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Molecular Genetic Analysis and Mechanism of Action of Bacterial Cytolysins

Research Project

Project/Area Number 01304033
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionOkayama University

Principal Investigator

SHINODA Sumio  Okayama University, Faculty of Pharmaceutcal Sciences, Professor, 薬学部, 教授 (50029782)

Co-Investigator(Kenkyū-buntansha) HONDA Takesh  Osaka University, Research Insititute for Microbial Diseases, Associate Professo, 微生物病研究所, 助教授 (60029808)
NAKAZAWA Teruko  Yamaguchi University, School of Medicine, Professor, 医学部, 教授 (40053053)
MIZUGUCHI Yasuo  Univercity of Occupational and Environmental Health, School of Medicine, Profess, 医学部, 教授 (10072919)
SAKURAI Jun  Tokushima Bunri University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (80029800)
NODA Masatoshi  Chiba University, School of Medicne, Professor, 医学部, 教授 (60164703)
加藤 巌  千葉大学, 医学部, 教授 (40012702)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1990: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1989: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsHemolysin / Cytolysin / Erythrocytes / Vibrio parahaemolyticus / ibrio vulnificus / Staphylococcus aureus / Clostridium perfringens / Pseudomonas cepatia
Research Abstract

This research projiects was organized to accumulate the information of property of various bacterial cytolysisns and to understand their exact role in infection. Shinoda purified Vibrio vulnificus hemolysin (VVH) and studied its property. Sensitivity of erythrocytes to VVH varied with the animal species because of difference of their ability to bind VVH. Some strains produced immunologically different VVH. Katoh and Noda studied the mechanism of cytolytic action of alpha-toxin and leukocidin of Staphylococcus aureus and found that their NAD-ribosylation activity was important to the lytic action. They also showed the exact role of S- and F-fragment of leukocidin. Sakurai found that active center for hemolytic activity and phospholipase of alpha-toxin of Clostridium perfrigens located on different sites of the toxin molecule. Furthermore, he showed change of phospholipid metabolism in action of the toxin on contraction of blood vessel smooth muscle. Honda studied structure and function of V. parahaemolyticus thermostable direct hemolysin (TDH). He isolated a mutant producing a TDH-related molecule which did non show hemolytic actibity and found that glycine residue at 90th was replaced with asparagine in the mutant hemolysin. Mizuguchi isolated a transformant which had gene encoding a hemolysin (delta-VPH) of V. parahaemolyticus. Delta-VPH was not produced by usual cultivation of V. parahaemolyticus. However, challenge of delta-VPH preparation to animal model suggested that the toxin was one of the pathogenic factor of the vibrio. Nakazawa found a new hemolysin of Pseudomonas cepatia The hemolysin was noon-proteinic and showed antimycotic activity. These results presented the important information for general understanding of the mechanism of action of bacterial cytolysins.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Honda,Takeshi: "A mutant hemolysin with lower biological activity produced by a mutant Vibrio Parahaemolyticus." FEMS Microbiol.Lett.61. 95-100 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kato,Iwao: "ADPーrbosylation of cell membrane proteins by staphylococal alphaーtoxin and leukocidin in rabbit erythrocytes and polymorphonuclear leukocytes." FEBS Lett.255. 59-62 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nakazawa,Teruko: "Cloning,sequencing,and transcriptional analysis of the recA gene of Pseudomonas cepatia." Gene. 94. 83-88 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakurai,Jun: "Dissociation of various biological activities of Clostridium perfringens alpha toxin by chemical modification." Toxicon. 27. 317-323 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi,Hatumi: "Cloning and characterization of a gene encoding a new thermoatable hemolysin from Vibrio parahaemolyticus." FEMS Microbiol. Lett.67. 339-346 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamanaka,Hiroyasu: "Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin." FEMS Microbiol. Lett.61. 251-256 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujii, Y. and Sakurai, J.: "Contraction of the rat isolated aorta caused by Clostridium perfringens alpha toxin (phospholipase C) ; evidence for the involvement of arachidonic acid metabolism." Br. J. Pharmacol.97. 119-124 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hoashi, K., Ogata, K., Taniguchi, H., Yamashiya, H., Tsuji, K., Mizuguchi, Y. and Ohtomo. N.: "Pathogenesis of Vibrio parahaemolyticus : Intraperitoneal and orgastric challenge experiments in mice." Microbiol. Immunol.,. 34. 355-366 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, T., Ni, Y. and Miwatani, T.: "Purification of a TDH-related hemolysin produced by a Kanagawa phenomenon negative clinical isolate of Vibrio parahaemolyticus 06 : K46." FEMS Microbiol. Lett.,. 48. 241-245 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, T., Ni, Y., Yoh, Y. and Miwatani, T.: "Production of monoclonal antibodies against thermostable direct hemolysin of Vibrio parahaemlyticus and application of the antibodies for enzyme-linked immunosorbent assay." Med. Microbol. Immunol.,. 78. 245-253 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, T., Ni, Y and Miwatani, T.: "Production of monoclonal antibody against a hemolysin (Vp-TRH) produced by Vibrio parahaemolyticus." FEMS Microbiol. Lett.,. 68. 167-170 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, T., Ni, Y., Hori, S., Takakura, H., Tsunasawa, S., Sakiyma, F. and T. Miwatani.: "A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus." FEMS Microbiol. Lett.,. 61. 95-100 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kato, I., Noda, M.: "ADP-ribosylation of cell membrane proteins by staphylococal alpha -toxin and leukocidin in rabbit erythrocytes and polymorpho nuclear leukocytes." FEBS Lett.255. 59-62 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nakazawa, T., Kimoto, M. and Abe, M.: "Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepatia." Gene. 94. 83-88 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakurai, J., Nagahama, M. and Takahashi, T.: "Contraction induced by Clostridium perfringens epsilon toxin in the isolated rat ileum." FEMS Microbiol. Lett.58. 269-272 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakurai, J., Fujii, Y., Torii, K. and Kobayashi, K.: "Dissociation of various biological activities of Clostridium perfringens alpha toxin by chemical modification." Toxion. 27. 317-323 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakurai, J., Fujii, Y. and Shirotani, M.: "Contraction induced by Clostridium perfringens alpha toxin." Toxicon. 28. 411-418 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi, H., Kubomura, S., Hirano, H., Mizue, K., Ogawa, M. and Mizuguchi, Y.: "Cloning and characterization of a gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus." FEMS Microbiol. Lett.,. 67. 339-346 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Wang, X., Noda, M. and Kato, I.: "Stimulatory effect of staphylococcalleukocidin on phosphoinositide metabolism in rabiit polymorphonuclearleukocytes." Infect. Immun.,. 58. 2745-2749 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamanaka, H., Shimatani, S., Tanaka, M., Katsu, T., Ono, B. and Shinoda, S.: "Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin." FEMS Microbiol. Lett.61. 251-256 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamanaka, H., Sugiyama, K., Furuta, H., Miyoshi, S. and Shinoda, S.: "Cytolytic action of Vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity." J. Med. Microbiol.32. 39-43 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Xia Wang: "Stimulatory effect of staphylococcal leukocidin on phosphoinotiside metabolism in rabbit polymorphonuclear leukocyte" Infection and Immunity. 58. 2745-2749 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hatsumi Taniguchi: "Cloning and characterization of a gene encoding a new thermostable hemolysin from <Vibrio>___ー <parahaemolyticus>___ー" FEMS Microbiology Letters. 67. 339-346 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroyasu Yamanaka: "Cytolytic action of Vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity" Journal of Medical Microbiology. 32. 39-43 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takashi Katsu: "Interaction of wasp venom mastparan with biomembranes" Biochimica et Biophysica Acta. 1027. 185-190 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Jun Sakurai: "Contraction induced by <Clostridium>___ー <perfringens>___ー alpha toxin" Toxicon. 28. 411-418 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takeshi Honda: "Production of monoclonal antibody against a hemolysin (VpーTRH) produced by <Vibrio>___ー <parahaemolyticus>___ー" FEMS Microbiology Letters. 68. 167-170 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Yamanaka,S.Shimatani,M.Tanaka,T.Katsu,B.Ono and S.Shinoda: "Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin" FEMS Microbiology Letters. 61. 251-256 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Miyoshi and S.Shinoda: "Inhibitory effect of α_2-macroglobulin on Vibrio vulnificus protease" Journal of Biochemistry. 106. 299-303 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Yamanaka,K.Sugiyama,H.Furuta,S.Miyoshi,and S.Shinoda: "Cytolytic action of Vibrio vulnificus hemolysin on mast cells from rat peritoneal cavity" Journal of Medical Microbiology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Honda,Y.Ni,S.Hori,H.Takakura,S.Tsunasawa,F.Sakiyama,and T.Miwatani: "A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus" FEMS Microbiology Letters. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Honda,Y.Ni,M.Yoh and T.Miwatani: "Production of monoclonal antibodies against thermostable direct hemolysin of Vibrio parahaemolyticus" Medical Microbiology and Immunology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Honda,Y.Ni,M.Yoh,and T.Miwatani: "Evidence of immunological cross-reactivity between hemolysis of Vibrio parahaemolyticus demonstrated by monoclonal antibodies" Journal of Infections Diseases. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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