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Studies on Glycobilogy

Research Project

Project/Area Number 01304057
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionKyoto University (1990)
The University of Tokyo (1989)

Principal Investigator

KAWASAKI Toshisuke (1990)  Kyoto U., Pharmaceutical Sci., Professor, 薬学部, 教授 (50025706)

木幡 陽 (1989)  東京大学, 医科学研究所, 教授 (30030852)

Co-Investigator(Kenkyū-buntansha) YAMASHINA Ikuo  Kyoto Sangyo U., Engineering, Professor, 工学部, 教授 (70025675)
NAGAI Yoshitaka  U. Tokyo, Medical School, Professor, 医学部, 教授 (80072974)
SUZUKI Sakaru  Aichi Med. Sch., Biochemistry, Professor, 分子医化学研究所, 教授 (50022504)
SUZUKI Akemi  Tokyo Metropolitan Inst. Med. Sci., Chief, 臨床医学総合研究所, 室長 (70134533)
INOUE Keizo  U. Tokyo, Pharmaceutical Sci., Professor, 薬学部, 教授 (30072937)
大沢 利昭  東京大学, 薬学部, 教授 (40012603)
小川 智也  理化学研究所, 主任研究員 (30087572)
遠藤 正彦  弘前大学, 医学部, 教授 (20004616)
稲垣 冬彦  東京都臨床医学総合研究所, 室長 (70011757)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1989: ¥6,600,000 (Direct Cost: ¥6,600,000)
KeywordsGlycoprotein / Glycolipid / Proteoglycan / Glycan structure / Glycosyltransferase / Lectin / Glycan synthesis / Monoclonal antibody / 糖転移酵素 / エンドグリコシダ-ゼ / モノクロ-ン抗体 / エリスロポエチン
Research Abstract

1) Studies on new technologies for glycan analysis :
Specificity studies on plant lectins demonstrated that the binding specificity of a lectin is so strict that use of various lectin columns with distinct specificity in combination allow us to isolate a glycan chain from a mixture of glycans and elucidate its partial structure. A new technology in ^1H-NMR analysis (relayed HOHAHA) was introduced very effectively for the structural analysis of acidic oligosaccharides isolated from human milk. Two new types of enzymatic activities which cleave bonds in the carbohydrate-protein linkage regions were found : one (endo-alpha-NU-acetylgalactosaminidase) acted on mucin-type glycoproteins with a broad specificity and the other (endo-beta-galactosidase) acted on proteoglycans. These enzymes were partially purified.
2) Studies on biological functions of glycans :
Glycans from a cell adhesion molecule on lymphocytes were found to have tri-and tetra-anntenary complex-type glycans modified with the attachment of N-acetyllactosamine, Lewis X and sialyl Lewis X structure. The presence of a receptor specific for chondroitin 4-sulphate on fibroblasts was established. A gene encoding a macrophage lectin. asialoglycoprotein binding protein (ASGP-BP) was cloned and its base sequence was determined. The elucidated amino acid sequence of the macrophage lectin was very similar to that of the hepatic ASGP-BP but was clearly distinct from the latter. Sugar binding sites of plant lectins were elucidated by utilizing gene recombinant technology. Monoclonal antibodies recognizing cancer associated mucin-type oligosaccharides were prepared.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Takagaki,K.,Kon,A.,Kawasaki,H.,Nakamura,T.,Tamura,S.,& Endo,M: "Isolation and characterization of Patinopecten midーgut gland endoーβーxylosidase active on peptidochondroitin sulfate" J.Biol.Chem.,. 265. 854-860 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ii,M.,Kurata,H.,Itoh,N.,Yamashinao,I & Kawasaki,T: "Molecular cloning and sequence analysis of cDNA encoding the macrophage lectin specific for galactose and Nーacetyl galactosamine" J.Biol.Chem.,. 265. 11295-11298 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeuchi,M.,Takasaki,S.,Shimada,M.,& Kobata,A: "Role of sugar chains in the in vitro biological activity of human erythropoietin produced in recombinant chinese hamster ovary cells." J.Biol.Chem.,. 265. 12127-12130 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoneda,M.,Suzuki,S.,& Kimata,K.: "Hyaluronic acid associated with the surface of cultured fibroblasts is linked to aserumーderived 85kDa protein" J.Biol.Chem.,. 265. 5247-5257 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirabayashi,Y.,Hyodo,A.,Nakao,T.,Tsuchida,K.,Suzuki,Y.,Matsumoto,M.,Kon,K.,: "Isolation and characterization of extremely minor gangliosides,GM1b,GDld,in adult bovine brains as developmentally regulated antigens" J.Biol.Chem.,. 265. 8144-8151 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamashita,K.,Koide,N.,Endo,T.,Iwaki,Y.,& Kobata,A.: "Altered glycosylation of serum transferrin of patients with hepatocellular carcinoma" J.Biol.Chem.,. 264. 2415-2423 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 遠藤 正彦,高垣 啓一,中村 敏也: "エンドーβーキシロシダ-ゼ" 芝立出版、蛋白質核酸酵素, 5 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 川嵜 敏祐: "動物レクチン" 日本臨牀, 11 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takagaki, K., Kon, A., Kawasaki, H., Nakamura, T., Tamura, S., & Endo, M.: "Isolation and characterization of Patinopecten mid-gut gland endo-beta-xylosidase active on peptidochondroitin sulfate." J. Biol. Chem.265. 854-860 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] li, M., Kurata, H., Itoh, N., Yamashina, I., & Kawasaki, T.: "Molecular cloning and sequence analysis of cDNA encoding the macrophage lection specific for galactose ans N-acetyl-alpha-galactosamine." J. Biol. Chem.265,. 11295-11298 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeuchi, M., Takasaki, S., Shimada, M., & Kobara., A.: "Role of sugar chains in the in vitro biological activity of human erythropoietin produced in recombinant chinese hamster ovary cells." J. Biol. Chem.265. 12127-12130 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoneda, M., Suzuki, S., & Kimata, K.: "Hyaluronic acid associated with the surface of cultured fibroblasts is linked to aserum-derived 85kDa protein." J. Biol. Chem.265. 5247-5257 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirabayashi, Y., Hyodo, A., Nakao, T., Tsuchida, K., Suzuki, Y., Matsumoto, M., Kon, K.: "Isolation and characterization of extremely minor gangliosides, GMld, GDld, in adult bovine brains as developmentally regulated antigens." J. Biol. Chem. 265. 8144-8151 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamashita, K., Koide, N., Endo, T., Iwaki, Y., & Kobata, A.: "Altered glycosylation of serum transferring of patients with hepatocellular carcinoma." J. Biol. Chem.264. 2415-2423 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takagaki,K,Kon,A.,Kawasaki,H,Nakamura,T.,Tamura,S,& Endo,M: "Isolation and characterization of <Patinopecten>___ー midーgut gland endoーβーxylosidase active on pepeidochondroitin sulfate" J.Biol.Chem.,. 265. 854-860 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ii,M.,Kurata,H.,Itoh,N.Yamashina,I & Kawasaki,T.: "Molecular cloning and sequence analysis of cDNA encoding the macrophage lectin specific for galactose and Nーacetyl galactosamine" J.Biol.Chem.,. 265. 11295-11298 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takeuchi,M.,Takasaki,S.,Shimada,M.,& Kobata,A: "Role of sugar chains in the in vitro biological activity of human erythropoietin produced in recombinant chinese hamster ovary cells." J.Biol.Chem.,. 265. 12127-12130 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoneda,M.,Suzuki,S.& Kimata,K.: "Hyaluronic acid assoctated with the surface of cultured fibroblarts is linked to a serumーderived 85ーkDa protein" J.Biol.Chem.,. 265. 5247-5257 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirabayashi,Y.,Hyogo,A.,Nakao,T.,Tsuchida,K.,Suzuki,Y,Matsumoto,M,Kon,K.: "Isolation and characterization of extremely minor gangliosides,GMlb,GDld,in adult bovine brains as developmentally regulated antigens" J.Biol.Chem.,. 265. 8144-8151 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamashita,K.,Koide,N.,Endo,T.,Iwaki,Y.& Kobata,A: "Altered glycosylation of serum transferrin of patients with hepato cellular carcinoma" J.Biol.Chem.,. 264. 2415-2423 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 遠藤 正彦,高垣 啓一,中村 敏也: "エンドーβーキシロシダ-ゼ" 共立出版,蛋白質核酸酵素, 5 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 川嵜 敏祐: "動物レクチン" 日本臨牀, 11 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takeuchi,M.,Inoue,N.,Strickland,T.W.,Kubota,M.,Kobata,A.: "Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells." Proc.Natl.Acad.Sci.,U.S.86. 7819-7822 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Iwamori,M.,Kawaguchi,T.,Nagai,Y.: "Differential exposure of the major gangliosides in rabbit thymocytes to vibrio cholerae neuraminidase." J.Biochem.105. 723-727 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kawano,M.,Honke,K.,Tachi,M.,Gasa,S.,Makita,A.: "An assay method for ganglioside synthase using anion-exchanger chromatography." Anal.Biochem.182. 9-15 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogawa,H.K.,Yatohgo,T.,Izumi,M.,Hayashi,M.,Seno,N.: "Diversities in amimal vitronectins.Differences in molecular weight,immunoreactivity and carbohydrate chains." Biochim.Biophys.Acta.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hirabayashi,Y.,Hirota,M.,Suzuki,Y.,Matsumoto,M.,Obata,K.,Ando,S.: "Developmentally expressed O-acetyl ganglioside GT3 in fetal rat cerebral cortex." Neuroscience Lett.106. 193-198 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inagaki,F.,Shimada,I.,Kohda,D.,Suzuki,A.,Box,A.: "Relayed HOHAHA,a useful method for extracting subspectra of individual components of sugar chains." J.Magnetic Resonance. 81. 186-190 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamashita,Y.,Imai,Y.,Osawa,T.: "α-2,3-Linked sialic acids are more abundant in CD45 antigens and leukosialins of abnormal T cells of 1pr mice than those of normal T cels." J.Biochem.106. 961-965 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakamura,K.,Hashimoto,Y.,Moriwaki,K.,Yamakawa,T.,Suzuki,A.: "Genetic regulation of GM4(NeuAc)expression in mouse erthrocytes." J.Biochem.107. 3-7 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Furuno,K.,Ishikawa,T.,Akasaki,K.,Yano,S.,Kato,K.: "Morphological localization of a major lysosomal membrane glycoprotein in the endocytic membrane system." J.Biochem.106. 708-716 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shimada,E.,Matsumura,S.: "Determination of stokes radii of hyaluronate oligosaccharides by Sephadex chromatography." J.Chromatogr.472. 145-154 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamagata,M.,Suzuki,S.,Akiyama,S.K.,Yamada,K.M.,Kimata,K.: "Regulation of cell-substrate adhesion by proteoglycans immobilized on extracellular substrates." J.Biol.Chem. 264. 8012-8018 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamamoto,S.,Hase,S.,Yamauchi,H.,Tanimoto,T.Ikenaka,T.: "Studies on the sugar chains of interferon-γ from human peripheral-blood lymphocytes." J.Biochem.105. 1034-1039 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamazaki,F.,Nukada,T.,Ito,Y.,Sato,S.,Ogawa,T.: "Stereoselective synthesis of a core glycoheptaose of bisected biantennary complex type glycan of glycoproteins." Tetrahedron Lett.30. 4417-4420 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamashita,K.,Kobata,A.,Suzuki,T.,Umetsu,K.: "Allomyrina dichotoma lectins." Methods Enzymol.179. 331-341 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Watanabe,S.,Umeda,M.,Nomura,T.,Kobayashi,T.,Inoue,K.: "Mitogenic response of near-diploid mouse cell line m5S/1M induced by epidermal growth factor." J.Cellu.Physiol.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Goso,Y.,and Hotta,K.: "Type of oligosaccharide sulphation,depending on mucus glycoprotein source,corpus or antral,in rat stomach." Biochem.J.264. 805-812 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takagi,K.,Kon,A.,Kawasaki,H.,Nakamura,T.,Endo,M.: "Preparation and application of a fluorogenic substrate for endoーβ-xylosidase." J.Biochem.Biophys.Methods. 19. 207-214 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Muramatsu,H.,Muramatsu,T.: "Analysis of glycoprotein-bound carbohydrates from pluripotent embryonal carcinoma cells by poke weed agglutinin-agarose." J.Biochem. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kitagawa,H,.Nakada,H.,Kurosaka,A.,Hiraiwa,N.,Yamashina,I.: "Three novel ologosaccharides with the sialyl-Le^a structure in human milk." Biochemistry. 28. 8891-8897 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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