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Comparative Study of Allograft Rejection in Man and Rat : Analysis of Activated Monocytes Involing in Acute Rejection.

Research Project

Project/Area Number 01440067
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionOsaka University Medical School

Principal Investigator

SONODA Takao  Professor, Osaka University Medical School, 医学部, 教授 (80028290)

Co-Investigator(Kenkyū-buntansha) KOKADO Yukito  Assistant Professor Osaka University Medical School, 医学部, 助手 (30186639)
TAKAHARA Shiroh  Assistant Professor Osaka University Medical School, 医学部, 助手 (70179547)
ISHIBASHI Michiko  Lectuler Osaka University Medical School, 医学部, 講師 (40107032)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 1990: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1989: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsAllograft rejection / Activated monocytes / Complement receptor 3 / Adhesion molecules / Hemolysis / 拒絶反応 / 皮膚移植 / complement receptor 3 / 活性化マクロファ-ジ / サイクロスポリン
Research Abstract

Purpose : To find a new immunological indicator for allograft rejection in animal model of experimental transplantation, we investigated whether a SPFC (spontaneous plaque-forming cell) as activated monocytes was involved in allograft rejection and whether adhesion molecules of complement receptor 3 (CR3) was involved in hemolysis of SPFC.
Methods and Results : (1) To evaluate SPFC response in skin allograft transplantation of rats, two different combinations of rats disparate with a H-1 histocompatibility locus was chosen. SPFC effector was identified in the infiltrating cells of rejecting transplanted grafts. Numbers of SPFC-effector at peak response of each group in the two experimental transplantation was significantly different. Since SPFC is regulated by a subsets of T-lymphocytes in man, the present findings suggested that SPFC in rats also may be regulated by T-lymphocytes and reflected a histoincompatibility. (2) The Mo-1, anti-human CR3 monoclonal antibody suppressed in vitro hemolysis of SPFC in man by adding the SPFC assay. The OX42, anti-rat CR3 monoclonal antibody also inhibited in vitro hemolysis of SPFC in rats. The CR3 molecule involves in hemolysis of SPFC in both man and rat.
Conclusions : The SPFC as cellular effector of activated monocytes may be a useful immunologic indicator for manipulating allograft rejection.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] M.Ishibashi,et al.: "Expression of adhesion promoting receptors on activated monocytes involved in hemolysis." Proc.Jpn.Soc.Immunol.,. 20. 297 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Abderlhakim,et al.: "Monocyte hemolytic effector as immunologic indicator of allograft rejection in rat skin transplantation." Transplantotio Japonica,. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 石橋 道男 ほか: "ラット同種拒絶反応におけるマクロファ-ジ・単球エフェクタ-の関与:新しい単球エフェクタ-spontaneous plaqueーForming cellについて。" 泌尿器科紀要. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ishibashi, M.: "Immunopharmacologic effects of immunosuppressive agents explored by a new effector monocyte generation assay." Transplant. Proc.21. 1854-1858 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Moutabarrik, A.: "Cellular hemolytic response by a new monocyte effector in rat alloskin graft rejection." Proc. Jpn. Soc. Immunol.19. 452-452 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ishibashi, M.: "Expression of adhesion promoting receptors on activated monocyte involved in hemolysis." Proc. Jpn. Soc. Immunol.20. 297-297 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Moutabarrik, A.: "Monocyte hemolytic effector as immunologic indicator of allograft rejection in rat skin transplantation." Transplantation. Japonica.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Ishibashi,et al.: "Expression of adhesion promoting receptors on activated monocytes involved in hemolysis." Proc.Jpn.Soc.Immunol.,. 20. 297 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Abderlhakim,et al.: "Monocyte hemolytic effector as immunologic indicator of allograft rejection in rat skin transplantation." Transplantotio Japonica,. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 石橋 道男ほか: "ラット同種拒絶反応におけるマクロファ-ジ・単球エフェクタ-の関与:新しい単球エフェクタ-spontaneous plaqueーForming Cellについて。" 泌尿器科紀要. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ishibashi M.:"Immunopharmacologic effects of immunosuppressive agents explored by a new effector monocyto generatin assay" Transpl.Proc.,. 21. 1854-1858 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Moutabarric A.:"Cellular hemolytic response by a new monocyte effector in rat alloskin graft rejection." Proc.Jpn.Soc.Immunol.,. 19. 452 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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