| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1989: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Research Abstract |
Monoclinal antibodies HNK-1 and VC1.1 were shown to outline selectively a subpopulation of GABAergic neurons containing a specific calcium-binding protein parvalbumin (PV) in the adult rat parietal cortex, using pre- and post-embedding immunocytochemistry at light microscopic level. About 98% of HNK-1 stained cells in the rat parietal cortex were PV immunoreactive. About 95% of HNK-1 immunoreactive cells were also shown to be stained with a lectin, Vicia villosa agglutinin (VVA), with a specific affinity for terminal N-acetylgalactosamine, Previously shown to selectively stain a population of PV-containing GABAergic neurons in this region. Furthermore it was shown that almost all of HNK-1 immunoreactive cells were also stained with a monoclinal antibody 3B3, which has been established to be specific to chondroitin sulfate proteoglycan and was shown in the present study to selectively label a population of PV-immunoreative neurons in the adult rat parietal cortex. In addition, a direct comparison of two monoclonal antibodies HNK-1 and VC1.1 revealed that both were identical in their staining properties and that they defined the same subset of PV-containing GABAergic neurons in the rat rietal cortex. In the rodent cortex PV-containing neurons might be a distinctive subpopulation of GABAergic neurons, and, furthermore, specific subsets of cerebral PV-containing GABAergic neurons might be considered to have a particular microenvironment characterized by those molecular probes.
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