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Photosynthesis and Photobiological Studies of Microalgae

Research Project

Project/Area Number 01045009
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionThe University of Tokyo

Principal Investigator

TSUZUKI Mikio (1990-1991)  Institute of Applied Microbiology, The University of Tokyo, 応用微生物研究所, 助教授 (70155430)

宮地 重遠 (1989)  東大, 応用微生物研究所, 教授

Co-Investigator(Kenkyū-buntansha) 宮地 重遠  東京大学, 応用微生物研究所, 所長 (40013312)
WEHRMEYER W.  Faculty of Biology, Marburg Philipps-University, フィリップス大学・生物学部, 教授
THAUER R. K.  Faculty of Biology, Marburg Philipps-University, フィリップス大学・生物学部, 教授
NULTSCH W.  Faculty of Biology, Marburg Philipps-University, フィリップス大学・生物学部, 教授
GALLAND P.  Faculty of Biology, Marburg Philipps-University, フィリップス大学・生物学部, 助教授
SENGER Horst  Faculty of Biology, Marburg Philipps-University, フィリップス大学・生物学部, 教授
IGARASHI Yasuo  Faculty of Agriculture, The University of Tokyo, 農学部, 助手 (90114363)
OHMORI Masayuki  Ocean Research Institute, The University of Tokyo, 海洋研究所, 助教授 (80013580)
WATANABE Tadashi  Institute of Industrial Science, The University of Tokyo, 生産技術研究所, 助教授 (70092385)
KATO Sakae  Faculty of Science, The University of Tokyo, 理学部, 教授 (50011515)
HORST Senger  マールブルグ, フィリップス大学・生物学部, 教授
SENGE M.  マールブルグ, フィリプス大学・生物学部, 助教授
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsMicroalgae / Chlorophyll / cAMP / Carbon dioxide / Rubisco / Photosystem I / Photosignal transduction / Thermophile / 反応中心 / ラン藻 / Thermophile / RuBisCO / GTP結合タンパク質 / 環境要因 / クロロフィル合成 / らん藻 / 光信号受容タンパク質 / 細胞膜 / 光合成
Research Abstract

The former head of this research program, Shigetoh Miyachi, cooperated with Prof. Senger and showed that quantum efficiency in photosynthesis of some green algae grown under ordinary air (low-CO^2 cells) was lower than the cells grown under 3% CO^2 (high-CO^2 cells) and that chlorophyll fluorescence at low temperature at 710-740 nm was higher than that at 680-695 nm in low-CO^2 cells. These results suggests that photosystem I is more active in low-CO^2 cells than in high-CO^2 cells. They also showed Ca^<2+> requirement in the activation of protochlorophyllide oxidoreductase. Tsuzuki investigated the conversion from chlorophyll a to b using Scenedesmus mutant with Prof. Senger. Ohmori found the existence of cAMP and GTP-binding protein in-cyanobacteria and showed remarkable changes in the levels of cAMP by the exposure to light. During the experiments, he discussed the data the mechanism of signal transduction by light in cyanobacteria with Prof. Senger and Dr. Galland. They also considered protein as a possible photoreceptor. Still, he discussed a possibility of electron microscope for determination of photoreceptor protein with Prof. Wehrmeyer. Watanabe carried out experiments with Prof. Senger to show the ratio of two photosystems at various wavelength, light intensity, and CO^2 concentration in cyanobacteria. The ratio of PS I over II was changed by the former two factors, but little by CO^2 concentration. They also discussed the possible function of chlorophyll a' observed in photosystem I reaction center. It was good for Watanabe to obtain a prospect for characterization of chlorophyll a' dimmer prepared artificially. Igarashi has isolated Rubisco from a thermophilic cyanobacterium and found that the Rubisco is stable at high temperature. He discussed the data as well as characteristics of CO^2 fixation of microorganisms grown under extreme environment with Prof. Thauer and talked about future cooperation.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] K.Kotzabasis: "Influence of calcium on formation and reduction of protochlorophyllide in the pigment mutant C-2A' of Scenedesmus obliquus." Plant Cell Physiol.31. 419-422 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Tsuzuki: "Effects of CO_2 concentration during growth on fatty acid composition in microalgae." Plant Physiol.93. 851-856 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Tsuzuki: "CO_2 syndrome in Chlorella." Can.J.Bot.69. 1003-1007 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Yaguchi: "Purification of RuBisCO from the termophilic cyanobacterium Synechococcus sp.strain a-1." J.Ferment.Bioeng.73. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Ishii: "Methionaquinone is a direct natural electron acceptor for the membrane-bound hydrogenase in Hydrogenobacter thermophilus strain TK-6." Agric.Biol.Chem.55. 3011-3016 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Ohmori: "Ammonium-nitrogen partitioning under different balances of nitrogen and carbon in the cyanobacterium Anabaena cylindrica." J.Gen.Appl.Microbiol.36. 171-177 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Ohmori: "Function of cAMP as a mat-forming factor in the cyanobacterium Spirulina platensis." Plant Cell Physiol.33. 21-25 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Ohmori: "cAMP and cAMP-stimulated protein phosphorylation in zoospores of brown algae." J.Phycol.27. 540-543 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Sakamoto: "The concentration of AMP and adenylate cyclase activity in cyanobacteria." Plant Cell Physiol.32. 581-584 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Kobayashi: "Enrichment of Bacteriochlorophyll g' in membranes of Heliobacterium chlorum by ether extraction:unequivocal evidence for its presence in vivo." FEBS Letters. 284. 129-131 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] E.J.van de Meent: "Identification of 8'-hydroxychlorophyll a as a functional reaction center pigment in Heliobacteria." Biochim.Biophys.Acta. 1058. 356-362 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Maeda: "Presence of two chlorophyll a' molecules at the core of photosystem I." Biochim.Biophys.Acta. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Omata(分担執筆): "Inorganic Nitrogen in Plants and Microorganisms." SpringerーVerlag, 86-92 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Watanabe(分担執筆): "Chlorophylls" CRC Press, 1-1257 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 五十嵐 泰夫(分担執筆): "極限環境微生物ハンドブック:炭酸固定と利用" サイエンスフォ-ラム社, 376-384 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Kotzabasis: "Influence of calcium on formation and reduction of protochlorophyllide in the pigment mutant C-2A' of Scenedesmus obliquus." Plant Cell Physiol.31. 419-422 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Tsuzuki: "Effects of CO^2 concentration during growth on fatty acid composition in microalgae." Plant Physiol.93. 851-856 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Tsuzuki: "CO^2 syndrome in Chlorella." Can. J. Bot.69. 1003-1007 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Yaguchi: "Purification of RuBisCO from the thermophilic cyanobacterium Synechococcus sp. strain a-1." J. Ferment. Bioeng.73. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Ishii: "Methionaquinone is a direct natural electron acceptor for the membrane-bound hydrogenase in Hydrogenobacter thermophilus strain TK-6." Agric. Biol. Chem.55. 3001-3016 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Ohmori: "Ammonium-nitrogen partitioning under different balances of nitrogen and carbon in the cyanobacterium Anabaena cylindrica." J. Gen. Appl. Microbiol.36. 171-177 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Ohmori: "Function of cAMP as a mat-forming factor in the cyanobacterium Spirulina platensis." Plant Cell Physiol.33. 21-25 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Ohmori: "cAMP and cAMP-stimulated protein phosphorylation in zoospores of brown algae." J. Phycol.27. 540-543 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Sakamoto: "The concentration of AMP and adenylate cyclase activity in cyanobacteria." Plant Cell Physiol.32. 581-584 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Kobayashi: "Enrichment of bacteriochlorophyll g' in membranes of Heliobacterium chlorum by ether extraction : unequivocal evidence for its presence in vivo." FEBS Letters. 284. 129-131 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] E. J. van de Meent: "Identification of 8'-hydroxychlorophyll a as a functional reaction center pigment in Heliobacteria." Biochim. Biophys. Acta. 1058. 356-362 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Maeda: "Presence of two chlorophyll a' molecules at the core of photosystem I." Biochim. Biophys. Acta. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Omata: Springer-Verlag. Inorganic Nitrogen in Plants and Microorganisms., 86-92 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Watanabe: CRC Press. Chlorophylls, 1-1257 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Igarashi: Science Forum Inc., Ltd.Handbook of microbiology in extreme environment (Japanese), 376-384 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Kobayashi: "Enrichment of bacteriochlorophyll g' in membrances of Heliobacterium chlorum by ether extraction:Unequinocal evidence for its presence in vivo." FEBS Letters. 284. 129-131 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] E.J.vande Meent: "Identification of 8'-hydroxy chlorophyll a as a functional reaction center pigment in Heliobacteria." Biochim.Biophys.Acta. 1058. 356-362 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Yaguchi: "Purification of RuBisCO from the thermophilic cyanobacterium Synechococcus sp.strain a-1." J.Ferment.Bioceng.73. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Ishii: "Methionaquinone is a direct natural electron acceptor for the membrane-bound hydrogenase in Hydrogenobactor thermophilcs strain Tk-6" Agric.Biol.Chem.55. 3011-3016 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Ohmori: "Function of cAMP as a mat-forming factor in the cyanobacterium Spirulina platensis." Plant Cell Physiol. 33. 21-25 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Ohmori: "cAMP and cAMP-stimulated protein phosphorylation in zoospores of brown algae." J.Phycol.27. 540-543 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Watanabe(分担執筆): "Chlorophylls" CRC Press(米国), 1-1257 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 五十嵐 泰夫(分担執筆): "極限環境微生物ハンドブック:炭酸固定と利用" サイエンス・フォ-ラム社, 376-384 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Kotzabasis: "Influence of calcium on formation and reduction of protochloropyhllide in the pigment mutant Cー2A'of Scenedesmus obliquus." Plant Cell Physiol.31. 419-422 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Ohmori: "Ammoniumーnitogen partitioning under different balances of nitrogen and carbon in the cyanobacterium Anabaena cylindrica." J.Gen,Appl.Microbiol. 36. 171-177 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Tsuzuki: "Effects of CO_2 concentration during growth on fatty acid composition in microalgae." Plant Physiol.93. 851-856 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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