| Budget Amount *help |
¥225,290,000 (Direct Cost: ¥173,300,000、Indirect Cost: ¥51,990,000)
Fiscal Year 2018: ¥37,960,000 (Direct Cost: ¥29,200,000、Indirect Cost: ¥8,760,000)
Fiscal Year 2017: ¥37,960,000 (Direct Cost: ¥29,200,000、Indirect Cost: ¥8,760,000)
Fiscal Year 2016: ¥52,260,000 (Direct Cost: ¥40,200,000、Indirect Cost: ¥12,060,000)
Fiscal Year 2015: ¥80,470,000 (Direct Cost: ¥61,900,000、Indirect Cost: ¥18,570,000)
Fiscal Year 2014: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
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| Outline of Final Research Achievements |
We developed a fusion protein method to create crystal contact-free space (CCFS) in protein crystals and to place the mobile parts or ligands in the CCFS. The mobile parts/ligands appear as smeared electron densities in the CCFS in the difference electron density map. We applied the CCFS method to visualize the movement of a highly mobile presequence peptide as bound to the mitochondrial import receptor, Tom20, and to estimate the solution conformation of a flexible loop segment in another mitochondrial import protein, Tim21, which was distorted in the conventional crystals by the crystal contacts.
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