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Regulation of plant expression by DNA methylation

Research Project

Project/Area Number 01480016
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionAkita Prefectural College of Agriculture

Principal Investigator

SANO Hiroshi  Biotechnology Institute, Molecular Genetics, Professor, 附属生物工学研究所, 教授 (20178809)

Co-Investigator(Kenkyū-buntansha) YOUSSEFIAN S.  Biotechnology Institute, Molecular Genetics, Lecturer, 講師 (00210576)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsDNA methylation / dwarfism / small GTP-binding protein / ras-related gene / Oryza sativa / azacytidine / rgp1 / transgenic tobacco / DNAメチル化 / rasー関連遺伝子 / 矮性植物 / GTP結合蛋白質 / rga1 / 矮性イネ / フザシチジン
Research Abstract

Exposure of normal, tall rice (Oryza sativa) seedlings to 5-azacytidine, a powerful inhibitor of DNA methylation in vivo, induced both demethylation of genomic DNA and dwarf plants. Genes that had been affected by treatment were identified by differential screening of a DNA library, and a gene encoding a small GTP-binding protein, rgp1, was subsequently. The cDNA or rgp1 was found to encode a deduced protein of 226 amino acids (24.8kDa) with highest sequence similarity to the ras-related ypt3 protein of fission yeast. The rgp1 protein, rgp1-p25, expressed in transformed E.coli, clearly showed GTP-binding activity. To determine the physiological role of rgp1-p25, the coding region of rgp1 was introduced into tobacco plants in both sense and anti-sense orientations. Transformants, with rgp1 in either orientation, showed distinct phenotypic changes; reduced apical dominance, dwarfism and abnormal flower development. The expression of tgp1, a tobacco homologue of rgp1, was markedly reduced in anti-sense transformants, indicating that tha phenotypic changes may have been mediated by interference with tgp1, mRNA. In sense transformants, the changes may have been induced by over-production of rgp1-p25. We speculated that the increased tillering may be related to abnormal phytohormone metabolism or response pathways. A preliminaly experiment clearly showed that the content of zeation, one of the active cytokinins in higher plants, differed significantly between transformants and controls. Furthermore, transformants were found to respond differentially to externally-applied cytokinins. These observations indicate that the rgp1-p25 may be involved in either the regulation of cytokinin biosynthesis or, more probably, in its intracellular transport.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Sano,H.,Youssefian,S.: "A novel ras-related rgpl gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice." Mol.Gen.Genet.228. 227-232 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamada,I.,Yamauchi,S.,Youssefian,S.,Sano,H.: "Transgenic tobacco plants expressing rgpl,a gene encoding a ras-related GTP-binding protein from rice,show distinct morphological characteristics." The Plant J.2. 799-807 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Youssefian,S.,Nakamura,M.,Sano,H.: "Molecular characterization of rgp2,a gene encoding a small GTP-binding protein from rice." Mol.Gen.Genet.237. 187-192 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐野 浩: "DNAメチル化による矮性発現の制御" 化学と生物. 29. 400-405 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐野 浩: "植物生長における低分子量GTP結合蛋白質の役割" 植物の化学調節. 27. 136-144 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐野 浩: "情報伝達とGタンパク質" 植物細胞工学. 5. 43-49 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sano, H. Youssefian, S.: "A novel ras-related rgplencoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice." Mol.Gen.Genet.228. 227-232 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamada, I. Yamauchi, S. Youssefian, S. Sano, H.: "Transgenic tobacco plants expressing rgp1, a gene encoding a ras-related GTP-binding protein from rice, show distinct morphological characteristics." The plant J.2. 799-807 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Youssefian, S. Nakamura, M. Sano, H.: "Molecular characterization of rgp2, a gene encoding a small GTP-binding protein from rice." Mol. Gen. Genet.237. 187-192 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroshi Sano,Ikuko Kamada,Shohab Youssefian,Masayuki Katsumi,and Hiroetsu Wabiko: "A single treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and undermethylation of genomic DNA" Molecular and General Genetics. 220. 441-447 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroshi Sano and Shohab Youssefian: "A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice" Molecular and General Genetics. 228. 227-232 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ikuko Kamada,Seiko Yamauci,Shohab Youssefian and Hiroshi Sano: "Transgenic tobacco plants expressing rgp1,a gene encoding a rasrelated GTP-binding protein from ricd,show distinct morphological characteristics" Plant J.2. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐野 浩: "DNAメチル化による矮性発現の制御" 化学と生物. 29. 400-405 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐野 浩: "高等植物の情報伝達系におけるGTP結合蛋白質の関与" 東北大学遺伝生態センタ-通信. 15. 8-12 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sano,H.etac.: "A novel rasーrelated <rgpl>___ー gene encoding a GTPーbinding protein has reduced expression in 5ーazacytidineーinduced dwarf rice" Mol.Gen.Genet.

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐野 浩: "DNAのメチル化と矮性発現" 化学と生物. 29. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sano,H.,et al.: "A single treatment of rice seedlings with 5azaーcytidine induces Genritable dwarfism and undermethylation of genomic DNA" Mol.Gen,Genet.220. 441-447 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2021-04-07  

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