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The Role of Nucleotide Exchange in Regulation of Smooth Muscle Actin Polymerization.

Research Project

Project/Area Number 01044011
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionObihiro University of Agriculture and Veterinary Medicine.

Principal Investigator

KUWAYAMA Hideto  Obihiro University of Agriculture and Veterinary Medicine. Associate Professor., 畜産学部, 助教授 (40125399)

Co-Investigator(Kenkyū-buntansha) MIKI Masao  The University of Sydney. Department of Anatomy. Research Fellow., 解剖学部門, リサーチアソシエイト
DOS REMEDIOS C.G.  The University of Sydney. Department of Anatomy. Associate Professor., 解剖学部門, 助教授
Project Period (FY) 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Keywordsactin / ATP analogue / photoaffinity
Research Abstract

2-N_3ADP, a photoaffinity ATP analogue, was incorporated into the actin and the influence of the incorporation on the actin function was studied. The replacement of ADP with 2-N_3ADP in F-actin both before and after photo-cross-linking decreased appreciably the actin-activated S1-ATPase activity. Photocrosslinked 2-N_3ADP-F-actin could be depolymerized by dialysis against a solution containing 0.1 mM CaCl_2, 0.1 mM ATP and 1 mM Tris-HCl (pH 8.0). However, once it depolymerized, it lost very quickly the ability to polymerize even in the presence of a sufficient amount of ATP and Ca^<2+>.

Report

(1 results)
  • 1989 Final Research Report Summary
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 江藤真澄: "ミオシンのアクチン結合部位におけるアミノ酸側鎖の役割" 生物物理. 29. S333 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 桑山秀人: "Fーアクチンへのフォトアフィニティ-ATPアナログの導入とその効果" FEBS Letters. 250. 328-330 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Etou, Masumi et al.: "The function of amino acid residue around the actin binding site in myosin." Biophysics. 29. 333 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kuwayama, Hideto et al.: "The effect of the replacement of ADP with a photoaffinity ATP analogue, 2-azido-ADP, in F-actin on its function." FEBS Letters. 250. 328-330 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary

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Published: 1989-04-01   Modified: 2016-04-21  

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