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Structure Determination of a New Type of Taste-modifying Protein with Activity of Modifying a Sour Taste into Sweet Taste.

Research Project

Project/Area Number 01480066
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionYokohama National University

Principal Investigator

KURIHARA Yoshie  Yokohama Natl. Univ., Fac. of Education, Professor., 教育学部, 教授 (90017715)

Co-Investigator(Kenkyū-buntansha) NAGASE Shigeru  Yokohama Natl. Univ., Fac. of Education, Professor, 教育学部, 教授 (30134901)
麻生 武夫  横浜国立大学, 教育学部, 教授 (40017736)
浅見 真年  横浜国大, 教育学部, 助教授 (20134439)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordscurculin / sweet protein / taste-modifying protein / 味覚修飾物質
Research Abstract

A new taste-modifylng protein named curculin was extracted with 0.5 M NaCl solution from fruits of Curculiro atifolia and purified by ammonium sulfate fractionation, CM-Sepharose Ion-exchange chromatography, and gcl filtration. The purilied curculin thus obtained gave a single band having molecular welght of 12, 000 In sodium dodecyl sulfate-polyacrylamide gel electrophoresis In the presence of 8 M urea. The molecular weight determined by low-angle light scattcring was 27, 800. These results suggested that native curculin Is a dimer of 12, 000 dalton polypeptide. The complete amino acid sequence of curculin was determined by an automatic Edman degradation method. Curculin consists of 114 amino acid residues. Curculin itself elicits a sweet taste. After curculin, water elicits a sweet taste and sour substances induce stronger sweetness. No protein carrying both a sweet-tasting activity and taste-modifying activitics has ever been known. There were 5 sets of tripeptides common to miraculin (taste-modifying protein). 6 sets of tripeptides common to thaumatln (sweet protein) and 2 sets of tripeptides common to monellin (sweet protein). An enzymc immunoassay method for curculin was established. This method can accurately quantity 0.05-20 ng of curculin, sensitivity about 3000-times that of the psychometric method. Immunoblot analysis Indicated that antiserum to curculin was faintly reactive with miraculin, but not with thaumatin or moncllin. The mechanism of the taste-modifying actions of curculin were discussed.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 栗原 良枝: "甘味機能調節物質" 化学と生物. 29. 531-536 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 栗原 良枝: "みにれびゅう;甘味タンパク質および甘味誘導タンパク質" 生化学. 63. 1340-1345 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 栗原 良枝: "味を変える蛋白質一砂糖なしでも紅茶やレモンが甘くなる" 現代化学. 240. 28-33 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nakajho,et al.: "An enzyme immunoassay and immunoblot analysis for curculin,a new type of taste modifying protein:cross-reactivity of curculin and miraculin to both antibodies" Biochim.Biophys.Acta,. 1118. 293-297 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Igcta,et al.: "Determination of disulfide array and subunit structure of taste-modifying protein,miraculin" Biochim.Biophys.Acta.1079. 303-307 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Yamashita,et al: "Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity,curculin" J.Biol.Chem.265. 15770-15775 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Kurihara: "Characteristics of proteins with sweet and/or taste-modifying properties(Critical Reviews in Food Sciences and Nutrition," CRC PRESS, 25 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Yamashita, S. Theerasilp, T. Aiuchi, K. Nakaya, Y. Nakamura, and Y. Kurihara: "Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity, curculin." J. Biol. Chem.265. 15770-15775 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Takahashi, H. Hitotsuya, H. Hanzawa, Y. Arata and Y. Kurihara: "Structural study of asparagine-linked Oligosac charide moicty of taste-modifying protein, miraculin" J. Biol. Chem.265. 7793-7798 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Igeta, Y. Tamura, K. Nakaya, Y. Nakamura, and Y. Kurihara: "Determination of disulfide array and subunit structure of taste-modifying protein, miraculin" Biochem. Biophys. Acta.1079. 303-307 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Nakajho, T. Akabane, K. Nakaya, Y. Nakamur a, nad Y. Kurihara: "An enzyme immunoassay and immunoblot analysis for curculin, a new type of taste modifying protein : cross-reactivity of curculin and miraculin to both antibodies" Biochem. Biophys. Acta.1118. 293-297 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Abe, H. Yamashita, S. Arai and Y. Kurihara: "Molecular cloning of curculin, a novel taste-modifying protein with sweet taste" Biochem. Biophys. Acta. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Kurihara: "Characteristics of proteins with sweet and/or taste-modifying properties" Critical Reviews in Food Sciences and Nutriti. CRC Press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe,K.,et al.: "Molecular cloning of curculin,a novel tasteーmodifying protein with sweet taste" Biochim.Biophys.Acta. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nakajo,S.,et al.: "An enzyme immunoassay and immunoblot analysis for curculin,a new type of tasteーmodifying protein:Crossーreactivity of curculin and miraculin to both antibodies," Biochim.Biophys.Acta,. 1118. 293-297 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 栗原 良枝: "甘味タンパク質および甘味誘導タンパク質." 生化学. 63. 1340-1344 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Igeta,H.,et al.: "Determination of disulphide array and subunit structure of tasteーmodifying protein,miraculin," Biochim.Biophys.Acta,. 1079. 303-307 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamashita,H.,et al.: "Purification and complete amino acid sequence of a new type of sweet protein with tasteーmodifying activity,curculin," J.Biol.Chem.,. 265. 15770-15775 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Kurihara: "Characteristics of proteins with sweet and/or tasteーmodifying protein," Critical Reviews in Food Sciences and Nutrition(CRC Press),. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Yamashita et al.: "Purification and complete amino acid sequence of a new type of sweet protein with tasteーmodifying activity,curculin" J.Biol.Chem.265. 15770-15775 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Takahashi et al.: "Structural Study of Asparagineーlinked Oligosaccharide Moiety of Tasteーmodifying protein,Miraculin" J.Biol.Chem.265. 7793-7798 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Theerasilp et al.: "Complete Amino Acid Sequence and Structure Characterization of Tasteーmodifying Protein,Miraculin" J.Biol.Chem.264. 6655-6659 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Meada et al.: "Studies on the Taste Modifiers.II.Purification and Structure Determination of Gymnemic Acid,Antisweet Principle from Gymnema sylvestre Leaves" Tetrahedron Lett.30. 1547-1550 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Theerasilp et al.: "Complete Purification and Characterization of the Tasteーmodifying Protein,Miraculin,from Miracle Fruit" J.Biol.Chem.263. 11536-11539 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Nakajo et al.: "A Quantitative Enzyme Immunoassy for Miraculin in Miracle Fruit" Chemical Senses. 13. 663-669 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Takahashi et al: "Structural Study of Asparagine-linked Oligosaccharide Moiety of Taste-modifying protein,Miraculin" J.Biol.Chem.(in press). 265. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Theerasilp et al.: "Complete Amino Acid Sequence and Structure Characterization of Taste-modifying Protein,Miraculin" J.Biol.Chem.264. 6655-6659 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Meada et al.: "Studies on the Taste Modifiers.II.Purification and Structure Determination of Gymnemic Acid,Antisweet Principle from Gymnema sylvestre Leaves" Tetrahedron Lett.30. 1547-1550 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Theerasilp et al.: "Complete Purification and Characterization of the Taste-modifying Protein,Miraculin,from Miracle Fruit" J.Biol.Chem.263. 11536-11539 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Nakajo et al.: "A Quantitative Enzyme Immunoassy for Miraculin in Miracle Fruit" Chemical Senses. 13. 663-669 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Kurihara et al.: "Studies on the Taste Modifiers.I.Purification and Structure Determination of Sweetness Inhibiting Substance in Leaves of Ziziphus jujuba" Tetrahedron. 44. 61-66 (1988)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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