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真核細胞における鞭毛・繊毛運動の分子機構の解明

Research Project

Project/Area Number 01304006
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionThe University of Tokyo

Principal Investigator

森沢 正昭 (森沢 正明)  東京大学, 理学部, 教授 (40013594)

Co-Investigator(Kenkyū-buntansha) 馬場 昭次  お茶の水女子大学, 理学部, 助教授 (80011691)
林 博司  名古屋大学, 理学部, 教授 (00022608)
渡辺 良雄  筑波大学, 生物科学系, 教授 (00015918)
高橋 景一  東京大学, 理学部, 教授 (40011481)
毛利 秀雄  東京大学, 教養学部, 教授 (70012268)
中村 健一  広島女子大学, 家政学部, 教授 (50094694)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1989: ¥10,000,000 (Direct Cost: ¥10,000,000)
Keywords微小管滑り運動 / 鞭毛・繊毛運動 / 精子運動開始 / 遺伝子クロ-ニング / チュ-ブリン / ダイニン / Ca^<2+>結合タンパク / プロテインキナ-ゼ / 鞭毛繊毛運動 / 滑り運動 / カルシウム / リン酸化反応 / コンホメ-ション変化 / 突然変異
Research Abstract

滑り運動の分子機構に関して高橋は独自に開発した装置で、高Ca^<2+>がウニ精子鞭毛の特定の滑りパタ-ンを高頻度で起すことを示し、上村は10^<ー9>mの精度でウニ精子鞭毛の微小管滑り運動を解析することに成功し、滑りがチュ-ブリンのサイズを反映した振幅で起ることを発見した。神谷はダイニン標品上を微小管を滑らせる実験系を開発し、ダイニンは異なる運動性を起す複数部分からなることを示し、豊島は同様の系で、ダイニンATPaseはキネシン、ミオシンのATPaseに比べ基質特異性が高いこと、加水分解と運動の速度の相関は低いことを示した。毛利はトリプシン分解法でウニの外腕ダイニンβ鎖のATP分解に伴うconformation変化とα鎖の相互作用が滑り運動に重要であることを示し、中村は微小管と微小管結合タンパクの給合を解析し、チュ-ブリンサブユニット間の数個の架橋様式の存在を示した。繊毛運動に関して馬場は、ウニ幼生繊毛に対する神経伝達物質の作用を調べ周期運動のONーOFFと対称性を調節している因子について示し、中岡はゾウリムシで細胞膜の電流変化が繊毛打頻度を調節している事を明らかにした。野口はゾウリムシ繊毛打の逆転がCa^<2+>の膜での流入流出により調節されていることを示した。精子運動開始の分子機構に関し、林はその中核部分がAキナ-ゼ、チロシンキナ-ゼ、15Kタンパクの複合体からなることを示し、又15Kタンパクの抗体を作製した。森沢は海産魚精子の運動開始には細胞内Ca^<2+>、細胞内pHが重要な役割を果していることを明らかにし、奥野は海産無脊椎動物フジツボでCa^<2+>の関与について研究を行った。渡辺はテトラヒメナのカルモジュリンファミリ-に属する3種のCa^<2+>給合タンパクを発見し、更にこれらの遺伝子のクロ-ニングとシ-クエンシングに成功した。又、これらの遺伝子のアンチセンスmRNAを調製し、微量注射法で、これらの結合タンパクの繊毛運動における機能について検討中である。

Report

(2 results)
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (91 results)

All Other

All Publications (91 results)

  • [Publications] Morisawa,M.,Inoda T.& Oda S.: "Reguration of sperm motility by osmotic pressure" Mechanism of Fertilization:Plants to Humans (B.Dale ed.) SpringerーVerlag. H45. 143-154 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Morisawa M.& Morisawa S.: "Acquisition and initiation of sperm motility" Controls of Sperm Motility:Biological and Clinical Aspects (C.Gagnom ed) CRC Press. 137-151 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Morisawa M.,Oda S.& Inoda T.: "Initiation of sperm motility by osmolality and calcium in teleost and amphibia" Proceedings of the VIth International Congress on Spermatology:Comparative Spermatology (Siena Italy).

    • Related Report
      1990 Annual Research Report
  • [Publications] Morisawa S.,Ishida K.& Morisawa M.: "The role of extracellular pH on the acquisition of sperm motility in chum salmon" Proceedings of the VIth International Congress on Spermatology:Comparative Spermatology (Siena Italy).

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Ogawa,E.Yokota,S.Wada,M.Okuno & Y.Nakajima: "The outer arm dynein αーheavy chains of sea urchin sperm flagella and emblionic cilia are different." Cell Motility and Cytoskeleton. 16. 58-67 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Shimizu,M.Okuno,S.P.MarcheseーRagona & K.A.Johnson: "Phosphorothioate analogs of ATP as the substrates of dynein and ciliary or flagellar movement." Europian Journal of Biochemistry. 191. 543-550 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inaba,Kazuo,Ono,Michio & Mohri,Hideo: "Conformational changes of the β chain of the outerーarm dynein from sea urchin sperm flagella coupled with ATP hydrolysis." Journal of Biochemistry. 108. 663-668 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mohri,Hideo,Fujiwara,Akiko,Daumae,Masashi & Mohri,Hideo: "Respiration and motility in starfish spermatozoa at various times in the breeding season." Development,Growth & Differentiation. 32. 375-381 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 稲葉 一男・毛利 秀雄: "鞭毛・繊毛運動の分子機構" 蛋白質 核酸 酵素. 34. 1505-1512 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inaba,Kazuo,Okuno,Makoto & Mohri,Hideo: "Anthraniloyl ATP,a fluorescent analog of ATP,as a substrate for dynein ATPase and flagellar motility." Archives of Biochemistry and Biophysics. 274. 209-215 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inaba,Kazuo & Mohri,Hideo: "Two states of the conformation of 21S outer arm dynein coupled with ATP hydrolysis." Journal of Biochemistry. 106. 349-354 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inaba,Kazuo & Mohri,Hideo: "Dynamic conformational changes of 21 S dynein ATPase coupled with ATP hydrolysis revealed by proteolytic digestion." The Journal of Biological Chemistry. 264. 8384-8388 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamada,A.,N.Ishii,T.Shimmen and K.Takahashi: "MgーATP activity and motility of native thick filaments isolated from the anterior byssus retractor muscle of Mytilus edulis." J.Muscle Res.Cell Motility. 10. 124-134 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Abe,O.,T.Yamada,K.Takahashi and H.Sugi: "Initiation of active force development in a molluscan smooth muscle by laser photolysis of caged ATP." Proc.Japan Academy Ser.B. 65. 165-168 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Katada,J.,C.Shingyoji and K.Takahashi: "Effects of detergents used to demembranate the seaーurchin spermatozoa on the reactivated flagellar movement,with special reference to the rotatability of the plane of flagellar beat." Cell Struct.Funct.14. 751-758 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshimura,K.and K.Takahashi: "Partial extraction of inner dynein arms from an outer armーless mutant of Chlamydomonas reinhadtii." Cell Struct.Funct.14. 741-749 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Eshel,D.,C.Shingyoji,K.Yoshimura,B.H.Gibbons,I.R.Gibbons and K.Takahashi: "Transient behavior of sea urchin sperm flagella following an abrupt change in beat frequency." J.exp.Biol.15. 441-451 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamada,A.,N.Ishii and K.Takahashi: "Direction and speed of actin filaments moving along thick filaments isolated from molluscan smooth muscle." J.Biochem.108. 341-343 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shingyoji,C.,I.R.Gibbons,A.Murakami and K.Takahashi: "Effects of imposed head vibration on the stability and waveform of flagellar beating in sea urchin spermatozoa." J.exp.Biol.,in press.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shingyoji,C.,J.Katada,K.Takahashi and I.R.Gibbons: "Rotating the plane of imposed vibration can rotate the plane of flagellar beating in sea urchin sperm without twisting the axoneme." J.Cell Sci.,in press.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takahashi,K.,C.Shingyoji,J.Katada,D.Eshel and I.R.Gibbons: "Polarity in spontaneous unwinding after prior rotation of the flagellar beat plane in sea urchin spermatozoa." J.Cell Sci.,in press.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ishii,N.,F.Mitsumori and K.Takahashi: "Changes in sarcoplasmic metabolite concentrations and pH associated with the catch contraction and relaxation of the anterior byssus retractor muscle of Mytilus edulis measured by phosphorusー31 nuclear magnetic resonance." J.Muscle Res.Cell Motil.,12,in press.

    • Related Report
      1990 Annual Research Report
  • [Publications] Machemer,H.,S.MachemerーRo^^‥hnisch,R Bra^^‥ucker and K.Takahashi: "Gravikinesis in Paramecium:Theory and isolation of a physiological response to the natural gravity vector." J.Comp.Physiol.,in press.

    • Related Report
      1990 Annual Research Report
  • [Publications] Ishii,N.,F.Mitsumori,K.Takahashi,A.W.M.Simpson and C.C.Ashley: "Intracellular metabolite and free calcium concentrations during the‘catch'contracation and relaxation in a molluscan smooth muscle." Muscle Energetics(eds R.Paul,G.Elzinga and K.Yamada),Alan R.Liss,NY.463-464 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hayashi H.,Takiguchi K.& HigashiーFujime S.: "Measurement of ATPase activity of immobilized myosin heads" J.Biochem.105. 875-877 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takiguchi K.,Hayashi H Kurimoto E.& HigashiーFujime S.: "In vitro motility of skeletal muscle myosin and its proteolytic fragments" J.Biochem.107. 671-679 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬場 昭次(Ooya et al.): "Swimming behavior of <Paramecium>___ー in the gravity fields:Analysis using gravity as an experimental variable." Proc.6th Space Util.Symp.6. 91-95 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬場 昭次(Ooya et al.): "Analysis of swimming behavior of <Paramecium>___ー under hypergravity conditions." Biol.Sci.Space. 3. 320-321 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬場 昭次: "Threeーdimensional recording and measurement of swimming paths of microーorganisms with two synchronized monochrome cameras." Rev.Sci.Instrum.1-2 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬場 昭次(Mogami et al.): "Five different states of ciliary activity in the epaulette of echinoplutei." J.Exp.Biol.155. 1-11 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬場 昭次: "Discrete nature of flagellar bending detected by digital image analysis." Biomathematics. 1-10 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬場 昭次: "Highーresolution analysis of flagellar bends." Comparative Spermatology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takemasa,T.,Takagi,T.,Kobayashi,T.,Konishi,K.,& Watanabe,Y.: "The third calmodulin family protein in <Tetrahymena>___ー:Cloning of the gene for <Tetrahymena>___ー calciumーbinding protein of 23kDa (TCBPー23)" Journal of Biological Chemistry. 265. 2514-2517 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.,Ohba,H.,Hirono,M.,& Yasuda,T.: "Analysis of furrow formation and furrowing during cell division in <Tetrahymena>___ー using cellーdivisionーarrest mutants" Annals of The New York Academy of Sciences. 582. 166-178 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Edamatsu,M.,Hirono,M.,Watanabe,Y.: "Purification and characterization of <Tetrahymena>___ー profilin" Biophysica Biochimica Research Communication. 170. 957-962 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.,Hirono,M.,Takemasa,T.,& Numata,O.: "Protozoan genes:As viewed from <Tetrahymena>___ー genes" Japanese Journal of Protozoology. 23. 1-11 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirono,M.,Tanaka,R.,& Watanabe,Y.: "<Tetrahymena>___ー actin:Copolymerization with skeletal muscle actin and interactions with muscle actinーbinding proteins" Journal of Biochemistry. 107. 32-36 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Numata,O.,Tomiyoshi,T.Kurasawa,Y.,Fu,Zhen xing,Takahashi,M.,Kosaka,T.Chiba,J.,& watanabe,Y.: "Antibodies against <Tetrahymena>___ー 14ーnm filament forming protein recogniz replication bund in <Euplotes>___ー" Experimental Cell Research. 193. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kagami,O.and Kamiya,R.: "Strikingly low ATPase in flagellar axonemes of a Chlamydomonas mutant missing outer dynein arms." European Journal of Biochemistry. 187. 441-446 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kagami,O.,Takada,S.and Kamiya R.: "Microtubule translocation caused by three subspecies of innerーarm dynein from Chlamydomonas flagella." FEBS Letters. 264. 179-182 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kamiya,R.,Kurimoto,E.and Muto,E.: "Two types of flagellar mutants missing different components of innerーarm dynein." Journal of Cell Biology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakamura,K.,Masuyama,E.: "Characterization of an Axonemal Protein from Tetrahymena cilia." Bull.Hiroshima Women's Univ.25. 21-28 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakamura,K.,Masuyama,E.Wada,S.,and Okuno,M.: "Application of Stainsーall Staining to the Analysis of Axonemal tubulins." J.Biochem.Biophys.Methods. 21. 237-245 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakamura,K.,Furukohri,T.Shikama,K.: "A Computer Display of the Free Energy Changes of ATP Hydrolysis." Biochem.Education. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nakaoka & H.Machemer: "Effects of cyclic nucleotides and intracellular Ca on voltageーactivated ciliary beating in <Paramecium>___ー" Journal of Comparative Physiology. 166. 401-406 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Inoue & Y.Nakaoka: "Coldーsensitive responses in the <Paramecium>___ー membrane" Cell Structure and Function. 15. 107-112 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Tokioka,K.Matsuoka,Y.Nakaoka & Y.Kito: "Extraction of retinal from <Paramecium>___ー <bursaria>___ー" Photochemistry and Photobiology. 53. 149-151 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nakaoka,R.Tokioka,T.Shinozawa,J.Fujita & J.Usukura: "Photoreception of <Paramecium>___ー cilia:Localization of photosensitivity and binding with antiーfrog rhodopsin IgG" Journal of Cell Science. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shinji Kamimura and Ritsu Kamiya: "Highーfrequency nanometerーscale vibration in“quiescent"flagellar axonemes." Nature. 340. 476-478 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Noguchi,Y.Nakamura and K.Okamoto: "Control of the ciliary orientation on the ciliated sheets of <Paramecium>___ーーーDifferential distribution of sensitivity to cyclic nucleotides." Submitted to Cell Motil.Cytoskeleton.

    • Related Report
      1990 Annual Research Report
  • [Publications] Toyoshima,Y.Y.,Kron,S.J.& Spudich,J.A.: "The myosin step size:Measurement of the unit displacement per ATP hydrolysed in an in vitro assay." Proc.Natl.Acad.Sci.87. 7130-7134 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Schwyter,D.H.,Kron,S.J.,Toyoshima,Y.Y.,Spudich,J.A.& Reisler,E.: "Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin." J.Cell Biol.111. 465-470 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 豊島 陽子,毛利 秀雄: "微小管関連モ-タ-タンパク質" 比較生理生化学. 7. 71-79 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toyoshima,Y.Y.: "Flexibility in actinーmyosin motility system revealed by in vitro motility assay." Adv.Biophys.(1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toyoshima,Y.Y.: "Bidirectional movement of actin filaments along tracks of heavy meromyosin and native thick filaments." J.Cell Sci.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimizu,T.,Furusawa,K.,Ohashi S.,Toyoshima,Y.Y.,Okuno,M.,Malik,F.& Vale R.D.: "Nucleotide specificity of the enzymatic and motile activities of dynein,kinesin and heavy meromyosin." J.Cell Biol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.,HiranoーOhnishi,J.,& Takemasa,T.: "Calciumーbinding proteins and ciliary movement regulation in <Tetrahymena>___ー,Chaptor 19 in Calcium as an Intracellular Messenger in Eucaryotic Microbes,edited by D.h.O'Day" Ammerican Society for Microbiology Washington,D.C., 346-361 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takemasa,T.,Takagi,T.,& Watanabe,Y.: "Calciumーbinding protein from <Tetrahymena>___ー,In Novel CalciumーBinding Proteins.Fundamentals and Clinical Implications,edited by C.Heizmann" Springer Press, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kron,S.J.,Toyoshima,Y.Y.,Uyeda,T.Q.P.& Spudich,J.A.: "Assays for actin sliding movement over myosin coated surfaces.In“Methods in Enzymology"" Academic Press, (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Morisawa M.: "Regulation of sperm motility by osmotiy pressure" In"Mechanism of Fertilization:Plants to Human"B.Dale ed.NATO ASI series on cell Biology,Springer-Verlag,Berlin Heiderberh,New York.

    • Related Report
      1989 Annual Research Report
  • [Publications] Morisawa M.: "Acquisition and initiation of sperm motility" In"Control of Sperm Motility"C.Gagnon ed.CRC Press,Inc.Boca Raton.

    • Related Report
      1989 Annual Research Report
  • [Publications] Okuno M.: "Effects of calcium on the motility of rainbow trout sperm flagella demembranated with Triton X-100" Cell Motility and Cytoskeleton. 14. 194-200 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inaba K.: "dynamic conformational changes of 21 s dynein ATPase coupled with ATP hydrolysis revealed by proteolytic digestion" The Journal of Biological Chemistry. 264. 8384-8388 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inaba K.: "Two states of the comformation of 21 s outer arm dynein coupled with ATP hydrolysis" The Journal of Biological Chemistry(TokYo). 106. 349-354 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inaba K.: "Comformational changes of the chain of the outer arm dynein from sea urchin sperm flagella coupled with ATP hydrolysis" The Journal of Biological Chemistry.

    • Related Report
      1989 Annual Research Report
  • [Publications] Inaba K.: "Anthraniloyl ATP,a fluorescent analog of ATp,as a substrate for dynein ATpase and flagellar motility" Archives of Biochemistry and Biophysics. 274. 209-215 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshimura K.: "Partial extraction of inner dynein arms from an outer arm-less mutant of Chlamydomonas reinbardtii" Cell Structure and Function. 14. 741-749 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Katada J.: "Effects of detergents used to demembranate the sea-urchin spermatozoa on teh reactivated flagellar movement.with special reference to the totatability of the plane fo flagellar beat" Cell Structure and Function. 14. 751-758 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Abe O: "Initiation of ctie force development in a molluscan smooth muscle by laser photolysis of caged ATP" proceedings of Japan Academy,Ser B. 65. 165-168 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Eshel D.: "Transient behaviow of sea urchin sperm flagella following" Journal of Experimental Biology. in press.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hayashi H.: "Measurement of ATPase activity of immobilized myosin heads" Journal of Biochemistry(Tokyo). 105. 875-877 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kamimura S.: "High-frequency nanometre-scale vibration in 'quiescent'flagellar axoneme" Nature. 340. 476-478 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Baba S.: "Discrete nature of flagellar bending detected by digital image analysis." Biomathematics. in press.

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakamura K.: "Characterization of an axonemal protein from Tetrahymena cilia" Bull.Hiroshima Women s University. 25. 21-28 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakaoka Y.: "Localization of photoreceptor in Paramecium bursaria" journal of Comparative Physiology A. 165. 637-643 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakaoka Y.: "Effects of cyclic nucleotides and intracellular Ca^<2+> on voltage activatid ciliary beating in paramecium" journal of Comparative Physiology A. in press.

    • Related Report
      1989 Annual Research Report
  • [Publications] Oka T.: "Inactivation and activation of inward current during adaptation to potassium ions in Paramecium caudatum" Cell Structure and Function. 14. 209-216 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakajima K.: "Circadian changes of photosensitivity in Paramecium bursaria" Journal of Experimental Biology. 144. 43-51 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakakibara H.: "Functional recombinaton of outer dynein arms with outer arm-missing flagellar axonemes of a Chlamydomonas mutant" Journal of Cell Science. 92. 77-83 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kamiya R.: "Strategies for the selection of Chlamydomonas dynein mutant" Methods in Enzymology. in press.

    • Related Report
      1989 Annual Research Report
  • [Publications] Kagami O.: "Strikingly low ATPase in flagellar axonemes of a Chlamidomonas mutant missing outer dynein arms" European Journal of Biochemistry. in press.

    • Related Report
      1989 Annual Research Report
  • [Publications] 神谷律: "鞭毛運動とダイニン内腕・外腕" 生物物理. 29. 231-236 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Vale D.: "In vitro motility assays for kinesin and dynein" In"Cell Movement"(Alan R,Liss,Inc.). 2. 287-294 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Vale R.: "Microtubule translocation properties of intact and proteolytically digested dyneins from Tetrahymena cilia" Journal of Cell Biology. 108. 2327-2334 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Toyoshima Y.: "Bidirectional movement of actin filaments along tracks of myosin heads" Nature. 341. 154-156 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kron S.J.: "Assays for actin sliding movement over myoshin coated surfaces" Methods in Enzymology. in press.

    • Related Report
      1989 Annual Research Report
  • [Publications] 豊島陽子: "アクチンシオミンの運動再構成系" 蛋白質核酸酵素. 34. 1480-1486 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 稲葉一男・毛利秀雄: "鞭毛・繊毛運動の分子機構蛋白質、核酸酵素「細胞骨格の機能」(酒井彦一編)" 共立出版, 1989

    • Related Report
      1989 Annual Research Report
  • [Publications] 上村慎治: "生物理実験技術『光学顕微鏡によるナノメ-タ-精度での運動解析』" 吉岡書店, 10 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 上村慎治: "細胞・細胞運動の定量法" ニュ-サイエンス社, 10 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 上村慎治: "アトバンテストテクノロジ-生物工学編第13巻生体物理工学 第3章ナノメ-トル精度での顕微計測、顕微操作" 丸善株式会社出版事業部, 20 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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