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Structure and Function of Calmodulin-dependent Protein Kinase II and Its Physiological Significance

Research Project

Project/Area Number 01440023
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionAsahikawa Medical College

Principal Investigator

FUJISAWA Hitoshi  Asahikawa Medical College, Department of Biochemistry, Professor, 医学部, 教授 (10027039)

Co-Investigator(Kenkyū-buntansha) 木谷 隆子  旭川医科大学, 医学部, 教務職員 (70101417)
TAKEUCHI Masayuki  Asahikawa Medical College, Department of Biochemistry, Instructor, 医学部, 助手 (40226999)
ISHIDA Atsuhiko  Asahikawa Medical College, Department of Biochemistry, Instructor, 医学部, 助手 (90212886)
KAMESHITA Isamu  Asahikawa Medical College, Department of Biochemisrty, Assistant Professor, 医学部, 助教授 (60127941)
KATOH Tsuyoshi  Asahikawa Medical College, Department of Biochemistry, Instructor (60194833)
TOBIMATSU Takamasa  Asahikawa Medical College, Department of Biochemistry, Instructor (30188768)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥28,100,000 (Direct Cost: ¥28,100,000)
Fiscal Year 1991: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1990: ¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 1989: ¥13,700,000 (Direct Cost: ¥13,700,000)
KeywordsCalmodulin-dependent Protein Kinase II / Calmcium Ion / Brain / Calodulin / Tyrosine Hydroxylase / Autophosphorylation / Calmodulin-dependent protein kinase IV / Nervous System / 中枢神経機能 / セカンドメッセンジャ- / ATPγS / アイソザイム / シエプス後膜主要蛋白質(PSD) / 分子生物学
Research Abstract

Four different polypeptides (isoforms) of calmodulin-dependent protein kinase II (CaM-kinase II), alpha, beta, gamma, and delta, which derived from four different genes, were revealed by cDNA sequence analysis of the rat brain extract. RNA blot bybridization analysis of the extracts from various tissues with probes specific for the respective mRNAs showed that gamma and delta mRNAs were expressed in various tissues, while alpha and beta mRNAs were primarily, if not exclusively, expressed in brain.
Autophosphorylation of CaM-kinase II is thought to be an important selfregulatory mechanism for controlling its protein kinase activity. The enzyme that had not undergone autophosphorylation exhibited only 15% of the maximum activity even in the presence of Ca^<2+>/calmodulin, but exposure of the enzyme to Ca^<2+>/calmodulin caused rapid autophosphorylation of the enzyme on Thr^<286>, thereby converting the enzyme to the most active form. The active form had the protein kinase activity even in … More the absence of Ca^<2+>/calmodulin, although the affinity for the protein substrates were much lower in the absence of Ca^<2+> than in its presence.
Tyrosine hydroxylase catalyzing the rate-limiting step in the biosynthesis of catecholamines is known to be phosphorylated by three second messenger-responsive multifunctional protein kinases, cAMP-dependent protein kinase (A-kinase), protein kinase C (C-kinase), and CaM-kinase II. However, the enzyme was activated via phosphorylation by A-kinase, not acivated by C-kinase, and activated only in the presence of activator protein by CaM-kinase II. The possible regulatory mechanism of the enzyme activity by these three multifunctional protein kinases were suggested.
Another calmodulin-dependent protein kinase occurring almost exclusively in brain, calmodulin-dependent protein kinase IV (CaM-kinase IV), was found to show a broad substrate specificity, suggesting its physiological significance in the regulation of the brain function by Ca^<2+>.
Interestingly, CaM-kinase IV was activated by autophosphorylation but inactivated via phosphorylation by A-kinase. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (89 results)

All Other

All Publications (89 results)

  • [Publications] 木谷 隆子: "Purification and Characterization of Antizyme lnhibitor of Ornithine Decarboxylase from Rat Liver" Biochim.Biophys.Acta. 991. 44-49 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中田 裕康: "5'ーNーEthylcarboxamide [^3H] Adenosine Binding Sites of Mouse Mastocytoma P815 Cell Membranes:Characterization and Solubilization" J.Biochem.105. 888-893 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 今井 嘉紀: "Modulation of Tryptophan Hydroxylase Activity by Phospholipids:Stimulation followed by lnactivation" J.Neurochem.53. 1293-1299 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 剛志: "カルモジュリン依存性プロティンキナ-ゼIIのタ-ゲットプロティン" 実験医学. 7. 1085-1090 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 奥野 幸子: "Regulation of Human Tyrosine Hydroxylase Activity:Effects of Cyclic AMPーdependent Protein Kinase,Calmodulinーdependent Protein Kinase II,and Polyanion" FEBS Lett.253. 52-54 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 飛松 孝正: "Tissue Specific Expression of Four Types of Rat Calmodulinーdependent Protein Kinase II mRNAs" J.Biol.Chem.264. 17907-17912 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 亀下 勇: "A Sensitive Method for Detection of Calmodulinーdependent Protein Kinase II Activity in Sodium Dodecyl SulfateーPolyacrylamide Gel" Anal.Biochem.183. 139-143 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大森 行雄: "Intracellular Localization of Tyrosine Hydroxylase Immunoreactivity in the Rat Adrenal Chromaffin and Pheochromocytoma Cells" Acta Anatomica. 136. 1-8 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大森 行雄: "TyrosineーHydroxylaseーImmunoreactive Nerve Fibers in the Separated Capsule of the Rat Adrenal Gland" Acta Anatomica. 136. 49-54 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤澤 仁: "Calmodulinーdependent Protein Kinase II" BioEssays. 12. 27-29 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 奥野 幸子: "カテコ-ルアミン生合成の調節" 蛋白質・核酸・酵素. 35. 630-637 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 寺田 和豊: "Maize Leaf Phosphoenolpyruvate Carboxylase:Phosphorylation of Serー15 with a Mammalian Cyclic AMPーdependent Protein Kinase Diminishes Sensitivity to lnhibition by Malate" FEBS Lett.259. 241-242 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 奥野 幸子: "Stabilization,Purification,and Crystallization of Catalytic Subunit of cAMPーdependent Protein Kinase from Bovine Heart" Biochim.Biophys.Acta. 1038. 204-208 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤澤 仁: "カルモデュリンによる蛋白質燐酸化反応" 病態生理. 9. 15-21 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 広和: "Purification and Characterization of Calmodulinーdependent Protein Kinase II from Rat Spleen:A New Type of Calmodulinーdependent Protein Kinase II" J・Biochem.107. 802-809 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 蒔田 芳男: "Involvement of Activator Protein in the Activation of Tryptophan Hydroxylase by cAMPーdependent Protein Kinase" FEBS Lett.268. 185-188 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 鯉淵 典之: "Localization by Immunohistochemistry of Renal Ornithine Decarboxylase in the Mouse with and without Testosterone Treatment" Endocrinol.Japan.37. 555-561 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 剛志: "Calmodulinーdependent Protein Kinase II.Kinetic Studies on the Interaction with Substrates and Calmodulin" Biochim.Biophys.Acta. 1091. 205-212 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 剛志: "Autoactivation of Calmodulinーdependent Protein Kinase II by Autophosphorylation" J.Biol.Chem.266. 3039-3044 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 奥野 幸子: "Conversion of Tyrosine Hydroxylase to Stable and Inactive Form by the End Products" J.Neurochem.57. 53-60 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 池田 篤: "Studies on the Generation of Ca^<2+>/Calmodulinーindependent Activity of Calmodulinーdependent Protein Kinase II by Autophosphorylation ー Autothiophosphorylation of the Enzyme ー" J・Biol.Chem.266. 11582-11588 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大森 行雄: "Immunoelectron Microscopic Study of Tyrosine Hydroxylase Immunoreactive Nerve Fibers and Ganglion Cells in the Rat Adrenal Gland" Anatomical Record. 229. 407-414 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 船越 洋: "Different Effects of Phosphorylation of Tyrosine Hydroxylase at Serine 40 by Three Multifunctional Protein Kinases on the Activity" J.Biol .Chem.266. 15614-15620 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大森 行雄: "Exocytosis in Pheochromocytoma Cells after Exposure to a High Concentration of Potassium" Acta Anatomica. 140. 280-283 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 石田 敦彦: "「カルモジュリン依存性プロティンキナ-ゼII」" 実験医学. 9. 1479-1484 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 亀下 勇: "Phosphorylation and Functional Modification of Calmodulinーdependent Protein Kinase IV by cAMPーdependent protein kinase" Biochem.Biophys.Res.Commun.180. 191-196 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 宮野 修: "Purification and Characterization of a Brainーspecific Multifunctional Calmodulinーdependent Protein Kinase from Rat Cerebellum" J.Biol.Chem.267. 1198-1203 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤澤 仁: "多機能性カルモジュリン依存性タンパク質リン酸化酵素" 生化学. 64. 14-25 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤澤 仁: "Bioinformatics" Elsevier Science Publishers B.V., 8 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 広和: "Bioinformatics" Elsevier Science Publishers B.V., 4 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤澤 仁: "International Symposium on Oxygenases and Oxygen Activation" Yamada Science Foundation, 4 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Kitani: "Purification and Characterization of Antizyme Inhibitor of Ornithine Decarboxylase form Rat Liver" Biochim. Biophys. Acta. 991. 44-49 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Nakata: "5'-N-Ethylcarboxamide [^3H] Adenosine Binding Sites of Mouse Mastocytoma P815 Cell Membranes : Characterization and Solubilization" J. Biochem.105. 888-893 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Imai: "Modulation of Tryptophan Hydroxylase Activity by Phospholipids : Stimulation followed by Inactivation" J. Neurochem.53-4. 1293-1299 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Katoh: "Target Proteins of Calmodulin-dependent Protein Kinase II" Jikken-Igaku. 7-9. 1085-1090 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Okuno: "Regulation of Human Tyrosine Hydroxylase Activity : Effects of Cyclic AMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase II, and Polyanion" FEBS Lett.253. 52-54 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Tobimatsu: "Tissue Specific Expression of Four Types of Rat Calmodulin-dependent Protein Kinase II mRNAs" J. Biol. Chem.264-30. 17907-17912 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I. Kameshita: "A Sensitive Method for Detection of Calmodulin-dependent Protein Kinase II Activity in Sodium Dodecyl Sulfate-Polyacrylamide Gel" Anal. Biochem.183. 139-143 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Oomori: "Intracellular Localization of Tyrosine Hydroxylase Immunoreactivity in the Rat Adrenal Chromaffin and Pheochromocytoma Cells" Acta Anatomica. 136-1. 1-8 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Oomori: "Tyrosine-Hydroxylase-Immunoreactive Nerve Fibers in the Separated Capsule of the Rat Adrenal Gland" Acta Anatomica. 136-1. 49-54 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Fujisawa: "Calmodulin-dependent Protein Kinase II" BioEssays. 12-1. 27-29 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Okuno: "Regulation of Catecholamine Biosynthesis" Tanpakushitsu-Kakusan-Koso. 35-4. 630-637 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Terada: "Maize Leaf Phosphoenolpyruvate Carboxylase : Phosphorylation of Ser-15 with a Mammalian Cyclic AMP-dependent Protein Kinase Diminishes Sensitivity to Inhibition by Malate" FEBS Lett.259-2. 241-242 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Okuno: "Stabilization, Purification, and Crystallizatin of Catalytic Subunit of cAMP-dependent Protein Kinase from Bovine Heart" Biochim. Biophys. Acta. 1038. 204-208 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Fujisawa: "Protein Phosphorylation in Which Calmodulin Participates" Byotai-Seiri. 9-1. 15-21 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Sato: "Purification and Characterization of Calmodulin-dependent Protein Kinase II from Rat Spleen : A New Type of Calmodulin-dependent Protein Kinase II" J. Biochem.107. 802-809 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Makita: "Involvement of Activator Protein in the Activation of Tryptophan Hydroxylase by cAMP-dependent Protein Kinase" FEBS Lett.268-1. 185-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Koibuchi: "Localization by Immunohistochemistry of Renal Ornithine Decarboxylase in the Mouse with and without Testosterone Treatment" Endocrinol. Japon.37-4. 555-561 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Katoh: "Calmodulin-dependent Protein Kinase II. Kinetic Studies on the Interaction with Substrates and Calmodulin" Biochim. Biophys. Acta. 1091. 205-212 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Katoh: "Autoactivation of Calmodulin-dependent Protein Kinase II by Autophosphorylation" J. Biol. Chem.266. 3039-3044 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Okuno: "Conversion of Tyrosine Hydroxylase to Stable and Inactive Form by the End Products" J. Neurochem.57-1. 53-60 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. Ikeda: "Studies on the Generation of Ca^<2+>/ Calmodulin-dependent Activity of Calmodulindependent Protein Kinase II by Autophosphorylation - Autothiophosphorylation of the Enzyme -" J. Biol. Chem.266. 11582-11588 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Oomori: "Immunoelectron Microscopic Study of Tyrosine Hydroxylase Immunoreactive Nerve Fibers and Ganglion Cells in the Rat Adrenal Gland" Anatomical Record. 229-3. 407-414 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Funakoshi: "Different Effects of Phosphorylation of Tyrosine Hydroxylase at Serine 40 by Three Multifunctional Protein Kinases on the Activity" J. Biol. Chem.266. 15614-15620 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Oomori: "Exocytosis in Pheochromocytoma Cells after Exposure to a High Concentration of Potassium" Acta Anatomica. 140. 280-283 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. Ishida: "Calmodulin-dependent Protein Kinase II" Jikken-Igaku. 9. 1479-1484 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I. Kameshita: "Phosphorylation and Functional Modification of Calmodulin-dependent Protein Kinase IV by cAMP-dependent Protein Kinase" Biochem. Biophys. Res. Commun.180. 191-196 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] O. Miyano: "Purification and Characterization of a Brain-specific Multifunctional Calmodulindependent Protein Kinase from Rat Cerebellum" J. Biol. Chem.267. 1198-1203 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Fujisawa: "Multifunctional Calmodulin-dependent Protein Kinases" Seikagaku. 64-1. 14-25 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 池田 篤: "Studies on the Generation of Ca^<2+>/Calmodulinーindependent Activity of Calmodulinーdependent Protein Kinase II by AutophosphorylationーAutothiophospharylation of the Enzyme" J.Biol.Chem.266. 11582-11588 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大森 行雄: "Immunoelectron Microscopic Study of Tyrosine Hydroxylase Immunoreactive Nerve Fibers and Ganglion Cells in the Rat Adrenal Gland" Anatomical Record. 229. 407-414 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 船越 洋: "Different Effects of Phosphorylation of Tyrosine Hydroxylase at Serine 40 by Three Multifunctional Protein Kinase on the Activity" J.Biol.Chem.266. 15614-15620 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大森 行雄: "Exocytosis in Pheochromocytoma Cells after Exposure to a High Concentration of Potassium" Acta Anatomica. 140. 280-283 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 石田 敦彦: "「カルモジュリン依存性プロティンキナ-ゼII」" 実験医学. 9. 1479-1484 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 亀下 勇: "Phosphorylation and Functional Modification of Calmodulinーdependent Protein Kinase IV by cAMPーdependent Protein Kinase" Biochem.Biophys.Res.Commun.180. 191-196 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 宮野 修: "Purification and Characterization of a Brainーspecific Multifunctional Calmodulinーdependent Protein Kinase from Rat Cerebellum" J.Biol.Chem.267. 1198-1203 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤澤 仁: "多機能性カルモジュリン依存性タンパク質リン酸化酵素" 生化学. 64. 14-25 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤澤 仁: "International Symposium on Oxygenases and Oxygen Activation" Yamada Science Foundation, 4 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 広和: "Purification and Characterization of Calmodulinーdependent Protein Kinase II from Rat Spleen:A New Type of Calmodulinーdependent Protein Kinase II" J.Biochem.107. 802-809 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 奥野 幸子: "Stabilization,Purification,and Crystallization of Catalytic Subunit of cAMPーdependent Protein Kinase from Bovine Heart" Biochim.Biophys.Acta.1038. 204-208 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 寺田 和豊: "Maize Leaf Phosphoenolpyruvate Carboxylase:Phosphorylation of Serー15 with a Mammalian Cyclic AMPーdependent Protein Kinase Diminishes Sensitivity to Inhibition by Malate" FEBS Lett.259. 241-242 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 蒔田 芳男: "Involvement of Activator Protein in the Activation ofーTryptophan Hydroxylase by cAMPーdependent Protein Kinase" FEBS Lett.268. 185-188 (1990)

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      1990 Annual Research Report
  • [Publications] 加藤 剛志: "Calmodulinーdependent Protein Kinase II.Kinetic Studies on the Interaction with Substrates and Calmodulin" Biochim.Biophys.Acta.1091. 205-212 (1991)

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      1990 Annual Research Report
  • [Publications] 加藤 剛志: "Autoactivation of Calmodulinーdependent Protein Kinase II by Autophosphorylation" J.Biol.Chem.266. 3039-3044 (1991)

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      1990 Annual Research Report
  • [Publications] 奥野 幸子: "Conversion of Tyrosine Hydroxylase to Stable and Inactive Form by the End Products" J.Neurochem.(1991)

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      1990 Annual Research Report
  • [Publications] 飛松孝正: "Tissue Specific Expression of Four Types of Rat CalmodulinーDependent Protein Kinase II mRNAs" J.Biol.Chem.264. 17907-17912 (1989)

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      1989 Annual Research Report
  • [Publications] 今井嘉紀: "Modulation of Tryptophan Hydroxylase Activity by Phospholipids:stimulation followed by inactivation" J.Neurochem.53. 1293-1299 (1989)

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      1989 Annual Research Report
  • [Publications] 加藤剛志: "カルモジュリン依存性プロティンキナ-ゼIIのタ-ゲットプロティン" 実験医学. 7. 1085-1090 (1989)

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  • [Publications] 奥野幸子: "Regulation of human tyrosine hydroxylase activity:Effects of cyclic AMPーdependent protein kinase,calmodulonーdependent protein kinase II,and polyanion" FEBS Lett.253. 52-54 (1989)

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  • [Publications] 木谷隆子: "Purification and Characterization of Antizyme Inhibitor of Ornithine Decarboxylase from Rat Liver" Biochim.Biophys.Acta. 991. 44-49 (1989)

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  • [Publications] 中田裕康: "5′ーNーEthercarboxamide〔^3H〕Adenosine Binding Sites of Mouse Mastocytoma P815 Cell Membranes" J.Biochem.105. 888-893 (1989)

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  • [Publications] 藤澤仁: "Calmodulinーdependent Protein Kinase II" BioEssays. 12. 27-29 (1990)

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  • [Publications] 亀下勇: "A Sensitive Method for Detection of Calmodulinーdependent Protein Kinase II Activity in Sodium Dodecyl Sulfatepolyacrylamide Gel" Anal.Biochem.183. 139-143 (1989)

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  • [Publications] 奥野幸子: "カテコ-ルアミン生合成の調節" 蛋白質、核酸、酵素. 35. 630-637 (1990)

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  • [Publications] 大森行雄: "Intracellurar Localization of Tyrosine Hydroxylase Immunoreactivity in the Rat Adrenal Chromaffin and Pheochrocytoma Cells" Acta Anatomica. 136. 1-8 (1989)

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  • [Publications] 大森行雄: "TyrosineーHydroxylaseーImmunoreactive Nerve Fibers in the Separated Capsule of the Rat Adrenal Gland" Acta Anatomica. 136. 49-54 (1989)

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  • [Publications] 藤澤仁: "カルモデュリンによる蛋白質燐酸化反応" 病態生理. 9. 15-21 (1990)

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  • [Publications] 藤澤,仁: "Bioinformatics(Eds.by O.Hatase,J.H.Wang)" Elsevier Science Publishers B.V., 8 (1989)

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      1989 Annual Research Report
  • [Publications] 佐藤広和: "Bioinformatics(EdS.by O.Hatase,J.H.Wang)" Elsevier Science Publishers B.V., 4 (1989)

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      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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