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Regulation of gene expression during cell differentiation in the cellular slime molds

Research Project

Project/Area Number 01044080
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNational Institute for Basic Biology

Principal Investigator

TAKEUCHI Ikuo  National Institute for Basic Biology, 基礎生物学研究所, 所長 (90025239)

Co-Investigator(Kenkyū-buntansha) WILLIAMS J.  英国帝国癌研究所, 教授
TASAKA Masao  Kyoto University, Faculty of Science, 理学部, 助教授 (90179680)
JEFFREY G. Williams  Imperial Cancer Research Fund
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1989: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsCellular slime mold / Cell differentiation / Pattern formation / Cell sorting / Gene expression
Research Abstract

In the development of cellular slime molds, cells aggregate to form a tissue, in which two types of cells (prestalk and prespore) differentiate in the anterior and the posterior parts. To elucidate the mechanisms of cell differentiation and pattem formation, we made the following studies.
(1) We have shown that cells cultured in liquid in a roller tube form an agglutinate in which prestalk, and prespore cells exhibit a definite pattern of differentiation without undergoing morphogenesis. Williams made a similar culture of cells whose expression of prestalk-specific ecmA and ecmB genes can be histochemically detected by BATA-galactosidase activity. In the agglutinates forined, cells expressing ecmA first arose randomly and then were collected to the center, while those expressing ecmB appeared on the surface layers. The result is consistent with the previous one obtained with the pattem of prespore differentiation and indicates that the differentiation pattem is tonned by sorting out of differentiated cells.
(2) We have previously shown that the upstream region between -442 and -349 bp of presporespecific Dp87 gene is important for its expression. When this region is fused with the basal promoter of slime mold actin gene, the chimera gene was expressed from the vegetative stage and no difference in expression was detected between prestalk and prespore cells. This suggests that the upstream region only enhances the amount of transcription, but that cell-type-and development-specific expression is regulated by the region below -349 bp.
(3) Expression of Dp87 gene during normal development was examined by using cells transformed with the promoter of Dp87 fused with BATA-galactosidase gene. Cells expressing Dp87 first appeared randomly in the aggregation streams and were later accumulated in the basal part of the aggregation centers, continuing the above notion that the differentiation pattem is formed by sorting out of differentiated cells.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Morio,T.: "Transcriptional regulation of cellーtypeーenriched genes during development in Dictyostelium discoideum analyzed by nuclear runーon assays." Develop.Growth & Differ.33. 293-298 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tasaka,M.: "Developmental regulation of expression of presporeーspecific genes in Dictyostelium discoideum." Bot.Mag.Tokyo. 104. 359-370 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takemoto,K.: "cAMP regulation of the expression of prespore specific genes,SP96 and Dp87 in disaggregated slug cells of Dictyostelium discoideum." Cell Differentiation and Development. 31. 89-96 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tasaka,M.: "Isolation and characterization spore coat protein(Sp96)gene of Dictyostelium discoideum." Cell Differentiation and Development. 31. 1-9 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toda,K.: "Structure and expression of elongation facter 2 gene during development of Dictyostelium discoideum." J.Biol.Chem.264. 15489-15493 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeuchi,I.: "Formation of differentiation pattern in Dictyostelium discoideum." Genome. 31. 620-624 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeuchi,I.: "Cell sorting and pattern formation in Dictyostelium discoideum.In“Cellーcell interactions in early development"(J.Gerhart ed.)" WileyーLiss,Inc., 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹内 郁夫: "“細胞社会とその形成"(江口吾朗・鈴木義昭・名取俊二編)粘菌細胞による分化パタ-ン形成" 東京大学出版, 13 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Morio, T.: "Transcriptional regulation of cell-type-enriched genes during development in Dictyostelium discoideum analyzed by nuclear run-on assays." Develop. Growth & Differ.33. 293-298 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takemoto, K.: "cAMP regulation of the expression of prespore specific genes, SP96 and Dp87 in disaggregated slug cells of Dictyostelium discoideum." Cell Differentiation and Development. 31. 89-96 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tasaka, M.: "Isolation and characterization spore coat protein (Sp96) gene of Dictyostelium discoideum." Cell Differentiation and Development. 31. 1-9 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toda, K.: "Structure and expression of elongation factor 2 gene during development of Dictyostelium discoideum." J. Biol. Chem.264. 15489-15493 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeuchi, I.: "Formation of differentiation pattern in Dictyostelium discoideum." Genome. 31. 620-624 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeuchi, I.: Wiley-Liss, Inc.Cell sorting and pattern formation in Dictyostelium discoideum. In "Cell-cell interactions in early development" (J. Gerhart ed.), 249-259 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeuchi, I.: Tokyo Univ. Press. Formation of differentiation pattern by slime mold cells. (in Japanese) In "Cell society and its formation" (G. Eguchi, Y. Suzuki & S. Natori eds.), 87-99 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tasaka,M.: "Protein binding and DNase-I hypersensitive sites in the cis-acting regulatory region of the spore-coat SP96 gene of Dictyostelium." Mechanisms of Development. 36. 105-115 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Morio,T.: "Transcriptional regulation of cell-type-enriched genes during development in Dictyostelium discoideum analyzed by nuclear run-on assays." Develop.Growth & Differ.33. 293-298 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tasaka,M.: "Developmental regulation of expression of prespore specific genes in Dictyostelium discoideum." Bot.Mag.Tokyo. 104. 359-370 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takemoto,K.: "cAMP regulation of the expression of prespore specific genes,SP96 and Dp87 in disaggregated slug cells of Dictyostelium discoideum." Cell Differentiation and Development. 31. 89-96 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tasaka,M.: "Isolation and characterization spore coat protein (Sp96) gene of Dictyostelium discoideum." Cell Differentiation and Development. 31. 1-9 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takeuchi,I.(J.Gerhart ed.): "Cell sorting and pattern formation in Dictyostelium discoideum.In "Cell-cell interactions in early development"" Wiley-Liss,Inc., 11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takemoto,K.: "cAMP regulation of the expression of prespore specific genes,SP96 and Dp87 in disaggregated slug cells of Dictyostelium discoideum." Cell Differentiation and Development. 31. 89-96 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tasaka,M.: "Isolation and characterization spore coat protein (Sp96) gene of Dictyostelium discoideum." Cell Differentiation and Development. 31. 1-9 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toda,K.: "Structure and expression of elongation facter 2 gene during development of Dictyostelium discoideum." J.Biol.Chem.264. 15489-15493 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takeuchi,I: "Formation of differentiation pattern in Dictyostelium discoideum." Genome. 31. 620-624 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takeuchi,I.: "Cell sorting and pattern formation in Dictyostelium discoideum.In “Cellーcell interactions in early development" (J.Gerhart ed.)" WileyーLiss,Inc, ). 02%33 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹内 郁夫: "“細胞社会とその形成"(江口吾郎・鈴木義昭・名取俊二編) 粘菌細胞による分化パタ-ン形成" 13 (1989)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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