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Structure of Starch Hydroly Patterns by Enzymes and Changes in Chemical and Physicochemical Properties Under Various Conditions

Research Project

Project/Area Number 01304016
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionFukuyama University, Faculty of Engineering

Principal Investigator

YAMAMOTO Takehiko  Fukuyama Univ. Faculty of Engineering Prof., 工学部, 教授 (50046710)

Co-Investigator(Kenkyū-buntansha) YAMANE Kunio  Tsukuba Univ. Faculty of Bioscience Prof., 生物科学系, 教授 (20013336)
MINAMIURA Noushi  Osaka City Univ. Faculty of science Prof., 理学部, 教授 (20047129)
HIZUKURI Susumu  Kagoshima Univ. Faculty of Agriculture Prof., 農学部, 教授 (90041590)
KOMAKI Toshiaki  Fukuyama Univ. Faculty of Engineering Prof., 工学部, 教授 (00205501)
FUWA Hidetsugu  Fukuyama Univ. Faculty of Engineering Prof., 工学部, 教授 (00046765)
北畑 寿美雄  大阪市立工業研究所, 研究主任
谷口 肇  農水省, 食糧総合研究所, 室長
溝上 恭平  福山大学, 工学部, 講師 (00200030)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 1990: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1989: ¥5,900,000 (Direct Cost: ¥5,900,000)
Keywordsstarch / amylopectin / amylase(alpha, beta, gluco-) / amylase gene / cyclodextrin / maltose / glucose / アミラ-ゼ(αー,βー,グルコ-) / エネルギ-源としての澱粉 / グルコアミラ-ゼ / 生澱粉の被分解性 / 湿式加熱処理澱粉 / 耐熱性β-アミラ-ゼ / 高収率、高純度のマルト-ス / α-アミラ-ゼ遺伝子のクロ-ニング / サイクロデチストリン合成酵素
Research Abstract

Starch is the most universal energy source not only for human, but also for various other living organisms. Also, starch is important as an industrial material. It is renewable resource and its production is easy. Therefore, the more industrial application of starch may be possible. The present research was started to make more clearly the structure of starch and starch granule, especially the hydrolysis pattern by enzymes and changes in chemical and physicochemical properties of starch under various conditions.
The results obtained were : Corn-and rice starch granules were shown to be attacked by glucoamylase to make many pin holes on certain areas of the granule surface, indicating that the starch granule is not uniform structurally. The amylopectin of wheat starch was found to be consisted of two A chains per B chain in average, but the longer B chain was branched with the more A chains this fact also indicated that the structure of starch is not uniformity. Several new amylases were discovered in this project research, such as thermal stable beta-amylase of bacterial origin, a new enzyme which has both activities of alpha-amylase and pullulanase, etc. Several methods of processing starch and cyclodextrins were investigated. Under certain conditions starch was converted to glucose almost completely by alpha-amylase alone, the addition of glucose to cyclodextrin to be a branch greatly changed the properties of the cyclodextrin, and so on.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] T.Yamamoto,I.Miyahara,S.Yamamoto,K.Fujita and K.Mizokami: "αーAmylase of Rhiz.niveus: It's isolation and some enzymatic propeities" Denpun Kagaku. 37. 129-136 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Hizukuri and Y.Maehara: "Five structure of wheat amylopectin:The mode of A to B chain binding" Carbohydrate Res.206. 145-159 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hidetsugu Fuwa: "Characterization of Amylopectin and Granular Starch Studies in Japan since Professor Ziro Nikuni" International Symposium on Cereal and Other Plant Carbchydrates(Kagoshima,Japan). 1-2 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小巻 利章: "澱粉の湿熱処理による物性の変化について" 澱粉科学. 36. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.C.Ann,M.Iizuka,T.Yamamoto and N.Minamiura: "Active monomer of sweet potato βーamylase:Stabilization and improved preparation method using αーcyclodextrin" J.Ferment.Bioeng.72. 75-79 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Itoh,K.Kanoh,K.Nakamura,K.Takase and K.Yamane: "Artificial insertion of peptides between signal peptide and mature protein:Effect on secretion and processing of hybrid thermostable αーamylase in B.subtilis and E.coli cells" J.Gen.Microbiol.136. 1551-1558 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 大西 正健: "実験でみる生化学" 共立出版社, 290 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Yamamoto, I. Miyahara, S. Yamamoto, K. Fujita, and K. Mizokami: "alpha-Amylase of Rhiz. niveus : It's isolation and some enzymatic properties" Denpun Kagaku. 37. 129-136 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Hizukuri and Y. Maehara: "Five structure of wheat amylopectin : The mode of A to B chain binding" Carbohydrate Res.206. 145-159 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hidetsugu Fuwa: "Characterization of Amylopectin and Granular Starch Studies in Japan since Professor Ziro Nikuni" International Symposium on Cereal and Other Plant Carbohydrates(Kagoshima, Japan). 1-2 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. C. Ann, M. Iizuka, T. Yamamoto and N. Minamiura: "Active monomer of sweet potato beta-amylase : Stabilization and improved preparation method using alpha-cyclodextrin" J. Ferment. Bioeng.72. 75-79 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Itoh, K. Kanoh, K. Nakamura, K. Takase and K. Yamane: "Artificial insertion of peptides between signal peptide and mature protein : Effect on secretion and processing of hybrid thermostable alpha-amylase in B. subtilis and E. coli cells" J. Gen. Microbiol.136. 1551-1558 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masataka Ohnishi: "Kyoritsu Press Corp. (Tokyo)" Book Biochemistry through experiments. 290 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Yamamoto,I.Miyahara,S.Yamamoto,K.Fujita and K.Mizokami: "αーAmylase of <Rhiz>___ー・<niveus>___ー: It's isolation and some enzymatic propeities" Denpun Kagaku. 37. 129-136 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Hizukuri and Y.Maehara: "Five structure of wheat amylopectin:The mode of A to B chain binding" Carbohydrate Res.206. 145-159 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hidetsugu Fuwa: "Characterization of Amylopectin and Granular Starch Studies in Japan since Professor Ziro Nikuni" International Symposium on Cereal and Other Plant Carbchydrates(Kagoshima,Japan). 1-2 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小巻 利章: "澱粉の湿熱処理による物性の変化について" 澱粉科学. 36. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.C.Ann,M.Iizuka,T.Yamamoto and N.Minamiura: "Active monomer of sweet potato βーamylase: Stabilization and improved preparation method using αーcyclodextrin" J.Ferment.Bioeng.72. 75-79 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Itoh,K.Kanoh,K.Nakamura,K.Takase and K.Yamane: "Artificial insertion of peptides between signal peptide and mature protein:Effect on secretion and processing of hybrid thermostable αーamylase in B__ー.<subtilis>___ー and E__ー.<coli>___ー cells" J.Gen.Microbiol.136. 1551-1558 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大西 正健: "実験でみる生化学" 共立出版社, 290 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 山本武彦、宮原泉、山本学、藤田佳平衛、溝上恭平: "α-Amylase of Rhizoplis niveus:Its Isolation and Some Enzymatic Prcperties" 日本澱粉科学会誌. 37. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Hizukuri and Y.Maehara: "The Branching Frequency of Wheat Starch Amylopectin" 198th ACS Naticnal Meeting,Miami Beach,Florida. CARB. 61-66 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Sata,M.Umeda,C.H.Kim,H.Taniguchi: "Amylase-Pullulanase Enzyme Produced by B.cirulans" Biochimica et Biophysica Acta. 991. 388-394 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Kitahata,H.Ishikawa,T.Miyata and O.Tanaka: "Production of Rubuscside Derivatives by Transgalactosylation of various β-galactosidaces" Agricultutal and Biological Chemistry. 53. 2923-2928 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Minamiura,K.Ito,L.Lan-Fen,P.Chaimanee,R.Vejaratpimol,P.Ruenwongas and K.Kataoka: "Discriminative Assay Method of Human Pancreatic and Salivary α-Amylases Using Monoclonal Antibody" Microbial Utilization of Renewdble Resources. 6. 49-55 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Ohnishi and K.Hiromi: "Binding of Maltose to Rhizopus niveus glucoamylase in the pH Range where the Carboxyl Groups Are Ionized" Carbohydrate Reseaches. 195. 138-144 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Yamamoto,S.Kitahata,K.Hiromi,M.Ohnishi,N.Minamiura,R.Shinke,S.Okada and T.Komaki: "Handbcok of Amylases and Related Enzymes" Pergamon Press pic,Headington Hill Hall,Oxford Ox3,OBW,England, 274 (1988)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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