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Phenotypic Plasticity of Grafted Catecholaminergic Neurons and its Regulatory Mechanisms.

Research Project

Project/Area Number 01480127
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionNagoya City University

Principal Investigator

NISHINO Hitoo  Nagoya City University Medical School, Department of Physiology, Professor, 医学部, 教授 (60073730)

Co-Investigator(Kenkyū-buntansha) HASHITANI Takeshi  Nagoya City University Medical School, Department of Physiology, Instructor, 医学部, 助手 (30172852)
ISOBE Yoshiaki  Nagoya City University Medical School, Department of Physiology, Instructor, 医学部, 助手 (70094357)
FURUYAMA Fujiya  Nagoya City University Medical School, Department of Physiology, Instructor, 医学部, 助手 (00080101)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsneuronal grafting / Parkinson's disease / dopamine / trophic factor / Phenotypic plasticity / striatum / substantia nigra / 移植 / カテコラミン / 表現型 / レセプタ- / mRNA / タンパク
Research Abstract

1. Using model rats of hemi-parkinson's disease, the mechanisms of functional recovery after grafting of dopaminergic cells were investigated. We found : 1) the survival of TH mRNA/TH positive cells, 2) increase of dopamine synthesis/release, 3) synaptic connections, 4) recovery of dopamine transporter and 5) normalization of D_1 and D_2 receptor-bindings.
2. Bovine total ganglioside (tGS), sialosyl cholesterol (ganglioside analogue), IL-6 (B cell stimulating cytokine) facilitated the development/growth of grafted dopaminergic cells, and promoted the motor recovery.
3.1) grafted chromaffin (adrenergic) cells transmute to dopaminergic neurons in dopamine-depleted striatum, 2) grafted noradrenergic cells transmute to dopaminergic cells in dopa-mine-depleted striatum but not in the lateral ventricle, 3) dopaminergic neurons grow much better in dopamine-depleted striatum than in normal striatum or the cortex, 4) grafted cholinergic neurons grow in a similar fashion in normal and dopamine-depleted striatum, 5) co-cultured midbrain neurons (partly dopaminergic neurons) extend more neurites faster toward the dopamine-depleted than toward the intact striatal tissue. These evidences suggest the increase in synthesis/release of some trophic factors for dopaminergic neurons in dopamine-depleted striatum.
4. In soluble fraction of dopamine-depleted striatal tissue a few acidic proteins increased. The purification/identification of these proteins is under way.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Nishino,H.,Hashitani,T.and Kumazaki,M.: "Phenotypic plasticity of grafted catecholaminergic cells in the dopamineーdepleted caudate nucleus in the rats." Neurosci.Res.,Suppl.13. S54-60 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Phenotypic plasticity of locus coeruleus noradrenergic neurons after transplantarion into the dopamineーdepleted caudate in the rat." Prog.Brain Res.82. 515-521 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ishida,Y.,Hashitani,T.,Kumazaki,M.et al.: "Behavioral and biochemical effects of intraーaccumbens dopaminergic grafts." Brain Res.Bull.24. 487-492 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.,Hashitani,T.,Isobe Y.et al.: "cGS ganglioside induces peculiar morphological features in grafted dopaminergic cells and promotes motor recovery in rats with unilateral lesions in the nigrostriatal dopamine pathway." Brain Res.534. 73-82 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Longーterm survival of grafted cells,dopamine synthesis/release,synaptic connections,and functional recovery after transplantation of fetal nigral cells in trats with unilateral 6ーOHDA lesions in the nigrostriatal dopamine pathway." Brain Res. 534. 83-93 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Grafting of catecholaminergic cells in the mammalian brain and reconstruction of disturbed function:basic problems to be solved." Comp.Biochem.Physiol.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Elsevier" Bioinformatics., 3 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.Hashitani,T.,Kumazaki,M.et al.: "Senile neurodegeneration and neurotansmitters" Excerpta Medica., 10 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino,H.,Hashitani,T.and Kumazaki,M.: "Phenotypic plasticity of grafted catecholaminergic cells in the dopamineーdepleted caudate nucleus in the rats." Neurosci.Res.,Suppl.13. S54-60 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Phenotypic plasticity of locus coeruleus noradrenergic neurons after transplantation into the dopamineーdepleted caudate im the rat." Prog.Brain Res.82. 515-521 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ishida,Y.,Hashitani,T.,Kumazaki,M.et al.: "Behavioral and biochemical effects of intraーaccumbens dopaminergic grafts." Brain Res.Bull.24. 487-492 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.,Hashitani,T.,Isobe Y.et al.: "tGS ganglioside induces peculiar morphological features in grafted dopaminergic cells and promotes motor recovery in rats with unilateral lesions in the nigrostriatal dopamine pathway." Brain Res.534. 73-82 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Longーterm survival of grafted cells,dopamine synthesis/release,synaptic connections,and functional recovery after transplantation of fetal nigral cells in trats with unilateral 6ーOHDA lesions in the nigrostriatal dopamine pathway." Brain Res.534. 83-93 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Grafting of catecholaminergic cells in the mammalian brain and reconstruction of disturbed function:basic problems to be solved." Comp.Biochem.Physiol.(in press). (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Elsevier" Bioinformatics., 3 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.Hashitani,T.,Kumazaki,M.et al.: "Senile neurodegeneration and neurotansmitters" Excerpta Medica,10 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani,M.Kumazaki,Y.Ishida,et al.: "Phenotypic plasticity of locus coeruleus noradrenergic neurons after transplantation into the dopamine depleted caudate in the rat." Prog.Brain Res.82. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Ishida,T.Hashitani,M.Kumazaki,T.Ikeda & H.Nishino: "Behavioral and biochemical effects of intra-accumbens dopaminergic grafts." Brain Res.Bull.24. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani & M.Kumazaki: "Grafting of catecholaminergic cells in the mammalian brain and reconstruction of disturbed function:basic problems to be solved." Comp.Biochem.Physiol.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani & M.Kumazaki: "Phenotypic plasticity of grafted catecholaminergic cells in dopamine-depleted caudate in the rat." Neurosci.Res.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani,M.Kumazaki,H.Sato,F.Furuyama,Y.Isobe et al: "Long-term survival of grafted cells,connection with hostneurons,dopamine synthesis/release,and functional recovery after transplantation of fetal nigral cells in rats." Brain Res.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani,Y.Isobe,F.Furuyama,H.Sato et al: "tGS ganglioside facilitates development of grafted nigral dopaminergic cells and promotes motor recovery in rats." Brain Res.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani,M.Kumazaki,Y.Ishida,F.Furuyama,Y.Isobe & H.Sato: "Bioinformatics" Elsevier Science Publisher, pp.319-322 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Nishino,T.Hashitani,K.Mizukawa,N.Ogawa & S.Shiosaka: "Alzheimer's and Parkinson's Diseases II:Basic and Therapeutic Strategies" Plenum, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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