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Studies on Antimutagenic and Antitumoral Activities of Microbial Polysaccharides

Research Project

Project/Area Number 01303011
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 畜産化学
Research InstitutionShinshu University

Principal Investigator

HOSONO Akiyoshi  Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (20021074)

Co-Investigator(Kenkyū-buntansha) OTANI Hajime  Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (30109201)
KATAOKA Kei  Okayama University, Faculty of Agriculture, Professor, 農学部, 教授 (90032987)
YAMANAKA Yoshitada  Tokyo University of Agriculture, Faculty of Agriculture, Professor, 農学部, 教授 (80078112)
WATANABE Kenji  Gifu University, Faculty of Agriculture, Professor, 農学部, 教授 (70023447)
ITOH Takatoshi  Tohoku University, Faculty of Agriculture, Professor, 農学部, 教授 (80005610)
古川 徳  東京農業大学, 農学部, 講師 (10078149)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1990: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsMicrobial Polysaccharides / Antimutagenicity / Antitumor / Adjuband Effect / Fermented Milk / Superoxide Dismutase / Lactic Acid Bacteria / 微生物生産多糖類 / ケフィ-ル / ダディヒ / アジェバンド効果 / マイドジェル活性
Research Abstract

Throughout 2 years research regarding to the subject described above, we obtained the following results.
(1) Preparation of mutant strains with high producing abilities of polysaccharides :
Mutant strains with high producing abilities of polysaccharides were prepared from the bacterial strains isolated from various kinds of fermented milk. Polysaccharides prepared from these mutant strains showed higher antitumoral activities compared with original strains.
(2) Adjuvand effect of microbial polysaccharides :
We confirmed the immunopotentiating activity on Mycobacterium tuberculosis, and detected little activity on Leuconostoc Paramesenteroides and Streptococcus lactis subsp. Diacetylactis.
(3) Superoxide dismutase (SOD) activity :
SOD activities of several strains of lactic acid bacteria was investigated. Among the strains tested St. salivarius subsp. Thermophilus demonstrated highest SOD production. General properties of partially purified SOD produced by this strain were also investigated.
(4) Antimutagenic and antitumoral activities of polysaccharides prepared from the strains in traditional fermented milk products :
Various strains of lactic acid bacteria were isolated from various types of traditional fermented milk products from Asian and East European countries. Peptideglycan fractions obtained from Leu. Dextranicum and Leu. Paramesenteroides demonstrated highest antimutagenic activity toward Trp-P1 and Trp-2. The kinetics of such amino acid pyrolyzates was found to be bandage to peptidoglycans. Polysaccharides prepared from Lactobacillus jugruti and Lb. Helveticus showed inhibition against the growth of Lewis lung carcinoma and Sarcoma-180. Chemical composition of polysaccharides prepared from these was also examined.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Akiyoshi HOSONO,Robertus WARDOJO and Hajime OTANI: "Binding of Amino Acid Pyrolyzates by Lactic Acid Bacteria Isolated from “Dadih"." Lebensm.‐Wiss.u.Technol.23. 149-153 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO,Tsutomu TANABE and Hajime OTANI: "Binding Properties of Lactic Acid Bacteria Isolated from Kefir Milk with Mutagenic Amino Acid Pyrolyzates." Milchwissenschaft. 45. 647-651 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyosihi HOSONO,Robertus WARDOJO and Hajime OTANI: "Inhibitory Effects of Lactic Acid Bacteria from Fermented Milk on the Mutagenicities of Volatile Nitrosamins." Agric.Biol.Chem.54. 1639-1643 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO Haruko Yamazaki and Hajime OTANI: "Antimutagenicity of Slimy Substance Separated from the Culture of Brevibacterium linens." Jpn.J.Zootech.Sci.60. 679-685 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO,Kohei MAKINO and Hajime OTANI: "Mutagenicity of Resorcinol Fermented by the Reaction of m‐Phenylenediamine with Sodium Nitrite." J.Agric.and Food Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO,Masafumi SUZUKI and Hajime OTANI: "Superoxid Dismutage Activty in the Crude Cell Extract from Streptococcus salivarius subsp.thermophilus 3535." Anim.Sci.Technol.(Jpn.). 62. 39-44 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hajime OTANI and Akiyoshi HOSONO: "The Chicken Egg Yolk Antibody Level as an Indicator to Assess Adjuvant Activity." Jpn.J.Dairy and Food Sci.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Haruki KITAZAWA,Takahiro TOBA,Takatoshi ITOH,Nobuko KUMANO,Susumu ADACHI and Takahiro YAMAGUCHI: "Antitumoral Activity of SlimeーForming Encapsulated Lactococcus lactis subsp.cremoris Isolated from Scandinavian Ropy Sorr Milk,“Viili"." Jpn.J.Zootech.Sci.62. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hajime NAKAJIMA,Shuuji TOYODA.Takahiro TOBA,Takatoshi ITOH.Haruki KITAZAWA and Susumu ADACHI: "A Novel Phosphopolysaccharid from SlimーForming Lactococcus lactis subspecies cremoris SBT 0495." J.Dairy Sci.73. 1472-1477 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hajime NAKAJIMA,Tetsuji HIROTA,Takahiro TOBA,Takatoshi ITOH and Susumu ADACHI: "Structure of the Extracellular Polysaccharide Viilian,from SlimーForming Lactococcus lactis subsp.cremoris SBT 0495." Carbohyd.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 山中 良忠: "抗腫瘍性多糖生菌の分離"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 古川 徳: "微生物生産多糖投与による in vivoの抗腫瘍性発現の検討"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 渡辺 乾二: "食品有用酵母の産生する酸性多糖とその生理機能"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 渡辺 乾二: "食餌性コレステロ-ルと健康" 日食工誌. 37. 830 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮本 拓、片岡 啓: "ケフィ-ルの構成乳酸菌とその抗変異原性" 酪科食研.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO, Robertus WARDOJO and Hajime OTANI: "Binding of Amino Acid Pyrolyzates by Lactic Acid Bacteria Isolated from "Dadih"" Lebensm. Wiss. u. Technol.23. 149-153 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO, Tsutomu TANABE and Hajime OTANI: "Binding Properties of Lactic Acid Bacteria Isolated from Kefir Milk with Mutagenic Amino Acid Pyrolyzates." Milchwissenschaft. 45(10). 647-651 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO, Robertus WARDOJO and Hajime OTANI: "Inhibitory Effects of Lactic Acid Bacteria from Fermented Milk on the Mutagenicities of Volatile Nitrosamines." Agric. Biol. Chem.54(7). 1639-1643 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO, Haruko YAMAZAKI and Hajime OTANI: "Antimutagenicity of Slimy Substance Separated from the Culture of Brevibacterium linens." Jpn. J. Zootech. Sci.60(7). 679-685 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi NOSONO, Kohei MAKINO and Hajime OTANI: "Mutagenicity of Resorcinol Fermented by the Reaction of m-Phenylenediamine with Sodium Nitrite." J. Agric. and Food Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akiyoshi HOSONO, Masafumi SUZUKI and Hajime OTANI: "Superoxide Dismutage Activity in the Crude Cell Extract from Streptococcus salivarius subsp. thermophilus 3535." Anim. Sci. Technol. (Jpn.). 62(1). 39-44 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hajime OTANI and Akiyoshi NOSONO: "The Chicken Egg Yolk Antibody Level as an Indicator to Assess Adjuvant Activity." Jpn. J. Dairy and Food Sci.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Haruki KITAZAWA, Takahiro TOBA, Takatoshi ITOH, Nobuko KUMANO, Susumu ADACHI and Takahiro YAMAGUCHI: "Antitumoral Activity of Slime-Forming Encapsulated Lactococcus lactis subsp. cremoris Isolated from Scandinavian Ropy Sour Milk, "Viili"." Jpn. J. Zootech. Sci.62. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hajime NAKAJIMA, Shuuji TOYODA, Takahiro TOBA, Takatoshi ITOH, Haruki KITAZAWA and Susumu ADACHI: "A Novel Phosphopolysaccharide from Slime-Forming Lactococcus lactis subspecies cremoris SBT 0495." J. Dairy Sci.73. 1472-1477 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hajime NAKAJIMA, Tetsuji HIROTA, Takahiro TOBA, Takatoshi ITOH and Susumu ADACHI: "Structure of the Extracellular Polysaccharide Viilian, from Slime-forming Lactococcus lactis subsp. cremoris SBT 0495." Carbohyd. Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshitaba YAMANAKA: "Isolation of Lactic Acid Bacteria Produced Antitumoral Polysaccharide."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Noboru FURUKAWA: "Antitumor Effects of Extracellular Polysaccharide Produced by Lactic Acid Bacteria on Tumor Bearing Mice in Vivo."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kenji WATANABE: "Dietary Cholesterol and Human Health." Npn. Shokuhin Kogyo Gakkaishi. 37(10). 830-837 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hosono,A.,Wardojo,R.,Otani,H.: "Inhibitory effect of lactic acid bacteria from fermented milk on the mutagenicities of volatile nitrosamines." Agric.Biol.Chem.54. 1639-1643 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hosono,A.,Tanabe,T.,Otani,H.: "Binding properties of lactic acid bacteria isolated from Kefir milk with mutagenic amino acid pyrolyzates." Milchwissenschaft. 45. 647-651 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hosono,A.,Makino,K.,Otani,H.: "Mutagenicity of resorcinol formed by the reaction of mーphenylendiamine with sodium nitrite." J.Agric.and Food Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakajima,H.,Toyoda,S.,Toba,T.,Itoh,T.,Kitazawa,H.,Adachi,S.: "A nobel phosphopolysaccharide from slimeーforming Lactococcus lactis subsp.cremoris SBT 0495." J.Dairy Sci.73. 1472-1477 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hosono,A.,Suzuki,M.,Otani,H.: "Superoxide dismutase activity in the crude cell extract from Streptococcus salivarius subsp.thermophilus 3535." Anim.Sci.Technol.(Jpn.). 62. 39-44 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Otani,H.,Hosono,A.: "The chicken egg yolk antibody leel as an indicator to assess adjuvant activity." Jpn.J.Dairy and Food Sci.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hosono,A.et al.: "Microbial flara in "dadih",a traditional fermented milk in Indonesia." Lebensmitt.-Wiss.u.Tech.22. 20-24 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 西岡功志ら: "抗変異原性乳酸菌の検索に関する予備的検討" 日畜会報. 60. 491-494 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hosono,A.et al.: "Binding of amino acid pyrolyzates by lactic acid bacteria isolated from"dadih"." Lebensmitt.-Wiss u.Tech. 23. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hosono,A.et al.: "Inhibitory effects of lactic acid bacteria encountered in a fermented milk on themutagenicities of volatile nitrosamines." Agri.Biol.Chim.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hosono,A.et al.: "Bibding properties of lactic acid bacteria isolated from kefir milk with mutagenic amino acid pyrolyzates." Milchwissenschaft.45. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakajima,H.et al.: "A novel phosphopolysaccharide from slime-forming Lactococcus lactis subsp.cremoris SBT 0495." J.Dairy Sci.73. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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