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Tyrosine-O-sulfation of Normal and Malignant Proteins

Research Project

Project/Area Number 01044119
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionMiyazaki University

Principal Investigator

NAKATSU Seiichiro  Miyazaki University Department of Biological Resource Science Professor, 農学部, 教授 (70040805)

Co-Investigator(Kenkyū-buntansha) DECKER Stuar  ロックフュラー大学, 生化学部, 助教授
MINGーCHEH Li  オクラホマ大学, 理学部, 准教授
NISHIYAMA Kazuo  Miyazaki University Department of Biological Resource Science Assistant Professo, 農学部, 助手 (40164610)
SUIKO Masahito  Miyazaki University Department of Biological Resource Science Associate Professo, 農学部, 助教授 (00128357)
LIU Ming-cheh  The University of Oklahoma, U. S. A. Department of Chemistry and Biochemistry As
STUART Decker J.  The Rockefeller University, U. S. A. Department of Biochemistry Assistant Profes
LIU MingーChe  オクラホマ大学, 理学部, 准教授
MINGーCHEN Li  オクラホマ大学, 理学部, 助教授
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 1991: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1990: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1989: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsTyrosine Sulfation / Posttranslational Modification / Golgi body / Bovine Liver / Sulfotransferase / Oligopeptide / Tyramine Sulfate / Receptor / ミクロゾ-ム / ヒルジン / オリゴビペプチド
Research Abstract

Post-translational protein modification by tyrosine sulfation has now been recognized to be widespread in animal cells and there is evidence indicating its involvement in modulation and modification of the biological activity of proteins, in influencing protein degradation, and in intracellular transport of proteins. Despite the considerable amount of effort made in recent years, the functional relevance of protein tyrosine sulfation, still, has not been fully elucidated. In an effort to verify the fate of the tyrosine sulfate from catabolized proteins in cells, the spent media of HepG2 human hepatoma cells and 3Y1 rat embryo fibroblasts labeled with[ ^<35>S]sulfate were analyzed by a two-dimensional thin-layer separation procedure. Autoradiographs of the cellulose thin-layer plate revealed the presense of tyramine-O-[ ^<35>S]Sulfate in addition to tyrosine-O-[ ^<35>S]sulfate in spent medium from human hepatoma cells. In contrast, only tyramine-O-[ ^<35>S]sulfate was observed in spent … More medium of 3Y1 rat fibroblasts. We could also recognize a 175 kDa membrane-bound protein in the bovine liver which exhibited binding affinity for tyrosine-O-sulfate (TyrS), suggesting a hypothesis of receptor mediated protein secretion using tyrosine sulfation for its signal, which was also supported by the fact that this membrane-bound TyrS-binding protein was found to be present in complex forms with several respective tyrosine-sulfated proteins, in freshly prepared bovine liver membrane lysate. Further we have purified and characterized a TyrS-binding protein (160 kDa) from bovine serum, which appears to be a good candidate as a putative carrier protein specific for tyrosine-sulfated proteins in the circulatory system, where the behavior of the tyrosine-sulfated proteins has not been elucidated. In vitro tyrosine sulfation of recombinant proteins would be a valuable tool in converting those proteins expressed in prokaryotic vectors to their natural form. For this purpose tyrosylprotein sulfotransferase (TPST) was characterized from a bovine liver Golgi preparation. Peptide substrates were synthesized and were sulfated with the TPST and Boc-Asp-Tyr-Val was found to be sulfated optimally. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] MingーCheh Liu: "TyrosineーOーSulfate,in Addition to TyrosineーOーSulfate,in Produced and Secreted by HePG2 Human Hepatoma Cells,But not by 3Y1 Embryo Fibroblasts." Biochemistry International. 21. 815-821 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "Quantitative Determination of TyrosineーOーSulfate in Urin: Application of IonーPair ReverseーPhase High Performance Liquid Chromatography in the Analysis of TyrosineーOーSulfate." Clinica.Chimica.Acta.193. 193-198 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "PostーTranslational Modification by Tyrosine Sulfation and Its Functions ー Does It Exist in Higher Plants" Proceeding of International Colloquium on Overcoming Breeding Barriers,Miyazaki. 59-65 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] MingーCheh Liu: "Possible Involvenent of Protein Tyrosine Sulfation in the Polarized Protein Sorting in FilterーGrown MadinーDarby Canine Kidney(MDCK)Cells." Proceeding of International Colloquium on Overcoming Breeding Barriers,Miyazaki. 67-68 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] MingーCheh Liu: "Identification of Complexes between the TyrosineーOーSulfateーBinding Protein and TyrosineーSulfated Proteins in Bovine Liver Membrane Lysate." Biochemical Journal. 275. 259-262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Jian Rong Han: "PostーTranslational Modification and Binding Properties of the Apically Secreted 80 KDa Glycoーprotein from Madin Derby Canine Kidney Cells:" Archives of Biochemistry and Biophysics. 286. 337-345 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ruiliang Lu: "Purification and Characterization of a Membrane Bound TyrosineーOーSulfateーBinding Protein from Bovine Liver." Archives of Biochemistry and Biophysics. 286. 481-487 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuji Kishima: "Betalain Pigmentation in Petal of Portulaca is Preceded by a Tyrosine Accumulation." Journal of Plant Physiology. 137. 505-506 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] P.H.Prasantha Fernando: "Protein Tyrosine Sulfation in Animal Cells and Its Simulation in vitro in the Downstream Processing of Proteins." Proceeding of the Fourth Annual Meeting and International Symposium of Japanese Association For Animal Cell Technology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] MingーCheh Liu: "Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Sorting in FilterーGrown MadinーDarby Canine Kidney(MDCK)Cells." Proceeding of the Fourth Annual Meeting and International Symposium of Japanese Association For Animal Cell Technology.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] MingーCheh Liu: "Identification of a Putative TyrosineーOーSulfate Receptor and Its Presence in Complex Form with a Major TyrosineーSulfated Protein,the 80 KDa Glycoprotein in MadinーDarby Canine Kidney Cells." Journal of Biological Chemistry. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] P.H.Prasantha Fernando: "Isolation and Characterization of a Novel(Microsomal)Membraneーbound Phenol Sulfotransーferase from Bovine Liver." Biochem.Biophys.Res.Commun.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoichi Sakakibara: "Purification and Characterization of TyrosineーOーsulfateーbinding Protein from Bovine Serum." Arch.Biochem.Biophys.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "The Role of Tyrosine Sulfation in Protein: Activities of Several Sulfatases on Tyrosine Sulfated Protein and Related Compounds." Journal Biochemistry. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "Postーtranslational Protein Modification by Tyrosine Sulfation: Biochemistry and Functional Role of a Bovine Liver TyrosineーOーSulfateーbinding Protein.The Postーtranslational Modification of Proteins" Japan Science Society Press,Tokyo, 19-34 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ming-Cheh Liu: "Tyramine-O-Sulfate, in Addition to Tyrosine-O-Sulfate, is Produced and Secreted by HepG 2 Human Hepatoma Cells, But not by 3Y1 Rat Embryo Fibroblasts." Biochem. International. 21. 815-821 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "Quantitative Determination of Tyrosine-O-sulfate in Urine : Application of Ion-Pair Reverse-Phase High Performance Liquid Chromatography in the Analysis of Tyrosine-O-sulfate." Clinica. Chimica. Acta.193. 193-198 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "Post-Translational Modification by Tyrosine Sulfation and Its Functions-Does It Exist in Higher Plants?" Proceeding of International Colloquim on Overcoming Breeding Barriers, Miyazaki.59-65 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ming-Cheh Liu: "Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Sorting in Filter-Grown Madin-Darby Canine Kidney (MDCK) Cells." Proceeding of International Colloquim on Overcoming Breeding Barriers, Miyazaki. 67-68 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ming-Cheh Liu: "Identification of Complexes between the Tyrosine-O-Sulfate-Binding Protein and Tyrosine-Sulfated Proteins in Bovine Liver Membrane Lysate." Biochem. J.275. 259-262 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "The Post-translational Modification of Proteins" Japan Sci. Soc. Press, Tokyo. 19-34 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Jian Rong Han: "Post-Translational Modification and Binding Properties of the Apically Secreted 80 KDa Glycoprotein from Madin Derby Canine Kidney Cells : Similarities to the C-Terminal Portion of the Basolaterally Secreted Fibronectin." Arch. Biochem. Biophys.286. 337-345 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ruiliang Lu: "Purification and Characterization of a Membrane Bound Tyrosine-O-Sulfate-Binding Protein from Bovine Liver." Arch. Biochem. Biophys.286. 481-487 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuji Kishima: "Betalain Pigmentation in Petal of Portulaca is Preceded by a Dramatic Tyrosine Accumulation." J. Plant Physiol.137. 505-506 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] P. H. Prasantha Fernand: "Protein Tyrosine Sulfation in Animal Cells and Its Simulation in vitro in the Downstream Processing of Proteins." Proceeding of the Fourth Annual Meeting and International Symposium of Japanese Association For Animal Cell Technology.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ming-Cheh Liu: "Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Sorting in Filter-Grown Madin-Darby Canine Kidney (MDCK) Cells." Proceeding of the Fourth Annual Meeting and Inter national Symposium of Japanese Association For Animal Cell Technology.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ming-Cheh Liu: "Identification of a Putative Tyrosine-O-Sulfate Receptor and Its Presence in Complex Form with a Major Tyrosine-Sulfated Protein, the 80 KDa Glycoprotein, in Madin-Darby Canine Kidney Cells." J. Biol. Chem.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] P. H. Prasantha Fernando: "Isolation and Characterization of a Novel(Microsomal)Membrane-Bound Phenol Sulfotransferase from Bovine Liver." Biochem. Biophys. Res. Commun.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoichi Sakakibara: "Purification and Characterization of Tyrosine-O-sulfate-binding Protein from Bovine Serum." Arch. Biochem. Biophys.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: "The Role of Tyrosine Sulfation in Protein : Activities of Several Sulfatases on Tyrosine Sulfated Protein and Related Compounds." J. Biochem.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahito Suiko: Japan Sci. Soc. Press, Tokyo. The Post-translational Modification of Proteins, 19-34 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ming-Chen Liu: "Identification of Complexes between the Tyrosine-O-Sulfate-Binding Protein and Tyrosine-Sulfated Proteins in Bovine Liver Membrane Lysate." Biochemical Journal. 275. 259-262 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Jian Rong Han: "Post-translational Modification and Binding Properties of the Apically Secreted 80 KDa Glycoprotein from Madin Darby Canine Kidney Cells." Archives of Biochemistry and Biophysics. 286. 337-345 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ruiliang Lu: "Purification and Characterization of a Membrane bound Tyrosine-O-Sulfate-Binding Protein from Bovine Liver." Archives of Biochemistry and Biophysics. 286. 481-487 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yuji Kishima: "Betalain Pigmentation in Petal of Portulaca is Preceded by a Dramatic Tyrosine Accumulation." Journal of Plant Physiology. 137. 505-506 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] P.H.Prasantha Fernando: "Protein Tyrosine Sulfation in Animal Cells and Its Simulation in vitro in the Downstream Processing of Proteins." Proceeding of the Fourth Annual Meeting and International Symposium of Japanese Association for Animal Cell Technology.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ming-Cheh Liu: "Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Sorting in Filter-Grown Madin-Darby Canine Kidney(MDCK)Cells." Proceeding of the Fourth Annual Meeting and International Symposium of Japanese Association for Animal Cell Technology.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Masahito Suiko: "The Post-translational Modification of Proteins." Japan Sci.Soc.Press,Tokyo, 19-34 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] MingーChen Liu: "TyramineーOーSurfate,in Addition to TyrosineーoーSulfate,is Produced and Secreted by HepG2 Human Hepatoma Cells,but not by 3Yl Rat Embryo Fibroblasts." BioChemistry International. 21. 815-821 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Jian Rong Han: "PostーTranslational Modifications and Binding Properties of the Aplically Secreted 80ーKDa Glycoprotein from MadinーDarby Canine Kidney Cells: Similarities to the CーTerminal Portion of the Basolaterally Secreted Fibronectin." Archives of Biochemistry and BioPhysics. 286. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ruiliang Lu: "Purification and Characterization of a MembraneーBound TyrosineーOーSulfateーBinding Protein from Bovine Liver." Archives of Biochemistry and BioPhysics. 286. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masahito Suiko: "Quantitative Determination of TyrosineーOーsurfate in Urine: Application of IonーPair ReverseーPhase High Performance Liquid Chromatography in the Analysis of TyrosineーOーsulfate." Clinica.Dhimica.Acta. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masahito Suiko: "The Role of Tyrosine Sulation in Protein: Activities of Several Surfatases on Tyrosine Sulfated Proteins and Related Compounds." Biochemical Journal. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masahito Suilo: "PostーTranslational Modification by Tyrosine Sulfation and Its Functions ー Does It Exist in Higher in Plants?" Proceedings of the International Colloquium on Overcoming Breeding Barriers,Miyazaki,March 11ー15.59-65 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] MingーCheh Liu: "Identification of Complexes between the TyrosineーOーSulfateーBinding Protein and TyrosineーSulfated Proteins in Bovine Liver Membrane Lysate." Biochemical Journal. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yuji Kishima: "Betalain Pigmentation in Petal of Portulaca is Preceded by a Dramatic Tyrosine Accumulation."

    • Related Report
      1990 Annual Research Report
  • [Publications] MingーCheh Liu: "Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Sorting in FillterーGrown MadinーDarby Canine Kidney (MDCK) Cells." Proceedings of the International Colloquium on Overcoming Breeding Barriers,Miyazaki,March,11ー15.67-68 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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