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A Study of Molecular Population Genetics on the Aspect of Edaptive evolution in Drosphila

Research Project

Project/Area Number 01480004
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionKyushu University

Principal Investigator

HARADA Ko (1990)  Kyushu Univ, Biology, Research Associate, 理学部, 助手 (40150396)

向井 輝美 (1989)  九州大学, 理学部, 教授 (30091242)

Co-Investigator(Kenkyū-buntansha) TAKANO Toshiyuki  Kyushu Univ. Biology, Research Associate, 理学部, 助手 (90202150)
YAMAZAKI Tuneyuki  Kyushu Univ. Biology, Associate Professor, 理学部, 助教授 (10108649)
日下部 眞一  広島大学, 総合科学部, 助教授 (40153275)
原田 光  九州大学, 理学部, 助手 (40150396)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1990: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsDNA polymorphism / molecular evolution / Drosophila / Transpasable element / Transposition rate / P element / 生存に有利な突然変異 / 遺伝子重複 / 遺伝子混成 / 調節遺伝子 / 非翻訳領域 / 淘汰的制約 / 自然選択 / 適応進化
Research Abstract

The generality of the neutral theory of molecular evolution (Kimura, 1968) has been accepted in the course of accumulation of molecular date of genes. Mukai and his colleagues have shown that most of the protein polymorphisms are selectively neutral. The purpose of this study is to investigate the genetic variation, that is the source of adaptive evolution, in molecular level and testify whether the neutral theory is operational for these experimental data.
Following things have become clear in this study. 1. We have shown that there is north to south cline in the additive genetic variance in viability. This is suggesting some of balancing selection is operating on these variations population. We studied restriction enzyme in southern polymorphism in four gene regions (Adh, Amy, Gpdh, Punch) in north (Amori) and south (Ogasawara) populations. There is no significant difference in restriction site polymorphisms, but significant difference was detected in the polymorphisms in insertions a … More nd deletions. Large amount of difference was observed in the southern population. This fact may suggest that excess additive genetic variance in the northern population is reflecting the cumulative effect of these variations. 2. The duplication or triplication of Gpdh gene is found to be polymorphic. It is pointed that gene duplication is important mechanism of gene evolution. These variation appeared to be maintained in the balance between mutational occurrence and purifying selection or by random genetic drift. Furthermore, we obtained an evidence that these genes evolved in concerted fashion by unequal crossing-over. 3. Transposable elements occupy about 10% of Drosophila genome and they often create complicated mutations. Transposition rate is one of the important parameter in evaluating the contribution of these mutations to adaptive evolution. Using mutation accumulation lines, we estimated transposition rates of several transposable elements. This the first reliable of such estimations. 4. We compared the distribution of several transposable elements in southern and northern populations. Some copia like elements shows difference in number between two populations. 5. We investigated the population structure concerning to P element activity and molecular structure. We obtained an evidence that in the course of invasion of P element, it causes large amount of genetic loads. There is variation in the length of P element. There is interpopulational difference in the distribution of complete size elements, but the frequency of KP like elements is increasing in all the populations investigated. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Takano,T.,Kusakabe,Koga,A.&Mukai,T.: "Poly morphism for the number of tandemly multiplicated glycerolー3ーphosphate dehydrogenase genes in Drosophila melanogaster." Proc Natel.Acad Sci.USA. 86. 5000-5004 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kusakabe,S.,Takano,T.& Mukai,T.: "Drilting Polymorphism of the number of multiplication at the glycerolー3ーphosphate dehydrogenase locus in notural populations of Drosophila melanogaster." Proc.Japnan.Acad.Ser.B. 165. 153-155 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kusakabe,S.,Baba,M.,Kogu,A.,Bewley,G.C.& Makai,T.: "Gene duplication and concerted evolution of the GPDH locus in natural populations of Drosophila melanogaster," Proc.Roy.Soc.Lond.Ser.B. 242. 157-162 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Harada,K.,Yukuhiro,K.,& Mukai,T: "Transpotition rates of movable elements in Drosophila melanogaster." Proc.Natl.Acad Sci.USA. 87. 3248-3252 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kusakabe,S.,Harada,K.& Mukai,T.: "The rare inversion with a Pelement at the braakpoint maintained in a natural population of Drasophila melanogaster." Genetica. 82. 111-115 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nitasaka,E.& Yamazaki,T.: "The relationship between DNA structural variations and activities of Pelements in typical Pana Q strains of Drosophila meranogaster." Genet.Res.Camb.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takano, T., Kusakabe, S., Koga, A. and Mukai, T.: "Polymorphism for the number of tandemly multiplicated glycerol-3-phosphate dehydrogenase genes in Drosophila melanogaster." Proc. Natl. Acad. Sci. USA 86. 5000-5004 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kusakabe, S. Takano, T. and Mukai, T.: "Drifting polymorphism of the number of multiplication at the glycerol-3-phosphate dehydrogenase locus in naturapopulations of Drosophila melanogaster." Proc. Japan Acad. Ser. B. 165. 153-155 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kusakabe, S., Baba, M., Koga, A. Bewley, G. C. and Mukai,: "Gene duplication and concerted evolution of the GPDH locus in natural populations of Drosophila melanogaster." Proc. Roy. Soc. Lond. Ser. B. 242. 157-162 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Harada, K., Yukuhiro, K. and Mukai, T.: "Transposition rates of movable genetic elements in Drosophila melanogaster." Proc. Natl. Acad. Sci. USA 87. 3248-3252 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kusakabe, S., Harada, K. and Mukai, T.: "The rare inversion with a P element at the breakpoint maintained in a natural population of Drosophila melanogaster." Genetica. 82. 111-115 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nitasaka, E. and Yamazaki, T.: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet. Res. Camb.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Harada,K.,Yukuhiro,K.& Mukai,T.: "Transposition rates of movable qenetic element in <Drosophila>___ー <melanogaster>___ー" Proc.Natl.Acad.Sci.USA. 87. 3248-3252 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mukai,T.,Harada,K.,Kusakabe,S.& Yamazaki,T.: "Estimation of spontaneous mutation rates at enzymeloci in <Drosophila>___ー <melanogaster>___ー" Proc.Japan Acad.Ser.B. 66. 29-32 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kusakabe,S.,Harada,K.,& Mukai,T.: "The rare inversion with a pelement at the break point maintained in a natural population of <Drosophila>___ー <melanogaster>___ー" Genetica. 82. 111-115 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Harada,K.,Kusakabe,S.& Mukai,T.: "Comparative study of the Pelement activity in two natural populations of <Drosophila>___ー <melanogaster>___ー" Genetica. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takano,T.,Kusakabe,S.& Mukai,T.: "The genefic structure of natural populations of <Drosophila>___ー <melanogaster>___ー.XXII.Comparative study of DNA polymorphisms in the northern and southern natural populations" Genetics. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nitasaka,E.& Yamazaki,T.: "The relationship between DNA structural variations and activities of Pelements in typical pand Q strains of <Drosophila>___ー <melanogaster>___ー" Genet.Res.Camb.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kusakabe,S.: "Drifting Polymorphism of the Number of Multiplication at the Glycerol-3-phosphate Dehydrogenase Locus in Natural Populations of D.melanogaster." Proceedings of the Japan Academy. 65. 153-155 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tkano,T.: "Polymorphism for the Number of Tandemly Multiplicated Glycerol-3-phosphate Dehydrogenase Genes in D." Proceedings of the National Academy of Sciences(USA).80. 5000-5004 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Mukai,T.: "Viability Polygenes in Populations of Drosophila melanogaster." Popilation Biology of Genes and Molevules.ed.by N.Takahata and J.F.Crow. 199-217 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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