Studies on structure and functions of silkworm proteins

• Project/Area Number: 01480056
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 蚕糸学
• Research Institution: University of Tokyo
• Principal Investigator: NAGATA Masao Fac. Agricul, Univ. Tokyo Associate Professor, 農学部, 助教授 (70107407)
• Co-Investigator(Kenkyū-buntansha): WATANABE Hitoshi Tokyo Agricul. Univ. Visiting Professor, 総合研究所, 客員教授 (10011868) ; SHIMADA Toru Fac. Agricul, Univ. Tokyo Assistant Professor, 農学部, 助手 (20202111) ; KOBAYASHI Masahiko Fac. Agricul, Univ. Tokyo Professor, 農学部, 教授 (60162020)
• Project Period (FY): 1989 – 1991
• Project Status: Completed (Fiscal Year 1991)
• Budget Amount: ¥7,100,000 (Direct Cost: ¥7,100,000); Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000); Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 1989: ¥6,000,000 (Direct Cost: ¥6,000,000)
• Keywords: Silkworm / Proteins / 血液タンパク質

Research Abstract

Insect haemolymph contained a large amount of proteins, which enormously varied with development. Many of these proteins have the same structure as a hexamer of subunits with about 80, 000 dalton, but their functions are different. There are two proteins belonging to a category of storage proteins in the silkworm, Bombyx mori. We investigated the structure and function of these storage proteins, arylphorin and larval female-specific protein. 1)Purification methods were improved to obtain a large amount of storage proteins. 2)Nature of storage proteins such as molecular weights of native proteins and subunits, isoelectric point, conjugated components were revealed. 3)Association, dissociation of subunits of storage proteins and agglutination of proteins under various conditions were analyzed. 4)Changes of protein metabolism and role of storage proteins during larval development were examined. 5)Relationship between storage protein concentrations and nutritional conditions of silkworm larvae was investigated.

Research Products

• [Publications] Nagata,M.and Yoshikake,N.: "Protein metabolism in the forval development of the silkworm,Bombyx mori:Protein reutilization at the fourth molt." 日本蚕糸学雑誌. 58. 221-233 (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagata,M.and Kobayashi,M.: "Quantitatine changes in storage proteins during larval debelopment of the silkworm,Bombyx mori" 日本蚕糸学雑誌. 59. 461-468 (1990)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagata,M.and Kobayashi,M.: "Effects of nutrition on Storage proteins concentrations in the larval hemolymph of the silkworm,Bombyx mori" 日本蚕糸学雑誌. 59. 469-474 (1990)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagata, M. and Yoshitake, N: "Protein metabolism in the larval development of the silkworm, Bombyx mori : Protein reutilization at the fourth molt." J. Seric. Sci. Jpn.58. 221-233 (1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagata, M. and Kobayashi, M.: "Quantitative changes in storage proteins during larval development of the silkworm, Bombyx mori." J. Seric. Sci. Jpn.59. 461-468 (1990)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagata, M. and Kobayashi, J.: "Effects of nutrition on storage proteins concentrations in the larval hemolymph of the silkworm, Bombyx mori." J. Seric. Sci. Jpn.59. 469-474 (1990)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagata,M.: "Quantitative changes in storage proteins during larval development of the silkworm,<Bombyx>___ー <mori>___ー" 日本蚕糸学雑誌. 59. 461-468 (1990)
• [Publications] Nagata,M.: "Effects of nutrition on storage protein concentrations in the larval hemolymph of the silkworm,<Bombyx>___ー <mori>___ー" 日本蚕糸学雑誌. 59. 469-474 (1990)
• [Publications] Nagata,M.: "Protein metabolism in the larval development of the silkworm ,Bombyx mori:Protein rentilization at the fourth molt" 日本蚕糸学雑誌. 58. 221-233 (1989)