| Project/Area Number |
01480023
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Tohoku University |
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Principal Investigator |
IDE Hiroyuki Tohoku Univ., Fac. of Science, Assoc, Prof., 理学部, 助教授 (70022704)
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| Co-Investigator(Kenkyū-buntansha) |
OHSUGI Kojune Tohoku Univ., Fac. of Science, Ass. Prof., 理学部, 助手 (00201377)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | cartilage pattern / retinoic acid / molecular mechanism / 肢芽 / パタ-ン形成 / 軟骨分化 |
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| Research Abstract |
To elucidate the molecular mechanisms of cartilage pattern formation of chick limb bud, retinoic acid (RA), which was known to induce limb duplication, was locally applied to the various regions of the limb bud or added to the limb mesodermal cells in culture. RA applied to the antero-distal region of the limb bud induced ZPA activity and stimulated the expression of RARbeta mRNA. The induction of ZPA activity was independently of duplicate formation and depended on the presence of AER at the distal top of the limb bud.
RA induced the formation of 18kD protein in limb bud cell culture. This induction occurred only in the cells of anterior and distal region. Induction of ZPA activity in cell culture also occurred only in these cells, suggesting the role of 18kD protein in ZPA induction.
RA promotes the proliferation and chondrogenesis in cultured distal mesodermal cells of limb bud. This promotion also occurred in the cells of antero-distal region.
These results suggest a common mechanism in the induction of ZPA activity in vivo and in vitro and the promotion of cell proliferation and chondrogenesis by RA. .
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