| Project/Area Number |
01440086
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
医学一般
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HOMMURA S. (1991) Univ, of Tsukuba, Insti. of Clin. Med. Professor, 臨床医学系, 教授 (30110505)
安羅岡 一男 (1989-1990) 筑波大学, 基礎医学系・寄生虫学, 教授 (00015976)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Y. Univ. of Tsukuba Insit. of Clin. Med. Assistant Professor, 臨床医学系, 講師 (80151405)
IRIE Y. Univ. of Tsukuba, Insti. of Basic Sci. Associate Professor, 基礎医学系, 助教授 (80110485)
ISHII K. Univ. of Hosei, Dept. of biology Professor, 教養部, 教授 (40060983)
本村 幸子 筑波大学, 臨床医学系・眼科学, 教授 (30110505)
小山 力 国立予防衛生研究所, 寄生虫部, 部長 (70072874)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1991: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1990: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1989: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | Acanthamoeba / Keratitis / DNA diagnosis / Distribution / Proliferation rate / Contact lens / Triazole antifungals / Debridement / 標準培養法 / 殺シスト / 3者併用療法 / 過酸化水素 / 臨床像 / 角膜アメ-バ症 / イトラコナゾ-ル / フルコナゾ-ル / シストの土壤内地理的分布 / シストの分類学的標徴の変化 / シストの微細構造 / ミトコンドリアDNA / 分布 / コ-ルド滅菌 |
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| Research Abstract |
Amoeba from corneal infection and soil were classified by typing of mitochondrial DNA. PCR method was useful in rapid diagnosis of Acanthamoeba keratitis. Amoebic plaque was seen on the surface of agar in culture by yeast-NN agar. Amoeba proliferation rate was calculated by computer. Amoeba was cultured from soil at 70.5% in Sapporo, 73.9% in Tokyo, 57.6% in Naha.
Cyst was transported by wind. Morphological change was minimum at 10-37ﾟC in culture. We had seven patients with Acanthamoeba keratitis. Direct examination by various stains was most important in diagnosis. At present, combined therapy with systemic triazole antifungals, topical miconazole, and surgical debridement of the lesion was the best treatment. Amoeba were cultured in 96 healthy contact lens wearers' preservatives at 10% ratio.
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