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Regulation of vitellogenesis in ticks and purification of vitellogenesis inducing factor.

Research Project

Project/Area Number 01480052
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionMie University

Principal Investigator

CHINZEI Yasuo  Mie University, School of Medicine Professor, 医学部, 教授 (60024709)

Co-Investigator(Kenkyū-buntansha) MIURA Ken  Mie University School of Medicine Assistant, 医学部, 助手 (60219582)
MATSUOKA Hiroyuki  Mie University School of Medicine Lecturer, 医学部, 講師 (10173816)
ANDO Katsuhiko  Mie University School of Medicine Associate Professor, 医学部, 助教授 (90024710)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordstick / Ornithodoros / vitellogenin / vitellin / juvenile hormone / precocene / synganglion / cypermethrin / 卵黄形成 / Ornithodoros
Research Abstract

Biosynthesis of yolk protein and its hormonal regulation were investigated in the soft tick, Ornithodoros mouvata and Ornithodoros parkeri. We first purified and characterized yolkk protein, vitellin (Vn), and the hemolymph precursor of Vn, vitellogenein (Vg) from newly laid eggs and hemolymph of the tick, respectively. we demonstrated Vn and Vg are a conjugated phospho-glycoprotein associated non-covalently with haem and lipid, which molecular size is about 600 kD as native and is composed 6 polypeptides. Vg is synthesized in the fat body, secreted into hemolymph and accumulated into the developing oocytes as Vn. Vn is composed of 6 polypeptides differnt (more smaller) from that of Vg. Vg synthesis in the fat body is primarily activated by the stimulation of engorgement. After blood meal Vg appear in the hemolymph first on day 2 after engorgement and increased to very high concentration in the hemolymph (maximum 60, mugl, mul). We have tried to produce a hormone free ticks by using pr … More ecocene to study hormonal induction of Vg synthesis. We could- not produce those animals successfully, but 'we knew that precocene has no effect in lower doses (less than 100 mug/tick) and toxic in higher doses (more than 150 mug/tick) in this species of tick. But we found that one of synthetic pyrethroid, cypermethrin (CyM) has a positive effect to induce Vg synthesis and ovarian development in unengorged (both mated and virgin) adult females, and also even unengorged males and nymphs. CyM treated unengorged females produced Vg and developed ovary (but fewer eggs). This meant that unengorged females have ability to produce Vg if given a proper humoral conditions. We applied several natural juvenile hormones (JH) and JH analogs (JHA) on unengorged females, but we could not get positive effects for vitellogenesis. Therefore we concluded JH is not involved in the course of Vg synthesis induction after engorgement. From the fact that nerve activating pyrethroid, CyM, can induce vitelogenin synthesis, and from some ligation experiments we showed synganglion (SyG) has a factor which induces Vg synthesis in the fat body. We showed unengorged females treated with extract of synganglions collected from unengorged females produced Vg in significantly higher concentration than tick Ringer treated animals. We tried to purify this vitellogenesis inducing factor (VIF) and showed VIF is a polypeptids. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Yasuo Chinzei,Kunio Ito and Katsukiko Ando: "Cypermethrin in duction of uitellogenesis and ouarian deuelopment in unfed adult female,Ornithodoros moubata." Inuertebrate Reprodu Elion and Development. 15. 19-26 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Demar Taycor,Yasuo Chiuzei and Katsuhiko Ando: "Stimulation of vitellogenesis by pyrethroids in mated and uirgin female adults,male abults,and 4th instar females of Ornithodorus moubata (Acari:Argasidad)." Journal of Medical Entomology. 28. 322-329 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei,Demar Taylor and Katsuhico Ando: "Effects of juvenile hormone and its analogs on uitellogenin synthesis and ouarian denelopment in Ornithoduros moubata(Acari:Argasudae)." Journal of Medical Entomology. 28. 506-513 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] DeMar Taylor,Yasuo Chinzei Ken Miura and Katsuhiko Ando: "Vitellogenin synthesis,processing and ho/monal regulation in the hck,Ornithodoros parkeri(Acari:Argasidae)." Insect Biochemistry. 21. 723-733 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chiza,Demar Taylor Ken Miura and Katsuhiko Ando: "Vitellogenesis induction by synganglion Factrr in adult Female tick,Ornithodoros moibata (Acari:Argasidae)." Acarological Science. 1. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Demar Taylor,Yasuo Chinzei Ken Miura and Katsuhiko Ando: "Effects of precocene on uitellogenesis in the adult female tick,Ornithodoros moubata (Acari:Argasidae)" Experimental Applied Acarology. 10. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei and DeMar Taylor: "Aduances in Invertebrate Reproduction Vol5(ed.by Hoshi and Yamashita)" Elsenier Science Publi cations, 570 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei and DeMar Taylor: "Advances in Disease Vector Research" Springer-Verlag, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei, Kunio Ito and Katsuhiko Ando: "Cypermethrin induction of vitellogenesis and ovarian development in unfed adult female Ornithodoros moubata (Acari : Argasidae)." Invertebrate Reproduct. Develop.15. 19-26 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo chinzei and DeMar Taylor: "Regulation of vitellogenesis induction by engorgement in the soft tick, Ornithodoros moubata." "Advances in Invertebrate Reproduction"Vol, 5. Elsevier Science Publications B. V. (Biochemical Division). 565-570 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] DeMar Taylor, Yasuo Chinzei and Katsuhiko Ando: "Stimulation of vitellogenesis by pyrethroids in mated and virgin female adults, males adults, and 4th instar females of Ornithodoros moubata (Acari : Argasidae)" J. Med. Entomol.28. 322-329 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei, DeMar Taylor and Katsuhiko Ando: "Effects of juvenile hormone and its analogs on vitellogenin synthesis and ovarian development in Ornithodoros moubata (Acari : Argasidae)." J. Med. Entomol.28. 506-513 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] DeMar Taylor, Yasuo Chinzei, Ken Miura and Katsuhiko Ando: "Vitellogenin synthesis, processing and hormonal regulation in the tick, Ornithodoros parkeri (Acari : Argasidae)." Insect Biochem.21. 723-733 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] DeMar Taylor, Yasuo Chinzei, Ken Miura and Katsuhiko Ando: "Effects of precocene on vitellogenesis in the adult females tick, Ornithodoros moubata (Acari : Argasidae)." Experimentl. Appl. Acarol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei, DeMar Taylor, Ken Miura and Katsuhiko Ando: "Vitellogenesis induction by synganglion factor in adult female tick, Ornithodoros moubata (Acari : Argasidae)." Acarological Science. 1. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei and DeMar Taylor: "Vitellogenesis in ticks : Vitellogenin and regulation of vitellogenin synthesis induction by engorgement." "Advances in Disease Vector Research"Vol. 9. Springer-Verlag.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasuo Chinzei,Kunis Ito and Katsuhiko Ando: "Cypermethrin in duction of uitellogenesis and ovarian deuelopment in unfed female,Ornithodoros moubata." In vertebrate Reproduction and Deuelopment. 15. 19-26 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] DeHar Taylor,Yasuo Chinzei and Katsuhiko Ando: "Stimulation of vitellogenesis by pysethsoids in mated and virgin female adults,and 4th instar females of Ornithodorus moubato(Acadi:Argasidae)." Journal of Medical Entomology. 28. 322-329 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yasuo Chinzei,DeMar Taylor and Katsuhico Ando: "Effects of juvenile hormone and its analogs on vitellogiuin synthesis and ouarion development in Ornithodoros moubato(Acari:Argasidae)." Journal of Medical Entomology. 28. 506-513 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] DeMar Taylor,Yasuo Clinzei,Ken Miura and Katsuhiko Ando: "Vitellogenin synthesis,processing and Hormonal regulation in the tick,Ornithodoros parkeri(Acari:Argasidae)." Insect Biochemirtry. 21. 723-733 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] DeMar Taylor,Yasuo Clinzei,Ken Miura and Katsuhiko Ando: "Vitellogerasis induction by synganglion factor in adult fomale tick,Ormithodoros mocibata(Acari:Argasidae)." Acarological Science. 1. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] DeMar Taylor,Yasuo Clinzei,Ken Miura and Katsuhiko Ando: "Effeces of precocene on vitellogenesis in adult female tick,Ornithodoros moubata(Acari:Argasidae)" Experimental Applied Acarology. 10. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yasuo Chinzei and DeMar Taylor (ed.by Hoshi and Yamazhita): "Advances in Invertebrate Repsoduction Vol5" Elsevier Science Publicaltons, 570 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yasuo Chinzei and DeMar Taylor: "Advances in Disease Vector Reseasch" SpringerーVerlog, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yasuo Chinzei: "Vitellogenin synthesis and ovarian development in nymphal and newly molted female Ornithodoros moubata (Acari:Argasidae)" Journal of Medical Entomology. 26. 30-36 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuo Chinzei: "Cypermethrin induction of vitellogenesis and ovarian development in unfed adult females Ornithodoros monbata (Acari:Argasidae)." Invertebrate Reproduction and Development. 15. 19-26 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuo Chinzei: "Regulation of vitelloyenesis induction by engorgement in the soft tick,Ornithodoros moubata" Elsevier Science Publications:Invertebrate Reproduction. 5. 565-570 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Demar Taylor: "Induction of vitellogenesis by pyrethroids in mated and virgin female adults,and 4th instar female nymph of Ornithodoros moubata (Acari:Argasidae)." Journal of Medical Entomology. 28. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuo Chinzei: "Effects of juvenile hormone and juvenile hormone analog on induction of uitellogenesis in Ornithodoros moubata." Journal of Medical Entomology. 28. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuo Chinzei: "Vitellogenin Synthesis and Ovarian Development in Nymphal and Newly Molted Female Ornithodoros moubata(Acari:Argasidae)" Journal of Medical Entomology. 26. 30-36 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuo Chinzei: "Cypermethrin Induction of Vitellogenesis and Ovarian Development in Unfed Adult Female Ornithodoros moubata(Acari:Argsidae)" Invertebrate Reproduction and Development. 15. 19-26 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuo Chinzei: "Regulation of Vitellogenesis Induction by Engorgement in the Soft Tick,Ornithodoros moubata" Advances in Invertebrate Reproduction. 5. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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