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Application of New Genetic Markers on Proteins, Enzymes and DNA to Forensic Medicine.

Research Project

Project/Area Number 01304036
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

HARADA Shoji  Univ. of Tsukuba, Inst. of Com. Med., Associate Professor, 社会医学系, 助教授 (60086618)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Hisabumi  Univ. of Akita, Dept. of Legal Med., Professor, 医学部・法医学, 教授 (80108935)
UMEZU Kazuo  Univ. of Yamagata, Dept. of Legal Med., Associate Professor, 医学部・法医学, 助教授 (10091828)
SUZUKI Koichi  Osaka Medical Univ., Dept. of Legal Med., Lecturer, 法医学, 講師 (60171211)
HUKUSHIMA Hirobumi  Univ. of Shinsyu, Dept. of Legal Med., Professor, 医学部・法医学, 教授 (70135218)
YOKOI Takeshi  Univ. of Hokkaido, Dep. of Pharmac., Associate Professor, 薬学部・分析化学, 助教授 (70135226)
向山 明孝  科学警察研究所, 法医科, 室長
岩佐 峰雄  名古屋市立大学, 医学部・法医, 講師 (00021452)
寺沢 浩一  北海道大学, 医学部法医学, 助教授 (40142715)
石本 剛一  三重大学, 医学部法医学, 助教授 (30024673)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1991: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1990: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1989: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsGenetic polymorphism / Probe / Blood stain / Paternity testing / PCR / DNA fingerprinting / Serum protein / Individual identification / DNA多型 / pHYN22 / MCT118 / VNTR / GStπ gene / ORM型 / Factor1 / 唾液アミラ-ゼ / フィンガ-プリント法 / PCR法 / 蛋白多型 / 人獣鑑定 / 性別鑑定 / RFLP_S / DNA / 赤血球酵素 / 血清蛋白
Research Abstract

l. Application of Genetic Markers on DNA
Genetic palymorphism detected by using different probes were applied to paternity testing of the family in dispute to obtain the data base in Japan. The probes pYNZ22, pYN22, MR24/1, 3'-Globin, Mucin and Ha-ras were used to detect the single locus DNA polymorphism. It was found by the statistical analysis that these probes supply very useful information for paternity testing. Moreover, VNTR of 5'-flanking region of GST pi gene showed a high heterozigosity (83.3%) in Japanese using PCR technology.
An efficient method for isolating DNA from the forensic samples such as blood stains, bones and tissues were established to make individual identification. The isolated DNA samples were subjected to PCR for identifying VNTR on MCT 118 and ApoB and it was confirmed that these markers are very effective for forensic analysis. Also, pYNH24 probe was informative in the bone and tissue analysis. DNA fingerprinting detected by the single and multi loci probes was applied to testing in paternity cases in Japan.
2. Genetic Markers of Proteins and Enzymes.
We focused the serum proteins which have not yet studied in Japanese, although high polymorphisms have been reported in Caucasoid. The data concerning ZAG, IF, ORM, AHSG, Factor B have been obtainedin our country as well as in other Asian population, in addition to the racial differences of gene frequencies of these serum proteins. Moreover, Factor I, DR and Factor H were found to be useful markers for paternity testing.
Genetic analysis of saliva amylase variants has been done including the study of the difference among human and non-human primates. LDH isozyme pattern of the injured body was investigated from aspect of forensic analysis.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (119 results)

All Other

All Publications (119 results)

  • [Publications] Shoji Harada: "Genetic polymorphism of aldehyde dehydrogenase in Orientals as genetic marker of a high risk group of alcoholism." Alcoholism and Family. 67-71 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abei M,Harada S,et al.: "Immunohistochemical study of human liver Glutatione S-Tranferase isozymes with special reference to GST 1." Biochem.Biophis.Acta.995. 279-289 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 三木 敏行,中島 淑貴,中村 貴子,林田 良子,江良 章,十字 猛夫,安田 裕子,原田 勝二,他: "死亡した夫婦の実子の検査による父子及び母子鑑定例ー半同胞の鑑定例." 犯罪誌. 56. 263-274 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 原田 勝二: "アルコ-ル代謝酵素のDNAによる解析" アルコ-ル代謝と肝. 9. 23-26 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harihara S,Harada S,Ostubo A,Misawa S,Nakamura T,: "Lack of variant in S-adenosyl 1-homocysteine hydrolase (SAHH) among Japanese." J.Anthrop.Soc,Nippon. 98. 353-357 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S: "Genetic Polymorphism of Alcohol Metabolyzing Enzymes and its Implication to Human Ecology" J.Anthrop.Soc.Nippon. 99. 123-139 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 原田 勝二: "アルコ-ル代謝酵素の遺伝型分類によるエタノ-ルおよびアセトアルデヒドの代謝能。" アルコ-ル代謝と肝. 10. 1-5 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 原田 勝二: "アルコ-ル代謝酵素の分子遺伝学的解析。肝とアルコ-ル・肝再生・肝細胞障害" 中外医学社. 20-27 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S et al.: "Detection of GST1 gene deletion by PCR and its possible correlation with stomach cancer in Japanese." Human Genetics. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 笠井 賢太郎,向山 明孝: "PCR法の法医学への応用" 蛋白質核酸酵素. 35. 3150-3156 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 坂井 活子,吉田 日南子,笠井 賢太郎,向山 明孝: "血痕からのDNA精製法とシングルロ-カスVNTRを用いたDNA型検出法について" 科警研報告. 44. 36-49 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 吉田 日南子,坂井 活子,笠井 賢太郎,向山 明孝: "ヒト精液斑からのDNA精製法とDNA型検出法" 科警研報告. 44. 23-35 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I.Sakai,K.Yoshida,K.Kasai,H.Mudoyama: "DNA analysis using single locus VNTR pobes and its application to the individualization of human bloodstains." Proc.Internat.Symp.Adev.in Legal Medicine,Kanazawa,. 462 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Sagisaka K: "Haptoglobin typing of human bloodstans using a specific DNA probe." Fore.Sci.Inter.45. 39-46 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Suzuki,S.Ito,S.Mutoh,H.Matsumoto,G.Cey-Bery.: "A BF subtype "Fb1" is a marker gene of some Mongoloid populations." Jpn J Hum Geneticis. 35. 169-177 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Suzuki,H.Matsumot,S.Ito,Y.Mizoi: "Hereditary subtypic patterns detected in the Ba fragment of complement factor B:Occurrence of four common alleles in Japanese." Electrophoresis. 11. 835-839 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Suzuki,H.Matsrmoto,S.Ito,Y.Mizoi: "THREE HEREDITARY SUBTYPIC PATTERNS IN THE Ba FRGAMENT OF COMPLEMENT FACTOR B." PROCEEDINGS of the First International Symposium ADVANCES IN LEGAL MEDICINE.(edited by Taizo Nagano). 435-437 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Fukushima,H.Hasekura,M.Ota,: "Using the immunostaining method and the polymerase chain reaction amplification for identifying mixed-up formalin-fixed,paraffin-embedded tissues." Proc internatl Symp Advances in Legal Medicine,Kanazawa (Edited by T,Nagano). 263-265 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Ota,N.Sugimura,K.Tsuji,T.Seki,H.Mizuki,H.Inoko,K.Momura,H.Fukushima,: "Modified PCR-RFLP method for HLA-DPB1 and DQA1 genotyping." Tissue Antigens. 38. 60-71 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小林 了,飯塚 直人,日比野 隆彦,野々村 真一,宮田 瞳,松沢 茂隆: "Nonーisotopic Southern hybridization法による血痕の性別鑑別ーとくに実地応用例についてー" 日法医誌. 43. 58-63 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R.Kobayashi,S.Matsuzawa: "Sex determination of bloodstains by the use of a ribonucleic acid (RNA) probe." J Foresic Sci,. 34. 1078-1081 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I.Yuasa,M.Iha,K.Umetsu,H.Hirata,K.Suenaga,J.Ikebuchi: "Orosomucoid (ORM) typing by isoelectric focusing:An analysis of ORM haplotypes." Hum Hered. 40. 267-271 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Ding,W.Y.Choi,K.Umetsu,J,Jia,N.Nakazyashiki,T.Suzuki: "Distribution of human Zn-alpha-2-glycoprotein types in Chinese and Korean populations." Hum Hered. 40. 311-312 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.Harada,N.Ikeda,K.Umetsu,T.Suzuki,I.Yuasa,: "Orosomucoid 1 phenotyping from human urine by isoelectric focusing." J Forens Sci. 35. 1191-1195 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I.Yuasa,T,Ishida,K.Umetsu,O.Takenaka,T.Shatake,K.Terao,et al.: "Orosomucoid typing by isoelectric focusing:Genetic variation of orosomucoid in Asian macaques (genus Macaca)." Electrophoresis. 11. 840-845 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] F.M.Salzano,F.L.Black,K.Umetsu,T.Suzuki,I.Yuasa,: "Isoelectric focusing studies in Brazilian Indians-Uncovering variation of ORM,AHSG and IF-." Jpn J Human Genet. 35. 283-290 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Ding,S.Nakamura,K.Umetsu,W.Choi,I.Yuasa,T.Suzuki: "Polymorphism of complement component I in Mongoloid populations:A new genetic variant IF A2." Hum Hered. 41. 206-208 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Umetsu,I.Yuasa,M.Ding,T.Suzuki,Y.Hayashida,: "Distribution of alpha-2-HS glycoprotein types in four East Asian populations." J Anthrop Soc Nippon. 99. 371-375 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Umetsu,K.Yamashita,T.Suzuki: "Purification and carbohydrate-binding specificites of a blood type B binding lectin from hemolymoph of Crab (Charibdis Japonica)." J Biochem. 109. 718-721 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中村 茂基,溝口 彰子: "日本人におけるFactor Iの遺伝的多型:新たに見い出された対立遺伝子の記載" Jpn J Human Genet. 35. 94 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Odaira T,Nata M,Sagisaka K: "Hypervariable polymorphic VNTR loci for parentage testing and individual identification." Jpn.J.Hum.Genet.35. 179-199 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Kimura T,Sagisaka K: "Individual identification of human bloodstans with hypervariable region DNA probse." Tohoku J.Exp.Med.161. 9-18 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Odaira T,Nata M,Sagisaka K: "Hypervariable regions of DNA for parentage testing and individual identification." Zeitchrift.Rechtmedicine. 103. 487-497 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Odaira T,Sagisaka K: "investigation of paternity establishing without the putative father using hypervariable DNA probes." Jpn.J.Hum.Genet.35. 235-244 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Aoki Y,Nata M,Sagisaka k: "An unexcluded paternity case with hypervariable region DNA loci." Transfusion,. 30. 819-823 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T,Aoki Y,Odaira T,Sagisaka K,Nata M,: "Application of single-locus hypervariable region DNA probes to deficiency cases in paternity testing." Int.J.Leg.Med.,. 104. 117-120 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 那谷 雅之,匂坂 馨,横井 毅,平岩 幸一,青木 康博,高取 健彦: "Single locus DNA probeを用いた親子鑑定" 日本法医学雑誌. 45. 138-145 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 吉岡 尚文,目黒 ひとみ,横井 毅,森田 香,那谷 雅之,匂坂 馨: "血清ハプトグロビン欠損の遺伝学的検討" 生物物理化学. 35. 63-67 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakamura,A.Sawaguchi,H.Nishimukai,M.Zhou: "Factor I refernce typing report." Complement and inflammation. 7. 248-251 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakamura,O.Ohue,A.Sawaguchi: "Genetic polymorphism of huma factor H (β1H globulin)." Hum Hered. 40. 121-126 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakamura,K.Yamashita,K.Omoto,J.Ochi,O.Ohue,K.Akiyama,et al.: "C1R polymorphism in the Japanese population." Proceedings of the first Intermational Symposium Advances in Legal Medicine. 438-440 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakamura,K.Omoto,A.Sawaguchi: "Genetic polymorphism of factor I in the Japanese population:Allele frequencies in Iwate prefecture" Japan.Jpn J Hum Genet. 36. 67 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中村 茂基,秋山 勝則,中田 伸子,溝口 彰子: "補体系蛋白の遺伝的多型の解析ーFactor B (BF) のsubtypeについてー" 日法医誌. 45. 188 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakamura,A.Sawaguchi: "Factor I (IF) polymorphism in the Japanese population:Existence of a new variant and quantitative analysis of different allotypes." Gene Geography. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakamura,K,Omoto,A,Sawaguchi: "Allele frequencies of human complement factor I (IF) in a sample from Iwate,northem Japan,with the descritpion of geographical cline." Hum Hered. 41. 403-408 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 原田 勝二編・著: "ヒトDNA polymorphism,検出技術と応用" 東洋書店, 299 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S: "Genetic polymorphism of aldehyde dehydrogenasien Orientals as genetic marker of a high risk group of alcoholism." Alcoholism and Family. 67-71 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abei M, Harada S, et al.: "Immunohistochemical study of human liver Glutathione S-Transferase isozymes with special reference to GST1." Biochem. Biophis. Acta.995. 279-289 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harihara S, Ostubo A, Nakamura T, Harada S, Misaw S: "Lack of variant in S-adenosyl 1-homocysteine hydrolase (SAHH) among Japanese." J. Anthrop. Soc. Nippon. 98. 353-357 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S: "Genetic Polymorphism of Alcohol Metabolyzing Enzymesand its Implication to Human Ecology." J. Anthrop. Soc. Nippon. 99. 123-139 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S et al.: "Detection of GST1 gene deletion by PCR and its possible correlation with stomach cancer in Japanese." Human Genetics. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai I, Kasai K, Yoshida K, Mukoyama H: "DNA analysis using single locus VNTR probes and its application to the individualization of human bloodstains." Proc. Internat. Symp. Adev. in Legal Medicine, Kanazawa. 462. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Sagisaka K: "Haptoglobin typing of human bloodstains using a specific DNA probe." Fore. Sci. Inter.45. 39-46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki K, Mutoh S, G. Cey-Bery, Ito S, Matsumoto H: "A BF subtype "Fb1" is a marker gene of some Mongoloid populations." Jpn J Hum Genetics. 35. 169-177 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki K, Ito S, Matsumoto H, Mizoi Y: "Hereditary subtypic patterns detected in the Ba fragment of complement factor B : Occurrence of four commonalleles in Japanese." Electrophoresis. 11. 835-839 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki K, Ito S, Matsumoto H, Mizoi Y: "Three hereditary subtypic patterns in the Ba fragment of complement factor B." Proceedings of the First International Symposium Advances in Legal Medicine.435-437 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fukushima H, Ota M, hasekura H: "Using the Immunostaining method and the polymerase chain reaction amplification for identifying mixed-up formalin-fixed, paraffin-embedded tissues." Proc. Internatl. Symp. Advances in Legal Medicine, Kanazawa. 263-265 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ota M, Seki T, Momura K, Sugimura N, Mizuki H, Fukushima H, Tsuji K, Inoko H: "Modified PCR-RFLP method for HLA-DPB1 and DQA1 genotyping." Tissue Antigens. 38. 60-71 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kobayashi R, Matsuzawa S: "Sex determination of bloodstains by the use of a ribonucleic acid (RNA) probe." J forensic Sci. 34. 1078-1081 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuasa I, Umetsu K, Suenaga K, Iha M, Hirata H, Ikebuchi J: "Orosomucoid (ORM) typing by isoelectric focusing : An analysis of ORM haplotypes." Hum Hered. 40. 267-271 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Nata M, Odaira T, Sagisaka K: "Hypervariable polymorphic VNTR loci for parentage testing and individual identification." Jpn. J. Hum. Genet.35. 179-199 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Kimura T, Sagisaka K: "Individual identification of human bloodstains with hypervariable region DNA probes." Tohoku J. Exp. Med.161. 9-18 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Nata M, Odaira T, Sagisaka K: "Hypervariable regions of DNA for parentage testing and individual identification." Zeitchrift. Rechtmedicine. 103. 487-497 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Odaira T, Sagisaka K: "Investigation of paternity establishing without the putative father using hypervariable DNA probes." Jpn. J. Hum. Genet.35. 235-244 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Nata M, Aoki Y, Sagisaka K: "An unexcluded paternity case with hypervariable region DNA loci." Transfusion. 30. 819-823 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokoi T, Odaira T, Nata M, Aoki Y, Sagisaka K: "Application of single-locus hypervariable region DNA probes to deficiency cases in paternity testing." Int. J. Leg. Med.104. 117-120 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ding M, Umetsu K, Nakayashiki N, Choi W Y, Jia J, Suzuki T: "Distribution of human Zn-alpha-2-glycoprotein types Chinese and Korean populations." Hum Hered. 40. 311-312 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada A, Umetsu K, Yuasa I, Ikeda N, Suzuki T: "Orosomucoid 1 phenotyping from humanurine by isoelectric focusing." J Forens Sci. 35. 1191-1195 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuasa I, Umetsu K, et al.: "Orosomucoid typing by isoelectric focusing : Genetic variation of orosomucoid in Asian macaques (genus Macaca)." Electrophoresis. 11. 840-845 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Salzano F M, Umetsu K, Yuasa I, Black F L, Suzuki T: "Isoelectric focusing studies in Brazilian Indians -Uncovering variation of ORM, AHSG and IF-." Jpn J Human Genet. 35. 283-290 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ding M, Umetsu K, Yuasa I, Nakamura S, Choi W, Suzuki T: "Polymorphism of complement component I in Mongoloid populations : A new genetic variant IF A2." Hum Hered. 41. 206-208 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Umetsu K, Ding M, Hayashida Y, Yuasa I, Suzuki T: "Distribution of alpha-2-HS glycoprotein types in four East Asian Populations." J Anthrop Soc Nippon. 99. 371-375 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Umetsu K, Yamashita K, Suzuki T: "Purification and carbohydrate-binding specificities of a blood type B binding lectin from hemolymoph of Crab (Charibdis Japonica)." J Biochem. 109. 718-721 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura S, Nishimukai H, Sawaguchi A, Zhou M: "Factor I reference typing report." Complement and Inflammation. 7. 248-251 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura S, Ohue O, Sawaguchi A: "Genetic polymorphism of human factor H (B1H globulin)." Hum Hered. 40. 121-126 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura S, Omoto K, et al.: "C1R polymorphism in the Japanese population." Proceedings of the First International Symposium Advances in Legal Medicine. 438-440 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura S, Omoto K, Sawaguchi A: "Genetic polymorphism of factor I in the Japanese population : Allele frequencies in Iwate prefecture." Jpn J Hum Genet. 36. 67 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura S, Sawaguchi A: "Factor I (IF) polymorphism in the Japanese population : Existence of a new variant and quantitative analysis of different allotypes." Gene Geography. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura S, Omoto K, Sawaguchi A: "Allele frequencies of human complement factor I (IF) in a sample from Iwate, northen Japan, with the description of geographical cline." Hum Hered. 41. 403-408 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S: Toyo-Shoten. Human DNA Polymorphism,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada S: "Genetic polymorphism of alcohol metabolizing enzymes and its implication to Human Ecology" J.Anthrop.Soc.Nippon. 99. 123-139 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yokoi,T.,Odaira,T.,Nata,M.,Aoki,Y.and Sagisaka,K.;: "Application of singleーlocus hypervariable region DNA probes to deficiency cases in paternity testing" Int.J.Leg.Med.,. 104. 117-120 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ota M.;Inoko H.;Fukushima H.: "Application of HLAーDQA1 genotyping by PCRーRFLP method to forensic science." Act Cirm Japon. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Umetsu,K.;Yamashita,T.: "carbohydrateーbinding specificities of a blood type B binding lectin from hemolymoph of Crab (Charibdis japonica)." J Biochem,. 109. 718-721 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Nakamura,K.Omoto,A.Sawaguchi: "Allele ferquencies of human complement factor I(IF)in a sample from Iwate,northem Japan,with the description of geographical cline." Hum Hered. 41. 403-408 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Suzuki,T.Nojima,H.Matsumoto: "Application of a variable number of tandem repeat and multiーlocus fingerprint markers to paternity testing with references of conventional blood markers." Forens.Sci.Int.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 原田 勝二 編・著: "ヒトDNA polymorphism,検出技術と応用" 東洋書店, 299 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shoji Harada:"Genetic Polymorphism of Aldehyde Dehydrogenase and its Physiological Significance to Alcohol Metabolism" <Prog.>___ー<Clin.Biol.Res.>___ー. 344:. 289-294 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shoji Harada:"Genetic Polymorphism of Aldehyde Dehydrogenase in Orientals as Genetic Marker of a High Risk Group of Alcoholism" <Alcoholism and Family>___ー. 67-71 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shoji Harada:"Genetic Polymorphism of Alcohol Metabolysing Enzymes and Genetic Implication to Human Ecology" <J.Anthrop.Soc.>___ー<Nippon,>___ー. 99(2). (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shoji Harada:etal.: "Investigation of DNA Polymorphism by Amplification Strategy" <Advances in Legal Med.>___ー. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shoji Harada:":Molecular Basis on Ethnic Difference of Alcohol Metabolism and Application to Anthropology" <Nature and>___ー <Culture.>___ー<Ed.by Univ.Museum Univ.Tokyo>___ー. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二:"アルコ-ル代謝酵素のDNAによる解析" <アルコ-ル代謝と肝>___ー. 9:. 23-26 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二:"急性エタノ-ル中毒ー基礎編ー" <中毒研究>___ー. 3. 355-361 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二:"アルコ-ル耐性の個人差" <病態生理>___ー. 9(12). 948-955 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二:"エタノ-ルおよびアルデヒド代謝の人種的遺伝的要因" <医学のあゆみ>___ー. 154(13). 817-822 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二、三澤 章吾:"Glutathione SーTransferase 1 gene deletionおよびApolipoprotein Bー100 gene 3'hypervariable regionのPCR法による解析とその応用" <筑波大学代謝特別プロジェクト報告書>___ー. 2. 132-137 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二:"アルコ-ル代謝酵素の遺伝型分類によるエタノ-ルおよびアセトアルデヒドの代謝能" <アルコ-ル代謝と肝>___ー. 10. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 三木 敏行、林田 良子、原田 勝二他:"死亡した夫婦の実子の検査による父子及び母子鑑定例ー半同胞の鑑定例ー" <Act.Crim.Japon.>___ー. 56. 263-274 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.and Sagisaka,K.: "Haptoglobin typing of human bloodstains using a specific DNA probe." Fore.Sci.Inter.45. 39-46 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.,Nata,M.,Odaira,T.,and Sagisaka,K.: "Hypervariable polymorphic VNTR loci for parentage testing and individual identificaiton" Jpn.J.Hum.Genet.35. 179-199 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.,Kimura,T.and Sagisaka,K.: "Individual identification of human bloodstains with hypervariable region DNA probes." Tohoku J.Exp.Med.161. 9-18 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.,Nata,M.,Odaira,T.and Sagisaka,K.: "Hypervariable regions of DNA for parentage testing and individual identification." Zeitchrift.Rechtmedicine,. 102. 303-330 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sagisaka,K.,Aoki,Y.,Nata,M.,and Yokoi,T.: "On the proteins carring ABH epitopes in saliva and semen." Act.Crim.Japon,. 56. 189-193 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.,Odaira,T.and Sagisaka,K: "Investigation of paternity establishing without the putative father using singleーlocus hypervariable region DNA probes." Jpn.J.Hum.Genet.35. 235-244 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.,Odaira,T.,Nata,M.,Aoki,Y.and Sagisaka,K.: "Investigasion of paternity establishing without the putative father and his wife using singleーlocus hypervariable region DNA probes." Zeitshrift Rechtmed.,.

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T.,Nata,M.,Aoki,Y.and Sagisaka,K.: "An unexcluded paternity case with hypervariable region DNA loci." Transfusion. 30. 819-823 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Nakamura,K.Omoto,A.Sawaguchi:"Allele frequencies of human complement factor I (IF)in a sample from Iwate,northern Japan,with the description of geographical cline." Hum Hered.

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Nakamura,K.Omoto,A.Sawaguchi:"Distribution of allele frequencies of ClR in a sample from Iwate prefecture,northern Japan." Gene Geography.

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Fukuma,S.Kashimura,K.Umetsu,I.Yuasa,B.Nakano and I.Nakasono: "Two new orosomucoid(ORM2) variants in Japanese." Hum Hered. 40. 302-304 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Ding,K.Umetsu,N.Nakayashiki,W.Y.Choi,J.Jia,T.Suzuki: "Distribution of human Znーalphaー2ーglycoprotein types in Chinese and Korean populations." Hum Hered. 40. 311-312 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] I.Yuasa,K.Umetsu,K.Suenaga,M.Iha,H.Hirata and J.Ikebuchi:Orosomucoid(ORM) typing by isoelectric focusing:"An analysis of ORM haplotypes." Hum Hered,. 40. 267-271 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] F.M.Salzano,K.Umetsu,I.Yuasa,F.L.Black and T.Suzuki: "Isoelectric focusing studies in Brazilian Indiansーuncovering variation of ORM,AHSG and IF." Jpn J Human Genet. 35. 283-290 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田 勝二 編: "ヒトDNA Polymorphism 検出技術と応用" (株)東洋書店, 350 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 原田勝二,中村貴子,三沢章吾,中島淑貴: "Apolipoprotein B遺伝子3'hypervariable regionのPCR法による解析とその応用" 日法医誌. 44. 109 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuo Umetsu: "Genetic polymorphisms of orosomucoid and alpha-2HS-glycoprotein in Thai, Sri lankan and paraguayan populations" Jpn.J.Hum.Genet.34. 195-202 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuo Umetsu: "Orosomucoid(ORM)typing by isoelectiric focusing:description of two new alleles in a German Population and thermostability in bloodstains" Z.Rechtsmed. 102. 171-177 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 横井毅、那谷雅之、大平徹、匂坂肇: "single locus HVR(hypervariable regions)DNA probeの親子鑑定・個人識別への応用" 日法医誌. 44,. 110 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石本 剛一,石浜信之: "ヒトおよびサルの唾液と血清アミラ-ゼ" 日法医誌. 44. 240 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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