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Qualitative and quantitative analyses of mRNAs in relation to embryogenesis of the silkworm, Bombyx mori.

Research Project

Project/Area Number 01440010
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 蚕糸学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KOGA Katsumi  Kyushu Univ.,Fac.Agric.,Prof., 農学部, 教授 (40038261)

Co-Investigator(Kenkyū-buntansha) BANNO Yutaka  Kyushu Univ.,Fac.Agric.,Assistant Prof., 農学部, 助手 (50192711)
FUJII Hiroshi  Kyushu Univ.,Fac.Agric.,Associate Prof., 農学部, 助学授 (10038268)
KAWAGUCHI Yutaka  Kyushu Univ.,Fac.Agric.,Associate Prof., 農学部, 助教授 (80038306)
Project Period (FY) 1989 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 1992: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1991: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1990: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1989: ¥16,300,000 (Direct Cost: ¥16,300,000)
KeywordsmRNA / Bombyx mori / Diapause,embryonic / Heat-shock proteins / Egg mutants / Hemolymph proteins / メッセンジャ-RNA / ヒ-トショックタンパク質 / メッセンジャ-RNA, / Bombyx mori, / 胚休眠, / アルドラ-ゼアイソザイム / 母性mRNA
Research Abstract

Analyses of mRNAs by using in vitro translation system showed that the eggs of Bombyx mori exhibited very small changes in terms of quality during fertilization, onset of diapause, termination of diapause and embryogenesis. Similar results were obtained when in vivo synthesized proteins were revealed after microinjection of protein precursors. these, rather unexpected observations indicate that the post-translational modification of respective proteins may have critical roles in cellular differentiation. On the other hand, a group of hsp70 protein analogs were found to be temporarily expressed after immersion in hot HCl, the treatment usually conducted for the silkworm eggs to break diapause. The induction of these so-called "stress proteins" for the first time explains the reason why the eggs are not seriously injured by the hot acid and can undergo very normal course of development. Next, we are interested in the size of the eggs where the mRNAs reside. The size of eggs can be changed by the mutations called Giant egg, small egg etc. The mode of expression of these mutant genes was investigated and their actions were mimicked by hormone treatment. The hemolymph proteins, which may be incorporated into eggs during oogenesis and possibly affect mRNA utilization, were also investigated. The present results may contribute to a further development of modern systems for biological study.

Report

(5 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] B.Sakaguchi: "Expression of chorion multigene families of Bombyx mandarina in comparison with those of Bombyx mori." Adv.Invertebr.Reprod.5. 517-522 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] L.R.Deng: "Isolation and properties of two allelic chymotrypsin inhibitors from the hemolymph of the silkworm,Bombyx mori." Insect Biochem.20. 531-536 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kosegawa,E.: "Induction of translatable mRNA by hot-HCl treatment in post-diapause eggs of Bombyx mori." J.Seric.Sci.Jpn.60. 508-510 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kosegawa,E.: "Expression of mRNA coding for a heat-shock protein homolog in hot-HCl treated eggs of Bombyx mori." J.Seric.Sci.Jpn.60. 511-513 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kosegawa,E.: "Two-dimensional gel analyses of translation products of RNA from early post-diapause eggs of Bombyx mori." J.Seric.Sci.Jpn.61. 180-183 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito,A.: "Pattern Comparison of in vivo protein synthesis between normal and ¨kidney¨eggs of Bombyx mori(Lepidoptera:Bombycidae)." Appl.Ent.Zool.27. 167-170 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kai,T.: "Gene structure and multiple mRNA species of Drosophila melanogaster aldolase generating three isozymes with different enzymatic properties." J.Biochem.112. 677-688 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Banno,Y.: "Cytogenetical analysis of chromosomal aberration due to translocation between the 23rd and 25th linkage groups in the silkworm,Bombyx mori." Hereditas. 118.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] B.Sakaguchi: "Expression of chorion multigene families of bombyx mandarina in comparison with those of Bombyx mori." Adv.Invertebr.Reprod. 5. 517-522 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] L.R.Deng: "Isolation and properties of two allelic chymotrypsin inhibitors from the hemolymph of the silkworm, Bombyx mori." Insect Biochem.20. 531-536 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kosegawa,E: "Induction of translatable mRNAs by hot-HCl treatment in post-diapause eggs of Bombyx mori." J.Seric.Sci.Jpn. 60. 508-501 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kosegawa,E: "Expression of mRNA coding for a heat-shock protein homology in hot-HCl treated eggs of Bombyx mori." J.Seric.Sci.Jpn. 60. 511-513 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] E.Kosegawa: "Two-dimensional gel analyses of translation products RNA from early post-diapause eggs of Bombyx mori." J.Seric.Sci.Jpn. 61. 180-183 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A.Saito: "Pattern Comparison of in vivo protein synthesis between normal and "kidney" eggs of Bombyx mori (Lepidopteral: Bombycidae)." Appl.Ent.Zool. 27. 167-170 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Kai: "Gene structure and multiple mRNA species of Drosophila melanogaster aldolase generating three isozymes with different enzymatic properties." J.Biochem. 112. 677-688 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Banno: "Cytogenetical analysis of chromosomal aberration due to translocation between the 23rd and 25th linkage groups in the silkworm, Bombyx mori." Hereditas. 118.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kosegawa,E.,Sugimoto,Y.and Koga,K.: "Two-dimensional gel analyses of translation products of RNA from early post-diapause eggs of Bombyx mori." J.Seric.Sci.Jpn.61. 180-183 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Saito,A.,Kosegawa,E.,Nho,S.K.,Koga,K.,Sakagucghi,B.and Sugimoto,Y.: "Pattern Comparison of in vivo prorein synthesis between normal ¨kikney¨eggs of Bomvyx mori (Lepidoptera:Bombycidae)." Appl.Ent.Zool.27. 167-170 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sugimoto,Y.,Nisthimura,F.,Krsakabe,T.,Nagaoka,S.,Hori,K.and Koga,K.: "Internchange in the type of aldolases during embryogenesis in Bombyx mori." J.Seric.Sci.Jpn.61. 165-171 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kai,T.,Sugimoto,Y.,Kusakabe,T.,Zhang,R.,Koga,K.and Hori,K.: "Gene structure and multiple mRNA species of Drosophila melanogaster aldolase generating three isozymes with differens enzymatic proferties." J,Biochem.112. 677-688 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 河口 豊,伴野 豊,古賀 克己,藤井 博: "カイコ卵形遺伝子の形質発現と20-ヒドロキシエクダイソン" 日蚕雑. 61. 145-149 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 河口 豊,赤木 俊介,古賀 克己,藤井 博,桑野 栄一: "KK-42投与による3眠蚕の誘発と卵形成" 九大農学芸誌. 47. 1-6 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中村 運,訳・編,共同執筆: "Stenesh分子生物学辞典" 化学同人,京都, 798 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kosegawa,E.,Sugimoto,Y.and Koga,K.: "Induction of translatable mRNAs by hotーHCl treatment in postーdiapause eggs of Bombyx mori." J.Seric.Sci.Jpn.60. 508-510 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kosegawa,E.,Sugimoto,Y.and Koga,K.: "Expression of mRNA coding for a heatーshock protein homolog in hotーHCl treated eggs of Bombyx mori." J.Seric.Sci.Jpn.60. 511-513 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kosegawa,E.,Sugimoto,Y.and Koga,K.: "Twoーdimensional gel analyses of translation products of mRNA from early postーdiapause eggs of Bombyx mori." J.Seric.Sci.Jpn.

    • Related Report
      1991 Annual Research Report
  • [Publications] Saito,A.,Kosegawa,E.,Nho,S.K.,Koga,K.,Sakaguchi,B.and Sugimoto,Y.: "Comparison in pattern of in vivo protein synthesis between normal and kidney eggs of Bombyx mori(Lepidoptera:Bombycidae)." Appl.Ent.Zool.

    • Related Report
      1991 Annual Research Report
  • [Publications] 河口 豊,伴野 豊,古賀 克己: "カイコの大卵突然変異遺伝子による形質発現3.卵巣の相互交換移植" 応動昆. 35. 109-113 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kosegawa,E.,Sugimoto,Y.and Koga,K.: "Stressーinduced protein synthesis in postーdiapause eggs of Bombyx mori." Insect Biochem.

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Aratake,L.R.Deng,H.Fujii,Y.Kawaguchi and K.Koga: "Incorporation of a haemolymph αーchymotrypsin inhibitor into eggs of Bombyx mori(Lepidoptera:Bombycidae)." Appl.Ent.Zool.25. 148-150 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Sugimoto,A.Saito,E.Kosegawa,K.Koga and H.Fujii: "Comparison of egg and embryo proteins and a trial to detect proteolytic activities in eggs of Bombyx mori." Comp.Biochem.Physiol.96B. 253-256 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] L.R.Deng,H.Fujii,H.Aratake,Y.Kawaguchi and K.Koga: "Isolation and properties of two allelic chymotrypsin inhibitors from the hemolymph of the silkworm,Bombyx mori." Insect Biochem.20. 531-536 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Aratake,L.R.Deng,H.Fujii,Y.Kawaguchi and K.Koga: "Chymotrypsin inhibitors in haemolymph and eggs of the silkworm,Bombyx mori:Developmental changes in inhibitory activity." Comp.Biochem.Physiol.97A. 205-209 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Saito,S.K.Nho,E.Kosegawa,Y.Sugimoto,K.Koga and B.Sakaguchi: "The pattern of protein synthesis in eggs of Bombyx mori with special reference to the mutant“kidneyーshaped egg"" Appl.Ent.Zool.

    • Related Report
      1990 Annual Research Report
  • [Publications] E.Kosegawa,Y.Sugimoto and K.Koga: "Synthesis of stressーshock proteins after the hotーacid treatment of Bombyx mori eggs." Insect Biochem.

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Sugimoto,A.Saito,T.Kusakabe,K.Hori and Katsumi Koga: "Flow of egg white ovalbumin into the yolk sac during embryogenesis." Biochim.Biophys.Acta. 992. 400-403 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Aratake,L.H.Deng,H.Fujii and Y.Kawaguchi and K.Koga: "Incorporation of a haemolymph-chymotrypsin inhibitor into eggs of Bombyx mori(Lepidoptera:Bombycidae)." Appl.Ent.Zool.

    • Related Report
      1989 Annual Research Report
  • [Publications] B.Sakaguchi,K.Sugahara,Y.Nakajima,K.Koga,O.Ninaki,Y.Sugimoto,M.R.Goldsmith,G.D.Mazur and F.C.Kafatos: "Expression of chorion multigene families of Bombyx mandarina in comparison with those of Bombyx mori" Adv.Invertebr.Reprod.

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Sugimoto,A.Saito,E.Kosegawa,K.Koga and H.Fujii: "Comparison of egg and embryo proteins and a trial to detect proteolytic activities in eggs of Bombyx mori." Comp.Biochem.Physiol.

    • Related Report
      1989 Annual Research Report
  • [Publications] L.R.Deng,H.Fujii,H.Aratake,Y.Kawaguchi and K.Koga: "Isolation and properties of two allelic chymotrypsin inhibitors from the hemolymph of the silkworm,Bombyx mori." Insect Biochem.

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Saito,E.Kosegawa,Y.Sugimoto and K.Koga: "Changes in pattern of protein synthesis during early embryogenesis in normal and“Kidney"eggs of Bombyx mori." Insect Biochem.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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