daiyu Honda, Satoshi Yokoi, Masahisa Katsuno, Haruo Okado, and Gen Sobue
  • Organizer: The 37th annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] The involvement of glial cells in FUS-related ALS/FTLD pathology -FUS knock-down glial cells have a neurotrophic effect.- (2014)
  • Author(s): Yusuke Fujioka, Shinsuke Ishigaki, Takashi Shiromizu, Tsuyoshi Udagawa, Satoshi Yokoi, Daiyu Honda, Kensuke Ikenaka, Masahisa Katsuno, Takeshi Tomonaga, and Gen Sobue
  • Organizer: The 37th annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 神経脱髄疾患の時間・空間的多発性の解析 (2014)
  • Author(s): 池川雅哉
  • Organizer: 第67回日本酸化ストレス学会
  • Place of Presentation: 京都市
  • Year and Date: 2014-09-05
  • Invited
• [Presentation] Characterizing Mouse Thymus using Imaging Mass Microscope. (2014)
  • Author(s): Yudai Tsuji, Hayato Nishitani, Tomoyuki Nakamura, Yumi Matsumoto, Hideshi Fujiwake, Kei Tashiro, Masaya Ikegawa.
  • Organizer: International Mass Spectrometry Conference,
  • Place of Presentation: Geneva, Switzerland.
  • Year and Date: 2014-08-29
• [Presentation] Plasma proteomic biomarker strategy in a porcine hepatectomy model. (2014)
  • Author(s): Kohta Iguchi, Masaya Ikegawa, Etsuro Hatano, Takashi Nirasawa, Noriyuki Iwasaki, Tomohito Sakai, Kenya Yamanaka, Motohiko Sato, Gen Yamamoto, Tatsuya Okamoto, Yosuke Kasai, Kojiro Taura, Kei Tashiro, Shinji Uemoto.
  • Organizer: International Mass Spectrometry Conference,
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2014-08-29
• [Presentation] The effect of Cyclin-dependent kinase 5 on voltage-dependent calcium channels in PC12 cells varies according to channel type and cell differentiation state. (2014)
  • Author(s): Furusawa, K., Asada, A., Saito, T., Hisanaga, S.
  • Organizer: 第12回アジア太平洋神経化学会
  • Place of Presentation: 台湾
  • Year and Date: 2014-08-25
• [Presentation] Phosphorylation of Drebrin by Cyclin-dependent kinase 5 and Its Role in Neuronal Migration. (2014)
  • Author(s): Tanabe, K., Yamazaki, H., Inaguma, Y., Asada, A., Kimura, T., Takahashi, J., Taoka, M., Ohshima, T., Furuichi, T., Isobe, T., Nagata, K., Shirao, T., Hisanaga, S.
  • Organizer: 第12回アジア太平洋神経化学会
  • Place of Presentation: 台湾
  • Year and Date: 2014-08-25
• [Presentation] New aspects of tau phosphorylation obtained by Phos-tag SDS-PAGE. (2014)
  • Author(s): Kimura, T., Hosokawa, T., Tsutsumi, K., Masuda-Suzukake, M., Hosokawa, M., Ishiguro, K., Akiyama, H., Murayama, S., Hasegawa, M., Hisanaga, S.
  • Organizer: 第12回アジア太平洋神経化学会
  • Place of Presentation: 台湾
  • Year and Date: 2014-08-24
• [Presentation] Effect of valproic acid on Cdk5 activity and mouse behavior. (2014)
  • Author(s): Takahashi, M., Ishida, M., Saito, T., Ohshima, T., Hisanaga, S.
  • Organizer: 第12回アジア太平洋神経化学会
  • Place of Presentation: 台湾
  • Year and Date: 2014-08-24
• [Presentation] タウ分子からアプローチするアルツハイマー病 (2014)
  • Author(s): 高島明彦
  • Organizer: 公益財団法人新世代研究所 バイオ単分子研究会
  • Place of Presentation: 静岡県伊東市
  • Year and Date: 2014-07-26
  • Invited
• [Presentation] Granular tau oligomer as toxic tau aggregation form in AD. (2014)
  • Author(s): Takashima A.
  • Organizer: Alzheimer’s Association International Conference 2014
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2014-07-14
• [Patent(Industrial Property Rights)] TDP-43の発現量を調節するアンチセンスオリゴヌクレオチド及びその用途 (2017)
  • Inventor(s): 祖父江、石垣、佐橋、永井、前田、武内、閨、藤原、松田
  • Industrial Property Rights Holder: 国立大学法人 名古屋大学/大阪大学, (株)神戸天然物化学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Application Year: 2017
• [Patent(Industrial Property Rights)] 細胞内容物の回収方法および回収装置 (2017)
  • Inventor(s): 宇理須恒夫、石垣診祐、宇野秀隆、高村禅
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Application Year: 2017
• [Patent(Industrial Property Rights)] 2,4-ジアミノフェノール誘導体、及び、タウ及び／又はアミロイドβの凝集阻害剤 (2017)
  • Inventor(s): 宮坂知宏、杉本八郎、井上善一 他4名
  • Industrial Property Rights Holder: 同志社大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017-03-08
  • Application Year: 2017
  • Overseas
• [Patent(Industrial Property Rights)] 灌流用注射針 (2016)
  • Inventor(s): 宮坂知宏、久保厚子
  • Industrial Property Rights Holder: 同志社大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-08-22
  • Application Year: 2016
  • Overseas
• [Patent(Industrial Property Rights)] イメージング質量分析の前処理方法 (2016)
  • Inventor(s): 池川雅哉、宮坂知宏、角田伸人、韮沢崇
  • Industrial Property Rights Holder: 同志社大学、ブルカー・ダルトニクス株式会社
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-231372
  • Filing Date: 2016-11-29
  • Application Year: 2016