| Project/Area Number |
01440056
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
OKADA Yoshio Shiga Univ.of Med.Sci., Vice-President, 医学部, 副学長 (10106825)
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| Co-Investigator(Kenkyū-buntansha) |
ASAKURA Shoji Shiga Univ.of Med.Sci.,2nd Dep.of Surg.,Assist.Prof., 医学部, 助手 (90222570)
YASUDA Yuji Shiga Univ.of Med.Sci.,2nd Dep.of Surg.,Assist.Prof., 医学部, 助手 (60191128)
FUJINO Shozo Shiga Univ.of Med.Sci.,2nd Dep.of Surg.,Assist.Prof., 医学部, 助手 (10209075)
KATO Hirofumi Shiga Univ.of Med.Sci.,2nd Dep.of Surg.,Assist.Prof., 医学部, 講師 (20111974)
MORI Atsumi Shiga Univ.of Med.Sci.,2nd Dep.of Surg.,Professor, 医学部, 教授 (80026971)
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| Project Period (FY) |
1989 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥24,700,000 (Direct Cost: ¥24,700,000)
Fiscal Year 1992: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1991: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1990: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1989: ¥11,700,000 (Direct Cost: ¥11,700,000)
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| Keywords | Oxygenator / Preserved lung / Gas exchange ability / Preservation by simple immersion / Fixed lung / ^1H-MRI / Lung transplantation / Wet / dry weight ratio / 酸素添加装置 / 異種肺移植 / 信号強度 / 肺水分量 / 乾燥重量比 / 温阻血 / 活性化酸素 / PGEI / SOD / 細胞骨格 / 保存時間 / 肺機能 / 灌流固定 / 酸素化能 / 肺表面血流量 / 肺コンプライアンス / 定常流、拍動流 / AーB barrier |
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| Research Abstract |
The following studies were carried out for the purpose of developing a natural ventilator which retains the merits of the in vivo lung,is more durable,and has no antigenicity. 1.The development of an apparatus for functional evaluation of the resected free lung: An apparatus to preserve the resected lung by perfusion with oxygenated blood with intermittent perfusion with venous blood was developed,and airway resistance,pulmonary artery pressure,and gas exchange ability were evaluated simultaneously at various time points. 2.functional evaluation of the preserved lung: The gas exchange ability of the preserved resected lung was serially monitored with the above apparatus,and optimal conditions of preservation were evaluated. The function of the rabbit lung preserved by simple immersion was well maintained for 48 hours but was significantly reduced after 72 hours. 3.Gas exchange ability of the fixed lung: The fixation method for maximum preservation of the lung function was evaluated. Am
… More
ong 10% formalin,0.625% glutaraldehyde,4% paraformaldehyde + 0.325% glutaraldehyde,the mixture of paraformaldehyde and glutaraldehyde was the best and showed a gas exchange ability comparable to the fresh lung. 4.Perfusion with heterogeneous blood and gas exchange ability: Whether the natural lung can be used as an oxygenator across the species was examined. The resected dog lung showed sufficient gas exchange ability for rabbit blood. 5.Imaging studies of the lung by ^1H-MRI: As a basic step for the development of a heterogeneous lung graft,the lung function was studied by MRI. The dynamics of the water in the lung,which determines its gas exchange ability,was evaluated according to MR images. The values of various parameters calculated in the preserved rabbit lung changed with the wet/dry weight ratio,which is an index of the water content of the lung.
These findings suggest that the resected lung retains gas exchange ability comparable to that of the fresh lung if it has been fixed under appropriate conditions,that heterogeneous lungs show a gas exchange ability,that the gas exchange ability of the lung is reduced by thickening of the air-blood barrier due to edematous changes,and that MRI is useful for noninvasive detection of these changes. Improvements in the method for fixation of the lung are desired,and,for this purpose,the development of techniques that do not leave even a trace amount of the fixative or techniques for physical fixation is needed. Less
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