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Dynamics of Gestalt Polymer Chains in Gel and Separation Mechanism of DNA under Gel Electrophoresis

Research Project

Project/Area Number 01470107
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子物性
Research InstitutionOsaka University

Principal Investigator

KOTAKA Tadao  Osaka Univ. Fac. Science Professor, 理学部, 教授 (20027022)

Co-Investigator(Kenkyū-buntansha) SHIKATA Toshiyuki  Osaka Univ. Fac. Science Research Associate, 理学部, 助手 (10178858)
WATANABE Hiroshi  Osaka Univ. Fac. Science Research Associate, 理学部, 助手 (90167164)
ADACHI Keiichiro  Osaka Univ. Fac. Science Associate Professor, 理学部, 助教授 (00028226)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsDNA / Gel Electrophoresis / Mobility / Polyisoprene / Polybutadiene / Dielectric Relaxation / Polymer Gel / Pin-Down Frequency / パルスフィ-ルドゲル電気泳動 / 配向緩和
Research Abstract

The object of this study was to clarify dynamics of gestalt polymeric chains in gels, and to clarify the mechanism of molecular weight dependent separation in gel electrophersis of DNA. We also attempted to establish the optimum electropheric condition that enabled us to separate enormously large DNA more than 10^6 basepairs (bp). First, we examined dielectric relaxation of gest polyisoprene, PI, that exhibits the dielectric normal mode prucess, dissolved in much longer PI matrix, in polybutadiene (PB) matrix, and also in crosslinked PB matrix with varying molecular weight M. Then, we found that when gest chains were too short to entangle with the gel matrix, their relaxation time was proportinal to M^2, and this could be explained by Rouse-Zimm theory. On the other hand, when the gest chains was so long as to entangle with the gel, the relaxation time was proporinal to M^3, and this well suited with Doi-Edowards theory that is invalid in monodisperse systems.
Then, we proposed a new electric field that has a sinusoidal field with amplitude E_s and frequency f superposed on a steady biase field E_b ; Biased Sinusolidal Field. We found that in a condition of E_b < E_s, DNAs longer than 20 x 10^3 bp had minimum in mobilityat a particular M-dependent frequency, which was called pin-down frequency, f_p. We also confirmed the relation of f_p alpha M^<-1>C_<gel>^<-1>E_S^0E_b^1 from experiments with various agarose gel concentration C_<gel>. Biased reptation model explained molecular weight dependence of mobility in steady field electrophoresis qualitatively, but not quantitatively. More realistic model describing dynamics of large DNA in gel electrophoresis is required for more detailed discussion.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Hirotugu YOSHIDA,: "“Dielectric Normal Mode Process of StarーShaped Polyisoprenes"" Polymer Journal. 21. 863-872 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI: "“Swelling Equilibrium of Solution Crossーlinked Polybutadiene Networks in Polyisoprene Solutions"" Macromolecules. 22. 3106-3111 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI: "“A Dielectric Study of the Dynamics of Polyisoprene Trapped in Polybutadiene Networks"" Macromolecules. 22. 3111-3116 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi WATANABE: Polymer Journal. 22. 153-161 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI: "“Dielectric Normal Mode Process in Binary Blends of Polyisoprene.1.Excluded Volume Effect in Undiluted Binary Blends"" Macromolecules. 23. 2550-2554 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI: "“Dielectric Normal Mode Process in Binary Blends of Polyisoprene.2.Dynamic Behavior"" Macromolecules. 23. 2554-2559 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirotsugu YOSHIDA: "“Entanglement in Blends of Monodisperes Star and Linear Polystyrenes.2.Concentrated Blends"" Macromolecules. 24. 572-577 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI: "“Dielectric normalーmode process of polyisoprene in blends and in networks"" Journal of NonーCrystalline solids. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiyuki SHIKATA: "“Biased Sinusoidal Field Gel Electrophoresis for SizeーDependent DNA Separation"" Biopolymers. 31. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiyuki SHIKATA: "“Biased Sinusoidal Field Gel Electrophoresis for Large DNA Separation"" Macromolecules. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadao KOTAKA: "“Structure of multiphase polymers" “Frontiers of Macromolecular Science," IUPAC 32nd Int'l Symposium on Macromolecules August 1ー5,1988,Kyoto." Blackwell Scientific Publications,Oxford & London, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirotugu YOSHIDA, Keiichiro ADACHI, Hiroshi WATANABE, and Tadao KOTAKA: ""Dielectric Normal Mode Process of Star-Shaped Polyisoprenes"" Polymer Journal. 21. 863 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI, Tetsuo NAKAMOTO and Tadao KOTAKA: ""Swelling Equilibrium of Solution Cross-linked Polybutadiene Networks in Polyisoprene Solutions"" Macromolecules. 22. 3106 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI, Tetsuo NAKAMOTO, and Tadao KOTAKA: ""A Dielectric Study of the Dynamics of Polyisoprene Trapped in Polybutadiene Networks"" Macromolecules. 22. 3111 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi WATANABE, Hirotsugu YOSHIDA, and Tadao KOTAKA: ""Model Calculation for Viscoelastic and Dielectric Relaxation functions of Non-entangled Star Chains with Arm Length Distribution"" Polymer Journal. 22. 153 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI, Isao NISHI, Shigeru ITOH, and Tadao KOTAKA: ""Dielectric Normal Mode Process in Binary Blends of Polyisoprene. 1. Excluded Volume Effect in Undiluted Binary Blends"" Macromolecules. 23. 2550 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI, Shigeru ITOH, Isao NISHI, and Tadao KOTAKA: ""Dielectric Normal Mode Process in Binary Blends of Polyisoprene. 2. Dynamic Behavior"" Macromolecules. 23. 2555 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirotsugu YOSHIDA, Hiroshi WATANABE, and Tadao KOTAKA: ""Entanglement in Blends of Monodisperse Star and Linear Polyrstyenes. 2. Concentrated Blends"" Macromolecules. 24. 572 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Keiichiro ADACHI and Tadao KOTAKA: ""Dielectric normal-mode process of polyisoprene in blends and in networks"" Journal of Non-Crystalline solids. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiyuki SHIKATA and Tadao KOTAKA: ""Biased Sinusoidal Field Gel Electrophoresis for Size-Dependent DNA Separation"" Biopolymers. 31. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiyuki SHIKATA and Tadao KOTAKA: ""Biased Sinusoidal Field Gel Electrophoresis for Large DNA Separation"" Macromolecules. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadao KOTAKA: Blackwell Scientific Publications, Oxford & London. "Structure of multiphase polymers" in T. Saegusa, T. Higashimura, and A. Abe, eda. "Frontiers of Macromolecular Science, " 387 ; IUPAC 32nd Int'l Symposium on Macromolecules August 1-5, 1988. Kyoto., (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirotugu YOSHIDA.: "“Dielectric Normal Mode Process of StarーShaped Polyisoprenes"" Polymer Journal. 21. 863-872 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Keiichiro ADACHI: "“Swelling Equilibrium of Solution Crossーlinked Polybutadiene Networks in Polyisoprene Solutions"" Macromolecules. 22. 3106-3111 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Keiichiro ADACHI: "“A Dielectric Study of the Dynamics of Polyisoprene Trapped in Polybutadiene Networks"" Macromolecules. 22. 3111-3116 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroshi WATANABE: "“Model Calculation for Viscoelastic and Dielectric Relaxation functions of Nonーentangled Star Chains with Arm Length Distribution"" Polymer Journal. 22. 153-161 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Keiichiro ADACHI: "“Dielectric Normal Mode Process in Binary Blends of Polyisoprene.1.Excluded Volume Effect in Undiluted Binary Blends"" Macromolecules. 23. 2550-2554 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Keiichiro ADACHI: "“Dielectric Normal Mode Process in Binary Blends of Polyisoprene.2.Dynamic Behavior"" Macromolecules. 23. 2554-2559 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirotsugu YOSHIDA: "“Entanglement in Blends of Monodisperse Star and Linear Polystyrenes.2.Concentrated Blends"" Macromolecules. 24. 572-577 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Keiichiro ADACHI: "“Dielectric normalーmode process of ployisoprene in blends and in networks"" Journal of NonーCrystalline solids. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshiyuki SHIKATA: "“Biased Sinusoidal Field Gel Electrophoresis for SizeーDependent DNA Separation"" Biopolymers. 31. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshiyuki SHIKATA: "“Biased Sinusoidal Field Gel Electrophoresis for Large DNA Separation"" Macromolecules. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadao KOTAKA: "“Structure of multiphase polymers" “Frontiers of Macromolecular Science,"IUPAC 32nd Int^'l Symposium on Macromolecules August 1ー5,1988,Kyoto." Blackwell Scientific Publications,Oxford & London, 6 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Keiichiro Adachi,Tetsuo Nakamoto,and Tadao Kotaka: "A Dielectric Study of the Dynamics of Polyisoprene Trapped in Polybutadiene Networks" Macromolecules. 22. 3106-3111 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Keiichiro Adachi,Tetsuo Nakamoto,and Tadao Kotaka: "Swelling Equilibrium of Solution Cross-Linked Polybutadiene Networks in Polyisoprene Solutions" Macromolecules. 22. 3111-3116 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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