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Production of D-pantothenate with Microbial Enzymes

Research Project

Project/Area Number 01470124
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionKyoto University

Principal Investigator

YAMADA Hideaki  Kyoto University Agric. Professor, 農学部, 教授 (30027180)

Co-Investigator(Kenkyū-buntansha) NAGASAWA Toru  Kyoto Univ. Agriculture Assistant Prof., 農学部, 助手 (60115904)
SHIMIZU Sakayu  Kyoto Univ. Agriculture Assoc. Prof., 農学部, 助教授 (70093250)
IZUMI Yoshikazu  Tottori University Engineering Professor, 工学部, 教授 (40026555)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsD-Pantothenic acid / D-Pantoyl lactone / Carbonyl reductase / Stereospecific reduction / D-パントテン酸 / D-パントイルラクトン / カルボニルレダクタ-ゼ
Research Abstract

New NADPH-dependent carbonyl reductases which catalyzes stereospecific reductions of carbonyl compounds have been purified from various microorganisms and characterixed. The enzyme "polyketone reductase" from Candida parapsilosis specifically catalyzes reduction of various prochiral diketones, yielding the corresponding (R) - hydroxyketones. Aldehyde reductase from Sporobolomyces salmonicolor and carbonyl reductase from Candida macedoniensis catalyze stereospecific reduction of various aliphatic and aromatic ketones and aldehydes. Ketopantoic acid reductase from Pseudomonas maltophilia and an FMN-dependent alcohol dehydrogenase from Nocardia asteroides, catalyze reduction of ketopantoic acid to D-pantoic acid and oxidation of L-pantoyl lactone to ketopantoyl lactone, respectively. These enzymes were successfully applied to the synthesis of chiral intermediates for the syntesis of D-pantothenate.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report

Research Products

(21 results)

All Other

All Publications (21 results)

  • [Publications] Hiroyuki Hata: "Ketopantoylーlactone reductase from Candida parapsilosis;purification and characterization as a conjugated polyketone reductase" Biochim.Biophys.Acta. 990. 175-181 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroyuki Hata: "Ketopantoyl lactone reductase is a conjugated polyketone reductase" FEMS Microbiol.Lett.58. 87-90 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Kataoka: "Novel enzymatic production of Dー(ー)ーpantoyl lactone through the stereospecific reduction of ketopantoic acid" Agric.Biol.Chem.54. 177-182 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Kataoka: "Microbial production of chiral pantothenonitrile through stereospecific reduction of 2'ーketopantothenonitrile" Biotechnol.Lett.12. 357-360 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Hata: "Stereoselective reduction of diketones by a novel carbonyl reductase from Candida parapsilosis" J.Org.Chem.55. 4377-4380 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Kataoka: "Stereospecific reduction of ethyl 2'ーketopantothenate to ethyl Dー(ー)ーpantothenate with microbial cells as a catalyst" Appl.Environ.Microbiol.56. 3595-3597 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Hata et al.: "Ketopantoyl-lactone reductase from Candida parapsilosis : purification and characterization as a conjugated polyketone reductase" Biochem. Biophys. Acta. 990. 175-181 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Hata et al.: "Ketopantoyl lactone reducatse is a conjugated polyketone reductase" FEMS Microbiol. Lett.58. 87-90 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kataoka et al.: "Novel enzymatic production of D-(-)-pantoyl lactone through the stereospecific reduction of ketopantoic acid" Agric. Biol. Chem.54. 177-182 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kataoka et al.: "Microbial production of chiral pantothenonitrile throgh stereospecific reduction of 2'-ketopantothenonitrile" Biotechnol. Lett.12. 357-360 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Hata et al.: "Stereoselective reduction of diketones by a novel carbonyl reductase from Candida parapsilosis" J. Org. Chem.55. 4377-4380 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kataoka et al.: "Stereospecific reduction of ethyl 2'-ketopantothenate eith microbial cells as a catalyst" Appl. Environ. Microbiol.56. 3595-3597 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroyuki Hata: "Ketopantoylーlactone reductase from Candida parapsilosis;purification and characterization as a conjugated polyketone reductase" Biochim.Biophys.Acta. 990. 175-181 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroyuki Hata: "Ketopantoyl lactone reducatse is a conjugated polyketone reducatse" FEMS Microbiol.Lett.58. 87-90 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Michihiko Kataoka: "Novel enzymatic production of Dー(ー)ーpantoyl lactone through the stereopecific reduction of ketopantoic acid" Agric.Biol.Chem.54. 177-182 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Michihiko Kataoka: "Microbial production of chiral pantothenonitrile through stereospecific reduction of 2'ーketopantothenonitrile" Biotechnol.Lett.12. 357-360 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroyuki Hata: "Stereoselective reduction of diketones by a novel carbonyl reductase from Candida parapsilosis" J.Org.Chem.55. 4377-4380 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Michihiko Kataoka: "Stereospecific reduction of ethyl 2'ーketopantothenate to ethyl Dー(ー)ーpantothenate with microbial cells as a catalyst" Appl.Environ.Microbiol.56. 3595-3597 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroyuki Hata: "Ketopantoyl-lactone reductase from Candida parapsilosis:purification and characterization as a conjugated polyketone reductase" Biochim.Biophys.Acta. 990. 175-181 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiroyuki Hata: "Ketopantoyl lactone reductase is a conjugated polyketone reductase" FEMS Microbiol.Lett.58. 87-90 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Michihiko Kataoka: "Novel enzymatic production of D-(-)-pantoyl lactone throughthe stereospecific reduction of ketopantoyl lactone" Agric.Biol.Chem. 54. 177-182 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-03-31   Modified: 2016-04-21  

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