• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and Cell Biology of a Novel Multicatalytic Proteinase Complex

Research Project

Project/Area Number 01304029
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionThe University of Tokushima

Principal Investigator

ICHIHARA Akira  Institute for Enzyme Research University of Tokushima, Professor, 酵素科学研究センター, 教授 (40035374)

Co-Investigator(Kenkyū-buntansha) YOKOSAWA Hideyoshi  School of Pharmacy, Hokkaido University, Professor, 薬学部, 教授 (90012765)
SASAKI Makoto  School of Medicine, Nagoya City University, Professor, 医学部, 教授 (10080003)
ISHIURA Shoichi  Division of Neuromuscular Research National Institute of Neuroscience Division h, 室長 (10158743)
IKAI Atsushi  Faculty of Science, Tokyo Inst. of Technology, professor, 理学部, 教授 (50011713)
TANAKA Keiji  Institute for Enzyme Research University of Tokushima, Research assistant, 酵素科学研究センター, 助手 (10108871)
石川 勝利  静岡大学, 理学部, 教授 (00008608)
村地 孝  京都大学, 医学部, 教授 (10089104)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1990: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1989: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsProteasomes / Multicatalytic protease / Structural analysis / Multisubunit complex / High molecular weight protease / 分子遺伝学 / 多機能プロテア-ゼ複合体 / 細胞内プロテア-ゼ / 20S環状粒子 / cDNAクロ-ニング / 発生と分化
Research Abstract

Multicatalytic proteinase complex (Proteasomes) is a large assembly of many protein components with various protease activities. In 1990 we carried out a co-operative research to investigate structure and functions of proteasomes.
1. Structural analysis --- cDNAs of 15 components of the proteasomes from yeast, rat and human were cloned and sequenced. These sequences showed no homology with any known proteins indicating that the proteasomes consist of subunits which are new protein family. This consideration is supported by the facts that homology among subunits from different animals was very high. Assembly of subunits to form a ring-shape particle can be observed by electron microscopy and similarity of shape among proteasomes of different animals suggests that structure of complex is important for the function.
2. Functions of proteasomes --- It was shown that proteasomes play important role in embryogenesis, particularly maturation and fertilization of egg. The expression of proteasome mRNA was very high in cancerous tissues of liver, kidney and blood cells. It was also found that proteasomes translocate etween cytosol and nucleus during cell growth. These results indicate that proteasomes relate closely with cell growth and differentiation. This notion was supported by experiments of gene disruption of yeast subunits. Yeast received disrupted genes became lethal.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Tsutomu Fujiwara: "Molecular cloning lf cDNA for proteasomes (multicatalytic proteinase complexes) from rat liver: primary structure of the largest component(C2)." Biochemistry. 28. 7332-7340 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiji Tanaka: "Molecular cloning of cDNA for proteasomes from rat liver: primary structure of component C3 with a possible tyrosine phosphorylation site" Biochemistry. 29. 3777-3785 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumunori Tokunaga: "The NH_2ーterminal residues oF rat liver proteasome (multicatalytic proteinase complex) subunits,C2,C3 and C8,are Nαーacetylated" FEBS Lett.263. 373-375 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiji Tanaka: "Proteasomes (multicatalytic proteinase complexes) in eukaryotic cells" Cell Struc.Func.15. 127-132 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsutomu fujiwara: "Proteasomes are essential for yeast proliferation: cDNA cloning and gene disruption of two major subunits" J.Biol.Chem.265. 16604-16613 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Atsushi Kumatori: "Abnormally high expression of proteasomes in human leukemic cells" Proc.Natl.Acad.Sci.U.S.A.87. 7671-7675 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomohiro Tamura: "Molecular cloning and sequence snalysis of cDNAs for five major subunits of human liver proteasomes (multiーcatalytyic proteinase complexes)" Biochim.Biophys.Acta. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Etsuko Orino: "ATPーdependent reversible association of proteasomes with multiple protein factors to form 26S complexes that degrade ubiquitinated proteins in human HLー60 cells" Proc.Natl.Acad.Sci.U.S.A.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A.Azuma: "High molecular weightーmulticatalytic proteinases in premature and mature oocytes of Rana pipens" Mol.Cell.Biochem.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shoichi Ishiura: "Addition of ATP increases the apparent molecular mass of the multicatalytic proteinae,ingensin" FEBS Lett.257. 123-126 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Sorimachi: "Molecular cloning of cDNA for subunits of rat multicatalytic proteinase" Eur.J.Biochem.193. 775-781 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Emori: "Molecular cloning and functional analysis of three subunits of yeast proteasome" Mol.Cell.Biol.11. 344-353 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] I.Ohkubo: "Human erhthrocyte multicatalytic proteinse.Activation and binding to sulfated galactoーand lactosylceramides" Biochem.Biophys.Res.Commun.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Kinoshita: "Induction of carp muscle multicatalytic proteinase activities by sodium dodecyl sulfate and heating" Comp.Biochem.Physiol.96B. 565-569 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Saitoh: "Sodium dodecyl sulfateーinduced conformational and enzymatic changes of multiーcatalytic proteinase" Biochem.Biophys.Res.Communi.162. 334-339 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Saitoh: "Comparative studies of proteasomes (multicatalytic proteinases) isolated from spermatozona and eggs of sea urchins." Comp.Biochem.Physiol.in press.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fufjiwara, T., Tanaka, K., Kumatori, A., Shin, S., Yoshimura, T., Ichihara, A., Tokunaga, F., Aurga, R., Iwanaga, S., Kakizuka, A., and Nakanishi, S.: "Molecular cloning of cDNA for proteasomes (multicatalytic proteinase complexes) from rat liver : primary structure of the largest component (C2)." Biochemistry. 28. 7332-7340 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanaka, K., Fujiwara, T., Kumatori, A., Shin, S., Yoshimura, T., Ichihara, A., Tokunaga, F., Aruga, R., Iwanaga, S., Kakizuka, A., and Nakanishi, S.: "Molecular cloning of cDNA for proteasomes from rat liver : primary structure of component C3 with a possible tyrosine phosphorylation site." Biochemistry. 29. 3777-3785 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tokunaga, F., Aruga, R., Iwanaga, K., Tanaka, S., Ichihara, A., Takao, T., and Shimonishi, Y.: "The NH_2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are Nalpha-acetylated." FEBS Lett.263. 373-375 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanaka, K., and Ichihara, A.: "Proteasomes (multicatalytic proteinase complexes) in eukaryotic cells." Cell Struc. Func. 15. 127-132 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujiwara, T., Tanaka, K., Orino, E., Yoshimura, T., Kumatori, A., Tamura, T., Chung, C., H. Nakai, T., Yamaguchi, K., Shin, S., Kakizuka, A., Nakanishi, S. and Ichihara, A.: "Proteasomes are essential for yeast proliferation : cDNA cloning and gene disruption of two major subunits." J. Biol. Chem.265. 16604-16613 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kumatori, A., Tanaka, K., Inamura, N., Sone, S., Ogura, T., Tachikawa, T., S. and Ichihara, A.: "Abnormally high expression of proteasomes in human leukemic cells." Proc. Natl. Acad. Sci. U. S. A.87. 7071-7075 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanaka, K., Yosdhimura, T., Tamura, T., Fujiwara, T., and Ichihara, A.: "Possible mechanism of nuclear translocation of proteasomes." FEBS Lett.271. 41-46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tamura, T., Lee, D. H., Osaka, F., Fujiwara, F., Shin, S., Chung, C. H., Tanaka, T. and Ichihara, A.: "Molecular cloning and sequence analysis of cDNAs for five major subunits of human liver proteasomes (multi-catalytyic proteinase complexes)." Biochim. Biophys. Acta. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Orino, E., Tanaka, K., Tamura, T., Sone, S., Ogura, T., and Ichihara, A.: "ATP-dependent reversible association of proteasomes with multiple protein factors to form 26S complexes that degrade ubiquitinated proteins in human HL-60 cells. Proc." Natl. Acad. Sci. U. S. A.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Azuma, A., Tokunoto, T. and Ishikawa, K.: "High molecular weight- multicatalytic proteinases in premature and mature oocytes of Rana pipens." Mol. Cell. Biochem.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ishiura, S., Tsukahara, T., and Sugita, H.: "Addition of ATP increases the apparent molecular mass of the multicatalytic proteinae, ingensin." FEBS Lett.,. 257. 123-126.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sorimachi, H., Tsukahara, H., Kawasaki, H., Ishiura, S., Emori, H., Sugita, H., and Suzuki, K: "Molecular cloning of cDNA for subunits of rat multicatalytic proteinase" Eur. J. Biochem.193. 775-781 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Emori, Y., Tsukahara, H., Kawasaki, H., Ishiura, S., Sugita, H., and Suzuki, K.: "Molecular cloning and functional analysis of three subunits of yeast proteasome" Mol. Cell. Biol.11. 344-353 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohkubo, I., Gasa, S., Namikawa, C., Makita, A., and Sasaki, M.: "Human erhthrocyte multicatalytic proteinse. Activation and binding to sulfated galacto- and lactosylceramides." Biochem. Biophys. Res. Commun.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kinoshita, M., Toyohara, H., and Shimizu, Y.: "Induction of carp muscle multicatalytic proteinase activities by sodium dodecyl sulfate and heating." Comp. Biochem. Physiol.96B. 565-569 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiji Tanaka: "Molecular cloning of cDNA for proteasomes from rat liver:primary structure of component C3 with a possible tyrosine phosphorylation site" Biochemistry. 29. 3777-3785 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fumunori Tokunaga: "The NH_2ーterminal residues of rat liver proteasome(multicatalytic proteinase complex)subunits,C2,C3 and C8,are Nαーacetylated" FEBS Lett.263. 373-375 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tsutomu Fujiwara: "Proteasomes are essential for yeast proliferation:cDNA cloning and gene disruption of two major subunits" J.Biol.Chem.265. 16604-16613 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Atsushi Kumatori: "Abnormally high expression of proteasomes in human leukemic cells" Proc.Natl.Acad.Sci.U.S.A.87. 7071-7075 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tomohiro Tamura: "Molecular cloning and sequence snalysis of cDNAs for five major subunits of human liver proteasomes(multiーcatalytyic proteinase complexes)" Biochim.Biophys.Acta. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Azuma: "High molecular weightーmulticatalytic proteinases in premature and mature oocytes of Rana pipens" Mol.Cell.Biochem.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Emori: "Molecular cloning and functional analysis of three subunits of yeast proteasome" Mol.Cell.Biol.11. 344-353 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Saitoh: "Sodium dodecyl sulfateーinduced conformational and enzymatic changes of multiーcatalytic proteinase" Biochem.Biophys.Res.Communi.162. 334-339 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Tanaka: "Molecular Cloning of cDNA for Proteasomes(Multicatalytic Proteinase Complexes)from Rat Liver:Primary Structure of Component C3 with Possible Tyrosine Phosphorylation Site." Biochemstry. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Fujiwara: "Molecular Cloning of cDNA for Proteasomes(Multicatalytic Proteinase Complexes)from Rat Liver:Primary Structure of the Large Component(C2)." Biochemistry. 28. 7332-7340 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] W.Matthews: "Involvement of the Proteasome in Various Degradative Processes in Mammalian Cells." Proc.Natl.Acad.Sci.USA. 86. 2597-2601 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Tanaka: "Direct Evidence for Nuclear and Cytoplasmic Colocation of Proteasomes(Multiprotease Complexes)in Liver." J.Cell.Physiol.139. 34-41 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Murachi: "Intracellular regulatory system involving calpain and calpastatin" Biochem.Int.18. 263-294 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ishiura,S.: "Putative N-terminal splitting enzyme of amyloid A4 peptide is the multi catalytic proteinase,ingensin,widely distributed in mammalian cells." FEBS Lett.257. 388-392 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tsukahara,T.: "RNA degrading activity is tightly associated with the multicatalytic proteinase,ingensin." FEBS Lett.255. 179-183 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Saitoh: "Purification and characterization of multicatalytic proteinase from eggs of the ascidian,Halocynthia roretzi." J.Biochem.105. 254-260 (1989)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi