| Project/Area Number |
01440043
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Osaka City University Medical School |
|---|
Principal Investigator |
TAKEDA Tadanao Osaka City University Medical School, First Department of Internal Medicine, Professor, 医学部, 教授 (50010129)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NEGORO Nobuo Osaka City University Medical School, First Department of Internal Medicine, Ins, 医学部, 助手 (80180701)
KOHNO Masakazu Osaka City University Medical School, First Department of Internal Medicine, Ass, 医学部, 講師 (20153489)
KANAYAMA Yoshiharu Osaka City University Medical School, First Department of Internal Medicine, Ass, 医学部, 講師 (60106341)
TANABE Kazuyuki Osaka Engineering College, Department of Biology, Associate Professor (40047410)
|
|---|
| Project Period (FY) |
1989 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥22,600,000 (Direct Cost: ¥22,600,000)
Fiscal Year 1991: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1990: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1989: ¥14,600,000 (Direct Cost: ¥14,600,000)
|
|---|
| Keywords | Protein kinase C / Spontaneously hypertensive rats / Phospholipase C / Restriction fragment length polymorphism / Platelet-derived growth factor A / Aorta / 高血圧 / 自然発症高血圧ラット / 血小板由末成長因子(PDGFーA) / 高血圧自然発症ラット(SHR) / WKYラット / 遺伝子クロ-ニング / Restriction fragment length polymorphism(RFLP) / ミオシン軽鎖 / 血小板 / リン酸化 / フオスフォライペ-スC / クロ-ニング |
|---|
| Research Abstract |
(1) Protein kinase C (PKC) activity in aorta of spontaneously hypertensive rats (SHR) was elevated compared with WKY rats or enalapril-treated SHR and was closely associated with blood pressure (r=0.84, p<0.001). PKC-alpha protein (74k, 77k) and PKC-alpha mRNA were increased in SHR aorta compared with that in WKY and reversed by enalapril treatment. Thus, PKC protein synthesis is increased in SHR aorta and can be modulated by hypotensive treatment. (2) Restriction fragment length polymorphism (RFLP) of phospholipase C (PLC)-alpha, -beta, -gamma_1, -gamma_2, -delta gene in WKY, SHR and SHRSP was investigated. RFLP in PLCalpha and -beta gene was found between SHRSP and SHR, WKY. RFLP in PLC-gamma_1 gene was found among SHRSP, SHR and WKY. No RFLP was found in PLC-gamma_2 gene. RFLP was also found between SHRSP, SHR, and WKY. (3) Gene expressions of plateletderived growth factor (PDGF)-A, PDGF-B, IL-6, TGF-beta were detected in aorta of SHR. PDGF-A was most abundantly expressed in aorta of SHR when compared with those in WKY in any age examined (4, 8, 15, 54-week-old). These results suggest that PDGF-A is important in the pathogenesis of vascular hypertrophy in hypertension.
|
