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Molecular Mechanism of Long Distance Flight in Insects.

Research Project

Project/Area Number 01480020
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionInstitute of Low Temperature Science, Hokkaido University

Principal Investigator

CHINO Haruo  (Professor), 低温科学研究所, 教授 (00012253)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1989: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsLong distance flight / Lipophorin / Adipokinetic hormone / Diacylglycerol / Apolipophorin-III / Fat body / Trehalose / Calcium ion / トノサマバッタ / ゴキブリ / グリセリドプ-ル / アポリポホリンーIII / アポリポホリン-III / アディポキネティックホルモン / Caイオン
Research Abstract

The major results are summarized as follows :
1. The primary action of adipokinetic hormone (AKH) is to stimulate the influx of extracellular calcium ion into the fat body cells which results in the activation of lipase via calcium-dependent protein kinase. Consequently, the formation of diacylglycerol from triacylglycerol is accelefated, which, in turn, promotes loading of diacyllgycerol by lipophporin from the fat body.
2. Low density lipophorin (LDLp) induced by AKH associates a different number of apolipophorin-III (apo-III), 7moles/ lipophorin to 14 moles/lipophorin, in accordance with the amount of diacylglycerol loaded by lipophorin, and most of LDLp contain about 9 moles apo-III per each mole of lipophorin. In addition, It was confirmed that the heterogeneity in size of LDLp particles previously observed is undoubtedly due to the intermolecular fusion of LDLp particles primarily formed by the action of AKH.
3. The insect blood sugar, trehalose, inhibits the loading of diacylglycerol by lipophorin from the fat body. This inhibition occurs at the physiological concentration of trehalose, about 60-80 mM ; however, at the commencement of long distance flight, the trehalose level sharply drops to 20 mM during the first 30 min, at which concentration no more inhibition takes place. This suggests that trehalose may play a dual function, i. e., serving as fuel and as a regulator in lipid transport.
4. Locust (as an insect having capacity of long distance flight) and cockroach (as an insect lacking the capacity) are compared. It was found that cockroach hemolymph lacks apolipophorin-III, and that cockroach fat body contains extremely small amount of diacylglycerol, which is equivalent to only one tenth of diacylglycerol content in locust fat body.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 平山 雄二・茅野 春雄: "Lipid transfer particle of locust hemolymph:Purification and characterization." Journal of Lipid Research. 31. 793-800 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] ラム・ペッキ-・茅野 春雄: "Trehalos,the insect blood sugar,inhibits loading of diacylglycerol by lipophorin from the fat body in locusts." Biochemca Biophysica Research Communication. 172. 588-594 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] ラム・ペッキ-・茅野 春雄: "Primary rloe of adipokintec in the formation of low density lipophorin in locusts." Journal of Lipid Research. 31. 2039-2044 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 長尾 恵理子・茅野 春雄: "Further Chracterization of low density lipophorin inducaed by adipokinetic hormone in locusts." Journal of Lipid Research. 32. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirayama, Y., and Chino, H.: "Lipid transfer particle of locust hemolymph : Purification and characterization." J. Lipid Research,. 31,. 793-800 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Lum, P. Y. and Chino, H.: "Trehalose, the insect blood sugar, inhibits loading of diacylglycerol by lipophorin from the fat body in locusts." Biochem. Biophys. Res. Commu.172,. 588-594 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Lum P. Y. and Chino, H.: "Primary role of adipokinetic hormone in the formation of low density lipophorin in locusts." J. Lipid Research. 31,. 2039-2044 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagao, E. and Chino, H.: "Further characterization of low density lipophorin induced by adipokinetic hormone in locusts." J. Lipid Research,.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 平山 雄二,茅野 春雄: "Lipid transfer particle in locust hemolymph:Purification and characterization." Journal of Lipid Research. 31. 793-800 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] ラム・ペッキ-,茅野 春雄: "Primary role of adipokinetic hormone in the formation of low density lipophorin in locusts." Journal of Lipid Research. 31. 2039-2044 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] ラム・ペッキ-,茅野 春雄: "Trehalose,the insect blood sugar,inhibits loading of diacylglycerol by lipophorin from the fat body in locusts" Biochem.Biophys.Res.Commun.172. 588-594 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 長尾 恵理子,茅野 春雄: "Further charactrization of low density lipophorin induced by adipokinetic hormone in locusts." Journal of Lipid Research. 32. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 茅野 春雄,ラム・ペッキ-,長尾 恵理子,平岡 毅: "Bioenergetic essentials for long distance flight in insects." Journal of Comparative Physiology.

    • Related Report
      1990 Annual Research Report
  • [Publications] 茅野春雄,平山雄二: "Lipid transfer particle of locust hemolymph" Journal of Lipid Research. 31. 5 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] ラム・ペッキィ-,茅野春雄: "The primary role of adipokintic hormone in the formation of low density lipophorin in locusts." Journal of Lipid Research.

    • Related Report
      1989 Annual Research Report
  • [Publications] 長尾恵理子,茅野春雄: "Further characterization of low density lipophorin induced by adipokinetic hormone in locusts." Journal of Lipid Research.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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