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ヒト肺線癌の特性の分子病理学的解析

Research Project

Project/Area Number 01010082
Research Category

Grant-in-Aid for Cancer Research

Allocation TypeSingle-year Grants
Research InstitutionNational Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East

Principal Investigator

下里 幸雄  国立がんセンター, 病理部, 部長 (10076979)

Co-Investigator(Kenkyū-buntansha) 渡辺 慶一  東海大学, 医学部・病理学, 教授 (00055865)
関口 清俊  藤田学園保健衛生大学, 医学部・生化学教室, 講師 (50187845)
浜田 新七  滋賀医科大学, 第一病理学, 助手 (00172974)
桜井 雅温  埼玉県立がんセンター, 病院化学寮法部, 部長 (20025604)
横田 淳  国立がんセンター, がん転移研究室, 室長 (10191503)
Project Period (FY) 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥18,500,000 (Direct Cost: ¥18,500,000)
Fiscal Year 1989: ¥18,500,000 (Direct Cost: ¥18,500,000)
Keywordsヒトの肺 / 線癌 / 染色体異常 / 遺伝子異常 / 核DNAプロイディー / 基質糖蛋白 / 細胞接着分子
Research Abstract

肺小細胞癌でほぼ全例にみられた第3,13,17染色体の部分欠失を肺線癌で検討した結果,それぞれにつき原発巣では60ー100%に,脳転移では80ー100%に欠失を認めた。この所見は癌の発生あるいは悪性度増強には,組織型を問わず同じ遺伝子が関与していることを強く示唆している。
特に3p(-)は原発巣で全例に,かつ比較的早期にみられるので3pに癌抑制遺伝子があることが予想される。非小細胞癌75例につき染色体の核型分析をしたが,染色体数81以上の8症例はすべて線癌で,かつ3p(-)は扁平上皮癌により頻度が高かったことを除き,線癌・扁平上皮癌の二組織型間に差異を見いだすことができなかった。
DNA ploidy,mosaic ploidyの程度の予後との関連をT1NOMO25例とT1N2 29例についてみると,T1N2群でaneuploidyの頻度(p<0.01),mosaic ploidyの頻度(p<0.01),ならびに分裂指数(p<0.05)が有意に高く,肺線癌個々の管理方針の決定に核DNA定量が有効であると考えられた。また,原発巣,リンバ節転移,脳・肝転移についてみると,原発巣と胸郭内リンパ節転移との間に平均DNA量につき差を認めなかったが,脳転移巣,肝転移巣では,原発巣に比べて平均DNA量の大きな症例の頻度が有意に高かった。この所見は,原発巣玉で平均DNA量のより大きなsubcloneが出現し,これが血行転移成立に重要な役割を演じていることを示唆している。
フィブロネクチン(FN)mRNAのED-B可変域におけるスプライシングパターンを検討し,正常肺に比べ,線癌のみならず他の組織型の肺癌においてもED-B+mRNAの増加が確認された。肺癌組織におけるED-B+mRNAの増加は,ED-Bペプチドについての免疫組織学的な検討でも確認された。さらに,FN以下の基質糖蛋白も癌性変化を受けるか否かにつき検討したが,FNほど顕著な癌胎児性調節を受けるものは発見されなかった。

Report

(1 results)
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Noguchi,M,et al.: "Immunohistochemical distinction of malignant mesothelioma from pulmonary sdenocarcinoma with antisurfactant apoprotein,anti-Lewis^a,and anti-Tn antibodies." Hum.Pathol.20. 53-57 (1989)

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      1989 Annual Research Report
  • [Publications] Shimoyama,Y.,et al.: "Cadherin cell-adhesion molecules in human eqithelial tissues and carcinomas." Cancer Res.49. 2128-2133 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Asamura,H,et al.: "Nuclear DNA content by cytofluorometry of stage I adenocarcinoma of the oung in reoation to postoperative recurrence." Chest. 96. 312-318 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Asamura,H,et al.: "DNA cytofluorometric and nuclear morphometric analyses of lung adenocarcinoma." Cancer. 64. 1657-1664 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshida,K,et al.: "A cell kinetic study of pulmonary adenocarcinoma by an immunoperoxidase procedure after bromodeoxyuridine labeoing." Cancer. 64. 2284-2291 (1989)

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      1989 Annual Research Report
  • [Publications] Shiraishi,M.et al.: "Amplification of protooncogenes in surgical specimens of human lung carcinomas." Cancer Res.49. 6474-6479 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tsutsumi,K,et al.: "In situ hybridization and immunohistochemical study of human papillomavirus infection in adult laryngeal papillomas." Laryngoscope. 99. 80-85 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hirano,T,et al.: "In situ hybridization and immunohistochemical study of human papillomavirus infection in adult laryngeal papillomas." Jpn.J.Cancer Res.80. 348-355 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tsuda,H.,et al.: "Correlation between long-term survival in breast cancer patients and amplification of two putative oncogenes-coamplification units:hst-1/int-2 and c-erbB-2/ear-1.Cancer Res." 49. 1989. (3104-3108)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakamoto,M.,et al.: "Multicentric independent development of hepatocelluoar carcinama revealed by analysis of hepatitis B virus integration pattern." Am.J.Surg.Pathol.13. 1064-1067 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yokota,J.et al.: "Loss of heterozygosity on the short arm on chromosome 3 in carcinoma of uterine cervix." Cancer Res.49. 3598-3601 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Mori,N.,et al.: "Concordant deletions of chromosomes 3p and loss of heterozygosity for chromosomes 13 and 17 in small cell lung carcinomas." Cancer Res.49. 5130-5135 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hamada,S.,et al.: "Problem of size dependence in fluorescene DNA cytometry." Cytometry. 10. 394-401 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hamada,S.,et al.: "DNA ploidy and proliferative activity of human pulmonaty eqithelium." Viechows Arch B Cell Pathol.

    • Related Report
      1989 Annual Research Report
  • [Publications] Iwamori,M.,et al.: "Monoclonal antibody-defined antigen of human uterine endometrial carcinoma is le^b." J.Biochem.105. 718-722 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nozawa,S,et al.: "A monoclonal antibody(MSN-1)against a newly established uterine endometrial cancer cell line(SNG-II)and its application to immunohistochemistry and flow cytometry." Am.J.Obst.Gynecol.161. 1079-1086 (1989)

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      1989 Annual Research Report
  • [Publications] Nozawa,S.,et al.: "Human monoclonal antibody(HMST1)against lacto series type 1 chain and expression of the chain in uterine endometrial cancers." Cancer Res.49. 6401-6406 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kachiwagi,M.,et al.: "Significant increase in the antibody to Gall-3Gal structure in sera of patients with hepatocellular carcinoma after transcatheter arterial embolization." Cancer Res.49. 4396-4401 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Gamou,S.,et al.: "Subcellular localization of the EGF receptor maturation process." Exp.Cell Res.183. 197-206 (1989)

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      1989 Annual Research Report
  • [Publications] Gamou,S.,et al.: "Unique chromosomal location of amplified EGF receptor genes in EGF receptor-hyperproducing tumor cell line NA." Somat.Cell Mol.Genet.15. 179-184 (1989)

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      1989 Annual Research Report
  • [Publications] Hirai,M.et al.: "Lung caner cells often express high levels of protein kinase C activity." Jpn.J.Cancer Res.80. 204-208 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Oyama,F.,et al.: "Patterns of alternative spoicing of fibronectin pre-mRNA in human adult and fetal tissus." Biochemistry. 28. 1428-1434 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sekiguchi,K.,et al.: "Probing molecular polymorphism of fibronectins with antibodies directed to the alternatively spliced peptide segments." Biochemistry. 28. 3293-3298 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Oyama,F.,et al.: "Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors." J.Biol.Chem.264. 10331-10334 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Oyama,F.,et al.: "Oncodevelopmental regulation of the alternative splicing of fibronectin premessenger RNA in human lung tissues." Cancer Res.50. 1075-1078 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suemizu,H.,et al.: "Alterations in nonspecific cross-reacting antigen localization during cell culture. An immunoelectron microscopic study using a human lung adenocarcinoma cell line." Acta Pathol.Jpn.39. 772-778 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 桜井雅温: "肺癌の染色体異常とoncogene" 呼吸. 8. 1320-1324 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shimosato,Y.: "Diagnostic Surgical Pathology" Raven Press,New York, 875-827 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shimosato,Y.: "Basic and Clinical Concepts of Lung Cancer" Kluwer Academic Publishers,Boston, 71-87 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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