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Molecular Recognition Involved in the Formation of Cell Organelles.

Research Project

Project/Area Number 01304058
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionKyushu University

Principal Investigator

KATO Keitaro  Kyushu University, Faculty of Pharmaceuticl Sciences, Professor., 薬学部, 教授 (70037571)

Co-Investigator(Kenkyū-buntansha) 橋本 隆  信州大学, 医学部, 教授 (80009935)
TSUBOI Shozo  Yamagata University, School of Medicine, Professor., 医学部, 教授 (70004554)
田代 裕  関西医大, 医学部, 教授 (40077558)
大村 恒雄  九州大学大学院, 医学研究科, 教授 (80029933)
IKEHARA Yukio  Fukuoka University, School of Medicine, Professor., 医学部, 教授 (70037612)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1990: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1989: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsCell organelle / Molecular recognition / Mitochondrion / Lysosome / Peroxisame / Endoplasmic reticulum / Endosome / Nucleus / 細胞オルガネラ / 分子認識 / 形質膜 / エンドサイト-シス / 選別シグナル / リソソ-ム / ゴルジ体
Research Abstract

1. A cytosolic factor which plays important roles in the import process of mitochondrial proteins, and the receptor of mitochondrial precursor proteins were purified with an affinity column chromatography using the presequence as a ligand. A precursor processing protease was purified to homogeneity from rat liver mitochondria and its properties were examined. 2. Full length cDNAs for three lysosomal membrane glycoproteins in rat liver were isolated and sequenced, and biosynthesis, processing and intracellular transport of these glycoproteins were studied using pulsechase experiments with primary cultured rat hepatocytes. 3. The structural requirements for the signal-anchor and stop-transfer sequences for the membrane of ER were examined using an in vitro protein translocation system. 4.70kDa peroxisomal membrane protein had the ATP-binding domain which was similar to those of a superfamily involved in membrane transport. On the treatment of isolated rat liver peroxidase with proteinase … More K, this domain (exposed to the cytosol) was cleaved off and acyl-CoA oxidase import was diminished. 5. Using two steps affinity chromatography, anti-DDDED-Sepharose and nucleoplasmin-Sepharose, a protein of 69kDa was purified from nuclear pore fraction and this protein reacted with nuclear transport signal sequences. 6. The primary structure of rat liver 5' -nucleotidase was deduced from the cDNA sequence. This enzyme was initially synthesized as a precursor, cotranslationally processed to an intermediate form containing a hydrophobic domain at the COOH terminus, and finally converted to a mature form by removal of the hydrophobic domain and by simultaneous replacement with glycosyl-phosphatidylinositol. 7. The subpopulation of endosomes was isolated by the high magnetic field separation and the density gradient centrifugation, and effect of microtubles on endocytosis of transferin was studied. 8. SARI, a yeast gene which was originally isolated as a multicopy suppressor of the sec12 temperature-sensitive mutation, encodes a 21kDa GTP-biding protein and is essential for ER-to-Golgi protein transport. Its product, Sarip, is a peripheral membrane protein mainly residing in the ER. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Y.Tanaka: "Biosynthesis,processing and intnacellular transport of lysosomal acid phoshatase in rat hepatocytes." J.Biochem.108. 278-286 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Ono: "Purification and identification of a cytosolic factor required for import of precursors of mitochondrial proteins imto mitochondria." Arch.Biochem.Biophys.280. 229-304 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Sato: "The aminoーterminalーstructures that determine topoloーgical orientation of cyt.Pー450in microsomal membrane." EMBO J. 9. 2391-2397 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Kamijo: "The 70ーkDa peroxisomal membrane protein is a member of the Mdr(Pーglycoproteim)ーrelated ATPーbinding proteim superfamily." J.Biol Chem.265. 4543-4540 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A.Yamamoto: "Characterization of the isolated membrames and the limiting membranes of antophagosomes in rat hepatoーcytes by lectin cytochemistry." J.Histochem.Cytochem.38. 573-580 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Ogata: "Membraneーanchoring domain of rat liver 5Lnucleotidase:Identification of the cooHーterminal serminal serineー523 covalently sttached vrith a glycolipid." Biochemistry.29. 563-569 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ono, H., Tuboi, S.: "Presence of the cytosolic factor stimulating the import of precursor of mitochondrial proteins in rabbit reticulocytes and rat liver cells." Arch. Biochem. Biophys.277,. 368-373 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ono, H., Tuboi, S.: "Purification and identification of a cytosolic factor required for import of precursors of mitochondria." Arch. Biochem. Biohys.280. 299-304 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ou, W., ITO, A., Okazaki, H., Omura, T.: "Purification and characterization of a processing protease from rat liver mitochondria." EMBO J.8,. 2605-2612 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato, T., Sakaguchi, M., Mihara, K., Omura, T.,: "The aminoーterminal structures that determine topological orientation of cytochrome P-450 in mitochondrial membrane." EMBO J. 9,. 2391-2397 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Himeno, M., Fujita, H., Noguchi, Y., Kono, A., Kato, K.: "Isolation and sequencing of cDNA clone encoding acid phophatase in rat liver lysosomes." Biochem. Biophys. Res. Commun.162,. 1044-1053 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanaka, Y., Harada, R., Himeno, M., Kato, K.: "Biosynthesis, processing, and intracellular transport of lysosomal acid phosphatase in rat hepatocytes." J. Biochem.108,. 278-286 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kamijo, K., Taketani, S., Yokota, S., Osumi, T., Hashimoto, T.: "The 70-kDa peroxisomal membrane protein is a member of the Mdr(P-glycoprotein)-related ATP-binding protein superfamily." J. Biol. Chem.265. 4534-4540 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, A., Masaki, R., Tashiro, Y.,: "Characterization of the isolation membranes and limiting membranes of autophagosomes in rat hepatocytes by lectin cytochemistry." J. Histochem. Cytochem.38. 573-580 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ogata, S., Hayashi, Y., Misumi, Y., Ikehara, I.: "Membrane anchoring domain of rat liver 5'-nucleotidase : Identification of the COOH-terminal serine-523 covalently attached with a glycolipid." Biochemistry. 29. 563-569 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sako, Y., Ohinishi, S.: "Subpopulation of endosomes generated at sequential stages in the endocytic pathway of asialoganglioside-containing ferrite ligands in rat liver." J. Biochem.107,. 846-853 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nakano, A., MURAMATSU, M.: "A novel GTP-binding protein, Sarlp, is involved in transport from the endoplasmic reticulum to the Golgi apparatus." J. Cell Biol.109,. 2677-2691 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Tanaka: "Biosynthesis,processing and intracellular transport of lysosomal acid phosphatase in rat hepatocytes." J.Biochem.108. 278-286 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Ono: "Purification of the putative importーreceptor for the precursor of the mitochondrial proteins." J.Biochem.107. 840-845 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] W.ーJ.Ou: "Purification and characterization of a processing protease from rat liver mitochondria" EMBO J.8. 2605-2612 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Hijikata: "Rat peroxisomal 3ーketoacylーCoA thiolase gene." J.Biol.Chem.265. 4600-4606 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Yoshimori: "Protein disulfideーisomerase in rat exocrine poncreatic cells is exported from the ER despite possessing the retention signal." J.Biol.Chem.265. 15984-15990 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Takami: "Intracellular accumulation and oligosaccharide processing of alkaline phosphatase under disassembly of the Golgi complex caused by brefeldin A." Eur.J.Biochem.194. 805-810 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Noguchi: "Isolation and sequencing of cDNA clone encoding 96 kDa siaaloglycoprotein in rat liver lysosomal membranes." Biochem.Biophys.Res.Commun.164. 1113-1120 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kumamoto: "Critical region in the extention peptide for the import of cyt.p-450(SCC)precursor into mitochondria." J.Biochem.105. 72-78 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Arakawa: "The NH_2-terminal 14-16 amino acids of mitochondrial and bacterial thiolases can direct mature ornithine carbamyltransferase into mitochondria." J.Biochem.107. 160-164 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Fujiki: "Biosynthesis of nonspecific lipid transfer protein on free polyribosomes as a larger precursor in rat liver." J.Biochem.107. 730-734 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Suzuki: "Major peroxisomal membrane polypeptides are synthesized in cultured skin fibroblasts fro patients with Zellweger syndrome." Pediatric Res.26. 150-153 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Nakano: "A novel GTP-binding protein sarlp,is involved in transport from the ER to the Golgi apparatus." J.Cell Biol.109. 2677-2691 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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