| Project/Area Number |
01041015
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Institution | Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University |
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Principal Investigator |
NISHIMURA Kazuko Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 真核微生物研究センター, 教授 (00114314)
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| Co-Investigator(Kenkyū-buntansha) |
李 秀芳 栄養及食品衛生研究所, 助理研究員
DAI Wen-li Div. of Dermatology Shandong Provincial Hospital, 皮膚科, 主任医師
李 若瑜 北京医科大学, 皮膚科, 助手
王 端礼 北京医科大学, 皮膚科, 教授
盂 昭赫 栄養及食品衛生研究所, 教授
HORIE Yoshikazu Natural History Museum and Institute, Chiba, 科長
TANAKA Reiko Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 真核微生物研究センター, 助手 (60143319)
TAGUCHI Hideaki Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 真核微生物研究センター, 助手 (70092070)
KURITA Nobuyuki Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 真核微生物研究センター, 助手 (90111434)
TERAO Kiyoshi Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 真核微生物研究センター, 教授 (30009120)
MIYAJI Makoto Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 真核微生物研究センター, 教授 (40009494)
LI Ruo-yu Dept. of Dermatology, First Hospital, Beijing Medical University
WANG Duan-li Dept. of Dermatology, First Hospital, Beijing Medical University
MENG Zhao-he Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine
LI Xiu-fang Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Pathogenic Dematiaceous Fungi / Ecology / China / Aspergillus / Aflatoxin / Sterigmatocystin / Rotting plant / Soil |
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| Research Abstract |
Ecological studies on pathogenic dematiaceous fungi and mycotoxin producing fungi in China
Chromomycosis is a chronic cutaneous and subcutaneous mycosis caused by a group of pathogenic dematiaceous fungi. It has been reported in many countries around the world, particularly from Middle and South Americas. Recently, it has been clarified that Shandong province and its neighboring areas in China are one of the endemic areas of this disease. In 1988 and 1989 we visited Shandog to investigate the ecology of the pathogenic dematiaceous fungi and mycotoxin producing fungi under the auspices of the Education Ministry of Japan. We collected various samples from nature and tried to isolate the fungi mentioned above at the laboratory of Department of Dermatology, Beijing Medical University and at the laboratory of the Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine.
we collected 273 soil samples and 510 ones consisting of rotting plant materials such as decayed whea
… More
t straws, wheat ears, corn stems, willow barks, etc. From these 783 samples, we succeeded in isolating the following pathogenic dematiaceous fungi.
Phialophora Verrucosa 63 : 49 From Rotting Plant Materials and 14 From Soil Samples.
Exophiala Dermatitidis 1 : From a Rotting Plant Material.
Exophiala jeanselmei
Exophiala Spinifera
Fonsecaea Pedrosoi
Alternaria Alternata
Veronaea Botryosa
All these fungi are the causative agents of chromomycosis in China and it is the first ecological study on the ecology of pathogenic dematiaceous fungi in China except for P. verrucosa.
As the study on mycotoxin producing fungi, we focused on the genus Aspergillus. Two hundreds and sixty-eight soil samples were collected from 11 areas in Shandong. Aspergilli were isolated at the laboratory of the Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine by using soil-plate method on Czapek's agar and incubating the plates at 37^ﾟC for 10 days. The most common species isolated from the soil samples were Aspergillus flavus, A. fumigatus and Emericella nidulans. Their numbers were 50, 193 and 119, respectively. Petromyces alliaceus was also isolated frequently. Its number was 22. From these isolates 44 A. flavus and 1 A. parasiticus were examined for production of aflatoxins. Thiry-two isolates of A. flavus produced aflatoxins ranging from trace to 32,000 mug per 1,000 ml of MYS medium. Concering sterigmatocystin, 26 out of 43 isolates of E. nidulans produced the mycotoxin ranging from 262.2 to 5,638.1 mug/1 Kg rice. Less
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