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Molecular Mechanisms of Muscle Cell Differentiation in Embryos Ascidian

Research Project

Project/Area Number 01480027
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionKyoto University

Principal Investigator

SATOH Noriyuki  Kyoto Univ., Fac. Sci., Assoc. Prof., 理学部, 助教授 (30025481)

Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsCell differentiation / Myosin heavy chain gene / Muscle actin genes / MyoD-like gene / Determinants / Cis-regulatory elements / Ascidian embryos / 筋肉細胞分化 / アクチン遺伝子 / MyoD様遺伝子 / 卵細胞質決定因子 / ホヤ / 分子メカニズム / マイオプラズミン
Research Abstract

Molecular mechanisms of muscle cell differentiation of ascidian embryos have been investigated with respect to an extreme potential of autonomous differentiation of the cells.
First, we have isolated CDNA clones for myosin-heavy chain and museletype actins. Expression of these muscle-specific genes beans around the time of gastrulation, and is restricted to differentiating sele cells. Ascidian embryos synthesize several different actin mRNAs. One of the clones, HrcMA4 contains an open reading frame of 1137 bp and a 100 bp noncoding region followed by a poly(A)tail. Then, we have isolated genomic fragments that contain HrMA4 gene. Characterization of the gene shows that HrMA4 is interrupted by two introns. There are several potential regulatory elements for accurate expression of the gene. In addition, it becomes clear that HrMA4 and several other muscle-actin genes form a cluster.
Second, we have tried to isolate genes for myogenic factors such as MyoDl and myogenic. S6 far, we have obtained by PCR method a candidate DNA-fragment for the genes.
Finally, we have analyzed macromolecules of the myoplasm, which is thought to contain the myogenic factors, by producing several monoclonal antibodies. One of the antibodies blocks muscle development when it is injected into fertilized eggs. Characterization of the antigenic polypeptide, however, has shown that it is related to cytoskeletal proteins.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Satoh,N.: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos" Intern.Review Cytology. 122. 221-258 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makabe,K.W.: "Specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryos" Roux's Arch.Develop.Biol.199. 307-313 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kusakabe,T.: "Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi" Develop.Growth Differ.33. 227-234 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Marikawa,Y.: "Gray and red fragments of the egg of the ascidian Ciona savignyi:Preferential development of muscle cells from gray fragments" Develop.Growth Differ.33. 307-316 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makabe,K.W.: "Failure of muscle myosin heavy-chain gene expression in quarter ascidian embryos developed from the secondary muscle lineage cells" Zool.Sci.9. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kusakabe,T.: "Tunicate muscle actin genes structure and organization as a gene cluster"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoh,N.: "Developmental Biology" Wiley-Liss,Inc., 388 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoh,N.: "Frontiers in Muscle Research" Excerpta Medica, 450 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoh, N.: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos" Intern. Review Cytology. 122. 221-258 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makabe, K. W.: "Specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryos" Roux's Arch. Develop. Biol.199. 307-313 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kusakabe, T.: "Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi" Develop. Growth Differ.33. 227-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Marikawa, Y.: "Gray and red fragments of the egg of the ascidian Ciona saving : Preferential development of muscle cells from gray fragments" Develop. Growth Differ.33. 307-316 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makabe, K. W.: "Failure of muscle myosin heavy-chain gene expression in quarter ascidian embryos developed from the secondary muscle lineage cells" Zool. Sci.9. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kusakabe, T.: "Tunicate muscle actin genes structure and organization as a gene cluster"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoh, N.: "Developmental Biology" Wiley-Liss, Inc.143-151 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoh, N.: "Frontiers in Muscle Research" Excerpta Medica. 169-178 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Marikawa,Y.: "Gray and red fragments of the egg of the ascidian Ciona savignyi:preferential development of muscle cells from gray fragments" Develop.Growth Differ.33. 307-316 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kusakabe,T.: "Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi" Develop.Growth Differ.33. 227-234 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kusakabe,T.: "Organization and structure of muscle actin genes of the ascidian Halocynthia roretzi"

    • Related Report
      1991 Annual Research Report
  • [Publications] Araki,I.: "Chacterization of a protochordate muscle myosin heavyーchain gene:Complete nucleotide and protein coding sequence"

    • Related Report
      1991 Annual Research Report
  • [Publications] Satoh,N.: "Frontiers in Muscle Research" Excerpta Medica, 450 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 矩行: "発生・分化の遺伝子的背景" 東京大学出版会, 288 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Satoh,Noriyuki: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos" International Review of Cytology. 122. 221-258 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Makabe,Kazuhiro W.: "Specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryo" Roux's Arch.Developmental Biology. 199. 307-313 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kusakabe,Takehiro: "Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi" Development,Growth & Differentiation. 33. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Satoh,Noriyuki: "Expression of muscle myosin heavy chain gene in quarter ascidian embryos" Journal of Experimental Zoology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Marikawa,Yusuke: "Gray and red fragments of the egg of the ascidian Ciona savignyi:Preferential developemnt of muscle cells from gray fragments" Development,Growth & Differentiation. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Satoh,Noriyuki (Eds.)E.H.Davidson et al.: "Developmental Biology" WileyーLiss,New York, 388 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Satoh,Noriyuki (Ed.)G.M.Malacinski: "Cytoplasmic Orgamization System" McGrawーHill Publ.Co.,New York, 482 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuhiro W.MAKABE: "Temporal expression of myosin heavy chain gene during ascidian embryogenesis" Develop.Growth Differ.31. 71-77 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Noriyuki SATOH: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos" International Review of Cytology. 122. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Noriyuki SATOH: "DEVELOPMENTAL BIOLOGY(UCLA Symposia,vol 125)(eds.E.Davidson et al)" Alan R.Liss,Inc.,New York, 500 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Noriyuki SATOH: "CYTOPLASMIC ORGANIZATION SYSTEMS(ed.G.M.Malacinski)" McGrow-Hill Publ.Co.,New York, 477 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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