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Regulatory Mechanisms of Cell Division in Early Development

Research Project

Project/Area Number 01304005
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionHokkaido University

Principal Investigator

KATAGIRI Chiaki  Hokkaido Univ. FAC. SCI., PROF., 理学部, 教授 (90000827)

Co-Investigator(Kenkyū-buntansha) SAWAI Tsuyoshi  Yamagata Univ., COLL. GEN. EDUC., PROF., 教養部, 教授 (10007239)
MABUCHI Issei  Univ. Tokyo, COLL. ARTS, SCI., ASS. PROF., 教養学部, 助教授 (40012520)
KISHIMOTO Takeo  Tokyo Inst. TECH. FAC. SCI., PROF., 理学部, 教授 (00124222)
NEMOTO Shin-ichi  Ochanomizu Univ. FAC. SCI., ASS. PROF., 理学部, 助教授 (60017209)
米田 満樹  京都大学, 理学部, 教授 (50017183)
浜口 幸久  東京工業大学, 理学部, 教授 (70016161)
清水 隆  北海道大学, 理学部, 助教授 (50113569)
平本 幸男  放送大学, 教養学部, 教授 (50011440)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 1990: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1989: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordsGerminal Vesicle / Maturation Promoting Factor / Cyclin / Nucleoplasmin / Hi Histone Subtype / Contractile Ring / Actin / Cleavage Furrow / cdc2蛋白質 / 精子前核 / 抗チュ-ブリン抗体 / 分裂溝 / S期 / 卵成熟分裂 / 卵熟成促進因子 / 分裂装置 / 表層細胞質 / アクチン繊維
Research Abstract

A technique was devised to isolate a large number of germinal vesicles (GV) from the starfish oocytes, with which the system was established for biochemical analyses of cyclic cytoplasmic activities that accompany cell cycles during maturation and cleavage divisions. Tha maturation promoting factor (MPF) in starfish oocytes is defined as the histone H1 kinase activity which is based on the phosphorylated cdc2 protein associated with the cyclin. The cyclic proteolysis of cyclin results in cessation of M-phase in cell cycles. Remodeling of nuclear basic proteins during fertilization and cleavage stages was analyzed by the cell-free system employing lysolecithin-permeabilized sperm nuclei incubated in the cytosls of amphibian eggs. During pronulcear formation, the protamines are quickly removed non-enzymatically by the nucleoplasmin derived from the GV, followed by an addition of nucleosomal core histones and a subtype of histone H1 (H1X) which is specific to the cleavage stage nuclear chromatin. The contractile ring occurring during cytokinesis of sea urchin eggs is formed by an organized assembly of actin fibers which is based on the phosphorylated myosin light chains. Of several protein s that are thought to participate in regulation of contractile ring formation, determinations were made of the binding site on the actin molecule of depactin as well as the primary structure of profilin, an inhibitor of G-actin polymerization. Both the cleavage furrow-inducing activity and the furrow formation in the amphibian eggs were inhibited by colchicine and vinblastine, suggesting a direct involvement of microtubule system in the formation of contractile system of the cleavage furrow.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Yokota,T.,K.Takamune & Ch.Katagiri: "Nuclear basic proteins of <Xenopus laevis>___ー sperm: their characterizatiom and synthesis during spermatogenesis." Develop.Growth,Differ.33. 9-17 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto,K.& S.Nenoto: "Absence of species specificity of germinal vesicle factor required for the cytoplasmic cycles during meiotic division." Develop.Growth,Differ.32. 249-254 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tachibana,K.,M.Ishiura,T. Uchida & T.Kishimoto: "Starfish egg mRNA responsible for reinitiation of meiotic maturation encodes cyclin." Develop.Biol.140. 241-252 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takagi,T.,I,Mabuchi,H.Hosoya,K.Furuhashi & S.Hatano: "Primary structure of profilins from two species of Echinoidea and (Physarum polycepharum.)/ー" Eur.J.Biochem.192. 777-781 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai,T.& A.Yomota: "Cleavage plane determination in amphibian eggs." Ann.N.Y.Acad.Sci.582. 40-49 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 岸本 健雄: "卵の細胞周期を調節するしくみ (pp.23〜36)“発生・分化の遺伝子的脊景"(江口・鈴木・名取・(編))" 東京大学出版会, 288 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 片桐 千明: "精子核の凝縮と膨潤 (分担執筆)“精子学"(毛利秀雄監修" 東京大学出版会, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yokata, T., K. Takamune & Ch. Katagiri: "Nuclear basic proteins of Xenopus Iaevis sperm : their characterization and synthesis during spermatogenesis." Develop. Growth, Differ.33. 9-17 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Moriya, M. & Ch. Katagiri: "Immunoelectron microscopic localization of spermspecific nuclear basic proteins during spermatogenesis in amuran amphibians." Develop. Growth, Differ.33. 19-27 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, K. & S. Nemoto: "Absence of species specificity of germinal vesicle factor required for the cytoplasmic cycle during meiotic division." Develop. Growth, Differ.32. 249-254 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoneda, M. & S. Nemoto: "Occurrence of partial nuclei in eggs of the sand dollar, Clypeaster japonicus." Biol. Bull.179. 183-185 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hashimoto, N., S. Iwashita, Y. Shoji-Kasai, T. Kishimoto & K. Imahori: "Thiol protease inhibitor, E-64-d, Prevents spindle formation during mouse oocyte maturation." Develop. Growth, Differ.32. 179-203 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tachibana, K., M. Ishiura, T. Uchida & T. Kishimoto: "Starfish egg mRNA responsible for reinitiation of meiotic maturation encodes cyclin." Develop. Biol.140. 241-252 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mabuchi, I. & H. Takano-Ohmuro: "Effects of inhibitors of myosin light chain kinase and other protein kinases on the first cell division of sea urchin eggs." Develop. Growth, Differ.32. 549-556 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takagi, T., I. Mabuchi, H. Hosoya, K. Furuhashi & S. Hatano: "Primary structure of profilins from two species of Echinoidea and Physarum polycephalum." Eur. J. Biochem.192. 777-781 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai, T. & K. Higuchi: "Cytostatic effect of the cytoplasm of mature oocytes in the newt, Cynops pyrrhogaster" Zool. Sci.6. 709-714 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai, T. & A. Yomota: "Cleavage plane determination in amphibian eggs." Ann. N. Y. Acad. Sci.582. 40-49 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yokota,T.,K.Takamune & Ch.Katagiri: "Nuclear basic proteins of <Xenopus laevis>___ー sperm:their characterization and synthesis during spermatogenesis." Develop.Growth,Didder.33. 9-17 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Oka,M.T.,T.Arai & Y.Hamaguchi: "Heterogeneity of microtubules in dividing sea urchin eggs revealed by immunofluorescence microscopy:spindle microtubules are composed of tubulin isotypes different from those of astral microtubules." Cell Motil.Cytoskeleton. 16. 239-250 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tachibana,K.,M.Ishiura,T.Uchida & T.Kishimoto: "Starfish egg mRNA responsible for reinitiation of meiotic maturation encodes cyclin." Develop.Biol.140. 241-252 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mabuchi,I.& H.Takano Ohmuro: "Effects of inhibitors of myosin light chain kinase and other protein kinases on the first cell division of sea urchin eggs." Develop.Growth,Differ.32. 549-556 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sawai,T.& A.Yomota: "Cleavage plane determination in amphibian eggs." Ann.New York Acad.Sci.582. 40-49 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nomura,A.,Y.K.Maruyama & M.Yoneda: "Initiation of DNA replication cycle in fertilized eggs of the starfish,<Asterina>___ー <pectinifera>___ー." Develop.Biol.143. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamamoto,K.& S.Nemoto: "Absence of species-specificity of germinal vesicle factor requiring for the cytoplasmic cycle during meiotic division." Develop.Growth,Differ.32. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shilling,F.,L.A.Jaffe & T.Kishimoto: "Pertussis toxin inhibits l-methyladenine-induced maturation in starfish oocytes." Develop.Biol.133. 605-608 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohsumi,K.& Ch.Katagiri: "Dependence on germinal vesicle materials of the cyclic changes of nuclear behavior and cytoplasmic rigidity in activated frog eggs." Develop.Growth,differ.32. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shimizu,T.: "Polar body formation in Tubifex eggs." Ann.N.Y.Acad.Sci. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shinagawa,A.,Y.Yoshimoto& Y.Hiramoto: "Nuclear involvement in localization of the initiation site of surface contraction waves in Xenopus eggs." Develop.Growth,Differ.,. 31. 249-255 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sutoh,K. & I.Mabuchi: "End-label fingerprintings show that an N-terminal segment of depactin participates in interaction with action." Biochemistry. 28. 102-106 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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