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Optical mapping of functional organization in the early embryonic brain stem using multiple-site recording of neural activity together with voltage-sensitive dyes.

Research Project

Project/Area Number 01480120
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KAMINO Kohtaro  Tokyo Medical and Dental University, School of Medicine, Professor., 医学部, 教授 (40025630)

Co-Investigator(Kenkyū-buntansha) KOMURO Hitoshi  Tokyo Medical and Dental University, School of Medicine, Instructor., 医学部, 助手 (40195863)
SAKAI Tetsuro  Tokyo Medical and Dental University, School of Medicine, Lecturer., 医学部, 講師 (40153845)
HIROTA Akihiko  Tokyo Medical and Dental University, School of Medicine, Assistant Professor., 医学部, 助教授 (50156717)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1990: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1989: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordsvoltage-sensitive dye / optical recording / embryonic brain stem / functional organization / vagus nerve / neural activity / glutaminergic EPSP / mapping / ダルタミン酸 / メロシアニンロ-ダニン
Research Abstract

In intact and slice preparations of the vagus/brain stem isolated from 3-to 8-day old chick embryos, the spatial pattern of neural responses to vagal stimulation and its development were assessed by using multiple-site optical recording of electrical activity, with a voltage-sensitive merocyanine-rhodanine dye (NK2761) and a 12 x 12-element photodiode array. Voltage-related optical (absorbance) changes evoked by vagus nerve stimulation with depolarizing (positive) square current pulses using a suction electrode were recorded simultaneously from 127 contiguous loci in the preparation. The measurements were made by successive displacements of the photodiode array over the image of the preparation.
The first neural responses, viz., fast optical signals related to the action potentials were recorded in the 4-day old brain stem preparations, and the slow optical signals were detected from late 7- and 8-day old brain stem preparations. The size of the slow signal was decreased by continuous s … More timulation, reduced by low external calcium ion concentrations and eliminated in the presence of manganese or cadmium ion. The slow signals were eliminated in the presence of kynurenic acid, and they were reduced by 2-APV (DL-2-amino-5-phosphono-valeric acid) and by CNQX (6-cyano-7nitroquinoxaline-2, 3-dione). We concluded that the slow signals correspond to excitatory postsynaptic potentials which are glutamate-mediated.
The evoked optical signals appeared to be concentrated longitudinally in the central region on the stimulated side of the intact brain stem preparations and in a limited dorsal area in the slice preparations. The signal size gradually increased and the response area expanded as development proceeded. Based on the above results, we have constructed developmental maps of spatial patterns of the fast and slow responses. In the maps, the positions of the peak-size area of the fast and slow signals were assessed, and we have found that there were differences in the relative locations of the peak-size regions between the fast and slow signals in the late 7- and 8-day old embryonic brain stem preparations. The fast signal response area seems to correspond to the dorsal motor nucleus of the vagus nerve and the slow signal response area to the nucleus tractus solitarii. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] K.Kamino: "Optical approaches to ontogeny of electrical activity and related functional organization during early heart development." Physiological Reviews. 71. 53-91 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Sakai: "Optical determination of impulse conduction velocity during development of embryonic chick cervical vagus nerve bundles." Journal of Physiology (London). 439. 361-381 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Komuro: "Optical detection of postsynaptic potentials evoked by vagal stimulation in the errly embryonic chick brain stem slice." Journal of Physiology (London). 442. 631-648 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Momose-Sato: "Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem." Journal of Physiology (London). 442. 649-668 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Kamino: "Optical assessment of spatially ordered patterns of neural response to vagal stimulation in the early embryonic chick brainstem." Neuroscience Research. 8. 225-271 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Kamino: "Optical studies of early developing cardiac and neural activities using voltage-sensitive dyes." Japanese Journal of Physiology. 40. 443-461 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 神野 耕太郎: "神経科学レビュ-5" 医学書院,東京 伊藤正男,楢林博太郎編, 337 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 神野 耕太郎: "脳ー可塑性研究の進歩" 朝倉書店,東京 久保田競,水野昇,山本長三郎編, 332 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K., Kato, Y., Komuro, H. & Sato, K.: "Multiple-site optical monitoring of neural activity evoked by vagus nerve stimulation in the embryonic chick brain stem." Journal of Physiology (London). 409. 263-283 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K., Hirota, A. & Komuro, H.: "Optical indications of electrical activity and excitation-contraction coupling in the early embryonic heart." Advances in Biophysics. 25. 45-93 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K., Komuro, H., Sakai, T. & Sato, K.: "Optical assessment of spatially ordered patterns of neural response to vagal stimulation in the early embryonic chick brainstem." Neuroscience Research. 8. 255-271 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K., Komuro, H., Sakai, T. & Hirota, A.: "Optical indications of spontaneous electrical activity and functional organization of pacemaking area in the early embryonic chick heart." Annals of the New York Academy of Sciences. 588. 397-400 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K.: "Optical studies of early developing cardiac and neural activities using voltage-sensitive dyes." Japanese Journal of Physiology. 40. 443-461 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K.: "Optical approaches to ontogeny of electrical activity and related functional organization during early heart development." Physiological Reviews. 71. 53-91 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai, T., Komuro, H., Katoh, Y., Sasaki, H., Momose-Sato, Y. & Kamino, K.: "Optical determination of impulse conduction velocity during development of embryonic chick cervical vagus nerve bundles." Journal of Physiology (London). 439. 361-381 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Komuro, H., Sakai, T., Momose-Sato, Y., Hirota, A. & Kamino, K.: "Optical detection of postsynaptic potentials evoked by vagal stimulation in the early embryonic chick brain stem slice." Journal of Physiology (London). 442. 631-648 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Momose-Sato, Y., Sakai, T., Komuro, H., Hirota, A. & Kamino, K.: "Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem." Journal of Physiology (London). 442. 649-668 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Momose-Sato, Y., Komuro, H., Sakai, T., Hirota, A. & Kamino, K.: "Optical monitoring of cholinergic postsynaptic potential in the embryonic chick ciliary ganglion using a voltage sensitive dye." Biomedical Research 12 Supplement. 2. 139-140 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kamino, K., Komuro, H., Sakai, T. & Hirota, A.: "Optical assessment of functional organization of pacemaking area in the early precontractile chick heart" In Developmental Cardiology : Morphogenesis and Function.Futura Press, Mount Kisco. 257-271 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Kamino: "Optical approaches to ontogeny of electrical activity and related functional organization during early heart development." Physiological Reviews. 71. 53-91 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Sakai: "Optical determination of impulse conduction velocity during development of embryonic chich cervical vagus nerve bundles." Journal of Physiology(London). 439. 361-381 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Komuro: "Optical detection of postsynaptic potentials evoked by vagal stimulation in the early embryonic chick brain stem slice." Journal of Physiology(London). 442. 631-648 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.MomoseーSato: "Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem." Journal of Physiology(London). 442. 649-668 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.MomoseーSato: "Optical monitoring of cholinergic postsynaptic potential in the embryonic chick ciliary ganglion using a voltageーsensitive dye." Biomedical Research. 12suppl.2. 139-140 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.MomoseーSato: "Optical assessment of spatial patterning of neural responsiveness to vagal stimulation in the embryonic chick brain stem." Journal of Physiology(London).

    • Related Report
      1991 Annual Research Report
  • [Publications] 神野 耕太郎: "神経科学レビュ-5" 医学書院,東京 伊藤 正男,楢林 博太郎編, 337 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Kamino: "Optical assessment of spatially ordered patterns of neural response to vagal stimulation in the early embryonic chick brainstem." Neuroscience Research. 8. 255-271 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Kamino: "Optical studies of early developing cardiac and neural activities using voltageーsensitive dyes." Japanese Journal of Physiology. 40. 443-461 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Sakai: "Optical determination of impulse conduction velocity during development of the embryonic chick cervical vagus nerve bundle." Journal of Physiology (London). (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Komuro: "Optical evidence for spontaneous electrical activity in the embryonic chick brain stem revealed by a potentiometric dye." Journal of Physiology (London).

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Komuro: "Optical detection of post synaptic potentials evoked by vagal stimulation in the early embryonic chick brain stem slice." Journal of Physiology (London).

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.MomoseーSato: "Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem." Journal of Physiology (London).

    • Related Report
      1990 Annual Research Report
  • [Publications] 神野 耕太郎: "脳のレセプタ-と運動" 平凡社,東京 永津俊治,吉田充男,金沢一郎,小川紀雄編, 242 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 神野 耕太郎: "脳ー可塑性研究の進歩" 朝倉書店,東京 久保田競,水野昇,山本長三郎編, 332 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kohtaro Kamino: "Multiple-site optical monitoring of neural activity evoked by vagus nerve stimulation in the embryonic chick brain stem" Journal of Physiology. 409. 263-283 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kohtaro Kamino: "Optical assessment of spatially ordered patterns of neural response to vagal stimulation in the early embryonic chick brain stem." Neuroscience Reserch. 10. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 神野耕太郎: "膜電位感受性色素とその細胞の電気的活動測定への適用" 電気化学および工業物理化学. 57. 881-890 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 神野耕太郎: "中枢神経系ニュ-ロン活動の光学的リアルタイムマッピング" 病態整理. 8. 418-426 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 神野耕太郎: "膜電位感受性色素を用いた活動電位の光学的測定" 細胞工学. 8. 820-828 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 神野耕太郎: "膜電位顕微測光による細胞興奮の観測" 病態整理. 9. 95-100 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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