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Comparative Study of Prostaglandin Synthesis and Blood Coagulation in the Fish

Research Project

Project/Area Number 01480082
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionHiroshima University

Principal Investigator

KAYAMA Mitsu  Hiroshima University, Department of Applied Biological Science, Professor, 生物生産学部, 教授 (90034446)

Co-Investigator(Kenkyū-buntansha) IIJIMA Noriaki  Hiroshima University, Department of Applied Biological Science, Associate profe, 生物生産学部, 助教授 (90136143)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1990: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1989: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsFish / Thrombocytes / Prostaglandin / Gill / Monohydroxy fatty acid / GC-MS / 栓球 / モノヒドロキシ酸 / エラ
Research Abstract

Prostaglandin (PG) syntheses from the labelled highly unsaturated fatty acids were investigeted in washed thrombocytes suspensions of four species of marine fish, flounder, black sea bream, black rockfish, and red sea bream. When [1-^<14>C] -arachidonic acid (AA) was investigated with washed thrombocytes suspension, AA was mainly converted to PGF_<2alpha>, PGE_2, PGD_2 in all species. It was suggested that [1-^<14>C] eicosapentaenoic acid was mainly converted into PGs only in black rockfish thrombocytes, and the metabolites were mainly PGE_3 and PGD_3. However, [1-^<14>C] docosahexaenoic acid was not cyclized into PGs in all species. Aggragation behaviors of human platelets and carp, rainbow trout, and red sea bream thrombocytes were studied by an microscopic observation. Human platelets and all fish thrombocytes aggregated in the presence of ionophore A_<23187>, thrombin, and collagen, but did not aggregate in the presence of EPA, DHA, PGF_2, PGD_2 or ADP. Moreover, fish thrombocytes did not aggregate in the presence of TXA_2, PGG_2 , and PGH_2 whose induces the aggragation of human platelets. It was recognized that the inducers of thrombocytes aggregation appeared in the supernatant of carp thrombocytes suspension which aggregated by collagen. However, the structure of the inducers could not be determined.
The endogeneous products extracted from the gill of red sea bream were isolated by high-performance liquid chromatography and identified as 12-hydroxyeicosatetraenoic acid, 12-hydroxyeicosapentaenoic acid, and 14-hydroxydocosahexaenoic by ultraviolet absorption spectra and gas chromatography-mass spectrometry. The endogeneous products of carp gill were also identified as 13-hydroxyoctadecadienoic acid, 12-hydroxyeicosatetraenoic acid, and 12-hydroxyeicosapentaenoic acid.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] M.Kayama,N.Iijima,T.Sado,H.Matsumoto,and T.Hada: "Prostaglandin synthesis in fish" Proceedings on ISFーJOCS world congress. II. 1269-1275 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Matsumoto,N.Iijima,and M.Kayama: "The prostaglandin synthesis in marine fish thrombocyte" Comp.Biochem.Physiol.98B. 697-402 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Kayama,H.Matsumoto,T.Hada,J.Hirano,and T.Funada: "Comparison of aggregation behaviors in human and fish blood" Nippon Suisan Gakkaishi. 55. 697-701 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] N.Iijima,T.Hada,M.Kayama,H.Matsumoto,N. Ueda,and S.Yamamoto: "Production of monohydroxy derivatives of pylyunsaturated fatty acids in the gills of red sea bream, Pagrus major"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kayama, N. Iijima, T. Sado, H. Matsumoto, and T. Hada: "Prostaglandin synthesis in fish" Proceedings on ISF-JOCS world congress. vol. II. 1269-1275 (1289)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Matsumoto, N. Iijima, and M. Kayama: "The prostaglandin synthesis in marine fish trombocytes" Comp. Biochem. Physiol.98B. 397-402 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kayama, H. Matsumoto T. Hada, J. Hirano, and T. Funada: "Comparison of aggregation behaviors in human and fish blood" Nippon Suisan Gakkaishi. 55. 697-701 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] N. Iijima, T. Hada, H. Matsumoto, N. Ueda, and s. Yamamoto: "Production of monohydroxy derivatives of polyunsaturated fatty acids in the gills of red sea bream"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Kayama,N.Iijima,T.Sado,H.Matsumoto,and T.Hada: "Prostaglandin synthesis in fish" Proceedings on ISFーJOCS world congress. II. 1269-1275 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Matsumoto,N.Iijima,and M.Kayama: "The prostaglandin synthesis in marine fish thrombocyte" Comp.Biochem.Physiol.98B. 697-402 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Kayama,H.Matsumoto,T.Hada,J.Hirano,and T.Funaba: "Comparison of aggregation behaviors in human and fish blood" Nippon Suisan Gakkaishi. 55. 697-701 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Iijima,T,Hada,M.Kayama,H.Matsumoto,N.Ueda,and S.Yamamoto: "Production of monohydroxy derivatives of pylyunsaturated fatty acids in the gills of red sea bream,Pagrus major"

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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